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UniProtKB - P16951 (ATF2_MOUSE)

Protein

Cyclic AMP-dependent transcription factor ATF-2

Gene

Atf2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional activator which regulates the transcription of various genes, including those involved in anti-apoptosis, cell growth, and DNA damage response. Dependent on its binding partner, binds to CRE (cAMP response element) consensus sequences (5'-TGACGTCA-3') or to AP-1 (activator protein 1) consensus sequences (5'-TGACTCA-3'). In the nucleus, contributes to global transcription and the DNA damage response, in addition to specific transcriptional activities that are related to cell development, proliferation and death. In the cytoplasm, interacts with and perturbs HK1- and VDAC1-containing complexes at the mitochondrial outer membrane, thereby impairing mitochondrial membrane potential, inducing mitochondrial leakage and promoting cell death. The phosphorylated form (mediated by ATM) plays a role in the DNA damage response and is involved in the ionizing radiation (IR)-induced S phase checkpoint control and in the recruitment of the MRN complex into the IR-induced foci (IRIF). Exhibits histone acetyltransferase (HAT) activity which specifically acetylates histones H2B and H4 in vitro. In concert with CUL3 and RBX1, promotes the degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. Can elicit oncogenic or tumor suppressor activities depending on the tissue or cell type (By similarity).By similarity

Catalytic activityi

Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N6-acetyl-L-lysine.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri7 – 31C2H2-typePROSITE-ProRule annotationAdd BLAST25

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

GO - Biological processi

View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionActivator, DNA-binding, Transferase
Biological processDNA damage, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-3214847 HATs acetylate histones
R-MMU-450341 Activation of the AP-1 family of transcription factors
R-MMU-9018519 Estrogen-dependent gene expression

Protein family/group databases

MoonProtiP16951

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclic AMP-dependent transcription factor ATF-2 (EC:2.3.1.48By similarity)
Short name:
cAMP-dependent transcription factor ATF-2
Alternative name(s):
Activating transcription factor 2
MXBP protein
cAMP response element-binding protein CRE-BP1
Gene namesi
Name:Atf2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:109349 Atf2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane, Nucleus

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2176797

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000765781 – 487Cyclic AMP-dependent transcription factor ATF-2Add BLAST487

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei34Phosphothreonine; by PKC/PRKCHBy similarity1
Modified residuei44PhosphoserineCombined sources1
Modified residuei51Phosphothreonine; by MAPK11 and MAPK14Combined sources1
Modified residuei53Phosphothreonine; by MAPK1, MAPK3, MAPK11, MAPK12, MAPK14 and PLK3Combined sources1
Modified residuei55Phosphothreonine; by VRK1By similarity1
Modified residuei72PhosphoserineCombined sources1
Modified residuei94PhosphoserineCombined sources1
Modified residuei98PhosphothreonineBy similarity1
Modified residuei103Phosphoserine; by PKC/PRKCA and PKC/PRKCBBy similarity1
Modified residuei118PhosphoserineBy similarity1
Modified residuei310PhosphoserineBy similarity1
Modified residuei322Phosphoserine; by PKC/PRKCA and PKC/PRKCBBy similarity1
Modified residuei339N6-acetyllysineBy similarity1
Modified residuei349Phosphoserine; by PKC/PRKCA and PKC/PRKCBBy similarity1
Modified residuei356N6-acetyllysineBy similarity1
Modified residuei424PhosphoserineBy similarity1
Modified residuei428PhosphoserineBy similarity1
Modified residuei472Phosphoserine; by ATMBy similarity1
Modified residuei480Phosphoserine; by ATMBy similarity1

Post-translational modificationi

Phosphorylation of Thr-51 by MAPK14 and MAPK11, and at Thr-53 by MAPK1/ERK2, MAPK3/ERK1, MAPK11, MAPK12 and MAPK14 in response to external stimulus like insulin causes increased transcriptional activity (By similarity). Phosphorylated by PLK3 following hyperosmotic stress. Also phosphorylated and activated by JNK and CaMK4 (By similarity). Phosphorylation at Ser-44, Thr-55 and Ser-103 activates its transcriptional activity (By similarity). ATM-mediated phosphorylation at Ser-472 and Ser-480 stimulates its function in DNA damage response. Phosphorylation at Thr-51 or Thr-53 enhances its histone acetyltransferase (HAT) activity.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP16951
PaxDbiP16951
PeptideAtlasiP16951
PRIDEiP16951

PTM databases

iPTMnetiP16951
PhosphoSitePlusiP16951

Expressioni

Gene expression databases

BgeeiENSMUSG00000027104 Expressed in 315 organ(s), highest expression level in paraventricular nucleus of hypothalamus
CleanExiMM_ATF2
ExpressionAtlasiP16951 baseline and differential
GenevisibleiP16951 MM

Interactioni

Subunit structurei

Binds DNA as a dimer and can form a homodimer in the absence of DNA. Can form a heterodimer with JUN. Heterodimerization is essential for its transcriptional activity. Interacts with SMAD3 and SMAD4. Interacts with the HK1/VDAC1 complex. Interacts with NBN, MRE11, XPO1, KAT5 and CUL3 (By similarity). Binds through its N-terminal region to UTF1 which acts as a coactivator of ATF2 transcriptional activity.By similarity1 Publication

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridi198233, 15 interactors
IntActiP16951, 5 interactors
MINTiP16951
STRINGi10090.ENSMUSP00000058521

Structurei

3D structure databases

ProteinModelPortaliP16951
SMRiP16951
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini334 – 397bZIPPROSITE-ProRule annotationAdd BLAST64

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni278 – 281Essential for its histone acetyltransferase activityBy similarity4
Regioni336 – 356Basic motifPROSITE-ProRule annotationAdd BLAST21
Regioni362 – 390Leucine-zipperPROSITE-ProRule annotationAdd BLAST29

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi387 – 396Nuclear export signalBy similarity10

Sequence similaritiesi

Belongs to the bZIP family. ATF subfamily.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri7 – 31C2H2-typePROSITE-ProRule annotationAdd BLAST25

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG1414 Eukaryota
ENOG4111CH5 LUCA
GeneTreeiENSGT00390000020106
HOGENOMiHOG000220894
HOVERGENiHBG004300
InParanoidiP16951
KOiK04450
OMAiHPEAIQH
OrthoDBiEOG091G0AO8
PhylomeDBiP16951

Family and domain databases

InterProiView protein in InterPro
IPR029836 ATF2
IPR004827 bZIP
IPR016378 TF_CRE-BP1-typ
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PANTHERiPTHR19304:SF9 PTHR19304:SF9, 1 hit
PfamiView protein in Pfam
PF00170 bZIP_1, 1 hit
PIRSFiPIRSF003153 ATF2_CRE-BP1, 1 hit
SMARTiView protein in SMART
SM00338 BRLZ, 1 hit
SUPFAMiSSF57667 SSF57667, 1 hit
PROSITEiView protein in PROSITE
PS50217 BZIP, 1 hit
PS00036 BZIP_BASIC, 1 hit
PS00028 ZINC_FINGER_C2H2_1, 1 hit
PS50157 ZINC_FINGER_C2H2_2, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P16951-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSDDKPFLCT APGCGQRFTN EDHLAVHKHK HEMTLKFGPA RNDSVIVADQ 
        60         70         80         90        100
TPTPTRFLKN CEEVGLFNEL ASPFENEFKK ASEDDIKKMP LDLSPLATPI 
       110        120        130        140        150
IRSKIEEPSV VETTHQDSPL PHPESTTSDE KEVPLAQTAQ PTSAIVRPAS 
       160        170        180        190        200
LQVPNVLLTS SDSSVIIQQA VPSPTSSTVI TQAPSSNRPI VPVPGPFPLL 
       210        220        230        240        250
LHLPNGQTMP VAIPASITSS NVHVPAAVPL VRPVTMVPSV PGIPGPSSPQ 
       260        270        280        290        300
PVQSEAKMRL KAALTQQHPP VTNGDTVKGH GSGLVRTQSE ESRPQSLQQP 
       310        320        330        340        350
ATSTTETPAS PAHTTPQTQN TSGRRRRAAN EDPDEKRRKF LERNRAAASR 
       360        370        380        390        400
CRQKRKVWVQ SLEKKAEDLS SLNGQLQSEV TLLRNEVAQL KQLLLAHKDC 
       410        420        430        440        450
PVTAMQKKSG YHTADKDDSS EDLSVPSSPH TEAIQHSSVS TSNGVSSTSK 
       460        470        480 
AEAVATSVLT QMADQSTEPA LSQIVMAPPS QAQPSGS
Length:487
Mass (Da):52,298
Last modified:May 30, 2000 - v2
Checksum:iF9CDEC3BC3119ACB
GO
Isoform 2 (identifier: P16951-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     132-229: Missing.

Show »
Length:389
Mass (Da):42,345
Checksum:i770AD65EA6114F10
GO
Isoform 3 (identifier: P16951-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-48: MSDDKPFLCTAPGCGQRFTNEDHLAVHKHKHEMTLKFGPARNDSVIVA → MHCPWVWP

Show »
Length:447
Mass (Da):48,002
Checksum:i35A0751C1627D682
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F6Z2B2F6Z2B2_MOUSE
Cyclic AMP-dependent transcription ...
Atf2
212Annotation score:
G3UXC3G3UXC3_MOUSE
Cyclic AMP-dependent transcription ...
Atf2
377Annotation score:
A2AQE9A2AQE9_MOUSE
Cyclic AMP-dependent transcription ...
Atf2
207Annotation score:
A2AQF0A2AQF0_MOUSE
Cyclic AMP-dependent transcription ...
Atf2
228Annotation score:
A2AQF1A2AQF1_MOUSE
Cyclic AMP-dependent transcription ...
Atf2
146Annotation score:

Sequence cautioni

The sequence AAB21128 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAB21129 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti482 – 487AQPSGS → HSPQEVD in AAB21128 (PubMed:11471062).Curated6
Sequence conflicti482 – 487AQPSGS → HSPQEVD in AAB21129 (PubMed:11471062).Curated6

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0005891 – 48MSDDK…SVIVA → MHCPWVWP in isoform 3. CuratedAdd BLAST48
Alternative sequenceiVSP_000590132 – 229Missing in isoform 2. CuratedAdd BLAST98

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF483482 mRNA Translation: AAL90756.1
AF483483 mRNA Translation: AAL90757.1
S76657 mRNA Translation: AAB21128.1 Different initiation.
S76659 mRNA Translation: AAB21129.1 Different initiation.
S76655 mRNA Translation: AAB21127.1
M31629 mRNA Translation: AAA39780.1
CCDSiCCDS16134.1 [P16951-1]
CCDS16135.1 [P16951-3]
CCDS71076.1 [P16951-2]
PIRiA42026
C42026
RefSeqiNP_001020264.1, NM_001025093.2 [P16951-1]
UniGeneiMm.209903

Genome annotation databases

EnsembliENSMUST00000055833; ENSMUSP00000058521; ENSMUSG00000027104 [P16951-1]
ENSMUST00000090802; ENSMUSP00000088311; ENSMUSG00000027104 [P16951-3]
ENSMUST00000100009; ENSMUSP00000097588; ENSMUSG00000027104 [P16951-1]
ENSMUST00000112007; ENSMUSP00000107638; ENSMUSG00000027104 [P16951-3]
ENSMUST00000112010; ENSMUSP00000107641; ENSMUSG00000027104 [P16951-3]
ENSMUST00000112016; ENSMUSP00000107647; ENSMUSG00000027104 [P16951-2]
ENSMUST00000112017; ENSMUSP00000107648; ENSMUSG00000027104 [P16951-1]
GeneIDi11909
KEGGimmu:11909
UCSCiuc008kdd.2 mouse [P16951-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF483482 mRNA Translation: AAL90756.1
AF483483 mRNA Translation: AAL90757.1
S76657 mRNA Translation: AAB21128.1 Different initiation.
S76659 mRNA Translation: AAB21129.1 Different initiation.
S76655 mRNA Translation: AAB21127.1
M31629 mRNA Translation: AAA39780.1
CCDSiCCDS16134.1 [P16951-1]
CCDS16135.1 [P16951-3]
CCDS71076.1 [P16951-2]
PIRiA42026
C42026
RefSeqiNP_001020264.1, NM_001025093.2 [P16951-1]
UniGeneiMm.209903

3D structure databases

ProteinModelPortaliP16951
SMRiP16951
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198233, 15 interactors
IntActiP16951, 5 interactors
MINTiP16951
STRINGi10090.ENSMUSP00000058521

Chemistry databases

ChEMBLiCHEMBL2176797

Protein family/group databases

MoonProtiP16951

PTM databases

iPTMnetiP16951
PhosphoSitePlusiP16951

Proteomic databases

MaxQBiP16951
PaxDbiP16951
PeptideAtlasiP16951
PRIDEiP16951

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000055833; ENSMUSP00000058521; ENSMUSG00000027104 [P16951-1]
ENSMUST00000090802; ENSMUSP00000088311; ENSMUSG00000027104 [P16951-3]
ENSMUST00000100009; ENSMUSP00000097588; ENSMUSG00000027104 [P16951-1]
ENSMUST00000112007; ENSMUSP00000107638; ENSMUSG00000027104 [P16951-3]
ENSMUST00000112010; ENSMUSP00000107641; ENSMUSG00000027104 [P16951-3]
ENSMUST00000112016; ENSMUSP00000107647; ENSMUSG00000027104 [P16951-2]
ENSMUST00000112017; ENSMUSP00000107648; ENSMUSG00000027104 [P16951-1]
GeneIDi11909
KEGGimmu:11909
UCSCiuc008kdd.2 mouse [P16951-1]

Organism-specific databases

CTDi1386
MGIiMGI:109349 Atf2

Phylogenomic databases

eggNOGiKOG1414 Eukaryota
ENOG4111CH5 LUCA
GeneTreeiENSGT00390000020106
HOGENOMiHOG000220894
HOVERGENiHBG004300
InParanoidiP16951
KOiK04450
OMAiHPEAIQH
OrthoDBiEOG091G0AO8
PhylomeDBiP16951

Enzyme and pathway databases

ReactomeiR-MMU-3214847 HATs acetylate histones
R-MMU-450341 Activation of the AP-1 family of transcription factors
R-MMU-9018519 Estrogen-dependent gene expression

Miscellaneous databases

ChiTaRSiAtf2 mouse
PROiPR:P16951
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027104 Expressed in 315 organ(s), highest expression level in paraventricular nucleus of hypothalamus
CleanExiMM_ATF2
ExpressionAtlasiP16951 baseline and differential
GenevisibleiP16951 MM

Family and domain databases

InterProiView protein in InterPro
IPR029836 ATF2
IPR004827 bZIP
IPR016378 TF_CRE-BP1-typ
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PANTHERiPTHR19304:SF9 PTHR19304:SF9, 1 hit
PfamiView protein in Pfam
PF00170 bZIP_1, 1 hit
PIRSFiPIRSF003153 ATF2_CRE-BP1, 1 hit
SMARTiView protein in SMART
SM00338 BRLZ, 1 hit
SUPFAMiSSF57667 SSF57667, 1 hit
PROSITEiView protein in PROSITE
PS50217 BZIP, 1 hit
PS00036 BZIP_BASIC, 1 hit
PS00028 ZINC_FINGER_C2H2_1, 1 hit
PS50157 ZINC_FINGER_C2H2_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiATF2_MOUSE
AccessioniPrimary (citable) accession number: P16951
Secondary accession number(s): Q64089, Q64090, Q64091
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: May 30, 2000
Last modified: November 7, 2018
This is version 182 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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