UniProtKB - P16892 (FUS3_YEAST)
Mitogen-activated protein kinase FUS3
FUS3
Functioni
Together with closely related KSS1, FUS3 is the final kinase in the signal transduction cascade regulating activation/repression of the mating and filamentation pathways, induced by pheromone and nitrogen/carbon limitation, respectively. Phosphorylated FUS3 activates the mating but suppresses the filamentation pathway, whereas activated KSS1 activates both pathways. Pheromone-activated FUS3 functions by inhibiting the binding of the transcriptional activator STE12 to filamentation specific genes while inducing its binding to and activity at mating specific genes. Non-activated FUS3 has a repressive effect on STE12 transcriptional activity. KSS1 can partially compensate for the lack of FUS3 but mating efficiency is reduced and the filamentation program is partially activated upon pheromone signaling. FUS3 phosphorylates STE7, STE5, FAR1, DIG1, DIG2 and STE12.
4 PublicationsMiscellaneous
Catalytic activityi
Cofactori
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 42 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 137 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 19 – 27 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- identical protein binding Source: IntAct
- MAP kinase activity Source: SGD
- protein kinase activity Source: SGD
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine/threonine kinase activity Source: GO_Central
- protein serine kinase activity Source: RHEA
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- cell division Source: UniProtKB-KW
- intracellular signal transduction Source: GO_Central
- invasive growth in response to glucose limitation Source: SGD
- negative regulation of MAPK cascade Source: SGD
- negative regulation of transposition, RNA-mediated Source: SGD
- pheromone-dependent signal transduction involved in conjugation with cellular fusion Source: SGD
- pheromone response MAPK cascade Source: SGD
- positive regulation of protein export from nucleus Source: SGD
- protein autophosphorylation Source: SGD
- protein phosphorylation Source: SGD
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Cell cycle, Cell division, Conjugation, Mitosis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.11.24, 984 |
Reactomei | R-SCE-110056, MAPK3 (ERK1) activation R-SCE-111995, phospho-PLA2 pathway R-SCE-112409, RAF-independent MAPK1/3 activation R-SCE-112411, MAPK1 (ERK2) activation R-SCE-170968, Frs2-mediated activation R-SCE-198753, ERK/MAPK targets R-SCE-202670, ERKs are inactivated R-SCE-3371453, Regulation of HSF1-mediated heat shock response R-SCE-375165, NCAM signaling for neurite out-growth R-SCE-437239, Recycling pathway of L1 R-SCE-445144, Signal transduction by L1 R-SCE-450341, Activation of the AP-1 family of transcription factors R-SCE-5673001, RAF/MAP kinase cascade R-SCE-5674135, MAP2K and MAPK activation R-SCE-5674499, Negative feedback regulation of MAPK pathway R-SCE-5675221, Negative regulation of MAPK pathway R-SCE-6798695, Neutrophil degranulation R-SCE-9634635, Estrogen-stimulated signaling through PRKCZ |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:FUS3 Synonyms:DAC2 Ordered Locus Names:YBL016W ORF Names:YBL03.21, YBL0303 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000000112, FUS3 |
VEuPathDBi | FungiDB:YBL016W |
Subcellular locationi
Mitochondrion
- mitochondrion Source: SGD
Nucleus
- nucleus Source: SGD
Other locations
- cytoplasm Source: SGD
- cytoplasmic stress granule Source: SGD
- mating projection tip Source: SGD
- periplasmic space Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cytoplasm, Nucleus, PeriplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000186326 | 1 – 353 | Mitogen-activated protein kinase FUS3Add BLAST | 353 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 180 | Phosphothreonine1 Publication | 1 | |
Modified residuei | 182 | Phosphotyrosine1 Publication | 1 | |
Cross-linki | 345 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources |
Post-translational modificationi
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
MaxQBi | P16892 |
PaxDbi | P16892 |
PRIDEi | P16892 |
PTM databases
iPTMneti | P16892 |
Interactioni
Subunit structurei
In the nucleus, FUS3 forms a complex with DIG1, DIG2 and STE12. The interaction of FUS3 with STE12 depends on the presence of both DIG1 and DIG2. STE12 is lost from FUS3/DIG1/DIG2 complex after pheromone treatment. During its activation and phosphorylation, FUS3 forms a membrane-associated complex with the scaffold protein STE5, the MAPKK STE7, the MAPKKK STE11, and the G-protein beta subunit GBB/STE4; interacting directly with STE7 and STE5.
Binary interactionsi
P16892
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 32682, 430 interactors |
DIPi | DIP-714N |
ELMi | P16892 |
IntActi | P16892, 35 interactors |
MINTi | P16892 |
STRINGi | 4932.YBL016W |
Miscellaneous databases
RNActi | P16892, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P16892 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P16892 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 13 – 309 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 297 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 180 – 182 | TXY | 3 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0660, Eukaryota |
HOGENOMi | CLU_000288_181_1_1 |
InParanoidi | P16892 |
OMAi | IFDIQRP |
Family and domain databases
DisProti | DP02784 |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR003527, MAP_kinase_CS IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS01351, MAPK, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MPKRIVYNIS SDFQLKSLLG EGAYGVVCSA THKPTGEIVA IKKIEPFDKP
60 70 80 90 100
LFALRTLREI KILKHFKHEN IITIFNIQRP DSFENFNEVY IIQELMQTDL
110 120 130 140 150
HRVISTQMLS DDHIQYFIYQ TLRAVKVLHG SNVIHRDLKP SNLLINSNCD
160 170 180 190 200
LKVCDFGLAR IIDESAADNS EPTGQQSGMT EYVATRWYRA PEVMLTSAKY
210 220 230 240 250
SRAMDVWSCG CILAELFLRR PIFPGRDYRH QLLLIFGIIG TPHSDNDLRC
260 270 280 290 300
IESPRAREYI KSLPMYPAAP LEKMFPRVNP KGIDLLQRML VFDPAKRITA
310 320 330 340 350
KEALEHPYLQ TYHDPNDEPE GEPIPPSFFE FDHYKEALTT KDLKKLIWNE
IFS
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 334 | Y → H in AAA34613 (PubMed:2406028).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M31132 Genomic DNA Translation: AAA34613.1 X69572 Genomic DNA Translation: CAA49292.1 X68577 Genomic DNA Translation: CAA48569.1 Z35777 Genomic DNA Translation: CAA84835.1 AY693096 Genomic DNA Translation: AAT93115.1 BK006936 Genomic DNA Translation: DAA07104.1 |
PIRi | S28548 |
RefSeqi | NP_009537.1, NM_001178256.1 |
Genome annotation databases
EnsemblFungii | YBL016W_mRNA; YBL016W; YBL016W |
GeneIDi | 852265 |
KEGGi | sce:YBL016W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M31132 Genomic DNA Translation: AAA34613.1 X69572 Genomic DNA Translation: CAA49292.1 X68577 Genomic DNA Translation: CAA48569.1 Z35777 Genomic DNA Translation: CAA84835.1 AY693096 Genomic DNA Translation: AAT93115.1 BK006936 Genomic DNA Translation: DAA07104.1 |
PIRi | S28548 |
RefSeqi | NP_009537.1, NM_001178256.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2B9F | X-ray | 1.80 | A | 1-353 | [»] | |
2B9H | X-ray | 1.55 | A | 1-353 | [»] | |
2B9I | X-ray | 2.50 | A | 1-353 | [»] | |
2B9J | X-ray | 2.30 | A | 1-353 | [»] | |
2F49 | X-ray | 1.90 | A/B | 1-353 | [»] | |
2F9G | X-ray | 2.10 | A | 1-353 | [»] | |
2FA2 | X-ray | 2.85 | A/B | 1-353 | [»] | |
SMRi | P16892 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 32682, 430 interactors |
DIPi | DIP-714N |
ELMi | P16892 |
IntActi | P16892, 35 interactors |
MINTi | P16892 |
STRINGi | 4932.YBL016W |
PTM databases
iPTMneti | P16892 |
Proteomic databases
MaxQBi | P16892 |
PaxDbi | P16892 |
PRIDEi | P16892 |
Genome annotation databases
EnsemblFungii | YBL016W_mRNA; YBL016W; YBL016W |
GeneIDi | 852265 |
KEGGi | sce:YBL016W |
Organism-specific databases
SGDi | S000000112, FUS3 |
VEuPathDBi | FungiDB:YBL016W |
Phylogenomic databases
eggNOGi | KOG0660, Eukaryota |
HOGENOMi | CLU_000288_181_1_1 |
InParanoidi | P16892 |
OMAi | IFDIQRP |
Enzyme and pathway databases
BRENDAi | 2.7.11.24, 984 |
Reactomei | R-SCE-110056, MAPK3 (ERK1) activation R-SCE-111995, phospho-PLA2 pathway R-SCE-112409, RAF-independent MAPK1/3 activation R-SCE-112411, MAPK1 (ERK2) activation R-SCE-170968, Frs2-mediated activation R-SCE-198753, ERK/MAPK targets R-SCE-202670, ERKs are inactivated R-SCE-3371453, Regulation of HSF1-mediated heat shock response R-SCE-375165, NCAM signaling for neurite out-growth R-SCE-437239, Recycling pathway of L1 R-SCE-445144, Signal transduction by L1 R-SCE-450341, Activation of the AP-1 family of transcription factors R-SCE-5673001, RAF/MAP kinase cascade R-SCE-5674135, MAP2K and MAPK activation R-SCE-5674499, Negative feedback regulation of MAPK pathway R-SCE-5675221, Negative regulation of MAPK pathway R-SCE-6798695, Neutrophil degranulation R-SCE-9634635, Estrogen-stimulated signaling through PRKCZ |
Miscellaneous databases
EvolutionaryTracei | P16892 |
PROi | PR:P16892 |
RNActi | P16892, protein |
Family and domain databases
DisProti | DP02784 |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR003527, MAP_kinase_CS IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS01351, MAPK, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | FUS3_YEAST | |
Accessioni | P16892Primary (citable) accession number: P16892 Secondary accession number(s): D6VPY4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1990 |
Last sequence update: | October 1, 1993 | |
Last modified: | February 23, 2022 | |
This is version 221 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome II
Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families