UniProtKB - P16860 (ANFB_HUMAN)
Protein
Natriuretic peptides B
Gene
NPPB
Organism
Homo sapiens (Human)
Status
Functioni
Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.1 Publication
GO - Molecular functioni
- diuretic hormone activity Source: UniProtKB
- hormone activity Source: GO_Central
- hormone receptor binding Source: UniProtKB
- signaling receptor binding Source: UniProtKB
GO - Biological processi
- antimicrobial humoral immune response mediated by antimicrobial peptide Source: UniProtKB
- body fluid secretion Source: UniProtKB
- cell surface receptor signaling pathway Source: UniProtKB
- cGMP biosynthetic process Source: GO_Central
- cGMP-mediated signaling Source: GO_Central
- killing of cells of other organism Source: UniProtKB
- negative regulation of angiogenesis Source: UniProtKB
- negative regulation of cell growth Source: UniProtKB
- negative regulation of systemic arterial blood pressure Source: GO_Central
- neuropeptide signaling pathway Source: GO_Central
- positive regulation of renal sodium excretion Source: UniProtKB
- positive regulation of urine volume Source: UniProtKB
- protein folding Source: CAFA
- receptor guanylyl cyclase signaling pathway Source: UniProtKB
- regulation of blood pressure Source: UniProtKB
- regulation of blood vessel diameter Source: UniProtKB
- regulation of vascular permeability Source: UniProtKB
Keywordsi
Molecular function | Hormone, Vasoactive |
Enzyme and pathway databases
SIGNORi | P16860 |
Names & Taxonomyi
Protein namesi | Recommended name: Natriuretic peptides BAlternative name(s): Gamma-brain natriuretic peptide Cleaved into the following 16 chains: |
Gene namesi | Name:NPPB |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000120937.8 |
HGNCi | HGNC:7940 NPPB |
MIMi | 600295 gene |
neXtProti | NX_P16860 |
Subcellular locationi
Extracellular region or secreted
Extracellular region or secreted
- extracellular region Source: UniProtKB
- extracellular space Source: GO_Central
Nucleus
- nucleus Source: GO_Central
Other locations
- cytoplasm Source: GO_Central
- protein-containing complex Source: CAFA
Keywords - Cellular componenti
SecretedPathology & Biotechi
Pharmaceutical usei
Available under the name Nesiritide (Scios). Used for the treatment of heart failure.
Organism-specific databases
DisGeNETi | 4879 |
OpenTargetsi | ENSG00000120937 |
PharmGKBi | PA31734 |
Chemistry databases
DrugBanki | DB01136 Carvedilol DB06412 Oxymetholone |
Polymorphism and mutation databases
BioMutai | NPPB |
DMDMi | 113836 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 26 | 1 PublicationAdd BLAST | 26 | |
ChainiPRO_0000001531 | 27 – 134 | Natriuretic peptides BAdd BLAST | 108 | |
PeptideiPRO_0000001532 | 103 – 134 | Brain natriuretic peptide 32Add BLAST | 32 | |
PeptideiPRO_0000364993 | 103 – 132 | BNP(1-30)Add BLAST | 30 | |
PeptideiPRO_0000364994 | 103 – 131 | BNP(1-29)Add BLAST | 29 | |
PeptideiPRO_0000364995 | 103 – 130 | BNP(1-28)Add BLAST | 28 | |
PeptideiPRO_0000364996 | 104 – 133 | BNP(2-31)Add BLAST | 30 | |
PeptideiPRO_0000364997 | 105 – 134 | BNP(3-32)Add BLAST | 30 | |
PeptideiPRO_0000364998 | 105 – 132 | BNP(3-30)Add BLAST | 28 | |
PeptideiPRO_0000364999 | 105 – 131 | BNP(3-29)Add BLAST | 27 | |
PeptideiPRO_0000365000 | 106 – 134 | BNP(4-32)Add BLAST | 29 | |
PeptideiPRO_0000365001 | 106 – 133 | BNP(4-31)Add BLAST | 28 | |
PeptideiPRO_0000365002 | 106 – 132 | BNP(4-30)Add BLAST | 27 | |
PeptideiPRO_0000365003 | 106 – 131 | BNP(4-29)Add BLAST | 26 | |
PeptideiPRO_0000365004 | 106 – 129 | BNP(4-27)Add BLAST | 24 | |
PeptideiPRO_0000365005 | 107 – 134 | BNP(5-32)Add BLAST | 28 | |
PeptideiPRO_0000365006 | 107 – 133 | BNP(5-31)Add BLAST | 27 | |
PeptideiPRO_0000365007 | 107 – 131 | BNP(5-29)Add BLAST | 25 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 62 | O-linked (HexNAc...) threonine; Partial1 Publication | 1 | |
Glycosylationi | 63 | O-linked (HexNAc...) serine1 Publication | 1 | |
Glycosylationi | 70 | O-linked (HexNAc...) serine1 Publication | 1 | |
Glycosylationi | 74 | O-linked (HexNAc...) threonine1 Publication | 1 | |
Glycosylationi | 79 | O-linked (HexNAc...) serine1 Publication | 1 | |
Glycosylationi | 84 | O-linked (HexNAc...) threonine; Partial1 Publication | 1 | |
Glycosylationi | 97 | O-linked (HexNAc...) threonine1 Publication | 1 | |
Disulfide bondi | 112 ↔ 128 | 1 Publication |
Post-translational modificationi
The brain natriuretic peptide 32 form is cleaved at Pro-104 by the prolyl endopeptidase FAP (seprase) activity (in vitro).1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 104 – 105 | Cleavage; by FAP1 Publication | 2 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | P16860 |
PeptideAtlasi | P16860 |
PRIDEi | P16860 |
ProteomicsDBi | 53393 |
PTM databases
iPTMneti | P16860 |
PhosphoSitePlusi | P16860 |
Miscellaneous databases
PMAP-CutDBi | P16860 |
Expressioni
Tissue specificityi
Brain and also in atria, but at much lower levels than ANP.
Gene expression databases
Bgeei | ENSG00000120937 Expressed in 86 organ(s), highest expression level in right atrium auricular region |
Genevisiblei | P16860 HS |
Interactioni
Binary interactionsi
GO - Molecular functioni
- diuretic hormone activity Source: UniProtKB
- hormone activity Source: GO_Central
- hormone receptor binding Source: UniProtKB
- signaling receptor binding Source: UniProtKB
Protein-protein interaction databases
BioGridi | 110939, 19 interactors |
IntActi | P16860, 10 interactors |
MINTi | P16860 |
STRINGi | 9606.ENSP00000365651 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1YK1 | X-ray | 2.90 | E | 111-131 | [»] | |
3N56 | X-ray | 3.10 | C/D | 103-134 | [»] | |
DisProti | DP00551 | |||||
ProteinModelPortali | P16860 | |||||
SMRi | P16860 | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P16860 |
Family & Domainsi
Sequence similaritiesi
Belongs to the natriuretic peptide family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | ENOG410J2F8 Eukaryota ENOG410ZE1B LUCA |
GeneTreei | ENSGT00940000154513 |
HOGENOMi | HOG000033937 |
HOVERGENi | HBG078206 |
InParanoidi | P16860 |
KOi | K12335 |
OMAi | GCNVLRR |
OrthoDBi | 1493586at2759 |
PhylomeDBi | P16860 |
TreeFami | TF106304 |
Family and domain databases
InterProi | View protein in InterPro IPR000663 Natr_peptide IPR030480 Natr_peptide_CS IPR002408 Natriuretic_peptide_brain |
Pfami | View protein in Pfam PF00212 ANP, 1 hit |
PRINTSi | PR00712 BNATPEPTIDE |
SMARTi | View protein in SMART SM00183 NAT_PEP, 1 hit |
PROSITEi | View protein in PROSITE PS00263 NATRIURETIC_PEPTIDE, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P16860-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDPQTAPSRA LLLLLFLHLA FLGGRSHPLG SPGSASDLET SGLQEQRNHL
60 70 80 90 100
QGKLSELQVE QTSLEPLQES PRPTGVWKSR EVATEGIRGH RKMVLYTLRA
110 120 130
PRSPKMVQGS GCFGRKMDRI SSSSGLGCKV LRRH
Sequence cautioni
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_014580 | 25 | R → L. Corresponds to variant dbSNP:rs5227Ensembl. | 1 | |
Natural variantiVAR_014581 | 47 | R → H. Corresponds to variant dbSNP:rs5229Ensembl. | 1 | |
Natural variantiVAR_014582 | 93 | M → L. Corresponds to variant dbSNP:rs5230Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M31776 Genomic DNA Translation: AAA35603.1 M25296 mRNA Translation: AAA36355.1 AB037521 Genomic DNA Translation: BAA90441.1 Frameshift. CR541976 mRNA Translation: CAG46774.1 CR542003 mRNA Translation: CAG46800.1 EU326309 Genomic DNA Translation: ACA05917.1 AL021155 Genomic DNA No translation available. CH471130 Genomic DNA Translation: EAW71718.1 BC025785 mRNA Translation: AAH25785.1 |
CCDSi | CCDS140.1 |
PIRi | A36736 AWHUB |
RefSeqi | NP_002512.1, NM_002521.2 |
UniGenei | Hs.219140 |
Genome annotation databases
Ensembli | ENST00000376468; ENSP00000365651; ENSG00000120937 |
GeneIDi | 4879 |
KEGGi | hsa:4879 |
UCSCi | uc001atj.4 human |
Keywords - Coding sequence diversityi
PolymorphismSimilar proteinsi
Cross-referencesi
Web resourcesi
Wikipedia Brain natriuretic peptide entry |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M31776 Genomic DNA Translation: AAA35603.1 M25296 mRNA Translation: AAA36355.1 AB037521 Genomic DNA Translation: BAA90441.1 Frameshift. CR541976 mRNA Translation: CAG46774.1 CR542003 mRNA Translation: CAG46800.1 EU326309 Genomic DNA Translation: ACA05917.1 AL021155 Genomic DNA No translation available. CH471130 Genomic DNA Translation: EAW71718.1 BC025785 mRNA Translation: AAH25785.1 |
CCDSi | CCDS140.1 |
PIRi | A36736 AWHUB |
RefSeqi | NP_002512.1, NM_002521.2 |
UniGenei | Hs.219140 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1YK1 | X-ray | 2.90 | E | 111-131 | [»] | |
3N56 | X-ray | 3.10 | C/D | 103-134 | [»] | |
DisProti | DP00551 | |||||
ProteinModelPortali | P16860 | |||||
SMRi | P16860 | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Protein-protein interaction databases
BioGridi | 110939, 19 interactors |
IntActi | P16860, 10 interactors |
MINTi | P16860 |
STRINGi | 9606.ENSP00000365651 |
Chemistry databases
DrugBanki | DB01136 Carvedilol DB06412 Oxymetholone |
PTM databases
iPTMneti | P16860 |
PhosphoSitePlusi | P16860 |
Polymorphism and mutation databases
BioMutai | NPPB |
DMDMi | 113836 |
Proteomic databases
PaxDbi | P16860 |
PeptideAtlasi | P16860 |
PRIDEi | P16860 |
ProteomicsDBi | 53393 |
Protocols and materials databases
DNASUi | 4879 |
Structural Biology Knowledgebase | Search... |
Genome annotation databases
Ensembli | ENST00000376468; ENSP00000365651; ENSG00000120937 |
GeneIDi | 4879 |
KEGGi | hsa:4879 |
UCSCi | uc001atj.4 human |
Organism-specific databases
CTDi | 4879 |
DisGeNETi | 4879 |
EuPathDBi | HostDB:ENSG00000120937.8 |
GeneCardsi | NPPB |
HGNCi | HGNC:7940 NPPB |
MIMi | 600295 gene |
neXtProti | NX_P16860 |
OpenTargetsi | ENSG00000120937 |
PharmGKBi | PA31734 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG410J2F8 Eukaryota ENOG410ZE1B LUCA |
GeneTreei | ENSGT00940000154513 |
HOGENOMi | HOG000033937 |
HOVERGENi | HBG078206 |
InParanoidi | P16860 |
KOi | K12335 |
OMAi | GCNVLRR |
OrthoDBi | 1493586at2759 |
PhylomeDBi | P16860 |
TreeFami | TF106304 |
Enzyme and pathway databases
SIGNORi | P16860 |
Miscellaneous databases
ChiTaRSi | NPPB human |
EvolutionaryTracei | P16860 |
GeneWikii | Brain_natriuretic_peptide |
GenomeRNAii | 4879 |
PMAP-CutDBi | P16860 |
PROi | PR:P16860 |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000120937 Expressed in 86 organ(s), highest expression level in right atrium auricular region |
Genevisiblei | P16860 HS |
Family and domain databases
InterProi | View protein in InterPro IPR000663 Natr_peptide IPR030480 Natr_peptide_CS IPR002408 Natriuretic_peptide_brain |
Pfami | View protein in Pfam PF00212 ANP, 1 hit |
PRINTSi | PR00712 BNATPEPTIDE |
SMARTi | View protein in SMART SM00183 NAT_PEP, 1 hit |
PROSITEi | View protein in PROSITE PS00263 NATRIURETIC_PEPTIDE, 1 hit |
ProtoNeti | Search... |
Entry informationi
Entry namei | ANFB_HUMAN | |
Accessioni | P16860Primary (citable) accession number: P16860 Secondary accession number(s): B0ZBE9, Q6FGY0, Q9P2Q7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1990 |
Last sequence update: | August 1, 1990 | |
Last modified: | February 13, 2019 | |
This is version 186 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Complete proteome, Direct protein sequencing, Pharmaceutical, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations - Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations