UniProtKB - P16855 (VNFD_AZOVI)
Protein
Nitrogenase vanadium-iron protein alpha chain
Gene
vnfD
Organism
Azotobacter vinelandii
Status
Functioni
This vanadium-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation.
Miscellaneous
The structure of the 7Fe-V-9S-C-homocitryl cluster is assumed to be analogous to the 7Fe-Mo-9S-C-homocitryl cluster.
Catalytic activityi
- 16 ATP + 16 H2O + N2 + 8 reduced [2Fe-2S]-[ferredoxin] = 16 ADP + 6 H+ + H2 + 2 NH4+ + 8 oxidized [2Fe-2S]-[ferredoxin] + 16 phosphateEC:1.18.6.1
Cofactori
Protein has several cofactor binding sites:- [8Fe-7S] clusterBy similarityNote: Binds 1 [8Fe-7S] cluster per heterodimer.By similarity
- [7Fe-V-9S-C-homocitryl] clusterBy similarityNote: Binds 1 [7Fe-V-9S-C-homocitryl] cluster per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 49 | Iron-sulfur (8Fe-7S); shared with beta chainBy similarity | 1 | |
Metal bindingi | 75 | Iron-sulfur (8Fe-7S); shared with beta chainBy similarity | 1 | |
Metal bindingi | 138 | Iron-sulfur (8Fe-7S); shared with beta chainBy similarity | 1 | |
Metal bindingi | 257 | Vanadium-iron-sulfur-carbon (7Fe-V-9S-C-homocitryl)By similarity | 1 | |
Metal bindingi | 423 | Vanadium-iron-sulfur-carbon (7Fe-V-9S-C-homocitryl); via pros nitrogenBy similarity | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- carbonyl sulfide nitrogenase activity Source: UniProtKB-EC
- iron-sulfur cluster binding Source: UniProtKB-KW
- nitrogenase activity Source: UniProtKB-EC
- vanadium ion binding Source: InterPro
GO - Biological processi
- nitrogen fixation Source: UniProtKB-KW
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Nitrogen fixation |
Ligand | ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding, Vanadium |
Names & Taxonomyi
Protein namesi | Recommended name: Nitrogenase vanadium-iron protein alpha chain (EC:1.18.6.1)Alternative name(s): Dinitrogenase 2 subunit alpha Nitrogenase component I |
Gene namesi | Name:vnfD |
Organismi | Azotobacter vinelandii |
Taxonomic identifieri | 354 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Azotobacter |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed | |||
ChainiPRO_0000153060 | 2 – 474 | Nitrogenase vanadium-iron protein alpha chainAdd BLAST | 473 |
Interactioni
Subunit structurei
Hexamer of two alpha, two beta, and two delta chains.
Protein-protein interaction databases
DIPi | DIP-48893N |
IntActi | P16855, 2 interactors |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P16855 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the NifD/NifK/NifE/NifN family.Curated
Family and domain databases
InterProi | View protein in InterPro IPR000510, Nase/OxRdtase_comp1 IPR005974, Nase_asu IPR010143, Nase_comp1_asu IPR000318, Nase_comp1_CS IPR010142, Nase_V-Fe_asu |
PANTHERi | PTHR43457, PTHR43457, 1 hit |
Pfami | View protein in Pfam PF00148, Oxidored_nitro, 1 hit |
TIGRFAMsi | TIGR01284, alt_nitrog_alph, 1 hit TIGR01862, N2-ase-Ialpha, 1 hit TIGR01860, VNFD, 1 hit |
PROSITEi | View protein in PROSITE PS00699, NITROGENASE_1_1, 1 hit PS00090, NITROGENASE_1_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P16855-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPMVLLECDK DIPERQKHIY LKAPNEDTRE FLPIANAATI PGTLSERGCA
60 70 80 90 100
FCGAKLVIGG VLKDTIQMIH GPLGCAYDTW HTKRYPTDNG HFNMKYVWST
110 120 130 140 150
DMKESHVVFG GEKRLEKSMH EAFDEMPDIK RMIVYTTCPT ALIGDDIKAV
160 170 180 190 200
AKKVMKDRPD VDVFTVECPG FSGVSQSKGH HVLNIGWINE KVETMEKEIT
210 220 230 240 250
SEYTMNFIGD FNIQGDTQLL QTYWDRLGIQ VVAHFTGNGT YDDLRCMHQA
260 270 280 290 300
QLNVVNCARS SGYIANELKK RYGIPRLDID SWGFNYMAEG IRKICAFFGI
310 320 330 340 350
EEKGEELIAE EYAKWKPKLD WYKERLQGKK MAIWTGGPRL WHWTKSVEDD
360 370 380 390 400
LGVQVVAMSS KFGHEEDFEK VIARGKEGTY YIDDGNELEF FEIIDLVKPD
410 420 430 440 450
VIFTGPRVGE LVKKLHIPYV NGHGYHNGPY MGFEGFVNLA RDMYNAVHNP
460 470
LRHLAAVDIR DKSQTTPVIV RGAA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M32371 Genomic DNA Translation: AAA22172.1 |
PIRi | C35405 |
RefSeqi | WP_012698950.1, NZ_FPKM01000002.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M32371 Genomic DNA Translation: AAA22172.1 |
PIRi | C35405 |
RefSeqi | WP_012698950.1, NZ_FPKM01000002.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5N6Y | X-ray | 1.35 | A/D | 1-474 | [»] | |
7ADR | X-ray | 1.00 | A/D | 1-474 | [»] | |
7ADY | X-ray | 1.05 | A/D | 1-474 | [»] | |
SMRi | P16855 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-48893N |
IntActi | P16855, 2 interactors |
Family and domain databases
InterProi | View protein in InterPro IPR000510, Nase/OxRdtase_comp1 IPR005974, Nase_asu IPR010143, Nase_comp1_asu IPR000318, Nase_comp1_CS IPR010142, Nase_V-Fe_asu |
PANTHERi | PTHR43457, PTHR43457, 1 hit |
Pfami | View protein in Pfam PF00148, Oxidored_nitro, 1 hit |
TIGRFAMsi | TIGR01284, alt_nitrog_alph, 1 hit TIGR01862, N2-ase-Ialpha, 1 hit TIGR01860, VNFD, 1 hit |
PROSITEi | View protein in PROSITE PS00699, NITROGENASE_1_1, 1 hit PS00090, NITROGENASE_1_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | VNFD_AZOVI | |
Accessioni | P16855Primary (citable) accession number: P16855 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1990 |
Last sequence update: | January 23, 2007 | |
Last modified: | April 7, 2021 | |
This is version 90 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families