UniProtKB - P16853 (GP_HANTL)
Envelopment polyprotein
GP
Functioni
Forms homotetramers with glycoprotein C at the surface of the virion. Attaches the virion to host cell receptors including integrin beta3/ITGB3. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Also promotes fusion of viral membrane with host endosomal membrane after endocytosis of the virion. May dysregulate normal immune and endothelial cell responses through an ITAM motif.
By similarityForms homotetramers with glycoprotein N at the surface of the virion. Attaches the virion to host cell receptors including integrin beta3/ITGB3. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Also promotes fusion of viral membrane with host endosomal membrane after endocytosis of the virion.
By similarityGO - Biological processi
- endocytosis involved in viral entry into host cell Source: UniProtKB-KW
- fusion of virus membrane with host endosome membrane Source: UniProtKB-KW
- signal transduction Source: InterPro
- suppression by virus of host innate immune response Source: UniProtKB-KW
- suppression by virus of host TRAF activity Source: UniProtKB-KW
- virion attachment to host cell Source: UniProtKB-KW
Keywordsi
Names & Taxonomyi
Protein namesi | Recommended name: Envelopment polyproteinAlternative name(s): M polyprotein Cleaved into the following 2 chains: Alternative name(s): Glycoprotein G1 Alternative name(s): Glycoprotein G2 |
Gene namesi | Name:GP |
Organismi | Hantaan virus (strain Lee) (Lee virus) (Korean hemorrhagic fever virus) |
Taxonomic identifieri | 11601 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Negarnaviricota › Polyploviricotina › Ellioviricetes › Bunyavirales › Hantaviridae › Mammantavirinae › Orthohantavirus › |
Virus hosti | Apodemus agrarius (Eurasian field mouse) [TaxID: 39030] Homo sapiens (Human) [TaxID: 9606] |
Subcellular locationi
- Virion membrane By similarity
- Host cell surface By similarity
- Host Golgi apparatus membrane By similarity; Single-pass type I membrane protein By similarity
- Host endoplasmic reticulum membrane By similarity; Single-pass type I membrane protein By similarity Note: Interaction between glycoprotein N and glycoprotein C is essential for proper targeting of glycoprotein N to the host Golgi complex, where virion budding occurs.By similarity
- Virion membrane By similarity
- Host cell surface By similarity
- Host Golgi apparatus membrane By similarity; Single-pass type I membrane protein By similarity
- Host endoplasmic reticulum membrane By similarity; Single-pass type I membrane protein By similarity
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 19 – 485 | LumenalSequence analysisAdd BLAST | 467 | |
Transmembranei | 486 – 506 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 507 – 648 | CytoplasmicSequence analysisAdd BLAST | 142 | |
Topological domaini | 649 – 1105 | LumenalSequence analysisAdd BLAST | 457 | |
Transmembranei | 1106 – 1126 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1127 – 1135 | CytoplasmicSequence analysis | 9 |
Keywords - Cellular componenti
Host endoplasmic reticulum, Host Golgi apparatus, Host membrane, Membrane, Viral envelope protein, VirionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 18 | Sequence analysisAdd BLAST | 18 | |
ChainiPRO_0000036812 | 19 – 1135 | Envelopment polyproteinAdd BLAST | 1117 | |
ChainiPRO_0000036813 | 19 – 648 | Glycoprotein NBy similarityAdd BLAST | 630 | |
ChainiPRO_0000036814 | 649 – 1135 | Glycoprotein CBy similarityAdd BLAST | 487 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 134 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 235 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 347 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 399 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Disulfide bondi | 735 ↔ 770 | By similarity | ||
Disulfide bondi | 739 ↔ 777 | By similarity | ||
Disulfide bondi | 751 ↔ 885 | By similarity | ||
Disulfide bondi | 765 ↔ 896 | By similarity | ||
Disulfide bondi | 780 ↔ 904 | By similarity | ||
Disulfide bondi | 806 ↔ 815 | By similarity | ||
Disulfide bondi | 823 ↔ 832 | By similarity | ||
Disulfide bondi | 863 ↔ 867 | By similarity | ||
Glycosylationi | 928 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Disulfide bondi | 970 ↔ 1000 | By similarity | ||
Disulfide bondi | 993 ↔ 1045 | By similarity | ||
Disulfide bondi | 1010 ↔ 1015 | By similarity | ||
Disulfide bondi | 1046 ↔ 1051 | By similarity |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 648 – 649 | Cleavage; by host signal peptidaseBy similarity | 2 |
Keywords - PTMi
Disulfide bond, GlycoproteinInteractioni
Subunit structurei
Forms homotetramers with glycoprotein C to generate Gn-Gc spikes found on viral surface.
By similarityForms homotetramers with glycoprotein N to generate Gn-Gc spikes found on viral surface.
Interacts with host integrin beta3/ITGB3.
By similarityFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 611 – 634 | ITAMPROSITE-ProRule annotationAdd BLAST | 24 |
Sequence similaritiesi
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixFamily and domain databases
InterProi | View protein in InterPro IPR016402, Envelope_glycoprot_Hantavirus IPR002534, Hanta_G1 IPR002532, Hanta_G2 IPR012316, ITAM_motif_hantavir-typ |
Pfami | View protein in Pfam PF01567, Hanta_G1, 1 hit PF01561, Hanta_G2, 1 hit PF10538, ITAM_Cys-rich, 1 hit |
PIRSFi | PIRSF003945, M_poly_HantaV, 1 hit |
PROSITEi | View protein in PROSITE PS51056, ITAM_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MGIWKWLVMA SLVWPVLTLR NVYDMKIECP HTVSFGENSV IGYVELPPMP
60 70 80 90 100
LADTAQLVPE SSCSMDNHQS LNTITKYTQV SWRGKADQSQ SSQTSFETVS
110 120 130 140 150
TEVDLKGTCV LKHKMVEESY RSRKSITCYD LSCNSTYCKP TLYMIVPIHA
160 170 180 190 200
CNMMKSCLIA LGPYRVQVVY ERTYCMTGVL IEGKCFVPDQ SVVSIIKHGI
210 220 230 240 250
FDIASVHIVC FFVAVKGNTY KIFEQVKKSF ESTCNDTENK VQGYYICIVG
260 270 280 290 300
GNSAPIYVPT LDDFRSMEAF TGIFRSPHGE DHDLAGEETA TYSIVGPANA
310 320 330 340 350
KVPHSASSDT LSLIAFSGIP SDSSLSILTS STEAKHVFSP GLFPKLNHTN
360 370 380 390 400
CDKGAIPLMW TGMIDLPGYY EAIHPCTVFC VLSGPGASCE AFSEGGIFNI
410 420 430 440 450
TYPMCLVSKQ NRFRLTEQQV NFVCQRVDVD IVVYCNGQRK VILTKTLVIG
460 470 480 490 500
QCIYTITSLF SLLPGVAHSI AVELCVPGFH GWATAALLVT FCFGWVLIPA
510 520 530 540 550
ITFIILTILK FIANIFHTSN QENRLKSVLR KIKEEFEKTK GSMVCDVCKY
560 570 580 590 600
ECETYKELKA HGVSCPQSQC PYCFTHCEPT EAAFQAHYKV CQVTHRFRDD
610 620 630 640 650
LKKTVTPQNF TPGCYRTLNL FRYKSRCYIF TMWIFLLVLE SILWAASASE
660 670 680 690 700
TPLTPVWNDN AHGVGSVPMH TDLELDFSLT SSSKYTYRRK LTNPLEEAQS
710 720 730 740 750
IDLHIEIEEQ TIGVDVHALG HWFDGRLNLK TSFHCYGACT KYEYPWHTAK
760 770 780 790 800
CHYERDYQYE TSWGCNPSDC PGVGTGCTAC GLYLDRLKPV GSAYKIITIR
810 820 830 840 850
YSRRVCVQFG EENLCKIIDM NDCFVSRHVK VCIIGTVSKF SQGDTLLFFG
860 870 880 890 900
PLEGGGLIFK HWCTSTCQFG DPGDIMSPRD KGFLCPEFPG SFRKKCNFAT
910 920 930 940 950
TPICEYDGNM VSGYKKVMAT IDSFQSFNTS TMHFTDERIE WKDPDGMLRD
960 970 980 990 1000
HINILVTKDI DFDNLGENPC KIGLQTSSIE GAWGSGVGFT LTCLVSLTEC
1010 1020 1030 1040 1050
PTFLTSIKAC DKAICYGAES VTLTRGQNTV KVSGKGGHSG STFKCCHGED
1060 1070 1080 1090 1100
CSQIGLHAAA PHLDKVNGIS EMENSKEYDD GAPQCGIKCW FVKSGEWISG
1110 1120 1130
IFSGNWIVLI VLCVFLLFSL VLLSILCPVR KHKKS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D00377 Genomic RNA Translation: BAA00280.1 |
PIRi | JS0605 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D00377 Genomic RNA Translation: BAA00280.1 |
PIRi | JS0605 |
3D structure databases
SMRi | P16853 |
ModBasei | Search... |
Family and domain databases
InterProi | View protein in InterPro IPR016402, Envelope_glycoprot_Hantavirus IPR002534, Hanta_G1 IPR002532, Hanta_G2 IPR012316, ITAM_motif_hantavir-typ |
Pfami | View protein in Pfam PF01567, Hanta_G1, 1 hit PF01561, Hanta_G2, 1 hit PF10538, ITAM_Cys-rich, 1 hit |
PIRSFi | PIRSF003945, M_poly_HantaV, 1 hit |
PROSITEi | View protein in PROSITE PS51056, ITAM_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GP_HANTL | |
Accessioni | P16853Primary (citable) accession number: P16853 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1990 |
Last sequence update: | August 1, 1990 | |
Last modified: | December 2, 2020 | |
This is version 100 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |