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Protein

Cysteine synthase B

Gene

cysM

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Two cysteine synthase enzymes are found. Both catalyze the same reaction. Cysteine synthase B can also use thiosulfate in place of sulfide to give cysteine thiosulfonate as a product.

Catalytic activityi

O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate.

Cofactori

Pathwayi: L-cysteine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-cysteine from L-serine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Serine acetyltransferase (cysE)
  2. Cysteine synthase B (cysM), Cysteine synthase A (cysK)
This subpathway is part of the pathway L-cysteine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-cysteine from L-serine, the pathway L-cysteine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei71Pyridoxal phosphate1 Publication1
Binding sitei255Pyridoxal phosphate1 Publication1

GO - Molecular functioni

  • cysteine synthase activity Source: EcoCyc
  • L-cysteine desulfhydrase activity Source: EcoCyc
  • pyridoxal phosphate binding Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processAmino-acid biosynthesis, Cysteine biosynthesis
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciEcoCyc:ACSERLYB-MONOMER
MetaCyc:ACSERLYB-MONOMER
BRENDAi2.5.1.47 2026
SABIO-RKiP16703
UniPathwayi
UPA00136;UER00200

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine synthase B (EC:2.5.1.47)
Short name:
CSase B
Alternative name(s):
O-acetylserine (thiol)-lyase B
Short name:
OAS-TL B
O-acetylserine sulfhydrylase B
Gene namesi
Name:cysM
Ordered Locus Names:b2421, JW2414
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10193 cysM

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001671081 – 303Cysteine synthase BAdd BLAST303

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei41N6-(pyridoxal phosphate)lysine1

Proteomic databases

PaxDbiP16703
PRIDEiP16703

2D gel databases

SWISS-2DPAGEiP16703

Interactioni

Subunit structurei

Homodimer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
accCP241823EBI-542376,EBI-542308

Protein-protein interaction databases

BioGridi4259326, 6 interactors
DIPiDIP-9383N
IntActiP16703, 21 interactors
STRINGi316385.ECDH10B_2586

Structurei

Secondary structure

1303
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP16703
SMRiP16703
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP16703

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni174 – 178Pyridoxal phosphate binding5

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C6T Bacteria
COG0031 LUCA
HOGENOMiHOG000217394
InParanoidiP16703
KOiK12339
PhylomeDBiP16703

Family and domain databases

InterProiView protein in InterPro
IPR005856 Cys_synth
IPR005858 CysM
IPR001216 P-phosphate_BS
IPR001926 PLP-dep
IPR036052 Trypto_synt_PLP_dependent
PANTHERiPTHR10314:SF100 PTHR10314:SF100, 1 hit
PfamiView protein in Pfam
PF00291 PALP, 1 hit
SUPFAMiSSF53686 SSF53686, 1 hit
TIGRFAMsiTIGR01136 cysKM, 1 hit
TIGR01138 cysM, 1 hit
PROSITEiView protein in PROSITE
PS00901 CYS_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

P16703-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSTLEQTIGN TPLVKLQRMG PDNGSEVWLK LEGNNPAGSV KDRAALSMIV
60 70 80 90 100
EAEKRGEIKP GDVLIEATSG NTGIALAMIA ALKGYRMKLL MPDNMSQERR
110 120 130 140 150
AAMRAYGAEL ILVTKEQGME GARDLALEMA NRGEGKLLDQ FNNPDNPYAH
160 170 180 190 200
YTTTGPEIWQ QTGGRITHFV SSMGTTGTIT GVSRFMREQS KPVTIVGLQP
210 220 230 240 250
EEGSSIPGIR RWPTEYLPGI FNASLVDEVL DIHQRDAENT MRELAVREGI
260 270 280 290 300
FCGVSSGGAV AGALRVAKAN PDAVVVAIIC DRGDRYLSTG VFGEEHFSQG

AGI
Length:303
Mass (Da):32,664
Last modified:August 1, 1990 - v1
Checksum:i1C7FBCB943FCFE39
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32101 Genomic DNA Translation: AAA23640.1
U00096 Genomic DNA Translation: AAC75474.1
AP009048 Genomic DNA Translation: BAA16295.1
PIRiD35402 SYECBC
RefSeqiNP_416916.1, NC_000913.3
WP_001336044.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75474; AAC75474; b2421
BAA16295; BAA16295; BAA16295
GeneIDi946888
KEGGiecj:JW2414
eco:b2421
PATRICifig|1411691.4.peg.4310

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32101 Genomic DNA Translation: AAA23640.1
U00096 Genomic DNA Translation: AAC75474.1
AP009048 Genomic DNA Translation: BAA16295.1
PIRiD35402 SYECBC
RefSeqiNP_416916.1, NC_000913.3
WP_001336044.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BHSX-ray2.67A/B/C/D1-303[»]
2BHTX-ray2.10A/B/C/D1-303[»]
2V03X-ray1.33A1-303[»]
ProteinModelPortaliP16703
SMRiP16703
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259326, 6 interactors
DIPiDIP-9383N
IntActiP16703, 21 interactors
STRINGi316385.ECDH10B_2586

2D gel databases

SWISS-2DPAGEiP16703

Proteomic databases

PaxDbiP16703
PRIDEiP16703

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75474; AAC75474; b2421
BAA16295; BAA16295; BAA16295
GeneIDi946888
KEGGiecj:JW2414
eco:b2421
PATRICifig|1411691.4.peg.4310

Organism-specific databases

EchoBASEiEB0190
EcoGeneiEG10193 cysM

Phylogenomic databases

eggNOGiENOG4105C6T Bacteria
COG0031 LUCA
HOGENOMiHOG000217394
InParanoidiP16703
KOiK12339
PhylomeDBiP16703

Enzyme and pathway databases

UniPathwayi
UPA00136;UER00200

BioCyciEcoCyc:ACSERLYB-MONOMER
MetaCyc:ACSERLYB-MONOMER
BRENDAi2.5.1.47 2026
SABIO-RKiP16703

Miscellaneous databases

EvolutionaryTraceiP16703
PROiPR:P16703

Family and domain databases

InterProiView protein in InterPro
IPR005856 Cys_synth
IPR005858 CysM
IPR001216 P-phosphate_BS
IPR001926 PLP-dep
IPR036052 Trypto_synt_PLP_dependent
PANTHERiPTHR10314:SF100 PTHR10314:SF100, 1 hit
PfamiView protein in Pfam
PF00291 PALP, 1 hit
SUPFAMiSSF53686 SSF53686, 1 hit
TIGRFAMsiTIGR01136 cysKM, 1 hit
TIGR01138 cysM, 1 hit
PROSITEiView protein in PROSITE
PS00901 CYS_SYNTHASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCYSM_ECOLI
AccessioniPrimary (citable) accession number: P16703
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: November 7, 2018
This is version 164 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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