UniProtKB - P16701 (CYST_ECOLI)
Protein
Sulfate transport system permease protein CysT
Gene
cysU
Organism
Escherichia coli (strain K12)
Status
Functioni
Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane.
GO - Molecular functioni
- ATPase-coupled sulfate transmembrane transporter activity Source: InterPro
GO - Biological processi
- sulfate transport Source: EcoCyc
Keywordsi
Biological process | Sulfate transport, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:CYST-MONOMER MetaCyc:CYST-MONOMER |
Protein family/group databases
TCDBi | 3.A.1.6.1, the atp-binding cassette (abc) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: Sulfate transport system permease protein CysT |
Gene namesi | Name:cysU Synonyms:cysT Ordered Locus Names:b2424, JW2417 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane 1 Publication; Multi-pass membrane protein PROSITE-ProRule annotation1 Publication
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 17 – 37 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 64 – 84 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 99 – 119 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 136 – 156 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 185 – 205 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 215 – 235 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 243 – 263 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 |
GO - Cellular componenti
- integral component of membrane Source: UniProtKB-KW
- plasma membrane Source: EcoCyc
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000059989 | 1 – 277 | Sulfate transport system permease protein CysTAdd BLAST | 277 |
Proteomic databases
PaxDbi | P16701 |
PRIDEi | P16701 |
Interactioni
Subunit structurei
The complex is composed of two ATP-binding proteins (CysA), two transmembrane proteins (CysT and CysW) and a solute-binding protein (CysP).
CuratedProtein-protein interaction databases
BioGRIDi | 4263208, 7 interactors |
ComplexPortali | CPX-4385, Sulfate/thiosulfate ABC transporter complex, cypP variant CPX-4386, Sulfate/thiosulfate ABC transporter complex, sbp variant |
STRINGi | 511145.b2424 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 60 – 263 | ABC transmembrane type-1PROSITE-ProRule annotationAdd BLAST | 204 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0555, Bacteria |
HOGENOMi | CLU_016047_14_0_6 |
InParanoidi | P16701 |
PhylomeDBi | P16701 |
Family and domain databases
CDDi | cd06261, TM_PBP2, 1 hit |
Gene3Di | 1.10.3720.10, 1 hit |
InterProi | View protein in InterPro IPR011865, CysT_permease IPR000515, MetI-like IPR035906, MetI-like_sf IPR005667, Sulph_transpt2 |
PANTHERi | PTHR30406, PTHR30406, 1 hit PTHR30406:SF10, PTHR30406:SF10, 1 hit |
Pfami | View protein in Pfam PF00528, BPD_transp_1, 1 hit |
SUPFAMi | SSF161098, SSF161098, 1 hit |
TIGRFAMsi | TIGR00969, 3a0106s02, 1 hit TIGR02139, permease_CysT, 1 hit |
PROSITEi | View protein in PROSITE PS50928, ABC_TM1, 1 hit |
i Sequence
Sequence statusi: Complete.
P16701-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFAVSSRRVL PGFTLSLGTS LLFVCLILLL PLSALVMQLA QMSWAQYWEV
60 70 80 90 100
ITNPQVVAAY KVTLLSAFVA SIFNGVFGLL MAWILTRYRF PGRTLLDALM
110 120 130 140 150
DLPFALPTAV AGLTLASLFS VNGFYGEWLA KFDIKVTYTW LGIAVAMAFT
160 170 180 190 200
SIPFVVRTVQ PVLEELGPEY EEAAETLGAT RWQSFCKVVL PELSPALVAG
210 220 230 240 250
VALSFTRSLG EFGAVIFIAG NIAWKTEVTS LMIFVRLQEF DYPAASAIAS
260 270
VILAASLLLL FSINTLQSRF GRRVVGH
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 127 | E → F (PubMed:2188959).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M32101 Genomic DNA Translation: AAA23637.1 U00096 Genomic DNA Translation: AAC75477.1 AP009048 Genomic DNA Translation: BAA16298.1 |
PIRi | A35402, QRECST |
RefSeqi | NP_416919.1, NC_000913.3 WP_000458406.1, NZ_SSUR01000044.1 |
Genome annotation databases
EnsemblBacteriai | AAC75477; AAC75477; b2424 BAA16298; BAA16298; BAA16298 |
GeneIDi | 48134749 946882 |
KEGGi | ecj:JW2417 eco:b2424 |
PATRICi | fig|1411691.4.peg.4307 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M32101 Genomic DNA Translation: AAA23637.1 U00096 Genomic DNA Translation: AAC75477.1 AP009048 Genomic DNA Translation: BAA16298.1 |
PIRi | A35402, QRECST |
RefSeqi | NP_416919.1, NC_000913.3 WP_000458406.1, NZ_SSUR01000044.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 4263208, 7 interactors |
ComplexPortali | CPX-4385, Sulfate/thiosulfate ABC transporter complex, cypP variant CPX-4386, Sulfate/thiosulfate ABC transporter complex, sbp variant |
STRINGi | 511145.b2424 |
Protein family/group databases
TCDBi | 3.A.1.6.1, the atp-binding cassette (abc) superfamily |
Proteomic databases
PaxDbi | P16701 |
PRIDEi | P16701 |
Genome annotation databases
EnsemblBacteriai | AAC75477; AAC75477; b2424 BAA16298; BAA16298; BAA16298 |
GeneIDi | 48134749 946882 |
KEGGi | ecj:JW2417 eco:b2424 |
PATRICi | fig|1411691.4.peg.4307 |
Organism-specific databases
EchoBASEi | EB0194 |
Phylogenomic databases
eggNOGi | COG0555, Bacteria |
HOGENOMi | CLU_016047_14_0_6 |
InParanoidi | P16701 |
PhylomeDBi | P16701 |
Enzyme and pathway databases
BioCyci | EcoCyc:CYST-MONOMER MetaCyc:CYST-MONOMER |
Miscellaneous databases
PROi | PR:P16701 |
Family and domain databases
CDDi | cd06261, TM_PBP2, 1 hit |
Gene3Di | 1.10.3720.10, 1 hit |
InterProi | View protein in InterPro IPR011865, CysT_permease IPR000515, MetI-like IPR035906, MetI-like_sf IPR005667, Sulph_transpt2 |
PANTHERi | PTHR30406, PTHR30406, 1 hit PTHR30406:SF10, PTHR30406:SF10, 1 hit |
Pfami | View protein in Pfam PF00528, BPD_transp_1, 1 hit |
SUPFAMi | SSF161098, SSF161098, 1 hit |
TIGRFAMsi | TIGR00969, 3a0106s02, 1 hit TIGR02139, permease_CysT, 1 hit |
PROSITEi | View protein in PROSITE PS50928, ABC_TM1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CYST_ECOLI | |
Accessioni | P16701Primary (citable) accession number: P16701 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1990 |
Last sequence update: | August 1, 1990 | |
Last modified: | December 2, 2020 | |
This is version 161 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families