UniProtKB - P16692 (PHNP_ECOLI)
Protein
Phosphoribosyl 1,2-cyclic phosphate phosphodiesterase
Gene
phnP
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the hydrolysis of the cyclic ribose-phosphate to form alpha-D-ribose 1,5-bisphosphate.2 Publications
Miscellaneous
The sequence shown is that of strains K12 and B.
Catalytic activityi
- EC:3.1.4.551 Publication
Cofactori
Protein has several cofactor binding sites:- Mn2+1 PublicationNote: Binds 2 manganese ions per subunit.1 Publication
- Zn2+1 PublicationNote: Binds 1 zinc ion per subunit.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 21 | Zinc2 Publications | 1 | |
Metal bindingi | 23 | Zinc2 Publications | 1 | |
Metal bindingi | 26 | Zinc2 Publications | 1 | |
Metal bindingi | 76 | Manganese 1; via tele nitrogen2 Publications | 1 | |
Metal bindingi | 78 | Manganese 1; via pros nitrogen2 Publications | 1 | |
Metal bindingi | 80 | Manganese 22 Publications | 1 | |
Metal bindingi | 81 | Manganese 2; via tele nitrogen2 Publications | 1 | |
Metal bindingi | 143 | Manganese 1; via tele nitrogen2 Publications | 1 | |
Metal bindingi | 164 | Manganese 12 Publications | 1 | |
Metal bindingi | 164 | Manganese 22 Publications | 1 | |
Metal bindingi | 222 | Manganese 2; via tele nitrogen2 Publications | 1 | |
Metal bindingi | 225 | Zinc; via pros nitrogen2 Publications | 1 |
GO - Molecular functioni
- 5-phospho-alpha-D-ribosyl 1,2-cyclic phosphate phosphodiesterase activity Source: EcoCyc
- manganese ion binding Source: EcoCyc
GO - Biological processi
- organic phosphonate catabolic process Source: EcoCyc
Keywordsi
Molecular function | Hydrolase |
Ligand | Manganese, Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10725-MONOMER MetaCyc:EG10725-MONOMER |
BRENDAi | 3.1.4.55, 2026 |
Names & Taxonomyi
Protein namesi | Recommended name: Phosphoribosyl 1,2-cyclic phosphate phosphodiesterase (EC:3.1.4.55)Alternative name(s): Phosphoribosyl cyclic phosphodiesterase |
Gene namesi | Name:phnP Ordered Locus Names:b4092, JW4053 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 54 | D → A: Has a 1000-fold lower activity. 1 Publication | 1 | |
Mutagenesisi | 75 | T → A: Has 40-fold lower activity. 1 Publication | 1 | |
Mutagenesisi | 78 | H → A: Has 250-fold lower activity. 1 Publication | 1 | |
Mutagenesisi | 80 | D → A: Has 1000-fold lower activity, and has 100-fold lower affinity for manganese. 1 Publication | 1 | |
Mutagenesisi | 164 | D → A: Has a 1000-fold lower activity. 1 Publication | 1 | |
Mutagenesisi | 187 | D → A: No effect. 1 Publication | 1 | |
Mutagenesisi | 200 | H → A: Has 10-fold lower activity. 1 Publication | 1 | |
Mutagenesisi | 222 | H → A: Has 9-fold lower activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000058402 | 1 – 252 | Phosphoribosyl 1,2-cyclic phosphate phosphodiesteraseAdd BLAST | 252 |
Proteomic databases
jPOSTi | P16692 |
PaxDbi | P16692 |
PRIDEi | P16692 |
Interactioni
Subunit structurei
Homodimer.
2 PublicationsProtein-protein interaction databases
BioGRIDi | 4261542, 14 interactors |
IntActi | P16692, 1 interactor |
STRINGi | 511145.b4092 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P16692 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P16692 |
Family & Domainsi
Phylogenomic databases
eggNOGi | COG1235, Bacteria |
HOGENOMi | CLU_044538_3_0_6 |
InParanoidi | P16692 |
PhylomeDBi | P16692 |
Family and domain databases
CDDi | cd07736, PhnP-like_MBL-fold, 1 hit |
Gene3Di | 3.60.15.10, 1 hit |
InterProi | View protein in InterPro IPR001279, Metallo-B-lactamas IPR035682, PhnP_MBL IPR017693, Phosphonate_metab_PhnP IPR036866, RibonucZ/Hydroxyglut_hydro |
Pfami | View protein in Pfam PF12706, Lactamase_B_2, 1 hit |
SUPFAMi | SSF56281, SSF56281, 1 hit |
TIGRFAMsi | TIGR03307, PhnP, 1 hit |
i Sequence
Sequence statusi: Complete.
P16692-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSLTLTLTGT GGAQGVPAWG CECAACARAR RSPQYRRQPC SGVVKFNDAI
60 70 80 90 100
TLIDAGLHDL ADRWSPGSFQ QFLLTHYHMD HVQGLFPLRW GVGDPIPVYG
110 120 130 140 150
PPDEQGCDDL FKHPGLLDFS HTVEPFVVFD LQGLQVTPLP LNHSKLTFGY
160 170 180 190 200
LLETAHSRVA WLSDTAGLPE KTLKFLRNNQ PQVMVMDCSH PPRADAPRNH
210 220 230 240 250
CDLNTVLALN QVIRSPRVIL THISHQFDAW LMENALPSGF EVGFDGMEIG
VA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J05260 Genomic DNA Translation: AAA24356.1 D90227 Genomic DNA Translation: BAA14276.1 U14003 Genomic DNA Translation: AAA96991.1 U00096 Genomic DNA Translation: AAC77053.1 AP009048 Genomic DNA Translation: BAE78095.1 |
PIRi | H35719 |
RefSeqi | NP_418516.1, NC_000913.3 WP_000059908.1, NZ_SSUW01000018.1 |
Genome annotation databases
EnsemblBacteriai | AAC77053; AAC77053; b4092 BAE78095; BAE78095; BAE78095 |
GeneIDi | 948600 |
KEGGi | ecj:JW4053 eco:b4092 |
PATRICi | fig|1411691.4.peg.2608 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J05260 Genomic DNA Translation: AAA24356.1 D90227 Genomic DNA Translation: BAA14276.1 U14003 Genomic DNA Translation: AAA96991.1 U00096 Genomic DNA Translation: AAC77053.1 AP009048 Genomic DNA Translation: BAE78095.1 |
PIRi | H35719 |
RefSeqi | NP_418516.1, NC_000913.3 WP_000059908.1, NZ_SSUW01000018.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3G1P | X-ray | 1.40 | A/B | 1-252 | [»] | |
3P2U | X-ray | 1.48 | A/B | 1-252 | [»] | |
SMRi | P16692 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261542, 14 interactors |
IntActi | P16692, 1 interactor |
STRINGi | 511145.b4092 |
Proteomic databases
jPOSTi | P16692 |
PaxDbi | P16692 |
PRIDEi | P16692 |
Genome annotation databases
EnsemblBacteriai | AAC77053; AAC77053; b4092 BAE78095; BAE78095; BAE78095 |
GeneIDi | 948600 |
KEGGi | ecj:JW4053 eco:b4092 |
PATRICi | fig|1411691.4.peg.2608 |
Organism-specific databases
EchoBASEi | EB0719 |
Phylogenomic databases
eggNOGi | COG1235, Bacteria |
HOGENOMi | CLU_044538_3_0_6 |
InParanoidi | P16692 |
PhylomeDBi | P16692 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10725-MONOMER MetaCyc:EG10725-MONOMER |
BRENDAi | 3.1.4.55, 2026 |
Miscellaneous databases
EvolutionaryTracei | P16692 |
PROi | PR:P16692 |
Family and domain databases
CDDi | cd07736, PhnP-like_MBL-fold, 1 hit |
Gene3Di | 3.60.15.10, 1 hit |
InterProi | View protein in InterPro IPR001279, Metallo-B-lactamas IPR035682, PhnP_MBL IPR017693, Phosphonate_metab_PhnP IPR036866, RibonucZ/Hydroxyglut_hydro |
Pfami | View protein in Pfam PF12706, Lactamase_B_2, 1 hit |
SUPFAMi | SSF56281, SSF56281, 1 hit |
TIGRFAMsi | TIGR03307, PhnP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PHNP_ECOLI | |
Accessioni | P16692Primary (citable) accession number: P16692 Secondary accession number(s): Q2M6L1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1990 |
Last sequence update: | August 1, 1990 | |
Last modified: | December 2, 2020 | |
This is version 138 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references