UniProtKB - P16691 (PHNO_ECOLI)
Protein
Aminoalkylphosphonate N-acetyltransferase
Gene
phnO
Organism
Escherichia coli (strain K12)
Status
Functioni
Aminoalkylphosphonate N-acetyltransferase which is able to acetylate a range of aminoalkylphosphonic acids, including aminomethylphosphonate, (S)-1-aminoethylphosphonate and 2-aminoethyl- and 3-aminopropylphosphonate, using acetyl-CoA as acetyl donor. Is required for the utilization of aminomethylphosphonate and (S)-1-aminoethylphosphonate as a phosphate source via the C-P lyase pathway. Is also essential for the detoxification of (S)-1-aminoethylphosphonate, a structural analog of D-alanine that has bacteriocidal properties due to inhibition of cell wall synthesis (PubMed:23056305). Also acts as a N-epsilon-lysine acetyltransferase that catalyzes acetylation of several proteins (PubMed:30352934).2 Publications
Miscellaneous
The sequence shown is that of strains K12 and B.Curated
Catalytic activityi
- EC:2.3.1.2801 Publication
Cofactori
a divalent metal cationBy similarity
GO - Molecular functioni
- N-acetyltransferase activity Source: EcoCyc
- peptide-lysine-N-acetyltransferase activity Source: EcoCyc
GO - Biological processi
- aminophosphonate metabolic process Source: EcoCyc
- internal peptidyl-lysine acetylation Source: EcoCyc
Keywordsi
Molecular function | Acyltransferase, Transferase |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10724-MONOMER MetaCyc:EG10724-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:phnO1 Publication Ordered Locus Names:b4093, JW4054 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Cells lacking this gene have lost the ability to utilize aminomethylphosphonate or (S)-1-aminoethylphosphonate as the sole source of phosphate for growth, but are still able to utilize the other aminoalkylphosphonates 2-aminoethyl- and 3-aminopropylphosphonate, and alkylphosphonates. They are also much more susceptible to the bacteriocidal effect of (S)-1-aminoethylphosphonate, even with phosphate or D-alanine present.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000058401 | 1 – 144 | Aminoalkylphosphonate N-acetyltransferaseAdd BLAST | 144 |
Proteomic databases
jPOSTi | P16691 |
PaxDbi | P16691 |
PRIDEi | P16691 |
Interactioni
Subunit structurei
Homodimer.
By similarityProtein-protein interaction databases
BioGRIDi | 4262023, 11 interactors 852893, 1 interactor |
IntActi | P16691, 1 interactor |
STRINGi | 511145.b4093 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 4 – 144 | N-acetyltransferasePROSITE-ProRule annotationAdd BLAST | 141 |
Phylogenomic databases
eggNOGi | COG0456, Bacteria |
HOGENOMi | CLU_013985_34_6_6 |
InParanoidi | P16691 |
PhylomeDBi | P16691 |
Family and domain databases
InterProi | View protein in InterPro IPR016181, Acyl_CoA_acyltransferase IPR000182, GNAT_dom |
Pfami | View protein in Pfam PF00583, Acetyltransf_1, 1 hit |
SUPFAMi | SSF55729, SSF55729, 1 hit |
PROSITEi | View protein in PROSITE PS51186, GNAT, 1 hit |
i Sequence
Sequence statusi: Complete.
P16691-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPACELRPAT QYDTDAVYAL ICELKQAEFD HHAFRVGFNA NLRDPNMRYH
60 70 80 90 100
LALLDGEVVG MIGLHLQFHL HHVNWIGEIQ ELVVMPQARG LNVGSKLLAW
110 120 130 140
AEEEARQAGA EMTELSTNVK RHDAHRFYLR EGYEQSHFRF TKAL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J05260 Genomic DNA Translation: AAA24354.1 D90227 Genomic DNA Translation: BAA14275.1 U14003 Genomic DNA Translation: AAA96992.1 U00096 Genomic DNA Translation: AAC77054.1 AP009048 Genomic DNA Translation: BAE78096.1 |
PIRi | G35719 |
RefSeqi | NP_418517.1, NC_000913.3 WP_001110490.1, NZ_STEB01000014.1 |
Genome annotation databases
EnsemblBacteriai | AAC77054; AAC77054; b4093 BAE78096; BAE78096; BAE78096 |
GeneIDi | 948599 |
KEGGi | ecj:JW4054 eco:b4093 |
PATRICi | fig|1411691.4.peg.2607 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J05260 Genomic DNA Translation: AAA24354.1 D90227 Genomic DNA Translation: BAA14275.1 U14003 Genomic DNA Translation: AAA96992.1 U00096 Genomic DNA Translation: AAC77054.1 AP009048 Genomic DNA Translation: BAE78096.1 |
PIRi | G35719 |
RefSeqi | NP_418517.1, NC_000913.3 WP_001110490.1, NZ_STEB01000014.1 |
3D structure databases
SMRi | P16691 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4262023, 11 interactors 852893, 1 interactor |
IntActi | P16691, 1 interactor |
STRINGi | 511145.b4093 |
Proteomic databases
jPOSTi | P16691 |
PaxDbi | P16691 |
PRIDEi | P16691 |
Genome annotation databases
EnsemblBacteriai | AAC77054; AAC77054; b4093 BAE78096; BAE78096; BAE78096 |
GeneIDi | 948599 |
KEGGi | ecj:JW4054 eco:b4093 |
PATRICi | fig|1411691.4.peg.2607 |
Organism-specific databases
EchoBASEi | EB0718 |
Phylogenomic databases
eggNOGi | COG0456, Bacteria |
HOGENOMi | CLU_013985_34_6_6 |
InParanoidi | P16691 |
PhylomeDBi | P16691 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10724-MONOMER MetaCyc:EG10724-MONOMER |
Miscellaneous databases
PROi | PR:P16691 |
Family and domain databases
InterProi | View protein in InterPro IPR016181, Acyl_CoA_acyltransferase IPR000182, GNAT_dom |
Pfami | View protein in Pfam PF00583, Acetyltransf_1, 1 hit |
SUPFAMi | SSF55729, SSF55729, 1 hit |
PROSITEi | View protein in PROSITE PS51186, GNAT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PHNO_ECOLI | |
Accessioni | P16691Primary (citable) accession number: P16691 Secondary accession number(s): Q2M6L0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1990 |
Last sequence update: | August 1, 1990 | |
Last modified: | December 2, 2020 | |
This is version 143 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |