UniProtKB - P16676 (CYSA_ECOLI)
Protein
Sulfate/thiosulfate import ATP-binding protein CysA
Gene
cysA
Organism
Escherichia coli (strain K12)
Status
Functioni
Part of the ABC transporter complex CysAWTP involved in sulfate/thiosulfate import. Responsible for energy coupling to the transport system.
Miscellaneous
CysPTWAM system can also transport molybdate.
Catalytic activityi
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 35 – 42 | ATPUniRule annotation | 8 |
GO - Molecular functioni
- ABC-type sulfate transporter activity Source: UniProtKB-UniRule
- ABC-type thiosulfate transporter activity Source: UniProtKB-EC
- ATPase activity Source: InterPro
- ATP binding Source: EcoCyc
GO - Biological processi
- sulfate transport Source: GO_Central
Keywordsi
Molecular function | Translocase |
Biological process | Sulfate transport, Transport |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:CYSA-MONOMER MetaCyc:CYSA-MONOMER |
Protein family/group databases
TCDBi | 3.A.1.6.1, the atp-binding cassette (abc) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: Sulfate/thiosulfate import ATP-binding protein CysAUniRule annotation (EC:7.3.2.3UniRule annotation)Alternative name(s): Sulfate-transporting ATPaseUniRule annotation |
Gene namesi | Name:cysAUniRule annotation Ordered Locus Names:b2422, JW2415 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane UniRule annotation; Peripheral membrane protein UniRule annotation
Plasma Membrane
- ATP-binding cassette (ABC) transporter complex Source: InterPro
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000092265 | 1 – 365 | Sulfate/thiosulfate import ATP-binding protein CysAAdd BLAST | 365 |
Proteomic databases
jPOSTi | P16676 |
PaxDbi | P16676 |
PRIDEi | P16676 |
Interactioni
Subunit structurei
The complex is composed of two ATP-binding proteins (CysA), two transmembrane proteins (CysT and CysW) and a solute-binding protein (CysP).
UniRule annotationBinary interactionsi
P16676
With | #Exp. | IntAct |
---|---|---|
proA [P07004] | 3 | EBI-556404,EBI-548584 |
Protein-protein interaction databases
BioGRIDi | 4260755, 35 interactors 851229, 1 interactor |
ComplexPortali | CPX-4385, Sulfate/thiosulfate ABC transporter complex, cypP variant CPX-4386, Sulfate/thiosulfate ABC transporter complex, sbp variant |
DIPi | DIP-9373N |
IntActi | P16676, 4 interactors |
STRINGi | 511145.b2422 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 3 – 237 | ABC transporterUniRule annotationAdd BLAST | 235 |
Sequence similaritiesi
Belongs to the ABC transporter superfamily. Sulfate/tungstate importer (TC 3.A.1.6) family. [View classification]UniRule annotation
Phylogenomic databases
eggNOGi | COG1118, Bacteria |
HOGENOMi | CLU_000604_1_1_6 |
InParanoidi | P16676 |
PhylomeDBi | P16676 |
Family and domain databases
CDDi | cd03296, ABC_CysA_sulfate_importer, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR014769, ABC_CysA_ATP-bd_C IPR003439, ABC_transporter-like IPR017871, ABC_transporter_CS IPR008995, Mo/tungstate-bd_C_term_dom IPR027417, P-loop_NTPase IPR005666, Sulph_transpt1 |
Pfami | View protein in Pfam PF00005, ABC_tran, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF50331, SSF50331, 1 hit SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00968, 3a0106s01, 1 hit |
PROSITEi | View protein in PROSITE PS00211, ABC_TRANSPORTER_1, 1 hit PS50893, ABC_TRANSPORTER_2, 1 hit PS51237, CYSA, 1 hit |
i Sequence
Sequence statusi: Complete.
P16676-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSIEIANIKK SFGRTQVLND ISLDIPSGQM VALLGPSGSG KTTLLRIIAG
60 70 80 90 100
LEHQTSGHIR FHGTDVSRLH ARDRKVGFVF QHYALFRHMT VFDNIAFGLT
110 120 130 140 150
VLPRRERPNA AAIKAKVTKL LEMVQLAHLA DRYPAQLSGG QKQRVALARA
160 170 180 190 200
LAVEPQILLL DEPFGALDAQ VRKELRRWLR QLHEELKFTS VFVTHDQEEA
210 220 230 240 250
TEVADRVVVM SQGNIEQADA PDQVWREPAT RFVLEFMGEV NRLQGTIRGG
260 270 280 290 300
QFHVGAHRWP LGYTPAYQGP VDLFLRPWEV DISRRTSLDS PLPVQVLEAS
310 320 330 340 350
PKGHYTQLVV QPLGWYNEPL TVVMHGDDAP QRGERLFVGL QHARLYNGDE
360
RIETRDEELA LAQSA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 136 – 137 | QL → HV in AAA23639 (PubMed:2188958).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M32101 Genomic DNA Translation: AAA23639.1 U00096 Genomic DNA Translation: AAC75475.1 AP009048 Genomic DNA Translation: BAA16296.1 |
PIRi | E65016, QRECSA |
RefSeqi | NP_416917.1, NC_000913.3 WP_000021036.1, NZ_SSZK01000005.1 |
Genome annotation databases
EnsemblBacteriai | AAC75475; AAC75475; b2422 BAA16296; BAA16296; BAA16296 |
GeneIDi | 946889 |
KEGGi | ecj:JW2415 eco:b2422 |
PATRICi | fig|1411691.4.peg.4309 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M32101 Genomic DNA Translation: AAA23639.1 U00096 Genomic DNA Translation: AAC75475.1 AP009048 Genomic DNA Translation: BAA16296.1 |
PIRi | E65016, QRECSA |
RefSeqi | NP_416917.1, NC_000913.3 WP_000021036.1, NZ_SSZK01000005.1 |
3D structure databases
SMRi | P16676 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4260755, 35 interactors 851229, 1 interactor |
ComplexPortali | CPX-4385, Sulfate/thiosulfate ABC transporter complex, cypP variant CPX-4386, Sulfate/thiosulfate ABC transporter complex, sbp variant |
DIPi | DIP-9373N |
IntActi | P16676, 4 interactors |
STRINGi | 511145.b2422 |
Protein family/group databases
TCDBi | 3.A.1.6.1, the atp-binding cassette (abc) superfamily |
Proteomic databases
jPOSTi | P16676 |
PaxDbi | P16676 |
PRIDEi | P16676 |
Genome annotation databases
EnsemblBacteriai | AAC75475; AAC75475; b2422 BAA16296; BAA16296; BAA16296 |
GeneIDi | 946889 |
KEGGi | ecj:JW2415 eco:b2422 |
PATRICi | fig|1411691.4.peg.4309 |
Organism-specific databases
EchoBASEi | EB0180 |
Phylogenomic databases
eggNOGi | COG1118, Bacteria |
HOGENOMi | CLU_000604_1_1_6 |
InParanoidi | P16676 |
PhylomeDBi | P16676 |
Enzyme and pathway databases
BioCyci | EcoCyc:CYSA-MONOMER MetaCyc:CYSA-MONOMER |
Miscellaneous databases
PROi | PR:P16676 |
Family and domain databases
CDDi | cd03296, ABC_CysA_sulfate_importer, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR014769, ABC_CysA_ATP-bd_C IPR003439, ABC_transporter-like IPR017871, ABC_transporter_CS IPR008995, Mo/tungstate-bd_C_term_dom IPR027417, P-loop_NTPase IPR005666, Sulph_transpt1 |
Pfami | View protein in Pfam PF00005, ABC_tran, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF50331, SSF50331, 1 hit SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00968, 3a0106s01, 1 hit |
PROSITEi | View protein in PROSITE PS00211, ABC_TRANSPORTER_1, 1 hit PS50893, ABC_TRANSPORTER_2, 1 hit PS51237, CYSA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CYSA_ECOLI | |
Accessioni | P16676Primary (citable) accession number: P16676 Secondary accession number(s): P77693 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1990 |
Last sequence update: | November 1, 1997 | |
Last modified: | April 7, 2021 | |
This is version 190 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families