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Protein

Lysosomal protective protein

Gene

Ctsa

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Protective protein appears to be essential for both the activity of beta-galactosidase and neuraminidase, it associates with these enzymes and exerts a protective function necessary for their stability and activity. This protein is also a carboxypeptidase and can deamidate tachykinins.

Catalytic activityi

Release of a C-terminal amino acid with broad specificity.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei173By similarity1
Active sitei394By similarity1
Active sitei451By similarity1

GO - Molecular functioni

GO - Biological processi

  • negative regulation of chaperone-mediated autophagy Source: ParkinsonsUK-UCL
  • proteolysis Source: ParkinsonsUK-UCL
  • proteolysis involved in cellular protein catabolic process Source: GO_Central
  • regulation of protein stability Source: ParkinsonsUK-UCL

Keywordsi

Molecular functionCarboxypeptidase, Hydrolase, Protease

Enzyme and pathway databases

BRENDAi3.4.16.5 3474
ReactomeiR-MMU-1660662 Glycosphingolipid metabolism
R-MMU-2132295 MHC class II antigen presentation
R-MMU-4085001 Sialic acid metabolism
R-MMU-6798695 Neutrophil degranulation

Protein family/group databases

ESTHERimouse-Ppgb Carboxypeptidase_S10
MEROPSiS10.002

Names & Taxonomyi

Protein namesi
Recommended name:
Lysosomal protective protein (EC:3.4.16.5)
Alternative name(s):
Carboxypeptidase C
Carboxypeptidase L
Cathepsin A
Protective protein cathepsin A
Short name:
PPCA
Protective protein for beta-galactosidase
Cleaved into the following 2 chains:
Gene namesi
Name:Ctsa
Synonyms:Ppgb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:97748 Ctsa

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Lysosome

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3259490

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Add BLAST23
ChainiPRO_000000427724 – 474Lysosomal protective proteinAdd BLAST451
ChainiPRO_000000427824 – 320Lysosomal protective protein 32 kDa chainAdd BLAST297
ChainiPRO_0000004279321 – 474Lysosomal protective protein 20 kDa chainAdd BLAST154

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi83 ↔ 356By similarity
Glycosylationi140N-linked (GlcNAc...) (high mannose) asparagine1 Publication1
Disulfide bondi235 ↔ 251By similarity
Disulfide bondi236 ↔ 241By similarity
Disulfide bondi276 ↔ 325By similarity
Glycosylationi327N-linked (GlcNAc...) (high mannose) asparagine1 Publication1

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

EPDiP16675
PaxDbiP16675
PeptideAtlasiP16675
PRIDEiP16675

PTM databases

iPTMnetiP16675
PhosphoSitePlusiP16675
SwissPalmiP16675
UniCarbKBiP16675

Expressioni

Gene expression databases

BgeeiENSMUSG00000017760
CleanExiMM_CTSA
ExpressionAtlasiP16675 baseline and differential
GenevisibleiP16675 MM

Interactioni

Subunit structurei

Heterodimer of a 32 kDa chain and a 20 kDa chain; disulfide-linked.By similarity

Protein-protein interaction databases

BioGridi202325, 1 interactor
IntActiP16675, 2 interactors
MINTiP16675
STRINGi10090.ENSMUSP00000017904

Structurei

3D structure databases

ProteinModelPortaliP16675
SMRiP16675
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S10 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1282 Eukaryota
COG2939 LUCA
GeneTreeiENSGT00880000138014
HOGENOMiHOG000198295
HOVERGENiHBG053652
InParanoidiP16675
KOiK13289
PhylomeDBiP16675
TreeFamiTF323769

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR001563 Peptidase_S10
IPR033124 Ser_caboxypep_his_AS
IPR018202 Ser_caboxypep_ser_AS
PANTHERiPTHR11802 PTHR11802, 1 hit
PfamiView protein in Pfam
PF00450 Peptidase_S10, 1 hit
PRINTSiPR00724 CRBOXYPTASEC
SUPFAMiSSF53474 SSF53474, 1 hit
PROSITEiView protein in PROSITE
PS00560 CARBOXYPEPT_SER_HIS, 1 hit
PS00131 CARBOXYPEPT_SER_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P16675-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGTALSPLL LLLLLSWASR NEAAPDQDEI DCLPGLAKQP SFRQYSGYLR
60 70 80 90 100
ASDSKHFHYW FVESQNDPKN SPVVLWLNGG PGCSSLDGLL TEHGPFLIQP
110 120 130 140 150
DGVTLEYNPY AWNLIANVLY IESPAGVGFS YSDDKMYVTN DTEVAENNYE
160 170 180 190 200
ALKDFFRLFP EYKDNKLFLT GESYAGIYIP TLAVLVMQDP SMNLQGLAVG
210 220 230 240 250
NGLASYEQND NSLVYFAYYH GLLGNRLWTS LQTHCCAQNK CNFYDNKDPE
260 270 280 290 300
CVNNLLEVSR IVGKSGLNIY NLYAPCAGGV PGRHRYEDTL VVQDFGNIFT
310 320 330 340 350
RLPLKRRFPE ALMRSGDKVR LDPPCTNTTA PSNYLNNPYV RKALHIPESL
360 370 380 390 400
PRWDMCNFLV NLQYRRLYQS MNSQYLKLLS SQKYQILLYN GDVDMACNFM
410 420 430 440 450
GDEWFVDSLN QKMEVQRRPW LVDYGESGEQ VAGFVKECSH ITFLTIKGAG
460 470
HMVPTDKPRA AFTMFSRFLN KEPY
Length:474
Mass (Da):53,844
Last modified:August 1, 1990 - v1
Checksum:i8F4D5A7F4FEBC6CE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti425G → W in AAH18534 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05261 mRNA Translation: AAA39982.1
BC018534 mRNA Translation: AAH18534.1
CCDSiCCDS17062.1
PIRiA35732
RefSeqiNP_001033581.1, NM_001038492.2
UniGeneiMm.359633
Mm.474586

Genome annotation databases

EnsembliENSMUST00000103092; ENSMUSP00000099381; ENSMUSG00000017760
ENSMUST00000103093; ENSMUSP00000099382; ENSMUSG00000017760
GeneIDi19025
KEGGimmu:19025
UCSCiuc008nwm.2 mouse

Similar proteinsi

Entry informationi

Entry nameiPPGB_MOUSE
AccessioniPrimary (citable) accession number: P16675
Secondary accession number(s): Q8VEF6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: May 23, 2018
This is version 165 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

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