UniProtKB - P16659 (SYP_ECOLI)
Proline--tRNA ligase
proS
Functioni
Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). Misacylated Cys-tRNA(Pro) is not edited by ProRS, but instead may be edited in trans by YbaK.
Catalytic activityi
- EC:6.1.1.15
Kineticsi
- KM=0.25 mM for proline3 Publications
- KM=140 mM for alanine3 Publications
- KM=0.17 mM for cysteine3 Publications
GO - Molecular functioni
- Ala-tRNA(Pro) hydrolase activity Source: UniProtKB
- ATP binding Source: UniProtKB-UniRule
- proline-tRNA ligase activity Source: EcoCyc
GO - Biological processi
- prolyl-tRNA aminoacylation Source: EcoCyc
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:PROS-MONOMER |
BRENDAi | 6.1.1.15, 2026 |
SABIO-RKi | P16659 |
Names & Taxonomyi
Protein namesi | Recommended name: Proline--tRNA ligase (EC:6.1.1.15)Alternative name(s): Global RNA synthesis factor Prolyl-tRNA synthetase Short name: ProRS |
Gene namesi | Name:proS Synonyms:drpA Ordered Locus Names:b0194, JW0190 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
Cytosol
- cytosol Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 257 | T → A: Reduces the posttransfer editing activity 5-fold, with little change in both the aminoacylation and the pretransfer editing activities. 1 Publication | 1 | |
Mutagenesisi | 279 | K → A: Severely affects the posttransfer editing activity, with little change in both the aminoacylation and the pretransfer editing activities. 1 Publication | 1 | |
Mutagenesisi | 350 | D → A: Abolishes the pretransfer editing activity and reduces the aminoacylation activity 20-fold and the posttransfer editing activity 5-fold. 1 Publication | 1 | |
Mutagenesisi | 369 | H → A: Reduces both the aminoacylation and the pretransfer editing activities 2.5-fold, and the posttransfer editing activity 5-fold. Loss of specificity in deacylation. 1 Publication | 1 | |
Mutagenesisi | 378 | D → A: Little change in both the aminoacylation and the editing activities. 1 Publication | 1 | |
Mutagenesisi | 386 | D → A: Reduces the posttransfer editing activity approximately 2-fold, with little change in both the aminoacylation and the pretransfer editing activities. 1 Publication | 1 | |
Mutagenesisi | 394 | D → A: Reduces the posttransfer editing activity approximately 2-fold, with little change in both the aminoacylation and the pretransfer editing activities. 1 Publication | 1 | |
Mutagenesisi | 443 | C → G: 150-fold decrease in posttransfer editing activity against alanine, without change neither in pretransfer editing nor in aminoacylation. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000139329 | 1 – 572 | Proline--tRNA ligaseAdd BLAST | 572 |
Proteomic databases
jPOSTi | P16659 |
PaxDbi | P16659 |
PRIDEi | P16659 |
2D gel databases
SWISS-2DPAGEi | P16659 |
PTM databases
CarbonylDBi | P16659 |
Interactioni
Subunit structurei
Homodimer.
Protein-protein interaction databases
BioGRIDi | 4261629, 32 interactors |
DIPi | DIP-10573N |
IntActi | P16659, 20 interactors |
STRINGi | 511145.b0194 |
Family & Domainsi
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0442, Bacteria |
HOGENOMi | CLU_016739_0_0_6 |
InParanoidi | P16659 |
PhylomeDBi | P16659 |
Family and domain databases
CDDi | cd00861, ProRS_anticodon_short, 1 hit cd00779, ProRS_core_prok, 1 hit |
Gene3Di | 3.30.930.10, 2 hits 3.40.50.800, 1 hit 3.90.960.10, 1 hit |
HAMAPi | MF_01569, Pro_tRNA_synth_type1, 1 hit |
InterProi | View protein in InterPro IPR002314, aa-tRNA-synt_IIb IPR006195, aa-tRNA-synth_II IPR045864, aa-tRNA-synth_II/BPL/LPL IPR004154, Anticodon-bd IPR036621, Anticodon-bd_dom_sf IPR002316, Pro-tRNA-ligase_IIa IPR004500, Pro-tRNA-synth_IIa_bac-type IPR023717, Pro-tRNA-Synthase_IIa_type1 IPR044140, ProRS_anticodon_short IPR033730, ProRS_core_prok IPR036754, YbaK/aa-tRNA-synt-asso_dom_sf IPR007214, YbaK/aa-tRNA-synth-assoc-dom |
Pfami | View protein in Pfam PF03129, HGTP_anticodon, 1 hit PF00587, tRNA-synt_2b, 1 hit PF04073, tRNA_edit, 1 hit |
PIRSFi | PIRSF001535, ProRS_1, 1 hit |
PRINTSi | PR01046, TRNASYNTHPRO |
SUPFAMi | SSF55681, SSF55681, 1 hit SSF55826, SSF55826, 1 hit |
TIGRFAMsi | TIGR00409, proS_fam_II, 1 hit |
PROSITEi | View protein in PROSITE PS50862, AA_TRNA_LIGASE_II, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MRTSQYLLST LKETPADAEV ISHQLMLRAG MIRKLASGLY TWLPTGVRVL
60 70 80 90 100
KKVENIVREE MNNAGAIEVS MPVVQPADLW QESGRWEQYG PELLRFVDRG
110 120 130 140 150
ERPFVLGPTH EEVITDLIRN ELSSYKQLPL NFYQIQTKFR DEVRPRFGVM
160 170 180 190 200
RSREFLMKDA YSFHTSQESL QETYDAMYAA YSKIFSRMGL DFRAVQADTG
210 220 230 240 250
SIGGSASHEF QVLAQSGEDD VVFSDTSDYA ANIELAEAIA PKEPRAAATQ
260 270 280 290 300
EMTLVDTPNA KTIAELVEQF NLPIEKTVKT LLVKAVEGSS FPQVALLVRG
310 320 330 340 350
DHELNEVKAE KLPQVASPLT FATEEEIRAV VKAGPGSLGP VNMPIPVVID
360 370 380 390 400
RTVAAMSDFA AGANIDGKHY FGINWDRDVA TPEVADIRNV VAGDPSPDGQ
410 420 430 440 450
GRLLIKRGIE VGHIFQLGTK YSEALKASVQ GEDGRNQILT MGCYGIGVTR
460 470 480 490 500
VVAAAIEQNY DERGIVWPDA IAPFQVAILP MNMHKSFRVQ ELAEKLYSEL
510 520 530 540 550
RAQGIEVLLD DRKERPGVMF ADMELIGIPH TIVLGDRNLD NDDIEYKYRR
560 570
NGEKQLIKTG DIVEYLVKQI KG
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 26 – 27 | ML → IV (PubMed:2203971).Curated | 2 | |
Sequence conflicti | 127 – 128 | QL → HV in AAA23710 (PubMed:1688424).Curated | 2 | |
Sequence conflicti | 205 – 216 | SASHE…VLAQS → RPLTNSRCWRR in AAA23710 (PubMed:1688424).CuratedAdd BLAST | 12 | |
Sequence conflicti | 205 | S → Q (PubMed:2203971).Curated | 1 | |
Sequence conflicti | 516 – 517 | PG → RA (PubMed:1688424).Curated | 2 | |
Sequence conflicti | 518 – 572 | Missing (PubMed:1688424).CuratedAdd BLAST | 55 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X55518 Genomic DNA Translation: CAA39134.1 M97858 Genomic DNA Translation: AAA24420.1 M32357 Genomic DNA Translation: AAA23710.1 U70214 Genomic DNA Translation: AAB08622.1 U00096 Genomic DNA Translation: AAC73305.1 AP009048 Genomic DNA Translation: BAA77870.2 D15061 Genomic DNA Translation: BAA03654.1 |
PIRi | B64744, YPEC |
RefSeqi | NP_414736.1, NC_000913.3 WP_001260717.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC73305; AAC73305; b0194 BAA77870; BAA77870; BAA77870 |
GeneIDi | 949116 |
KEGGi | ecj:JW0190 eco:b0194 |
PATRICi | fig|1411691.4.peg.2084 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X55518 Genomic DNA Translation: CAA39134.1 M97858 Genomic DNA Translation: AAA24420.1 M32357 Genomic DNA Translation: AAA23710.1 U70214 Genomic DNA Translation: AAB08622.1 U00096 Genomic DNA Translation: AAC73305.1 AP009048 Genomic DNA Translation: BAA77870.2 D15061 Genomic DNA Translation: BAA03654.1 |
PIRi | B64744, YPEC |
RefSeqi | NP_414736.1, NC_000913.3 WP_001260717.1, NZ_LN832404.1 |
3D structure databases
SMRi | P16659 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4261629, 32 interactors |
DIPi | DIP-10573N |
IntActi | P16659, 20 interactors |
STRINGi | 511145.b0194 |
PTM databases
CarbonylDBi | P16659 |
2D gel databases
SWISS-2DPAGEi | P16659 |
Proteomic databases
jPOSTi | P16659 |
PaxDbi | P16659 |
PRIDEi | P16659 |
Genome annotation databases
EnsemblBacteriai | AAC73305; AAC73305; b0194 BAA77870; BAA77870; BAA77870 |
GeneIDi | 949116 |
KEGGi | ecj:JW0190 eco:b0194 |
PATRICi | fig|1411691.4.peg.2084 |
Organism-specific databases
EchoBASEi | EB0763 |
Phylogenomic databases
eggNOGi | COG0442, Bacteria |
HOGENOMi | CLU_016739_0_0_6 |
InParanoidi | P16659 |
PhylomeDBi | P16659 |
Enzyme and pathway databases
BioCyci | EcoCyc:PROS-MONOMER |
BRENDAi | 6.1.1.15, 2026 |
SABIO-RKi | P16659 |
Miscellaneous databases
PROi | PR:P16659 |
Family and domain databases
CDDi | cd00861, ProRS_anticodon_short, 1 hit cd00779, ProRS_core_prok, 1 hit |
Gene3Di | 3.30.930.10, 2 hits 3.40.50.800, 1 hit 3.90.960.10, 1 hit |
HAMAPi | MF_01569, Pro_tRNA_synth_type1, 1 hit |
InterProi | View protein in InterPro IPR002314, aa-tRNA-synt_IIb IPR006195, aa-tRNA-synth_II IPR045864, aa-tRNA-synth_II/BPL/LPL IPR004154, Anticodon-bd IPR036621, Anticodon-bd_dom_sf IPR002316, Pro-tRNA-ligase_IIa IPR004500, Pro-tRNA-synth_IIa_bac-type IPR023717, Pro-tRNA-Synthase_IIa_type1 IPR044140, ProRS_anticodon_short IPR033730, ProRS_core_prok IPR036754, YbaK/aa-tRNA-synt-asso_dom_sf IPR007214, YbaK/aa-tRNA-synth-assoc-dom |
Pfami | View protein in Pfam PF03129, HGTP_anticodon, 1 hit PF00587, tRNA-synt_2b, 1 hit PF04073, tRNA_edit, 1 hit |
PIRSFi | PIRSF001535, ProRS_1, 1 hit |
PRINTSi | PR01046, TRNASYNTHPRO |
SUPFAMi | SSF55681, SSF55681, 1 hit SSF55826, SSF55826, 1 hit |
TIGRFAMsi | TIGR00409, proS_fam_II, 1 hit |
PROSITEi | View protein in PROSITE PS50862, AA_TRNA_LIGASE_II, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SYP_ECOLI | |
Accessioni | P16659Primary (citable) accession number: P16659 Secondary accession number(s): P78272, Q59430 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1990 |
Last sequence update: | August 29, 2003 | |
Last modified: | February 23, 2022 | |
This is version 189 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Aminoacyl-tRNA synthetases
List of aminoacyl-tRNA synthetase entries - SIMILARITY comments
Index of protein domains and families