UniProtKB - P16649 (TUP1_YEAST)
Protein
General transcriptional corepressor TUP1
Gene
TUP1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Acts as component of the CYC8-TUP1 corepressor complex which is involved in the repression of many genes in a wide variety of physiological processes including heme-regulated and catabolite repressed genes. May also be involved in the derepression of at least some target genes. The complex is recruited to target genes by interaction with DNA-bound transcriptional repressors, like MATALPHA2, MIG1, RFX1 and SKO1. The complex recruits histone deacetylases to produce a repressive chromatin structure, interacts with hypoacetylated N-terminal tails of histones H3 and H4 that have been programmed for repression by the action of histone deacetylases and interferes directly with the transcriptional machinery by associating with the RNA polymerase II mediator complex.7 Publications
Miscellaneous
Present with 5840 molecules/cell in log phase SD medium.1 Publication
GO - Molecular functioni
- histone binding Source: SGD
- histone deacetylase binding Source: SGD
- mediator complex binding Source: SGD
- phosphatidylinositol-3,5-bisphosphate binding Source: SGD
- transcription corepressor activity Source: SGD
GO - Biological processi
- carbon catabolite repression of transcription from RNA polymerase II promoter by glucose Source: SGD
- histone exchange Source: SGD
- negative regulation of mating-type specific transcription from RNA polymerase II promoter Source: SGD
- negative regulation of transcription by RNA polymerase II Source: SGD
- nucleosome positioning Source: SGD
- positive regulation of single-species biofilm formation Source: SGD
- positive regulation of transcription by RNA polymerase II Source: SGD
- regulation of flocculation Source: SGD
- regulation of invasive growth in response to glucose limitation Source: SGD
- regulation of response to DNA damage stimulus Source: SGD
- regulation of transcription by RNA polymerase II Source: SGD
Keywordsi
Molecular function | Repressor |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: General transcriptional corepressor TUP1Alternative name(s): Flocculation suppressor protein Glucose repression regulatory protein TUP1 Repressor AER2 |
Gene namesi | Name:TUP1 Synonyms:AAR1, AER2, AMM1, CYC9, FLK1, SFL2, UMR7 Ordered Locus Names:YCR084C ORF Names:YCR84C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YCR084C |
SGDi | S000000680, TUP1 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Nucleus
- nucleus Source: SGD
Other locations
- transcription repressor complex Source: SGD
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000051312 | 1 – 713 | General transcriptional corepressor TUP1Add BLAST | 713 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 439 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P16649 |
PaxDbi | P16649 |
PRIDEi | P16649 |
PTM databases
iPTMneti | P16649 |
Interactioni
Subunit structurei
Associates with CYC8/SSN6 to form the CYC8-TUP1 (or TUP1-SSN6) corepressor complex that is composed of 4 copies of TUP1 and one copy of CYC8.
Interacts with histone H3, histone H4, MATALPHA2, RFX1, SKO1, PGD1, HDA1, HDA2, HOS1, HOS2 AND RPD3.
9 PublicationsBinary interactionsi
Hide detailsP16649
With | #Exp. | IntAct |
---|---|---|
CYC8 [P14922] | 6 | EBI-19654,EBI-18215 |
PGD1 [P40356] | 3 | EBI-19654,EBI-13268 |
RFX1 [P48743] | 2 | EBI-19654,EBI-15036 |
RPD3 [P32561] | 2 | EBI-19654,EBI-15864 |
GO - Molecular functioni
- histone binding Source: SGD
- histone deacetylase binding Source: SGD
Protein-protein interaction databases
BioGRIDi | 31057, 110 interactors |
ComplexPortali | CPX-1663, CYP8-TUP1 corepressor complex |
DIPi | DIP-512N |
IntActi | P16649, 27 interactors |
MINTi | P16649 |
STRINGi | 4932.YCR084C |
Miscellaneous databases
RNActi | P16649, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P16649 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P16649 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 342 – 371 | WD 1Add BLAST | 30 | |
Repeati | 441 – 471 | WD 2Add BLAST | 31 | |
Repeati | 483 – 513 | WD 3Add BLAST | 31 | |
Repeati | 524 – 555 | WD 4Add BLAST | 32 | |
Repeati | 574 – 604 | WD 5Add BLAST | 31 | |
Repeati | 628 – 658 | WD 6Add BLAST | 31 | |
Repeati | 670 – 706 | WD 7Add BLAST | 37 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 97 – 118 | Gln-richAdd BLAST | 22 | |
Compositional biasi | 181 – 198 | Poly-GlnAdd BLAST | 18 | |
Compositional biasi | 399 – 409 | Thr-richAdd BLAST | 11 |
Sequence similaritiesi
Belongs to the WD repeat TUP1 family.Curated
Keywords - Domaini
Repeat, WD repeatPhylogenomic databases
eggNOGi | KOG0266, Eukaryota |
GeneTreei | ENSGT00960000189238 |
HOGENOMi | CLU_000288_57_23_1 |
InParanoidi | P16649 |
OMAi | MEQTHMT |
Family and domain databases
Gene3Di | 2.130.10.10, 2 hits |
InterProi | View protein in InterPro IPR020472, G-protein_beta_WD-40_rep IPR013890, Tscrpt_rep_Tup1_N IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR019775, WD40_repeat_CS IPR017986, WD40_repeat_dom IPR036322, WD40_repeat_dom_sf |
Pfami | View protein in Pfam PF08581, Tup_N, 1 hit PF00400, WD40, 7 hits |
PRINTSi | PR00320, GPROTEINBRPT |
SMARTi | View protein in SMART SM00320, WD40, 7 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS00678, WD_REPEATS_1, 4 hits PS50082, WD_REPEATS_2, 5 hits PS50294, WD_REPEATS_REGION, 1 hit |
i Sequence
Sequence statusi: Complete.
P16649-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTASVSNTQN KLNELLDAIR QEFLQVSQEA NTYRLQNQKD YDFKMNQQLA
60 70 80 90 100
EMQQIRNTVY ELELTHRKMK DAYEEEIKHL KLGLEQRDHQ IASLTVQQQR
110 120 130 140 150
QQQQQQQVQQ HLQQQQQQLA AASASVPVAQ QPPATTSATA TPAANTTTGS
160 170 180 190 200
PSAFPVQASR PNLVGSQLPT TTLPVVSSNA QQQLPQQQLQ QQQLQQQQPP
210 220 230 240 250
PQVSVAPLSN TAINGSPTSK ETTTLPSVKA PESTLKETEP ENNNTSKIND
260 270 280 290 300
TGSATTATTT TATETEIKPK EEDATPASLH QDHYLVPYNQ RANHSKPIPP
310 320 330 340 350
FLLDLDSQSV PDALKKQTND YYILYNPALP REIDVELHKS LDHTSVVCCV
360 370 380 390 400
KFSNDGEYLA TGCNKTTQVY RVSDGSLVAR LSDDSAANNH RNSITENNTT
410 420 430 440 450
TSTDNNTMTT TTTTTITTTA MTSAAELAKD VENLNTSSSP SSDLYIRSVC
460 470 480 490 500
FSPDGKFLAT GAEDRLIRIW DIENRKIVMI LQGHEQDIYS LDYFPSGDKL
510 520 530 540 550
VSGSGDRTVR IWDLRTGQCS LTLSIEDGVT TVAVSPGDGK YIAAGSLDRA
560 570 580 590 600
VRVWDSETGF LVERLDSENE SGTGHKDSVY SVVFTRDGQS VVSGSLDRSV
610 620 630 640 650
KLWNLQNANN KSDSKTPNSG TCEVTYIGHK DFVLSVATTQ NDEYILSGSK
660 670 680 690 700
DRGVLFWDKK SGNPLLMLQG HRNSVISVAV ANGSPLGPEY NVFATGSGDC
710
KARIWKYKKI APN
Sequence cautioni
The sequence CAA34411 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 75 | E → A in AAA35182 (PubMed:2247069).Curated | 1 | |
Sequence conflicti | 100 | R → Q in AAA35182 (PubMed:2247069).Curated | 1 | |
Sequence conflicti | 685 | P → S in AAA35182 (PubMed:2247069).Curated | 1 | |
Sequence conflicti | 685 | P → S in AAA34413 (PubMed:1900249).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M31733 Genomic DNA Translation: AAA35182.1 M35861 Genomic DNA Translation: AAA34413.1 X16365 Genomic DNA Translation: CAA34411.1 Different initiation. X59720 Genomic DNA Translation: CAA42259.1 BK006937 Genomic DNA Translation: DAA07554.1 |
PIRi | JN0133 |
RefSeqi | NP_010007.1, NM_001178790.1 |
Genome annotation databases
EnsemblFungii | YCR084C_mRNA; YCR084C; YCR084C |
GeneIDi | 850445 |
KEGGi | sce:YCR084C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M31733 Genomic DNA Translation: AAA35182.1 M35861 Genomic DNA Translation: AAA34413.1 X16365 Genomic DNA Translation: CAA34411.1 Different initiation. X59720 Genomic DNA Translation: CAA42259.1 BK006937 Genomic DNA Translation: DAA07554.1 |
PIRi | JN0133 |
RefSeqi | NP_010007.1, NM_001178790.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1ERJ | X-ray | 2.30 | A/B/C | 282-388 | [»] | |
A/B/C | 432-713 | [»] | ||||
3VP8 | X-ray | 1.91 | A/B/C/D | 1-92 | [»] | |
3VP9 | X-ray | 1.80 | A/B | 1-92 | [»] | |
SMRi | P16649 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 31057, 110 interactors |
ComplexPortali | CPX-1663, CYP8-TUP1 corepressor complex |
DIPi | DIP-512N |
IntActi | P16649, 27 interactors |
MINTi | P16649 |
STRINGi | 4932.YCR084C |
PTM databases
iPTMneti | P16649 |
Proteomic databases
MaxQBi | P16649 |
PaxDbi | P16649 |
PRIDEi | P16649 |
Genome annotation databases
EnsemblFungii | YCR084C_mRNA; YCR084C; YCR084C |
GeneIDi | 850445 |
KEGGi | sce:YCR084C |
Organism-specific databases
EuPathDBi | FungiDB:YCR084C |
SGDi | S000000680, TUP1 |
Phylogenomic databases
eggNOGi | KOG0266, Eukaryota |
GeneTreei | ENSGT00960000189238 |
HOGENOMi | CLU_000288_57_23_1 |
InParanoidi | P16649 |
OMAi | MEQTHMT |
Miscellaneous databases
EvolutionaryTracei | P16649 |
PROi | PR:P16649 |
RNActi | P16649, protein |
Family and domain databases
Gene3Di | 2.130.10.10, 2 hits |
InterProi | View protein in InterPro IPR020472, G-protein_beta_WD-40_rep IPR013890, Tscrpt_rep_Tup1_N IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR019775, WD40_repeat_CS IPR017986, WD40_repeat_dom IPR036322, WD40_repeat_dom_sf |
Pfami | View protein in Pfam PF08581, Tup_N, 1 hit PF00400, WD40, 7 hits |
PRINTSi | PR00320, GPROTEINBRPT |
SMARTi | View protein in SMART SM00320, WD40, 7 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS00678, WD_REPEATS_1, 4 hits PS50082, WD_REPEATS_2, 5 hits PS50294, WD_REPEATS_REGION, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TUP1_YEAST | |
Accessioni | P16649Primary (citable) accession number: P16649 Secondary accession number(s): D6VR85, P17995, P18323 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1990 |
Last sequence update: | May 1, 1991 | |
Last modified: | December 2, 2020 | |
This is version 218 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome III
Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names