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Protein

Transcriptional repressor IclR

Gene

iclR

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulation of the glyoxylate bypass operon (aceBAK), which encodes isocitrate lyase, malate synthase as well as isocitrate dehydrogenase kinase/phosphorylase. Glyoxylate disrupts the interaction with the promoter by favoring the inactive dimeric form. Pyruvate enhances promoter binding by stabilizing the tetrameric form.

Miscellaneous

Glyoxylate and pyruvate occupy the same site.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei212Glyoxylate1
Binding sitei222Glyoxylate1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi46 – 65H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

  • DNA binding Source: GO_Central
  • DNA binding transcription factor activity Source: GO_Central
  • identical protein binding Source: IntAct

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processGlyoxylate bypass, Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciEcoCyc:PD04099

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional repressor IclRCurated
Short name:
Acetate operon repressor
Gene namesi
Name:iclR
Ordered Locus Names:b4018, JW3978
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10491 iclR

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi143L → D: Strongly reduced promoter binding and tetramerization. 1 Publication1
Mutagenesisi146M → D: Strongly reduced promoter binding and tetramerization. 1 Publication1
Mutagenesisi154L → D: Strongly reduced promoter binding and tetramerization. 1 Publication1
Mutagenesisi220L → D: Loss of promoter binding and tetramerization. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002017581 – 274Transcriptional repressor IclRAdd BLAST274

Proteomic databases

EPDiP16528
PaxDbiP16528
PRIDEiP16528

Interactioni

Subunit structurei

Homotetramer and homodimer. Homotetramer in its active, DNA-bound form. Homodimer in its inactive form.1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi4263460, 10 interactors
DIPiDIP-10008N
IntActiP16528, 17 interactors
MINTiP16528
STRINGi316385.ECDH10B_4207

Structurei

Secondary structure

1274
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi99 – 116Combined sources18
Beta strandi118 – 126Combined sources9
Turni127 – 130Combined sources4
Beta strandi131 – 138Combined sources8
Beta strandi143 – 146Combined sources4
Beta strandi156 – 159Combined sources4
Helixi160 – 166Combined sources7
Helixi171 – 175Combined sources5
Helixi177 – 180Combined sources4
Helixi194 – 207Combined sources14
Beta strandi209 – 217Combined sources9
Beta strandi220 – 228Combined sources9
Beta strandi230 – 232Combined sources3
Beta strandi234 – 243Combined sources10
Turni244 – 246Combined sources3
Helixi249 – 251Combined sources3
Helixi252 – 273Combined sources22

3D structure databases

ProteinModelPortaliP16528
SMRiP16528
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP16528

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 86HTH iclR-typePROSITE-ProRule annotationAdd BLAST63
Domaini101 – 272IclR-EDPROSITE-ProRule annotationAdd BLAST172

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni160 – 161Glyoxylate binding2
Regioni239 – 241Glyoxylate binding3

Phylogenomic databases

eggNOGiENOG41069M3 Bacteria
COG1414 LUCA
HOGENOMiHOG000107041
InParanoidiP16528
KOiK13641
OMAiEPDRLHR
PhylomeDBiP16528

Family and domain databases

Gene3Di1.10.10.10, 1 hit
3.30.450.40, 1 hit
InterProiView protein in InterPro
IPR029016 GAF-like_dom_sf
IPR014757 Tscrpt_reg_IclR_C
IPR005471 Tscrpt_reg_IclR_N
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF09339 HTH_IclR, 1 hit
PF01614 IclR, 1 hit
SMARTiView protein in SMART
SM00346 HTH_ICLR, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
PROSITEiView protein in PROSITE
PS51077 HTH_ICLR, 1 hit
PS51078 ICLR_ED, 1 hit

Sequencei

Sequence statusi: Complete.

P16528-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVAPIPAKRG RKPAVATAPA TGQVQSLTRG LKLLEWIAES NGSVALTELA
60 70 80 90 100
QQAGLPNSTT HRLLTTMQQQ GFVRQVGELG HWAIGAHAFM VGSSFLQSRN
110 120 130 140 150
LLAIVHPILR NLMEESGETV NMAVLDQSDH EAIIIDQVQC THLMRMSAPI
160 170 180 190 200
GGKLPMHASG AGKAFLAQLS EEQVTKLLHR KGLHAYTHAT LVSPVHLKED
210 220 230 240 250
LAQTRKRGYS FDDEEHALGL RCLAACIFDE HREPFAAISI SGPISRITDD
260 270
RVTEFGAMVI KAAKEVTLAY GGMR
Length:274
Mass (Da):29,739
Last modified:August 1, 1990 - v1
Checksum:i7C8A7E9FD2841D0C
GO

Sequence cautioni

The sequence AAA50561 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAC43112 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31761 Genomic DNA Translation: AAA24008.1
M63914 Genomic DNA Translation: AAA50561.1 Different initiation.
U00006 Genomic DNA Translation: AAC43112.1 Different initiation.
U00096 Genomic DNA Translation: AAC76988.2
AP009048 Genomic DNA Translation: BAE78020.1
M63497 Genomic DNA Translation: AAA73003.1
RefSeqiNP_418442.2, NC_000913.3
WP_000226403.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76988; AAC76988; b4018
BAE78020; BAE78020; BAE78020
GeneIDi948524
KEGGiecj:JW3978
eco:b4018
PATRICifig|1411691.4.peg.2695

Similar proteinsi

Entry informationi

Entry nameiICLR_ECOLI
AccessioniPrimary (citable) accession number: P16528
Secondary accession number(s): P76782, Q2M6T6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: July 18, 2018
This is version 164 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

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