UniProtKB - P16525 (TUS_ECOLI)
Protein
DNA replication terminus site-binding protein
Gene
tus
Organism
Escherichia coli (strain K12)
Status
Functioni
Trans-acting protein required for termination of DNA replication. Binds to DNA replication terminator sequences (terA to terF) to prevent the passage of replication forks. The termination efficiency will be affected by the affinity of this protein for the terminator sequence.
GO - Molecular functioni
- sequence-specific DNA binding Source: EcoCyc
GO - Biological processi
- DNA replication termination Source: EcoCyc
- replication fork arrest involved in DNA replication termination Source: EcoCyc
Keywordsi
Molecular function | DNA-binding |
Biological process | DNA replication |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11038-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: DNA replication terminus site-binding proteinUniRule annotationShort name: Ter-binding proteinUniRule annotation |
Gene namesi | Name:tusUniRule annotation Synonyms:tau Ordered Locus Names:b1610, JW1602 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000049413 | 1 – 309 | DNA replication terminus site-binding proteinAdd BLAST | 309 |
Proteomic databases
jPOSTi | P16525 |
PaxDbi | P16525 |
PRIDEi | P16525 |
Interactioni
Subunit structurei
Monomer.
Protein-protein interaction databases
BioGRIDi | 4260254, 85 interactors 849524, 1 interactor |
DIPi | DIP-11056N |
IntActi | P16525, 2 interactors |
MINTi | P16525 |
STRINGi | 511145.b1610 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P16525 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P16525 |
Family & Domainsi
Sequence similaritiesi
Belongs to the Tus family.UniRule annotation
Phylogenomic databases
eggNOGi | ENOG502Z895, Bacteria |
HOGENOMi | CLU_078181_0_0_6 |
Family and domain databases
Gene3Di | 3.50.14.10, 1 hit |
HAMAPi | MF_00483, Rep_term_Tus, 1 hit |
InterProi | View protein in InterPro IPR008865, DNA_replication_term_site-bd IPR036381, Tus_dom1 IPR036384, Tus_sf |
Pfami | View protein in Pfam PF05472, Ter, 1 hit |
SUPFAMi | SSF56596, SSF56596, 1 hit |
TIGRFAMsi | TIGR02648, rep_term_tus, 1 hit |
i Sequence
Sequence statusi: Complete.
P16525-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MARYDLVDRL NTTFRQMEQE LAIFAAHLEQ HKLLVARVFS LPEVKKEDEH
60 70 80 90 100
NPLNRIEVKQ HLGNDAQSLA LRHFRHLFIQ QQSENRSSKA AVRLPGVLCY
110 120 130 140 150
QVDNLSQAAL VSHIQHINKL KTTFEHIVTV ESELPTAARF EWVHRHLPGL
160 170 180 190 200
ITLNAYRTLT VLHDPATLRF GWANKHIIKN LHRDEVLAQL EKSLKSPRSV
210 220 230 240 250
APWTREEWQR KLEREYQDIA ALPQNAKLKI KRPVKVQPIA RVWYKGDQKQ
260 270 280 290 300
VQHACPTPLI ALINRDNGAG VPDVGELLNY DADNVQHRYK PQAQPLRLII
PRLHLYVAD
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 85 – 102 | NRSSK…LCYQV → SQQQGHCPSAWLLCSGS in CAA27699 (PubMed:3541901).CuratedAdd BLAST | 18 | |
Sequence conflicti | 121 – 122 | KT → EA in CAA27699 (PubMed:3541901).Curated | 2 | |
Sequence conflicti | 134 | L → I in CAA27699 (PubMed:3541901).Curated | 1 | |
Sequence conflicti | 150 | L → V in CAA27699 (PubMed:3541901).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D90037 Genomic DNA Translation: BAA14085.1 U41101 Genomic DNA Translation: AAA82083.1 U00096 Genomic DNA Translation: AAC74682.1 AP009048 Genomic DNA Translation: BAA15348.1 X04065 Genomic DNA Translation: CAA27699.1 |
PIRi | B32161, DNECTS |
RefSeqi | NP_416127.1, NC_000913.3 WP_000135181.1, NZ_SSZK01000001.1 |
Genome annotation databases
EnsemblBacteriai | AAC74682; AAC74682; b1610 BAA15348; BAA15348; BAA15348 |
GeneIDi | 57731004 945135 |
KEGGi | ecj:JW1602 eco:b1610 |
PATRICi | fig|1411691.4.peg.652 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D90037 Genomic DNA Translation: BAA14085.1 U41101 Genomic DNA Translation: AAA82083.1 U00096 Genomic DNA Translation: AAC74682.1 AP009048 Genomic DNA Translation: BAA15348.1 X04065 Genomic DNA Translation: CAA27699.1 |
PIRi | B32161, DNECTS |
RefSeqi | NP_416127.1, NC_000913.3 WP_000135181.1, NZ_SSZK01000001.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1ECR | X-ray | 2.70 | A | 1-309 | [»] | |
1SUT | NMR | - | A | 223-244 | [»] | |
2EWJ | X-ray | 2.70 | A | 1-309 | [»] | |
2I05 | X-ray | 2.60 | A | 1-309 | [»] | |
2I06 | X-ray | 2.20 | A | 1-309 | [»] | |
4XR0 | X-ray | 2.80 | A | 1-309 | [»] | |
4XR1 | X-ray | 2.40 | A | 1-309 | [»] | |
4XR2 | X-ray | 2.35 | A | 1-309 | [»] | |
4XR3 | X-ray | 2.70 | A | 1-309 | [»] | |
SMRi | P16525 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260254, 85 interactors 849524, 1 interactor |
DIPi | DIP-11056N |
IntActi | P16525, 2 interactors |
MINTi | P16525 |
STRINGi | 511145.b1610 |
Proteomic databases
jPOSTi | P16525 |
PaxDbi | P16525 |
PRIDEi | P16525 |
Genome annotation databases
EnsemblBacteriai | AAC74682; AAC74682; b1610 BAA15348; BAA15348; BAA15348 |
GeneIDi | 57731004 945135 |
KEGGi | ecj:JW1602 eco:b1610 |
PATRICi | fig|1411691.4.peg.652 |
Organism-specific databases
EchoBASEi | EB1031 |
Phylogenomic databases
eggNOGi | ENOG502Z895, Bacteria |
HOGENOMi | CLU_078181_0_0_6 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11038-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P16525 |
PROi | PR:P16525 |
Family and domain databases
Gene3Di | 3.50.14.10, 1 hit |
HAMAPi | MF_00483, Rep_term_Tus, 1 hit |
InterProi | View protein in InterPro IPR008865, DNA_replication_term_site-bd IPR036381, Tus_dom1 IPR036384, Tus_sf |
Pfami | View protein in Pfam PF05472, Ter, 1 hit |
SUPFAMi | SSF56596, SSF56596, 1 hit |
TIGRFAMsi | TIGR02648, rep_term_tus, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TUS_ECOLI | |
Accessioni | P16525Primary (citable) accession number: P16525 Secondary accession number(s): Q59400 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1990 |
Last sequence update: | August 1, 1990 | |
Last modified: | February 10, 2021 | |
This is version 168 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families