UniProtKB - P16497 (KINA_BACSU)
Protein
Sporulation kinase A
Gene
kinA
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Phosphorylates the sporulation-regulatory proteins spo0A and spo0F. It also autophosphorylates in the presence of ATP.
Catalytic activityi
- ATP + protein L-histidine = ADP + protein N-phospho-L-histidine. EC:2.7.13.3
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- identical protein binding Source: IntAct
- phosphorelay sensor kinase activity Source: InterPro
GO - Biological processi
- regulation of transcription, DNA-templated Source: InterPro
- sporulation resulting in formation of a cellular spore Source: CACAO
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Sporulation, Two-component regulatory system |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | BSUB:BSU13990-MONOMER |
BRENDAi | 2.7.13.3, 658 |
Names & Taxonomyi
Protein namesi | Recommended name: Sporulation kinase A (EC:2.7.13.3)Alternative name(s): Stage II sporulation protein F Stage II sporulation protein J |
Gene namesi | Name:kinA Synonyms:gsiC, scoD, spoIIF, spoIIJ Ordered Locus Names:BSU13990 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000074776 | 1 – 606 | Sporulation kinase AAdd BLAST | 606 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 405 | Phosphohistidine; by autocatalysisPROSITE-ProRule annotation | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P16497 |
PRIDEi | P16497 |
Interactioni
Binary interactionsi
Hide detailsP16497
With | #Exp. | IntAct |
---|---|---|
itself | 8 | EBI-6405707,EBI-6405707 |
sda [Q7WY62] | 6 | EBI-6405707,EBI-6405714 |
spo0F [P06628] | 3 | EBI-6405707,EBI-6418009 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
DIPi | DIP-58966N |
IntActi | P16497, 2 interactors |
STRINGi | 224308.BSU13990 |
Chemistry databases
BindingDBi | P16497 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SASBDBi | P16497 |
SMRi | P16497 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P16497 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 3 – 73 | PAS 1PROSITE-ProRule annotationAdd BLAST | 71 | |
Domaini | 77 – 116 | PAC 1Add BLAST | 40 | |
Domaini | 140 – 214 | PAS 2PROSITE-ProRule annotationAdd BLAST | 75 | |
Domaini | 218 – 255 | PAC 2Add BLAST | 38 | |
Domaini | 265 – 335 | PAS 3PROSITE-ProRule annotationAdd BLAST | 71 | |
Domaini | 402 – 606 | Histidine kinasePROSITE-ProRule annotationAdd BLAST | 205 |
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | COG4191, Bacteria |
InParanoidi | P16497 |
OMAi | LVESYFY |
PhylomeDBi | P16497 |
Family and domain databases
CDDi | cd00082, HisKA, 1 hit cd00130, PAS, 3 hits |
Gene3Di | 3.30.565.10, 1 hit |
InterProi | View protein in InterPro IPR003594, HATPase_C IPR036890, HATPase_C_sf IPR005467, His_kinase_dom IPR003661, HisK_dim/P IPR036097, HisK_dim/P_sf IPR001610, PAC IPR000014, PAS IPR035965, PAS-like_dom_sf IPR013767, PAS_fold IPR013655, PAS_fold_3 IPR004358, Sig_transdc_His_kin-like_C |
Pfami | View protein in Pfam PF02518, HATPase_c, 1 hit PF00512, HisKA, 1 hit PF00989, PAS, 2 hits PF08447, PAS_3, 1 hit |
PRINTSi | PR00344, BCTRLSENSOR |
SMARTi | View protein in SMART SM00387, HATPase_c, 1 hit SM00388, HisKA, 1 hit SM00086, PAC, 2 hits SM00091, PAS, 3 hits |
SUPFAMi | SSF47384, SSF47384, 1 hit SSF55785, SSF55785, 3 hits SSF55874, SSF55874, 1 hit |
TIGRFAMsi | TIGR00229, sensory_box, 3 hits |
PROSITEi | View protein in PROSITE PS50109, HIS_KIN, 1 hit PS50112, PAS, 3 hits |
i Sequence
Sequence statusi: Complete.
P16497-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEQDTQHVKP LQTKTDIHAV LASNGRIIYI SANSKLHLGY LQGEMIGSFL
60 70 80 90 100
KTFLHEEDQF LVESYFYNEH HLMPCTFRFI KKDHTIVWVE AAVEIVTTRA
110 120 130 140 150
ERTEREIILK MKVLEEETGH QSLNCEKHEI EPASPESTTY ITDDYERLVE
160 170 180 190 200
NLPSPLCISV KGKIVYVNSA MLSMLGAKSK DAIIGKSSYE FIEEEYHDIV
210 220 230 240 250
KNRIIRMQKG MEVGMIEQTW KRLDGTPVHL EVKASPTVYK NQQAELLLLI
260 270 280 290 300
DISSRKKFQT ILQKSRERYQ LLIQNSIDTI AVIHNGKWVF MNESGISLFE
310 320 330 340 350
AATYEDLIGK NIYDQLHPCD HEDVKERIQN IAEQKTESEI VKQSWFTFQN
360 370 380 390 400
RVIYTEMVCI PTTFFGEAAV QVILRDISER KQTEELMLKS EKLSIAGQLA
410 420 430 440 450
AGIAHEIRNP LTAIKGFLQL MKPTMEGNEH YFDIVFSELS RIELILSELL
460 470 480 490 500
MLAKPQQNAV KEYLNLKKLI GEVSALLETQ ANLNGIFIRT SYEKDSIYIN
510 520 530 540 550
GDQNQLKQVF INLIKNAVES MPDGGTVDII ITEDEHSVHV TVKDEGEGIP
560 570 580 590 600
EKVLNRIGEP FLTTKEKGTG LGLMVTFNII ENHQGVIHVD SHPEKGTAFK
ISFPKK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 276 | S → F in AAA22800 (PubMed:2104615).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M29450 Genomic DNA Translation: AAA22800.1 M31067 Genomic DNA Translation: AAA22664.1 AJ222587 Genomic DNA Translation: CAA10862.1 AL009126 Genomic DNA Translation: CAB13272.1 |
PIRi | A33496 |
RefSeqi | NP_389282.1, NC_000964.3 WP_003245779.1, NZ_JNCM01000035.1 |
Genome annotation databases
EnsemblBacteriai | CAB13272; CAB13272; BSU13990 |
GeneIDi | 939230 |
KEGGi | bsu:BSU13990 |
PATRICi | fig|224308.179.peg.1525 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M29450 Genomic DNA Translation: AAA22800.1 M31067 Genomic DNA Translation: AAA22664.1 AJ222587 Genomic DNA Translation: CAA10862.1 AL009126 Genomic DNA Translation: CAB13272.1 |
PIRi | A33496 |
RefSeqi | NP_389282.1, NC_000964.3 WP_003245779.1, NZ_JNCM01000035.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2VLG | X-ray | 1.70 | A/B/C/D | 10-117 | [»] | |
SASBDBi | P16497 | |||||
SMRi | P16497 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-58966N |
IntActi | P16497, 2 interactors |
STRINGi | 224308.BSU13990 |
Chemistry databases
BindingDBi | P16497 |
ChEMBLi | CHEMBL2111331 |
Proteomic databases
PaxDbi | P16497 |
PRIDEi | P16497 |
Genome annotation databases
EnsemblBacteriai | CAB13272; CAB13272; BSU13990 |
GeneIDi | 939230 |
KEGGi | bsu:BSU13990 |
PATRICi | fig|224308.179.peg.1525 |
Phylogenomic databases
eggNOGi | COG4191, Bacteria |
InParanoidi | P16497 |
OMAi | LVESYFY |
PhylomeDBi | P16497 |
Enzyme and pathway databases
BioCyci | BSUB:BSU13990-MONOMER |
BRENDAi | 2.7.13.3, 658 |
Miscellaneous databases
EvolutionaryTracei | P16497 |
PROi | PR:P16497 |
Family and domain databases
CDDi | cd00082, HisKA, 1 hit cd00130, PAS, 3 hits |
Gene3Di | 3.30.565.10, 1 hit |
InterProi | View protein in InterPro IPR003594, HATPase_C IPR036890, HATPase_C_sf IPR005467, His_kinase_dom IPR003661, HisK_dim/P IPR036097, HisK_dim/P_sf IPR001610, PAC IPR000014, PAS IPR035965, PAS-like_dom_sf IPR013767, PAS_fold IPR013655, PAS_fold_3 IPR004358, Sig_transdc_His_kin-like_C |
Pfami | View protein in Pfam PF02518, HATPase_c, 1 hit PF00512, HisKA, 1 hit PF00989, PAS, 2 hits PF08447, PAS_3, 1 hit |
PRINTSi | PR00344, BCTRLSENSOR |
SMARTi | View protein in SMART SM00387, HATPase_c, 1 hit SM00388, HisKA, 1 hit SM00086, PAC, 2 hits SM00091, PAS, 3 hits |
SUPFAMi | SSF47384, SSF47384, 1 hit SSF55785, SSF55785, 3 hits SSF55874, SSF55874, 1 hit |
TIGRFAMsi | TIGR00229, sensory_box, 3 hits |
PROSITEi | View protein in PROSITE PS50109, HIS_KIN, 1 hit PS50112, PAS, 3 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KINA_BACSU | |
Accessioni | P16497Primary (citable) accession number: P16497 Secondary accession number(s): P22863 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1990 |
Last sequence update: | July 15, 1998 | |
Last modified: | December 2, 2020 | |
This is version 165 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references