UniProtKB - P16456 (SELD_ECOLI)
Protein
Selenide, water dikinase
Gene
selD
Organism
Escherichia coli (strain K12)
Status
Functioni
Synthesizes selenophosphate from selenide and ATP.UniRule annotation2 Publications
Catalytic activityi
- ATP + H2O + hydrogenselenide = AMP + 2 H+ + phosphate + selenophosphateUniRule annotation2 PublicationsEC:2.7.9.3UniRule annotation2 Publications
Cofactori
Mg2+UniRule annotation1 PublicationNote: Binds 1 Mg2+ ion per monomer.UniRule annotation1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 17 | UniRule annotation | 1 | |
Binding sitei | 20 | ATPUniRule annotationBy similarity | 1 | |
Sitei | 20 | Important for catalytic activityUniRule annotation1 Publication | 1 | |
Metal bindingi | 51 | MagnesiumUniRule annotationCombined sources1 Publication | 1 | |
Binding sitei | 68 | ATPUniRule annotationBy similarity | 1 | |
Metal bindingi | 91 | MagnesiumUniRule annotationCombined sources1 Publication | 1 | |
Binding sitei | 91 | ATPUniRule annotationBy similarity | 1 | |
Metal bindingi | 227 | MagnesiumUniRule annotationCombined sources1 Publication | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 48 – 50 | ATPUniRule annotationBy similarity | 3 | |
Nucleotide bindingi | 139 – 141 | ATP; shared with dimeric partnerUniRule annotationBy similarity | 3 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- identical protein binding Source: UniProtKB
- magnesium ion binding Source: EcoCyc
- selenide, water dikinase activity Source: UniProtKB
GO - Biological processi
- selenocysteine biosynthetic process Source: UniProtKB
- tRNA seleno-modification Source: EcoCyc
Keywordsi
Molecular function | Kinase, Transferase |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Selenium |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10943-MONOMER MetaCyc:EG10943-MONOMER |
BRENDAi | 2.7.9.3, 2026 |
Names & Taxonomyi
Protein namesi | Recommended name: Selenide, water dikinaseUniRule annotation (EC:2.7.9.3UniRule annotation2 Publications)Alternative name(s): Selenium donor proteinUniRule annotation Selenophosphate synthaseUniRule annotation |
Gene namesi | Name:selDUniRule annotation Synonyms:fdhB Ordered Locus Names:b1764, JW1753 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
GO - Cellular componenti
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 13 | H → N: No loss of activity. 1 Publication | 1 | |
Mutagenesisi | 17 | C → S: Complete loss of activity. 2 Publications | 1 | |
Mutagenesisi | 18 | G → V: Partial loss of activity. 1 Publication | 1 | |
Mutagenesisi | 19 | C → S: No loss of activity. 1 Publication | 1 | |
Mutagenesisi | 20 | K → Q: Complete loss of activity. 1 Publication | 1 | |
Mutagenesisi | 20 | K → R: Marked loss of activity. 1 Publication | 1 | |
Mutagenesisi | 51 | D → A: Complete loss of activity. 1 Publication | 1 | |
Mutagenesisi | 68 | D → A: Complete loss of activity. 1 Publication | 1 | |
Mutagenesisi | 87 | N → A: Complete loss of activity. 1 Publication | 1 | |
Mutagenesisi | 91 | D → A: Complete loss of activity. 1 Publication | 1 | |
Mutagenesisi | 227 | D → A: Complete loss of activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000127620 | 1 – 347 | Selenide, water dikinaseAdd BLAST | 347 |
Proteomic databases
jPOSTi | P16456 |
PaxDbi | P16456 |
PRIDEi | P16456 |
Interactioni
Subunit structurei
Homodimer.
UniRule annotation1 PublicationGO - Molecular functioni
- identical protein binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 4259138, 21 interactors |
DIPi | DIP-10849N |
IntActi | P16456, 11 interactors |
STRINGi | 511145.b1764 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P16456 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0709, Bacteria |
InParanoidi | P16456 |
PhylomeDBi | P16456 |
Family and domain databases
CDDi | cd02195, SelD, 1 hit |
Gene3Di | 3.30.1330.10, 1 hit 3.90.650.10, 1 hit |
HAMAPi | MF_00625, SelD, 1 hit |
InterProi | View protein in InterPro IPR010918, PurM-like_C_dom IPR036676, PurM-like_C_sf IPR016188, PurM-like_N IPR036921, PurM-like_N_sf IPR023061, SelD_I IPR004536, SPS/SelD |
PANTHERi | PTHR10256, PTHR10256, 1 hit |
Pfami | View protein in Pfam PF00586, AIRS, 1 hit PF02769, AIRS_C, 1 hit |
PIRSFi | PIRSF036407, Selenphspht_syn, 1 hit |
SUPFAMi | SSF55326, SSF55326, 1 hit SSF56042, SSF56042, 1 hit |
TIGRFAMsi | TIGR00476, selD, 1 hit |
i Sequence
Sequence statusi: Complete.
P16456-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSENSIRLTQ YSHGAGCGCK ISPKVLETIL HSEQAKFVDP NLLVGNETRD
60 70 80 90 100
DAAVYDLGNG TSVISTTDFF MPIVDNPFDF GRIAATNAIS DIFAMGGKPI
110 120 130 140 150
MAIAILGWPI NKLSPEIARE VTEGGRYACR QAGIALAGGH SIDAPEPIFG
160 170 180 190 200
LAVTGIVPTE RVKKNSTAQA GCKLFLTKPL GIGVLTTAEK KSLLKPEHQG
210 220 230 240 250
LATEVMCRMN IAGASFANIE GVKAMTDVTG FGLLGHLSEM CQGAGVQARV
260 270 280 290 300
DYEAIPKLPG VEEYIKLGAV PGGTERNFAS YGHLMGEMPR EVRDLLCDPQ
310 320 330 340
TSGGLLLAVM PEAENEVKAT AAEFGIELTA IGELVPARGG RAMVEIR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M30184 Genomic DNA Translation: AAA74748.1 U00096 Genomic DNA Translation: AAC74834.1 AP009048 Genomic DNA Translation: BAA15552.1 J05076 Genomic DNA Translation: AAA83922.1 |
PIRi | JW0033 |
RefSeqi | NP_416278.1, NC_000913.3 WP_001295485.1, NZ_SSZK01000001.1 |
Genome annotation databases
EnsemblBacteriai | AAC74834; AAC74834; b1764 BAA15552; BAA15552; BAA15552 |
GeneIDi | 45114337 946768 |
KEGGi | ecj:JW1753 eco:b1764 |
PATRICi | fig|1411691.4.peg.490 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M30184 Genomic DNA Translation: AAA74748.1 U00096 Genomic DNA Translation: AAC74834.1 AP009048 Genomic DNA Translation: BAA15552.1 J05076 Genomic DNA Translation: AAA83922.1 |
PIRi | JW0033 |
RefSeqi | NP_416278.1, NC_000913.3 WP_001295485.1, NZ_SSZK01000001.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3U0O | X-ray | 2.25 | A/B | 1-347 | [»] | |
SMRi | P16456 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259138, 21 interactors |
DIPi | DIP-10849N |
IntActi | P16456, 11 interactors |
STRINGi | 511145.b1764 |
Proteomic databases
jPOSTi | P16456 |
PaxDbi | P16456 |
PRIDEi | P16456 |
Protocols and materials databases
DNASUi | 946768 |
Genome annotation databases
EnsemblBacteriai | AAC74834; AAC74834; b1764 BAA15552; BAA15552; BAA15552 |
GeneIDi | 45114337 946768 |
KEGGi | ecj:JW1753 eco:b1764 |
PATRICi | fig|1411691.4.peg.490 |
Organism-specific databases
EchoBASEi | EB0936 |
Phylogenomic databases
eggNOGi | COG0709, Bacteria |
InParanoidi | P16456 |
PhylomeDBi | P16456 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10943-MONOMER MetaCyc:EG10943-MONOMER |
BRENDAi | 2.7.9.3, 2026 |
Miscellaneous databases
PROi | PR:P16456 |
Family and domain databases
CDDi | cd02195, SelD, 1 hit |
Gene3Di | 3.30.1330.10, 1 hit 3.90.650.10, 1 hit |
HAMAPi | MF_00625, SelD, 1 hit |
InterProi | View protein in InterPro IPR010918, PurM-like_C_dom IPR036676, PurM-like_C_sf IPR016188, PurM-like_N IPR036921, PurM-like_N_sf IPR023061, SelD_I IPR004536, SPS/SelD |
PANTHERi | PTHR10256, PTHR10256, 1 hit |
Pfami | View protein in Pfam PF00586, AIRS, 1 hit PF02769, AIRS_C, 1 hit |
PIRSFi | PIRSF036407, Selenphspht_syn, 1 hit |
SUPFAMi | SSF55326, SSF55326, 1 hit SSF56042, SSF56042, 1 hit |
TIGRFAMsi | TIGR00476, selD, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SELD_ECOLI | |
Accessioni | P16456Primary (citable) accession number: P16456 Secondary accession number(s): P78172 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1990 |
Last sequence update: | August 1, 1990 | |
Last modified: | December 2, 2020 | |
This is version 166 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families