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Entry version 134 (05 Jun 2019)
Sequence version 1 (01 Aug 1990)
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Protein

Formate hydrogenlyase subunit 4

Gene

hycD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionOxidoreductase

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
EcoCyc:HYCD-MONOMER
ECOL316407:JW2692-MONOMER
MetaCyc:HYCD-MONOMER

Protein family/group databases

Transport Classification Database

More...
TCDBi
3.D.1.9.2 the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Formate hydrogenlyase subunit 4
Short name:
FHL subunit 4
Alternative name(s):
Hydrogenase 3 component D
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:hycD
Synonyms:hevD
Ordered Locus Names:b2722, JW2692
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

Escherichia coli strain K12 genome database

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EcoGenei
EG10477 hycD

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular_location_section">'Subcellular location'</a> section describes the subcellular compartment where each non-membrane region of a membrane-spanning protein is found.<p><a href='/help/topo_dom' target='_top'>More...</a></p>Topological domaini1 – 2PeriplasmicSequence analysis2
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular_location_section">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei3 – 23HelicalSequence analysisAdd BLAST21
Topological domaini24 – 67CytoplasmicSequence analysisAdd BLAST44
Transmembranei68 – 88HelicalSequence analysisAdd BLAST21
Topological domaini89 – 93PeriplasmicSequence analysis5
Transmembranei94 – 114HelicalSequence analysisAdd BLAST21
Topological domaini115 – 131CytoplasmicSequence analysisAdd BLAST17
Transmembranei132 – 152HelicalSequence analysisAdd BLAST21
Topological domaini153 – 167PeriplasmicSequence analysisAdd BLAST15
Transmembranei168 – 188HelicalSequence analysisAdd BLAST21
Topological domaini189 – 221CytoplasmicSequence analysisAdd BLAST33
Transmembranei222 – 242HelicalSequence analysisAdd BLAST21
Topological domaini243 – 253PeriplasmicSequence analysisAdd BLAST11
Transmembranei254 – 274HelicalSequence analysisAdd BLAST21
Topological domaini275 – 284CytoplasmicSequence analysis10
Transmembranei285 – 305HelicalSequence analysisAdd BLAST21
Topological domaini306 – 307PeriplasmicSequence analysis2

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001175341 – 307Formate hydrogenlyase subunit 4Add BLAST307

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

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PaxDbi
P16430

PRoteomics IDEntifications database

More...
PRIDEi
P16430

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

FHL comprises of a formate dehydrogenase, unidentified electron carriers and a hydrogenase (isoenzyme 3). In this non-energy conserving pathway molecular hydrogen and carbodioxide from formate are released.

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...
BioGridi
4261429, 6 interactors

ComplexPortal: manually curated resource of macromolecular complexes

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ComplexPortali
CPX-317 Formate hydrogenlyase-H/Hydrogenase-3 complex

Protein interaction database and analysis system

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IntActi
P16430, 1 interactor

STRING: functional protein association networks

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STRINGi
511145.b2722

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the complex I subunit 1 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
ENOG4107TDF Bacteria
COG0650 LUCA

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000228277

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P16430

KEGG Orthology (KO)

More...
KOi
K15829

Database for complete collections of gene phylogenies

More...
PhylomeDBi
P16430

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR001694 NADH_UbQ_OxRdtase_su1/FPO
IPR018086 NADH_UbQ_OxRdtase_su1_CS

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00146 NADHdh, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS00667 COMPLEX1_ND1_1, 1 hit
PS00668 COMPLEX1_ND1_2, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P16430-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSVLYPLIQA LVLFAVAPLL SGITRVARAR LHNRRGPGVL QEYRDIIKLL
60 70 80 90 100
GRQSVGPDAS GWVFRLTPYV MVGVMLTIAT ALPVVTVGSP LPQLGDLITL
110 120 130 140 150
LYLFAIARFF FAISGLDTGS PFTAIGASRE AMLGVLVEPM LLLGLWVAAQ
160 170 180 190 200
VAGSTNISNI TDTVYHWPLS QSIPLVLALC ACAFATFIEM GKLPFDLAEA
210 220 230 240 250
EQELQEGPLS EYSGSGFGVM KWGISLKQLV VLQMFVGVFI PWGQMETFTA
260 270 280 290 300
GGLLLALVIA IVKLVVGVLV IALFENSMAR LRLDITPRIT WAGFGFAFLA

FVSLLAA
Length:307
Mass (Da):33,029
Last modified:August 1, 1990 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i2A3F410620E82AC9
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

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EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

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DDBJi
Links Updated
X17506 Genomic DNA Translation: CAA35549.1
U29579 Genomic DNA Translation: AAA69232.1
U00096 Genomic DNA Translation: AAC75764.1
AP009048 Genomic DNA Translation: BAE76799.1

Protein sequence database of the Protein Information Resource

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PIRi
S08622

NCBI Reference Sequences

More...
RefSeqi
NP_417202.1, NC_000913.3
WP_000115224.1, NZ_LN832404.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
AAC75764; AAC75764; b2722
BAE76799; BAE76799; BAE76799

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
948994

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
ecj:JW2692
eco:b2722

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|1411691.4.peg.4019

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17506 Genomic DNA Translation: CAA35549.1
U29579 Genomic DNA Translation: AAA69232.1
U00096 Genomic DNA Translation: AAC75764.1
AP009048 Genomic DNA Translation: BAE76799.1
PIRiS08622
RefSeqiNP_417202.1, NC_000913.3
WP_000115224.1, NZ_LN832404.1

3D structure databases

Database of comparative protein structure models

More...
ModBasei
Search...

SWISS-MODEL Interactive Workspace

More...
SWISS-MODEL-Workspacei
Submit a new modelling project...

Protein-protein interaction databases

BioGridi4261429, 6 interactors
ComplexPortaliCPX-317 Formate hydrogenlyase-H/Hydrogenase-3 complex
IntActiP16430, 1 interactor
STRINGi511145.b2722

Protein family/group databases

TCDBi3.D.1.9.2 the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family

Proteomic databases

PaxDbiP16430
PRIDEiP16430

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75764; AAC75764; b2722
BAE76799; BAE76799; BAE76799
GeneIDi948994
KEGGiecj:JW2692
eco:b2722
PATRICifig|1411691.4.peg.4019

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...
EchoBASEi
EB0472
EcoGeneiEG10477 hycD

Phylogenomic databases

eggNOGiENOG4107TDF Bacteria
COG0650 LUCA
HOGENOMiHOG000228277
InParanoidiP16430
KOiK15829
PhylomeDBiP16430

Enzyme and pathway databases

BioCyciEcoCyc:HYCD-MONOMER
ECOL316407:JW2692-MONOMER
MetaCyc:HYCD-MONOMER

Miscellaneous databases

Protein Ontology

More...
PROi
PR:P16430

Family and domain databases

InterProiView protein in InterPro
IPR001694 NADH_UbQ_OxRdtase_su1/FPO
IPR018086 NADH_UbQ_OxRdtase_su1_CS
PfamiView protein in Pfam
PF00146 NADHdh, 1 hit
PROSITEiView protein in PROSITE
PS00667 COMPLEX1_ND1_1, 1 hit
PS00668 COMPLEX1_ND1_2, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiHYCD_ECOLI
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P16430
Secondary accession number(s): Q2MAA7
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: June 5, 2019
This is version 134 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
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