UniProtKB - P16422 (EPCAM_HUMAN)
Epithelial cell adhesion molecule
EPCAM
Functioni
May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.
4 PublicationsGO - Molecular functioni
- cadherin binding involved in cell-cell adhesion Source: GO_Central
- protein-containing complex binding Source: UniProtKB
GO - Biological processi
- negative regulation of cell-cell adhesion mediated by cadherin Source: UniProtKB
- positive regulation of cell population proliferation Source: UniProtKB
- positive regulation of stem cell proliferation Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: UniProtKB
- signal transduction involved in regulation of gene expression Source: UniProtKB
- stem cell differentiation Source: UniProtKB
- ureteric bud development Source: Ensembl
Keywordsi
Molecular function | Tumor antigen |
Enzyme and pathway databases
BRENDAi | 2.4.1.37, 2681 2.4.1.40, 2681 |
PathwayCommonsi | P16422 |
Reactomei | R-HSA-202733, Cell surface interactions at the vascular wall |
SignaLinki | P16422 |
SIGNORi | P16422 |
Names & Taxonomyi
Protein namesi | Recommended name: Epithelial cell adhesion moleculeShort name: Ep-CAM Alternative name(s): Adenocarcinoma-associated antigen Cell surface glycoprotein Trop-1 Epithelial cell surface antigen Epithelial glycoprotein Short name: EGP Epithelial glycoprotein 314 Short name: EGP314 Short name: hEGP314 KS 1/4 antigen KSA Major gastrointestinal tumor-associated protein GA733-2 Tumor-associated calcium signal transducer 1 CD_antigen: CD326 |
Gene namesi | Name:EPCAM Synonyms:GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:11529, EPCAM |
MIMi | 185535, gene |
neXtProti | NX_P16422 |
VEuPathDBi | HostDB:ENSG00000119888 |
Subcellular locationi
Plasma membrane
- Lateral cell membrane 3 Publications; Single-pass type I membrane protein 1 Publication
Other locations
- tight junction 1 Publication
Note: Colocalizes with CLDN7 at the lateral cell membrane and tight junction.1 Publication
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Plasma Membrane
- apical plasma membrane Source: MGI
- basolateral plasma membrane Source: MGI
- lateral plasma membrane Source: UniProtKB
- plasma membrane Source: UniProtKB
Other locations
- bicellular tight junction Source: UniProtKB
- cell surface Source: Ensembl
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 24 – 265 | ExtracellularSequence analysisAdd BLAST | 242 | |
Transmembranei | 266 – 288 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 289 – 314 | CytoplasmicSequence analysisAdd BLAST | 26 |
Keywords - Cellular componenti
Cell junction, Cell membrane, Membrane, Tight junctionPathology & Biotechi
Involvement in diseasei
Diarrhea 5, with tufting enteropathy, congenital (DIAR5)1 Publication
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_063829 | 66 | C → Y in DIAR5. 1 PublicationCorresponds to variant dbSNP:rs267606785EnsemblClinVar. | 1 |
Hereditary non-polyposis colorectal cancer 8 (HNPCC8)1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 74 | N → A: Changed glycosylation pattern. Complete loss of glycosylation and substantial decrease in protein expression; when associated with A-111 and A-198. 1 Publication | 1 | |
Mutagenesisi | 111 | N → A: Changed glycosylation pattern. Complete loss of glycosylation and substantial decrease in protein expression; when associated with A-74 and A-198. 1 Publication | 1 | |
Mutagenesisi | 198 | N → A: Decreased glycosyation, reduced protein stability and significant decrease in protein expression. Complete loss of glycosylation and substantial decrease in protein expression; when associated with A-74 and A-111. 1 Publication | 1 |
Keywords - Diseasei
Disease variant, Hereditary nonpolyposis colorectal cancerOrganism-specific databases
DisGeNETi | 4072 |
GeneReviewsi | EPCAM |
MalaCardsi | EPCAM |
MIMi | 613217, phenotype 613244, phenotype |
OpenTargetsi | ENSG00000119888 |
Orphaneti | 92050, Congenital tufting enteropathy 144, Lynch syndrome |
PharmGKBi | PA35493 |
Miscellaneous databases
Pharosi | P16422, Tbio |
Chemistry databases
ChEMBLi | CHEMBL3580493 |
DrugBanki | DB06607, Catumaxomab DB11075, Hypromellose DB05831, ING-1 DB05319, Oportuzumab monatox DB09336, Technetium Tc-99m nofetumomab merpentan |
Genetic variation databases
BioMutai | EPCAM |
DMDMi | 160266056 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 23 | Sequence analysisAdd BLAST | 23 | |
ChainiPRO_0000022467 | 24 – 314 | Epithelial cell adhesion moleculeAdd BLAST | 291 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 27 ↔ 46 | PROSITE-ProRule annotation1 Publication | ||
Disulfide bondi | 29 ↔ 59 | PROSITE-ProRule annotation1 Publication | ||
Disulfide bondi | 38 ↔ 48 | PROSITE-ProRule annotation1 Publication | ||
Disulfide bondi | 66 ↔ 99 | PROSITE-ProRule annotation1 Publication | ||
Glycosylationi | 74 | N-linked (GlcNAc...) asparagine; partial2 Publications | 1 | |
Disulfide bondi | 110 ↔ 116 | PROSITE-ProRule annotation1 Publication | ||
Glycosylationi | 111 | N-linked (GlcNAc...) asparagine2 Publications | 1 | |
Disulfide bondi | 118 ↔ 135 | PROSITE-ProRule annotation1 Publication | ||
Glycosylationi | 198 | N-linked (GlcNAc...) asparagine2 Publications | 1 |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
CPTACi | CPTAC-502 CPTAC-503 |
EPDi | P16422 |
jPOSTi | P16422 |
MassIVEi | P16422 |
MaxQBi | P16422 |
PaxDbi | P16422 |
PeptideAtlasi | P16422 |
PRIDEi | P16422 |
ProteomicsDBi | 53359 |
PTM databases
GlyConnecti | 1217, 34 N-Linked glycans (3 sites) |
GlyGeni | P16422, 4 sites, 34 N-linked glycans (3 sites), 1 O-linked glycan (1 site) |
iPTMneti | P16422 |
MetOSitei | P16422 |
PhosphoSitePlusi | P16422 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000119888, Expressed in jejunal mucosa and 212 other tissues |
ExpressionAtlasi | P16422, baseline and differential |
Genevisiblei | P16422, HS |
Organism-specific databases
HPAi | ENSG00000119888, Tissue enhanced (intestine) |
Interactioni
Subunit structurei
Monomer.
Interacts with phosphorylated CLDN7.
2 PublicationsBinary interactionsi
P16422
With | #Exp. | IntAct |
---|---|---|
CALR [P27797] | 3 | EBI-1171184,EBI-1049597 |
CDH1 [P12830] | 3 | EBI-1171184,EBI-727477 |
CDK5R1 [Q15078] | 3 | EBI-1171184,EBI-746189 |
DLST [P36957] | 3 | EBI-1171184,EBI-351007 |
NEK7 [Q8TDX7] | 3 | EBI-1171184,EBI-1055945 |
GO - Molecular functioni
- cadherin binding involved in cell-cell adhesion Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 110250, 17 interactors |
CORUMi | P16422 |
IntActi | P16422, 11 interactors |
MINTi | P16422 |
STRINGi | 9606.ENSP00000263735 |
Miscellaneous databases
RNActi | P16422, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P16422 |
SMRi | P16422 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 63 – 135 | Thyroglobulin type-1PROSITE-ProRule annotationAdd BLAST | 73 |
Sequence similaritiesi
Keywords - Domaini
Repeat, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QVSU, Eukaryota |
GeneTreei | ENSGT00390000018245 |
HOGENOMi | CLU_075326_0_0_1 |
InParanoidi | P16422 |
OMAi | NGTTTCW |
OrthoDBi | 1017141at2759 |
PhylomeDBi | P16422 |
TreeFami | TF332767 |
Family and domain databases
CDDi | cd00191, TY, 1 hit |
Gene3Di | 4.10.800.10, 1 hit |
InterProi | View protein in InterPro IPR043406, EPCAM/Trop-2 IPR041630, EpCAM_N IPR000716, Thyroglobulin_1 IPR036857, Thyroglobulin_1_sf |
PANTHERi | PTHR14168, PTHR14168, 1 hit |
Pfami | View protein in Pfam PF18635, EpCAM_N, 1 hit PF00086, Thyroglobulin_1, 1 hit |
SMARTi | View protein in SMART SM00211, TY, 1 hit |
SUPFAMi | SSF57610, SSF57610, 1 hit |
PROSITEi | View protein in PROSITE PS00484, THYROGLOBULIN_1_1, 1 hit PS51162, THYROGLOBULIN_1_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MAPPQVLAFG LLLAAATATF AAAQEECVCE NYKLAVNCFV NNNRQCQCTS
60 70 80 90 100
VGAQNTVICS KLAAKCLVMK AEMNGSKLGR RAKPEGALQN NDGLYDPDCD
110 120 130 140 150
ESGLFKAKQC NGTSMCWCVN TAGVRRTDKD TEITCSERVR TYWIIIELKH
160 170 180 190 200
KAREKPYDSK SLRTALQKEI TTRYQLDPKF ITSILYENNV ITIDLVQNSS
210 220 230 240 250
QKTQNDVDIA DVAYYFEKDV KGESLFHSKK MDLTVNGEQL DLDPGQTLIY
260 270 280 290 300
YVDEKAPEFS MQGLKAGVIA VIVVVVIAVV AGIVVLVISR KKRMAKYEKA
310
EIKEMGEMHR ELNA
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketC9JKY3 | C9JKY3_HUMAN | Epithelial cell adhesion molecule | EPCAM | 199 | Annotation score: | ||
B5MCA4 | B5MCA4_HUMAN | Epithelial cell adhesion molecule | EPCAM | 342 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 277 | I → M in AAA36151 (PubMed:2463074).Curated | 1 | |
Sequence conflicti | 277 | I → M in CAA32870 (PubMed:2463074).Curated | 1 | |
Sequence conflicti | 277 | I → M in AAA59543 (PubMed:2469722).Curated | 1 | |
Sequence conflicti | 303 | K → R in CAG47055 (Ref. 6) Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_063829 | 66 | C → Y in DIAR5. 1 PublicationCorresponds to variant dbSNP:rs267606785EnsemblClinVar. | 1 | |
Natural variantiVAR_018329 | 115 | M → T4 PublicationsCorresponds to variant dbSNP:rs1126497EnsemblClinVar. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M32325 mRNA Translation: AAA36151.1 X14758 mRNA Translation: CAA32870.1 M26481 mRNA Translation: AAA59543.1 M32306 mRNA Translation: AAA35723.1 M33011 mRNA Translation: AAA35861.1 M93036 , M93029, M93030, M93031, M93032, M93033, M93034, M93035 Genomic DNA Translation: AAB00775.1 CR542259 mRNA Translation: CAG47055.1 CR542283 mRNA Translation: CAG47078.1 AC079775 Genomic DNA Translation: AAY15095.1 CH471053 Genomic DNA Translation: EAX00218.1 BC014785 mRNA Translation: AAH14785.1 |
CCDSi | CCDS1833.1 |
PIRi | B48149 |
RefSeqi | NP_002345.2, NM_002354.2 |
Genome annotation databases
Ensembli | ENST00000263735.9; ENSP00000263735.4; ENSG00000119888.11 |
GeneIDi | 4072 |
KEGGi | hsa:4072 |
MANE-Selecti | ENST00000263735.9; ENSP00000263735.4; NM_002354.3; NP_002345.2 |
UCSCi | uc002rvx.4, human |
Similar proteinsi
Cross-referencesi
Web resourcesi
Atlas of Genetics and Cytogenetics in Oncology and Haematology |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M32325 mRNA Translation: AAA36151.1 X14758 mRNA Translation: CAA32870.1 M26481 mRNA Translation: AAA59543.1 M32306 mRNA Translation: AAA35723.1 M33011 mRNA Translation: AAA35861.1 M93036 , M93029, M93030, M93031, M93032, M93033, M93034, M93035 Genomic DNA Translation: AAB00775.1 CR542259 mRNA Translation: CAG47055.1 CR542283 mRNA Translation: CAG47078.1 AC079775 Genomic DNA Translation: AAY15095.1 CH471053 Genomic DNA Translation: EAX00218.1 BC014785 mRNA Translation: AAH14785.1 |
CCDSi | CCDS1833.1 |
PIRi | B48149 |
RefSeqi | NP_002345.2, NM_002354.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4MZV | X-ray | 1.86 | A | 24-265 | [»] | |
6I07 | X-ray | 2.35 | C/D | 24-265 | [»] | |
AlphaFoldDBi | P16422 | |||||
SMRi | P16422 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 110250, 17 interactors |
CORUMi | P16422 |
IntActi | P16422, 11 interactors |
MINTi | P16422 |
STRINGi | 9606.ENSP00000263735 |
Chemistry databases
ChEMBLi | CHEMBL3580493 |
DrugBanki | DB06607, Catumaxomab DB11075, Hypromellose DB05831, ING-1 DB05319, Oportuzumab monatox DB09336, Technetium Tc-99m nofetumomab merpentan |
PTM databases
GlyConnecti | 1217, 34 N-Linked glycans (3 sites) |
GlyGeni | P16422, 4 sites, 34 N-linked glycans (3 sites), 1 O-linked glycan (1 site) |
iPTMneti | P16422 |
MetOSitei | P16422 |
PhosphoSitePlusi | P16422 |
Genetic variation databases
BioMutai | EPCAM |
DMDMi | 160266056 |
Proteomic databases
CPTACi | CPTAC-502 CPTAC-503 |
EPDi | P16422 |
jPOSTi | P16422 |
MassIVEi | P16422 |
MaxQBi | P16422 |
PaxDbi | P16422 |
PeptideAtlasi | P16422 |
PRIDEi | P16422 |
ProteomicsDBi | 53359 |
Protocols and materials databases
ABCDi | P16422, 18 sequenced antibodies |
Antibodypediai | 3539, 4580 antibodies from 59 providers |
DNASUi | 4072 |
Genome annotation databases
Ensembli | ENST00000263735.9; ENSP00000263735.4; ENSG00000119888.11 |
GeneIDi | 4072 |
KEGGi | hsa:4072 |
MANE-Selecti | ENST00000263735.9; ENSP00000263735.4; NM_002354.3; NP_002345.2 |
UCSCi | uc002rvx.4, human |
Organism-specific databases
CTDi | 4072 |
DisGeNETi | 4072 |
GeneCardsi | EPCAM |
GeneReviewsi | EPCAM |
HGNCi | HGNC:11529, EPCAM |
HPAi | ENSG00000119888, Tissue enhanced (intestine) |
MalaCardsi | EPCAM |
MIMi | 185535, gene 613217, phenotype 613244, phenotype |
neXtProti | NX_P16422 |
OpenTargetsi | ENSG00000119888 |
Orphaneti | 92050, Congenital tufting enteropathy 144, Lynch syndrome |
PharmGKBi | PA35493 |
VEuPathDBi | HostDB:ENSG00000119888 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QVSU, Eukaryota |
GeneTreei | ENSGT00390000018245 |
HOGENOMi | CLU_075326_0_0_1 |
InParanoidi | P16422 |
OMAi | NGTTTCW |
OrthoDBi | 1017141at2759 |
PhylomeDBi | P16422 |
TreeFami | TF332767 |
Enzyme and pathway databases
BRENDAi | 2.4.1.37, 2681 2.4.1.40, 2681 |
PathwayCommonsi | P16422 |
Reactomei | R-HSA-202733, Cell surface interactions at the vascular wall |
SignaLinki | P16422 |
SIGNORi | P16422 |
Miscellaneous databases
BioGRID-ORCSi | 4072, 34 hits in 1078 CRISPR screens |
ChiTaRSi | EPCAM, human |
GeneWikii | Epithelial_cell_adhesion_molecule |
GenomeRNAii | 4072 |
Pharosi | P16422, Tbio |
PROi | PR:P16422 |
RNActi | P16422, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000119888, Expressed in jejunal mucosa and 212 other tissues |
ExpressionAtlasi | P16422, baseline and differential |
Genevisiblei | P16422, HS |
Family and domain databases
CDDi | cd00191, TY, 1 hit |
Gene3Di | 4.10.800.10, 1 hit |
InterProi | View protein in InterPro IPR043406, EPCAM/Trop-2 IPR041630, EpCAM_N IPR000716, Thyroglobulin_1 IPR036857, Thyroglobulin_1_sf |
PANTHERi | PTHR14168, PTHR14168, 1 hit |
Pfami | View protein in Pfam PF18635, EpCAM_N, 1 hit PF00086, Thyroglobulin_1, 1 hit |
SMARTi | View protein in SMART SM00211, TY, 1 hit |
SUPFAMi | SSF57610, SSF57610, 1 hit |
PROSITEi | View protein in PROSITE PS00484, THYROGLOBULIN_1_1, 1 hit PS51162, THYROGLOBULIN_1_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | EPCAM_HUMAN | |
Accessioni | P16422Primary (citable) accession number: P16422 Secondary accession number(s): P18180 Q9UCD0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1990 |
Last sequence update: | November 13, 2007 | |
Last modified: | May 25, 2022 | |
This is version 205 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human cell differentiation molecules
CD nomenclature of surface proteins of human leucocytes and list of entries - Human chromosome 2
Human chromosome 2: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families