UniProtKB - P16401 (H15_HUMAN)
Protein
Histone H1.5
Gene
H1-5
Organism
Homo sapiens (Human)
Status
Functioni
Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).By similarity
GO - Molecular functioni
- chromatin DNA binding Source: UniProtKB
- double-stranded DNA binding Source: GO_Central
- histone deacetylase binding Source: UniProtKB
- nucleosomal DNA binding Source: GO_Central
- RNA binding Source: UniProtKB
GO - Biological processi
- chromatin organization Source: UniProtKB
- chromosome condensation Source: GO_Central
- establishment of protein localization to chromatin Source: UniProtKB
- negative regulation of chromatin silencing Source: GO_Central
- negative regulation of DNA recombination Source: GO_Central
- negative regulation of transcription by RNA polymerase II Source: UniProtKB
- nucleosome assembly Source: InterPro
- nucleosome positioning Source: GO_Central
- positive regulation of cell growth Source: UniProtKB
- positive regulation of histone H3-K9 methylation Source: UniProtKB
- protein stabilization Source: UniProtKB
Keywordsi
Molecular function | DNA-binding |
Enzyme and pathway databases
PathwayCommonsi | P16401 |
Reactomei | R-HSA-140342, Apoptosis induced DNA fragmentation R-HSA-2559584, Formation of Senescence-Associated Heterochromatin Foci (SAHF) |
SIGNORi | P16401 |
Names & Taxonomyi
Protein namesi | Recommended name: Histone H1.5Alternative name(s): Histone H1a Histone H1b Histone H1s-3 |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:4719, H1-5 |
MIMi | 142711, gene |
neXtProti | NX_P16401 |
VEuPathDBi | HostDB:ENSG00000184357.4 |
Subcellular locationi
Nucleus
- nucleus Source: UniProtKB
Other locations
- chromatin Source: UniProtKB
- heterochromatin Source: UniProtKB
- nucleosome Source: InterPro
Keywords - Cellular componenti
Chromosome, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 3009 |
OpenTargetsi | ENSG00000184357 |
PharmGKBi | PA29097 |
Miscellaneous databases
Pharosi | P16401, Tbio |
Genetic variation databases
BioMutai | HIST1H1B |
DMDMi | 19856407 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources2 Publications | |||
ChainiPRO_0000195909 | 2 – 226 | Histone H1.5Add BLAST | 225 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserine; partialCombined sources1 Publication | 1 | |
Modified residuei | 2 | PhosphoserineCombined sources | 1 | |
Modified residuei | 11 | Phosphothreonine; by GSK3Combined sources2 Publications | 1 | |
Modified residuei | 17 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 18 | PhosphoserineCombined sources1 Publication | 1 | |
Modified residuei | 27 | N6-methyllysine1 Publication | 1 | |
Modified residuei | 37 | N6-(beta-hydroxybutyryl)lysine; alternateBy similarity | 1 | |
Modified residuei | 37 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 39 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 49 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 55 | N6-(beta-hydroxybutyryl)lysineBy similarity | 1 | |
Modified residuei | 57 | CitrullineBy similarity | 1 | |
Modified residuei | 67 | N6-(beta-hydroxybutyryl)lysineBy similarity | 1 | |
Modified residuei | 78 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 88 | N6-(beta-hydroxybutyryl)lysineBy similarity | 1 | |
Modified residuei | 93 | N6-(beta-hydroxybutyryl)lysineBy similarity | 1 | |
Modified residuei | 109 | N6-(beta-hydroxybutyryl)lysineBy similarity | 1 | |
Modified residuei | 138 | PhosphothreonineCombined sources1 Publication | 1 | |
Modified residuei | 155 | Phosphothreonine1 Publication | 1 | |
Modified residuei | 168 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 173 | Phosphoserine1 Publication | 1 | |
Modified residuei | 189 | Phosphoserine1 Publication | 1 |
Post-translational modificationi
H1 histones are progressively phosphorylated during the cell cycle, becoming maximally phosphorylated during late G2 phase and M phase, and being dephosphorylated sharply thereafter (By similarity). Phosphorylated at Thr-11 by GSK3B during mitosis in prometaphase and dephosphorylated in telophase.By similarity2 Publications
Citrullination at Arg-57 (H1R54ci) by PADI4 takes place within the DNA-binding site of H1 and results in its displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance.By similarity
Keywords - PTMi
Acetylation, Citrullination, Hydroxylation, Methylation, PhosphoproteinProteomic databases
EPDi | P16401 |
jPOSTi | P16401 |
MassIVEi | P16401 |
MaxQBi | P16401 |
PaxDbi | P16401 |
PeptideAtlasi | P16401 |
PRIDEi | P16401 |
ProteomicsDBi | 53351 |
TopDownProteomicsi | P16401 |
PTM databases
iPTMneti | P16401 |
PhosphoSitePlusi | P16401 |
SwissPalmi | P16401 |
Expressioni
Tissue specificityi
Ubiquitous. Expressed in the majority of the cell lines tested and in testis.1 Publication
Gene expression databases
Bgeei | ENSG00000184357, Expressed in stomach and 82 other tissues |
Genevisiblei | P16401, HS |
Organism-specific databases
HPAi | ENSG00000184357, Tissue enriched (lymphoid) |
Interactioni
Binary interactionsi
P16401
With | #Exp. | IntAct |
---|---|---|
CBX8 [Q9HC52] | 2 | EBI-5327611,EBI-712912 |
GO - Molecular functioni
- histone deacetylase binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 109264, 273 interactors |
IntActi | P16401, 210 interactors |
MINTi | P16401 |
STRINGi | 9606.ENSP00000330074 |
Miscellaneous databases
RNActi | P16401, protein |
Structurei
3D structure databases
SMRi | P16401 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P16401 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 39 – 112 | H15PROSITE-ProRule annotationAdd BLAST | 74 |
Domaini
The C-terminal domain is required for high-affinity binding to chromatin.By similarity
Sequence similaritiesi
Belongs to the histone H1/H5 family.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | KOG4012, Eukaryota |
GeneTreei | ENSGT00940000162950 |
HOGENOMi | CLU_052897_7_0_1 |
InParanoidi | P16401 |
OMAi | YLFNSAI |
OrthoDBi | 1565299at2759 |
PhylomeDBi | P16401 |
TreeFami | TF313664 |
Family and domain databases
CDDi | cd00073, H15, 1 hit |
Gene3Di | 1.10.10.10, 1 hit |
IDEALi | IID00028 |
InterProi | View protein in InterPro IPR005819, H1/H5 IPR005818, Histone_H1/H5_H15 IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF00538, Linker_histone, 1 hit |
PRINTSi | PR00624, HISTONEH5 |
SMARTi | View protein in SMART SM00526, H15, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit |
PROSITEi | View protein in PROSITE PS51504, H15, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P16401-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSETAPAETA TPAPVEKSPA KKKATKKAAG AGAAKRKATG PPVSELITKA
60 70 80 90 100
VAASKERNGL SLAALKKALA AGGYDVEKNN SRIKLGLKSL VSKGTLVQTK
110 120 130 140 150
GTGASGSFKL NKKAASGEAK PKAKKAGAAK AKKPAGATPK KAKKAAGAKK
160 170 180 190 200
AVKKTPKKAK KPAAAGVKKV AKSPKKAKAA AKPKKATKSP AKPKAVKPKA
210 220
AKPKAAKPKA AKPKAAKAKK AAAKKK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 216 – 218 | Missing AA sequence (PubMed:2613692).Curated | 3 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_036204 | 86 | G → D in a colorectal cancer sample; somatic mutation. 1 Publication | 1 | |
Natural variantiVAR_049308 | 144 | K → R. Corresponds to variant dbSNP:rs11970638Ensembl. | 1 | |
Natural variantiVAR_049309 | 211 | A → T. Corresponds to variant dbSNP:rs34144478Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X83509 Genomic DNA Translation: CAA58498.1 AF531304 Genomic DNA Translation: AAN06704.1 Z98744 Genomic DNA No translation available. BC069101 mRNA Translation: AAH69101.1 BC101581 mRNA Translation: AAI01582.1 BC101583 mRNA Translation: AAI01584.1 |
CCDSi | CCDS4635.1 |
PIRi | S51660 |
RefSeqi | NP_005313.1, NM_005322.2 |
Genome annotation databases
Ensembli | ENST00000331442; ENSP00000330074; ENSG00000184357 |
GeneIDi | 3009 |
KEGGi | hsa:3009 |
UCSCi | uc003njx.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X83509 Genomic DNA Translation: CAA58498.1 AF531304 Genomic DNA Translation: AAN06704.1 Z98744 Genomic DNA No translation available. BC069101 mRNA Translation: AAH69101.1 BC101581 mRNA Translation: AAI01582.1 BC101583 mRNA Translation: AAI01584.1 |
CCDSi | CCDS4635.1 |
PIRi | S51660 |
RefSeqi | NP_005313.1, NM_005322.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2FE2 | model | - | A | 2-226 | [»] | |
2RHI | X-ray | 1.66 | B | 23-27 | [»] | |
SMRi | P16401 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 109264, 273 interactors |
IntActi | P16401, 210 interactors |
MINTi | P16401 |
STRINGi | 9606.ENSP00000330074 |
PTM databases
iPTMneti | P16401 |
PhosphoSitePlusi | P16401 |
SwissPalmi | P16401 |
Genetic variation databases
BioMutai | HIST1H1B |
DMDMi | 19856407 |
Proteomic databases
EPDi | P16401 |
jPOSTi | P16401 |
MassIVEi | P16401 |
MaxQBi | P16401 |
PaxDbi | P16401 |
PeptideAtlasi | P16401 |
PRIDEi | P16401 |
ProteomicsDBi | 53351 |
TopDownProteomicsi | P16401 |
Protocols and materials databases
Antibodypediai | 54587, 196 antibodies |
DNASUi | 3009 |
Genome annotation databases
Ensembli | ENST00000331442; ENSP00000330074; ENSG00000184357 |
GeneIDi | 3009 |
KEGGi | hsa:3009 |
UCSCi | uc003njx.4, human |
Organism-specific databases
CTDi | 3009 |
DisGeNETi | 3009 |
GeneCardsi | H1-5 |
HGNCi | HGNC:4719, H1-5 |
HPAi | ENSG00000184357, Tissue enriched (lymphoid) |
MIMi | 142711, gene |
neXtProti | NX_P16401 |
OpenTargetsi | ENSG00000184357 |
PharmGKBi | PA29097 |
VEuPathDBi | HostDB:ENSG00000184357.4 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4012, Eukaryota |
GeneTreei | ENSGT00940000162950 |
HOGENOMi | CLU_052897_7_0_1 |
InParanoidi | P16401 |
OMAi | YLFNSAI |
OrthoDBi | 1565299at2759 |
PhylomeDBi | P16401 |
TreeFami | TF313664 |
Enzyme and pathway databases
PathwayCommonsi | P16401 |
Reactomei | R-HSA-140342, Apoptosis induced DNA fragmentation R-HSA-2559584, Formation of Senescence-Associated Heterochromatin Foci (SAHF) |
SIGNORi | P16401 |
Miscellaneous databases
BioGRID-ORCSi | 3009, 15 hits in 990 CRISPR screens |
EvolutionaryTracei | P16401 |
GeneWikii | HIST1H1B |
GenomeRNAii | 3009 |
Pharosi | P16401, Tbio |
PROi | PR:P16401 |
RNActi | P16401, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000184357, Expressed in stomach and 82 other tissues |
Genevisiblei | P16401, HS |
Family and domain databases
CDDi | cd00073, H15, 1 hit |
Gene3Di | 1.10.10.10, 1 hit |
IDEALi | IID00028 |
InterProi | View protein in InterPro IPR005819, H1/H5 IPR005818, Histone_H1/H5_H15 IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF00538, Linker_histone, 1 hit |
PRINTSi | PR00624, HISTONEH5 |
SMARTi | View protein in SMART SM00526, H15, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit |
PROSITEi | View protein in PROSITE PS51504, H15, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | H15_HUMAN | |
Accessioni | P16401Primary (citable) accession number: P16401 Secondary accession number(s): Q14529, Q3MJ42 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1990 |
Last sequence update: | January 23, 2007 | |
Last modified: | April 7, 2021 | |
This is version 191 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 6
Human chromosome 6: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families