UniProtKB - P16387 (ODPA_YEAST)
Pyruvate dehydrogenase E1 component subunit alpha, mitochondrial
PDA1
Functioni
The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2.
Miscellaneous
Catalytic activityi
- (R)-N6-lipoyl-L-lysyl-[dihydrolipoyllysine-residue acetyltransferase] + H+ + pyruvate = (R)-N6-(S8-acetyldihydrolipoyl)-L-lysyl-[dihydrolipoyllysine-residue acetyltransferase] + CO2EC:1.2.4.1
Cofactori
Activity regulationi
GO - Molecular functioni
GO - Biological processi
- acetyl-CoA biosynthetic process from pyruvate Source: SGD
- glycolytic process Source: UniProtKB-KW
- pseudohyphal growth Source: SGD
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Glycolysis |
Ligand | Pyruvate, Thiamine pyrophosphate |
Enzyme and pathway databases
Reactomei | R-SCE-389661, Glyoxylate metabolism and glycine degradation R-SCE-70268, Pyruvate metabolism |
Names & Taxonomyi
Protein namesi | Recommended name: Pyruvate dehydrogenase E1 component subunit alpha, mitochondrial (EC:1.2.4.1)Alternative name(s): Pyruvate dehydrogenase complex component E1 alpha Short name: PDHE1-A |
Gene namesi | Name:PDA1 Ordered Locus Names:YER178W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000000980, PDA1 |
VEuPathDBi | FungiDB:YER178W |
Subcellular locationi
Mitochondrion
Mitochondrion
- mitochondrial nucleoid Source: SGD
- mitochondrial pyruvate dehydrogenase complex Source: SGD
- mitochondrion Source: SGD
Keywords - Cellular componenti
MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 33 | Mitochondrion1 PublicationAdd BLAST | 33 | |
ChainiPRO_0000020452 | 34 – 420 | Pyruvate dehydrogenase E1 component subunit alpha, mitochondrialAdd BLAST | 387 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 313 | Phosphoserine; by PDK1 and PDK2Combined sources1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P16387 |
PaxDbi | P16387 |
PRIDEi | P16387 |
PTM databases
iPTMneti | P16387 |
Interactioni
Subunit structurei
Pyruvate dehydrogenase (E1) is a tetramer of 2 alpha and 2 beta subunits. Eukaryotic pyruvate dehydrogenase (PDH) complexes are organized as a core consisting of the oligomeric dihydrolipoamide acetyl-transferase (E2), around which are arranged multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide dehydrogenase (E3) and protein X (E3BP) bound by non-covalent bonds.
Protein-protein interaction databases
BioGRIDi | 36931, 502 interactors |
ComplexPortali | CPX-3207, Mitochondrial pyruvate dehydrogenase complex |
DIPi | DIP-5117N |
IntActi | P16387, 37 interactors |
MINTi | P16387 |
STRINGi | 4932.YER178W |
Miscellaneous databases
RNActi | P16387, protein |
Family & Domainsi
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG0225, Eukaryota |
GeneTreei | ENSGT00530000063174 |
HOGENOMi | CLU_029393_5_2_1 |
InParanoidi | P16387 |
OMAi | LGYEMPC |
Family and domain databases
InterProi | View protein in InterPro IPR001017, DH_E1 IPR017597, Pyrv_DH_E1_asu_subgrp-y IPR029061, THDP-binding |
Pfami | View protein in Pfam PF00676, E1_dh, 1 hit |
SUPFAMi | SSF52518, SSF52518, 1 hit |
TIGRFAMsi | TIGR03182, PDH_E1_alph_y, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MLAASFKRQP SQLVRGLGAV LRTPTRIGHV RTMATLKTTD KKAPEDIEGS
60 70 80 90 100
DTVQIELPES SFESYMLEPP DLSYETSKAT LLQMYKDMVI IRRMEMACDA
110 120 130 140 150
LYKAKKIRGF CHLSVGQEAI AVGIENAITK LDSIITSYRC HGFTFMRGAS
160 170 180 190 200
VKAVLAELMG RRAGVSYGKG GSMHLYAPGF YGGNGIVGAQ VPLGAGLAFA
210 220 230 240 250
HQYKNEDACS FTLYGDGASN QGQVFESFNM AKLWNLPVVF CCENNKYGMG
260 270 280 290 300
TAASRSSAMT EYFKRGQYIP GLKVNGMDIL AVYQASKFAK DWCLSGKGPL
310 320 330 340 350
VLEYETYRYG GHSMSDPGTT YRTRDEIQHM RSKNDPIAGL KMHLIDLGIA
360 370 380 390 400
TEAEVKAYDK SARKYVDEQV ELADAAPPPE AKLSILFEDV YVKGTETPTL
410 420
RGRIPEDTWD FKKQGFASRD
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 76 | T → S in AAA34847 (PubMed:2684159).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M29582 mRNA Translation: AAA34847.1 X71664 Genomic DNA Translation: CAA50657.1 U18922 Genomic DNA Translation: AAB64705.1 Different initiation. M87549 Genomic DNA Translation: AAA34572.1 D10865 Genomic DNA Translation: BAA01636.1 BK006939 Genomic DNA Translation: DAA07841.1 |
PIRi | A36743, DEBYPA |
RefSeqi | NP_011105.4, NM_001179068.3 |
Genome annotation databases
EnsemblFungii | YER178W_mRNA; YER178W; YER178W |
GeneIDi | 856925 |
KEGGi | sce:YER178W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M29582 mRNA Translation: AAA34847.1 X71664 Genomic DNA Translation: CAA50657.1 U18922 Genomic DNA Translation: AAB64705.1 Different initiation. M87549 Genomic DNA Translation: AAA34572.1 D10865 Genomic DNA Translation: BAA01636.1 BK006939 Genomic DNA Translation: DAA07841.1 |
PIRi | A36743, DEBYPA |
RefSeqi | NP_011105.4, NM_001179068.3 |
3D structure databases
AlphaFoldDBi | P16387 |
SMRi | P16387 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 36931, 502 interactors |
ComplexPortali | CPX-3207, Mitochondrial pyruvate dehydrogenase complex |
DIPi | DIP-5117N |
IntActi | P16387, 37 interactors |
MINTi | P16387 |
STRINGi | 4932.YER178W |
PTM databases
iPTMneti | P16387 |
Proteomic databases
MaxQBi | P16387 |
PaxDbi | P16387 |
PRIDEi | P16387 |
Genome annotation databases
EnsemblFungii | YER178W_mRNA; YER178W; YER178W |
GeneIDi | 856925 |
KEGGi | sce:YER178W |
Organism-specific databases
SGDi | S000000980, PDA1 |
VEuPathDBi | FungiDB:YER178W |
Phylogenomic databases
eggNOGi | KOG0225, Eukaryota |
GeneTreei | ENSGT00530000063174 |
HOGENOMi | CLU_029393_5_2_1 |
InParanoidi | P16387 |
OMAi | LGYEMPC |
Enzyme and pathway databases
Reactomei | R-SCE-389661, Glyoxylate metabolism and glycine degradation R-SCE-70268, Pyruvate metabolism |
Miscellaneous databases
PROi | PR:P16387 |
RNActi | P16387, protein |
Family and domain databases
InterProi | View protein in InterPro IPR001017, DH_E1 IPR017597, Pyrv_DH_E1_asu_subgrp-y IPR029061, THDP-binding |
Pfami | View protein in Pfam PF00676, E1_dh, 1 hit |
SUPFAMi | SSF52518, SSF52518, 1 hit |
TIGRFAMsi | TIGR03182, PDH_E1_alph_y, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ODPA_YEAST | |
Accessioni | P16387Primary (citable) accession number: P16387 Secondary accession number(s): D3DM87 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1990 |
Last sequence update: | February 1, 1995 | |
Last modified: | May 25, 2022 | |
This is version 214 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome V
Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names