UniProtKB - P16378 (GNAO_DROME)
Protein
G protein alpha o subunit
Gene
Galphao
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Plays a role in glial cell differentiation during embryogenesis; loco, Galphai and the G-protein coupled receptor, moody, are required in the surface glia to achieve effective insulation of the nerve cord.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 47 | MagnesiumBy similarity | 1 | |
Metal bindingi | 182 | MagnesiumBy similarity | 1 | |
Binding sitei | 326 | GTP; via amide nitrogenBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 40 – 47 | GTPBy similarity | 8 | |
Nucleotide bindingi | 176 – 182 | GTPBy similarity | 7 | |
Nucleotide bindingi | 201 – 205 | GTPBy similarity | 5 | |
Nucleotide bindingi | 270 – 273 | GTPBy similarity | 4 |
GO - Molecular functioni
- G-protein beta/gamma-subunit complex binding Source: GO_Central
- G protein-coupled receptor binding Source: GO_Central
- GTPase activity Source: GO_Central
- GTP binding Source: FlyBase
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- adenylate cyclase-modulating G protein-coupled receptor signaling pathway Source: GO_Central
- asymmetric cell division Source: FlyBase
- axon ensheathment in central nervous system Source: UniProtKB
- behavioral response to starvation Source: FlyBase
- calcium-mediated signaling Source: FlyBase
- cell adhesion involved in heart morphogenesis Source: FlyBase
- cortical actin cytoskeleton organization Source: UniProtKB
- establishment of glial blood-brain barrier Source: FlyBase
- establishment of imaginal disc-derived wing hair orientation Source: FlyBase
- G protein-coupled receptor signaling pathway Source: UniProtKB
- heart development Source: FlyBase
- negative regulation of synaptic growth at neuromuscular junction Source: FlyBase
- non-canonical Wnt signaling pathway Source: FlyBase
- sensory perception of sweet taste Source: FlyBase
- septate junction assembly Source: FlyBase
- ventral cord development Source: FlyBase
Keywordsi
Molecular function | Transducer |
Ligand | GTP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-DME-112043, PLC beta mediated events R-DME-202040, G-protein activation R-DME-4086398, Ca2+ pathway R-DME-6814122, Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding |
Names & Taxonomyi
Protein namesi | Recommended name: G protein alpha o subunitAlternative name(s): Guanine nucleotide-binding protein G(o) subunit alpha 47A |
Gene namesi | Name:Galphao Synonyms:G-oa47A, G-oalpha47A ORF Names:CG2204 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0001122, Galphao |
Subcellular locationi
Plasma Membrane
- heterotrimeric G-protein complex Source: FlyBase
- plasma membrane Source: FlyBase
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000203716 | 2 – 354 | G protein alpha o subunitAdd BLAST | 353 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 2 | N-myristoyl glycineSequence analysis | 1 | |
Lipidationi | 3 | S-palmitoyl cysteineSequence analysis | 1 |
Keywords - PTMi
Lipoprotein, Myristate, PalmitateProteomic databases
PaxDbi | P16378 |
PRIDEi | P16378 |
Expressioni
Tissue specificityi
Expressed primarily in neuronal cell bodies in the brain, optic lobe, and thoracic and abdominal ganglia. Also expressed in antenna, oocytes and ovarian nurse cells.4 Publications
Developmental stagei
Expressed in the surface glial cells of the nerve cords at the larval stage (at protein level). Expressed throughout development.2 Publications
Gene expression databases
Bgeei | FBgn0001122, Expressed in brain and 52 other tissues |
ExpressionAtlasi | P16378, baseline and differential |
Genevisiblei | P16378, DM |
Interactioni
Subunit structurei
G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.
Binary interactionsi
P16378
With | #Exp. | IntAct |
---|---|---|
pins [Q9VB22] | 2 | EBI-197464,EBI-116643 |
GO - Molecular functioni
- G protein-coupled receptor binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 61922, 29 interactors |
DIPi | DIP-17224N |
IntActi | P16378, 6 interactors |
STRINGi | 7227.FBpp0087359 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 32 – 354 | G-alphaPROSITE-ProRule annotationAdd BLAST | 323 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 35 – 48 | G1 motifPROSITE-ProRule annotationAdd BLAST | 14 | |
Regioni | 174 – 182 | G2 motifPROSITE-ProRule annotation | 9 | |
Regioni | 197 – 206 | G3 motifPROSITE-ProRule annotation | 10 | |
Regioni | 266 – 273 | G4 motifPROSITE-ProRule annotation | 8 | |
Regioni | 324 – 329 | G5 motifPROSITE-ProRule annotation | 6 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0082, Eukaryota |
GeneTreei | ENSGT00940000155883 |
HOGENOMi | CLU_014184_6_0_1 |
InParanoidi | P16378 |
OMAi | MDVVARM |
PhylomeDBi | P16378 |
Family and domain databases
CDDi | cd00066, G-alpha, 1 hit |
Gene3Di | 1.10.400.10, 1 hit |
InterProi | View protein in InterPro IPR001408, Gprotein_alpha_I IPR001019, Gprotein_alpha_su IPR011025, GproteinA_insert IPR027417, P-loop_NTPase |
PANTHERi | PTHR10218, PTHR10218, 1 hit |
Pfami | View protein in Pfam PF00503, G-alpha, 1 hit |
PRINTSi | PR00318, GPROTEINA PR00441, GPROTEINAI |
SMARTi | View protein in SMART SM00275, G_alpha, 1 hit |
SUPFAMi | SSF47895, SSF47895, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51882, G_ALPHA, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform A (identifier: P16378-1) [UniParc]FASTAAdd to basket
Also known as: I
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGCAQSAEER AAAARSRLIE RNLKEDGIQA AKDIKLLLLG AGESGKSTIV
60 70 80 90 100
KQMKIIHESG FTAEDFKQYR PVVYSNTIQS LVAILRAMPT LSIQYSNNER
110 120 130 140 150
ESDAKMVFDV CQRMHDTEPF SEELLAAMKR LWQDAGVQEC FSRSNEYQLN
160 170 180 190 200
DSAKYFLDDL DRLGAKDYQP TEQDILRTRV KTTGIVEVHF SFKNLNFKLF
210 220 230 240 250
DVGGQRSERK KWIHCFEDVT AIIFCVAMSE YDQVLHEDET TNRMQESLKL
260 270 280 290 300
FDSICNNKWF TDTSIILFLN KKDLFEEKIR KSPLTICFPE YTGGQEYGEA
310 320 330 340 350
AAYIQAQFEA KNKSTSKEIY CHMTCATDTN NIQFVFDAVT DVIIANNLRG
CGLY
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketD5AEN0 | D5AEN0_DROME | G protein alpha o subunit, isoform ... | Galphao alpha(o), Bkh, bkh, DG[[o]], DGalpha0 | 127 | Annotation score: |
Sequence cautioni
The sequence AAN71523 differs from that shown. Reason: Erroneous termination. Truncated C-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 88 | M → I in AAA28585 (PubMed:2509463).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_001830 | 4 – 21 | AQSAE…RLIER → TTSAEERAAIQRSKQIEK in isoform B. 4 PublicationsAdd BLAST | 18 |
Sequence databases
Genome annotation databases
EnsemblMetazoai | FBtr0088264; FBpp0087359; FBgn0001122 [P16378-2] FBtr0088265; FBpp0087360; FBgn0001122 [P16378-1] FBtr0088266; FBpp0087361; FBgn0001122 [P16378-1] FBtr0088267; FBpp0087362; FBgn0001122 [P16378-2] FBtr0088268; FBpp0087363; FBgn0001122 [P16378-2] FBtr0088269; FBpp0087364; FBgn0001122 [P16378-2] FBtr0088270; FBpp0087365; FBgn0001122 [P16378-2] FBtr0088271; FBpp0089315; FBgn0001122 [P16378-2] FBtr0088272; FBpp0089316; FBgn0001122 [P16378-1] |
GeneIDi | 36104 |
KEGGi | dme:Dmel_CG2204 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
3D structure databases
SMRi | P16378 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 61922, 29 interactors |
DIPi | DIP-17224N |
IntActi | P16378, 6 interactors |
STRINGi | 7227.FBpp0087359 |
Proteomic databases
PaxDbi | P16378 |
PRIDEi | P16378 |
Genome annotation databases
EnsemblMetazoai | FBtr0088264; FBpp0087359; FBgn0001122 [P16378-2] FBtr0088265; FBpp0087360; FBgn0001122 [P16378-1] FBtr0088266; FBpp0087361; FBgn0001122 [P16378-1] FBtr0088267; FBpp0087362; FBgn0001122 [P16378-2] FBtr0088268; FBpp0087363; FBgn0001122 [P16378-2] FBtr0088269; FBpp0087364; FBgn0001122 [P16378-2] FBtr0088270; FBpp0087365; FBgn0001122 [P16378-2] FBtr0088271; FBpp0089315; FBgn0001122 [P16378-2] FBtr0088272; FBpp0089316; FBgn0001122 [P16378-1] |
GeneIDi | 36104 |
KEGGi | dme:Dmel_CG2204 |
Organism-specific databases
CTDi | 36104 |
FlyBasei | FBgn0001122, Galphao |
Phylogenomic databases
eggNOGi | KOG0082, Eukaryota |
GeneTreei | ENSGT00940000155883 |
HOGENOMi | CLU_014184_6_0_1 |
InParanoidi | P16378 |
OMAi | MDVVARM |
PhylomeDBi | P16378 |
Enzyme and pathway databases
Reactomei | R-DME-112043, PLC beta mediated events R-DME-202040, G-protein activation R-DME-4086398, Ca2+ pathway R-DME-6814122, Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding |
Miscellaneous databases
BioGRID-ORCSi | 36104, 0 hits in 3 CRISPR screens |
ChiTaRSi | Galphao, fly |
GenomeRNAii | 36104 |
PROi | PR:P16378 |
Gene expression databases
Bgeei | FBgn0001122, Expressed in brain and 52 other tissues |
ExpressionAtlasi | P16378, baseline and differential |
Genevisiblei | P16378, DM |
Family and domain databases
CDDi | cd00066, G-alpha, 1 hit |
Gene3Di | 1.10.400.10, 1 hit |
InterProi | View protein in InterPro IPR001408, Gprotein_alpha_I IPR001019, Gprotein_alpha_su IPR011025, GproteinA_insert IPR027417, P-loop_NTPase |
PANTHERi | PTHR10218, PTHR10218, 1 hit |
Pfami | View protein in Pfam PF00503, G-alpha, 1 hit |
PRINTSi | PR00318, GPROTEINA PR00441, GPROTEINAI |
SMARTi | View protein in SMART SM00275, G_alpha, 1 hit |
SUPFAMi | SSF47895, SSF47895, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51882, G_ALPHA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GNAO_DROME | |
Accessioni | P16378Primary (citable) accession number: P16378 Secondary accession number(s): A4UZB6 Q9V5L6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1990 |
Last sequence update: | August 1, 1990 | |
Last modified: | December 2, 2020 | |
This is version 196 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families