UniProtKB - P16371 (GROU_DROME)
Protein groucho
gro
Functioni
Transcriptional corepressor that regulates transcription when recruited to specific target DNA by hairy-related bHLH proteins. Maternally required for neurogenesis; in the segregation of the neuroectoderm. Directly or indirectly interacts with Notch and Delta.
1 PublicationGO - Molecular functioni
- CRD domain binding Source: UniProtKB
- DNA-binding transcription factor binding Source: FlyBase
- HMG box domain binding Source: UniProtKB
- transcription corepressor activity Source: UniProtKB
- transcription factor binding Source: FlyBase
GO - Biological processi
- cell differentiation Source: UniProtKB-KW
- epidermal growth factor receptor signaling pathway Source: FlyBase
- fibroblast growth factor receptor signaling pathway Source: FlyBase
- negative regulation of canonical Wnt signaling pathway Source: UniProtKB
- negative regulation of Toll signaling pathway Source: FlyBase
- negative regulation of transcription, DNA-templated Source: UniProtKB
- negative regulation of transcription by RNA polymerase II Source: FlyBase
- nervous system development Source: FlyBase
- regulation of transcription, DNA-templated Source: FlyBase
- sex determination, primary response to X:A ratio Source: FlyBase
- torso signaling pathway Source: FlyBase
- Wnt signaling pathway Source: UniProtKB-KW
Keywordsi
Molecular function | Developmental protein, Repressor |
Biological process | Differentiation, Neurogenesis, Transcription, Transcription regulation, Wnt signaling pathway |
Enzyme and pathway databases
Reactomei | R-DME-201722, Formation of the beta-catenin:TCF transactivating complex R-DME-209421, Transcription activation by ARM R-DME-209441, WG ligand not bound to FZ receptors R-DME-3769402, Deactivation of the beta-catenin transactivating complex R-DME-4641265, Repression of WNT target genes |
SignaLinki | P16371 |
Names & Taxonomyi
Protein namesi | Recommended name: Protein grouchoAlternative name(s): Enhancer of split m9/10 protein Short name: E(spl)m9/10 |
Gene namesi | Name:gro Synonyms:E(spl)m9/m10 ORF Names:CG8384 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0001139, gro |
VEuPathDBi | VectorBase:FBgn0001139 |
Subcellular locationi
Nucleus
Nucleus
- nucleoplasm Source: Reactome
- nucleus Source: GO_Central
Other locations
- protein-containing complex Source: FlyBase
- transcription regulator complex Source: GO_Central
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000051010 | 1 – 730 | Protein grouchoAdd BLAST | 730 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 205 | Phosphoserine1 Publication | 1 | |
Modified residuei | 207 | Phosphoserine1 Publication | 1 | |
Modified residuei | 218 | Phosphoserine1 Publication | 1 | |
Modified residuei | 242 | Phosphoserine; by CK2Sequence analysis | 1 | |
Modified residuei | 258 | Phosphoserine; by CDC2Sequence analysis | 1 | |
Modified residuei | 267 | Phosphoserine1 Publication | 1 | |
Modified residuei | 326 | Phosphothreonine1 Publication | 1 | |
Modified residuei | 328 | Phosphothreonine1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
Phosphoprotein, Ubl conjugationProteomic databases
PaxDbi | P16371 |
PTM databases
iPTMneti | P16371 |
Expressioni
Developmental stagei
Gene expression databases
Bgeei | FBgn0001139, Expressed in embryo and 29 other tissues |
ExpressionAtlasi | P16371, baseline and differential |
Interactioni
Subunit structurei
Forms a complex with the hairy/Enhancer of split/deadpan family of basic helix-loop-helix proteins in order to repress transcription. Its activity in regulating transcription depends on other proteins as it lacks a DNA-binding motif.
Binary interactionsi
P16371
Isoform A [P16371-2]
With | #Exp. | IntAct |
---|---|---|
h [P14003] | 2 | EBI-15661898,EBI-123011 |
Myc [Q9W4S7] | 3 | EBI-15661898,EBI-120162 |
GO - Molecular functioni
- CRD domain binding Source: UniProtKB
- DNA-binding transcription factor binding Source: FlyBase
- HMG box domain binding Source: UniProtKB
- transcription factor binding Source: FlyBase
Protein-protein interaction databases
BioGRIDi | 68062, 87 interactors |
DIPi | DIP-40N |
ELMi | P16371 |
IntActi | P16371, 39 interactors |
MINTi | P16371 |
STRINGi | 7227.FBpp0089115 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 442 – 480 | WD 1Add BLAST | 39 | |
Repeati | 488 – 527 | WD 2Add BLAST | 40 | |
Repeati | 532 – 571 | WD 3Add BLAST | 40 | |
Repeati | 574 – 613 | WD 4Add BLAST | 40 | |
Repeati | 615 – 654 | WD 5Add BLAST | 40 | |
Repeati | 656 – 695 | WD 6Add BLAST | 40 | |
Repeati | 697 – 730 | WD 7Add BLAST | 34 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 144 – 411 | DisorderedSequence analysisAdd BLAST | 268 | |
Regioni | 206 – 267 | CCN domainAdd BLAST | 62 | |
Regioni | 262 – 425 | Binding to basic helix-loop-helix domainAdd BLAST | 164 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 227 – 230 | Nuclear localization signalSequence analysis | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 204 – 250 | Basic and acidic residuesSequence analysisAdd BLAST | 47 | |
Compositional biasi | 258 – 280 | Basic and acidic residuesSequence analysisAdd BLAST | 23 | |
Compositional biasi | 281 – 308 | Polar residuesSequence analysisAdd BLAST | 28 | |
Compositional biasi | 341 – 384 | Pro residuesSequence analysisAdd BLAST | 44 |
Sequence similaritiesi
Keywords - Domaini
Repeat, WD repeatPhylogenomic databases
eggNOGi | KOG0639, Eukaryota |
GeneTreei | ENSGT01030000234519 |
InParanoidi | P16371 |
PhylomeDBi | P16371 |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
InterProi | View protein in InterPro IPR005617, Groucho/TLE_N IPR009146, Groucho_enhance IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR019775, WD40_repeat_CS IPR036322, WD40_repeat_dom_sf |
PANTHERi | PTHR10814, PTHR10814, 2 hits |
Pfami | View protein in Pfam PF03920, TLE_N, 1 hit PF00400, WD40, 3 hits |
PRINTSi | PR01850, GROUCHOFAMLY |
SMARTi | View protein in SMART SM00320, WD40, 7 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS00678, WD_REPEATS_1, 2 hits PS50082, WD_REPEATS_2, 3 hits PS50294, WD_REPEATS_REGION, 2 hits |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MYPSPVRHPA AGGPPPQGPI KFTIADTLER IKEEFNFLQA QYHSIKLECE
60 70 80 90 100
KLSNEKTEMQ RHYVMYYEMS YGLNVEMHKQ TEIAKRLNTL INQLLPFLQA
110 120 130 140 150
DHQQQVLQAV ERAKQVTMQE LNLIIGHQQQ HGIQQLLQQI HAQQVPGGPP
160 170 180 190 200
QPMGALNPFG ALGATMGLPH GPQGLLNKPP EHHRPDIKPT GLEGPAAAEE
210 220 230 240 250
RLRNSVSPAD REKYRTRSPL DIENDSKRRK DEKLQEDEGE KSDQDLVVDV
260 270 280 290 300
ANEMESHSPR PNGEHVSMEV RDRESLNGER LEKPSSSGIK QERPPSRSGS
310 320 330 340 350
SSSRSTPSLK TKDMEKPGTP GAKARTPTPN AAAPAPGVNP KQMMPQGPPP
360 370 380 390 400
AGYPGAPYQR PADPYQRPPS DPAYGRPPPM PYDPHAHVRT NGIPHPSALT
410 420 430 440 450
GGKPAYSFHM NGEGSLQPVP FPPDALVGVG IPRHARQINT LSHGEVVCAV
460 470 480 490 500
TISNPTKYVY TGGKGCVKVW DISQPGNKNP VSQLDCLQRD NYIRSVKLLP
510 520 530 540 550
DGRTLIVGGE ASNLSIWDLA SPTPRIKAEL TSAAPACYAL AISPDSKVCF
560 570 580 590 600
SCCSDGNIAV WDLHNEILVR QFQGHTDGAS CIDISPDGSR LWTGGLDNTV
610 620 630 640 650
RSWDLREGRQ LQQHDFSSQI FSLGYCPTGD WLAVGMENSH VEVLHASKPD
660 670 680 690 700
KYQLHLHESC VLSLRFAACG KWFVSTGKDN LLNAWRTPYG ASIFQSKETS
710 720 730
SVLSCDISTD DKYIVTGSGD KKATVYEVIY
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0B4LHP1 | A0A0B4LHP1_DROME | Groucho, isoform J | gro 10, anon-WO0118547.385, BcDNA.LD33829, BcDNA:LD33829, BEST:GM01575 | 717 | Annotation score: | ||
A0A0B4JDF1 | A0A0B4JDF1_DROME | Groucho, isoform G | gro 10, anon-WO0118547.385, BcDNA.LD33829, BcDNA:LD33829, BEST:GM01575 | 728 | Annotation score: | ||
A0A0B4K6L6 | A0A0B4K6L6_DROME | Groucho, isoform H | gro 10, anon-WO0118547.385, BcDNA.LD33829, BcDNA:LD33829, BEST:GM01575 | 709 | Annotation score: | ||
A0A0B4KH09 | A0A0B4KH09_DROME | Groucho, isoform I | gro 10, anon-WO0118547.385, BcDNA.LD33829, BcDNA:LD33829, BEST:GM01575 | 698 | Annotation score: | ||
A0A0B4JD02 | A0A0B4JD02_DROME | Groucho, isoform F | gro 10, anon-WO0118547.385, BcDNA.LD33829, BcDNA:LD33829, BEST:GM01575 | 719 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 41 | Q → H in AAA28512 (PubMed:3142687).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_022308 | 127 – 137 | Missing in isoform A. 2 PublicationsAdd BLAST | 11 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M20571 mRNA Translation: AAA28512.1 AE014297 Genomic DNA Translation: AAF56557.1 AE014297 Genomic DNA Translation: AAN14068.1 AE014297 Genomic DNA Translation: AAN14069.1 AE014297 Genomic DNA Translation: AAS65218.1 AF145695 mRNA Translation: AAD38670.1 |
PIRi | A30047 |
RefSeqi | NP_001189293.1, NM_001202364.1 [P16371-2] NP_524514.2, NM_079790.5 [P16371-2] NP_733133.1, NM_170254.3 [P16371-2] NP_733134.1, NM_170255.4 [P16371-2] NP_733135.1, NM_170256.2 [P16371-2] NP_996298.1, NM_206575.3 [P16371-1] |
Genome annotation databases
EnsemblMetazoai | FBtr0084962; FBpp0084336; FBgn0001139 [P16371-2] FBtr0084963; FBpp0084337; FBgn0001139 [P16371-2] FBtr0084964; FBpp0084338; FBgn0001139 [P16371-2] FBtr0084965; FBpp0084339; FBgn0001139 [P16371-2] FBtr0084966; FBpp0089115; FBgn0001139 [P16371-1] FBtr0302951; FBpp0292077; FBgn0001139 [P16371-2] |
GeneIDi | 43162 |
KEGGi | dme:Dmel_CG8384 |
UCSCi | CG8384-RA, d. melanogaster |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M20571 mRNA Translation: AAA28512.1 AE014297 Genomic DNA Translation: AAF56557.1 AE014297 Genomic DNA Translation: AAN14068.1 AE014297 Genomic DNA Translation: AAN14069.1 AE014297 Genomic DNA Translation: AAS65218.1 AF145695 mRNA Translation: AAD38670.1 |
PIRi | A30047 |
RefSeqi | NP_001189293.1, NM_001202364.1 [P16371-2] NP_524514.2, NM_079790.5 [P16371-2] NP_733133.1, NM_170254.3 [P16371-2] NP_733134.1, NM_170255.4 [P16371-2] NP_733135.1, NM_170256.2 [P16371-2] NP_996298.1, NM_206575.3 [P16371-1] |
3D structure databases
SMRi | P16371 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 68062, 87 interactors |
DIPi | DIP-40N |
ELMi | P16371 |
IntActi | P16371, 39 interactors |
MINTi | P16371 |
STRINGi | 7227.FBpp0089115 |
PTM databases
iPTMneti | P16371 |
Proteomic databases
PaxDbi | P16371 |
Genome annotation databases
EnsemblMetazoai | FBtr0084962; FBpp0084336; FBgn0001139 [P16371-2] FBtr0084963; FBpp0084337; FBgn0001139 [P16371-2] FBtr0084964; FBpp0084338; FBgn0001139 [P16371-2] FBtr0084965; FBpp0084339; FBgn0001139 [P16371-2] FBtr0084966; FBpp0089115; FBgn0001139 [P16371-1] FBtr0302951; FBpp0292077; FBgn0001139 [P16371-2] |
GeneIDi | 43162 |
KEGGi | dme:Dmel_CG8384 |
UCSCi | CG8384-RA, d. melanogaster |
Organism-specific databases
CTDi | 43162 |
FlyBasei | FBgn0001139, gro |
VEuPathDBi | VectorBase:FBgn0001139 |
Phylogenomic databases
eggNOGi | KOG0639, Eukaryota |
GeneTreei | ENSGT01030000234519 |
InParanoidi | P16371 |
PhylomeDBi | P16371 |
Enzyme and pathway databases
Reactomei | R-DME-201722, Formation of the beta-catenin:TCF transactivating complex R-DME-209421, Transcription activation by ARM R-DME-209441, WG ligand not bound to FZ receptors R-DME-3769402, Deactivation of the beta-catenin transactivating complex R-DME-4641265, Repression of WNT target genes |
SignaLinki | P16371 |
Miscellaneous databases
BioGRID-ORCSi | 43162, 1 hit in 3 CRISPR screens |
ChiTaRSi | gro, fly |
GenomeRNAii | 43162 |
PROi | PR:P16371 |
Gene expression databases
Bgeei | FBgn0001139, Expressed in embryo and 29 other tissues |
ExpressionAtlasi | P16371, baseline and differential |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
InterProi | View protein in InterPro IPR005617, Groucho/TLE_N IPR009146, Groucho_enhance IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR019775, WD40_repeat_CS IPR036322, WD40_repeat_dom_sf |
PANTHERi | PTHR10814, PTHR10814, 2 hits |
Pfami | View protein in Pfam PF03920, TLE_N, 1 hit PF00400, WD40, 3 hits |
PRINTSi | PR01850, GROUCHOFAMLY |
SMARTi | View protein in SMART SM00320, WD40, 7 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS00678, WD_REPEATS_1, 2 hits PS50082, WD_REPEATS_2, 3 hits PS50294, WD_REPEATS_REGION, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | GROU_DROME | |
Accessioni | P16371Primary (citable) accession number: P16371 Secondary accession number(s): A4V3F6 Q9V3F7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1990 |
Last sequence update: | January 9, 2007 | |
Last modified: | February 23, 2022 | |
This is version 213 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families