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Protein

Peptidase 1

Gene

DERF1

Organism
Dermatophagoides farinae (American house dust mite)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Thiol protease that hydrolyzes proteins, with a preference for Phe or basic residues.

Catalytic activityi

Broad endopeptidase specificity.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei133By similarity1
Active sitei269By similarity1
Active sitei288By similarity1

GO - Molecular functioni

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease

Protein family/group databases

MEROPSiC01.073

Names & Taxonomyi

Protein namesi
Recommended name:
Peptidase 1 (EC:3.4.22.65)
Alternative name(s):
Allergen Der f I
Major mite fecal allergen Der f 1
Allergen: Der f 1
Gene namesi
Name:DERF1
OrganismiDermatophagoides farinae (American house dust mite)
Taxonomic identifieri6954 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaAcariAcariformesSarcoptiformesAstigmataPsoroptidiaAnalgoideaPyroglyphidaeDermatophagoidinaeDermatophagoides

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human. Common symptoms of mite allergy are bronchial asthma, allergic rhinitis and conjunctivitis.

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei295 Der f 1
5896 Der f 1.0106

Chemistry databases

ChEMBLiCHEMBL3351205

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
PropeptideiPRO_000002637419 – 98Activation peptide1 PublicationAdd BLAST80
ChainiPRO_000002637599 – 321Peptidase 1Add BLAST223

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi102 ↔ 2161 Publication
Disulfide bondi130 ↔ 1701 Publication
Glycosylationi151N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi164 ↔ 2021 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PRIDEiP16311

PTM databases

iPTMnetiP16311

Interactioni

Subunit structurei

Monomer.1 Publication

Structurei

Secondary structure

1321
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni115 – 119Combined sources5
Beta strandi129 – 131Combined sources3
Helixi133 – 150Combined sources18
Helixi158 – 164Combined sources7
Helixi175 – 185Combined sources11
Helixi190 – 192Combined sources3
Beta strandi213 – 217Combined sources5
Helixi223 – 233Combined sources11
Beta strandi237 – 244Combined sources8
Helixi246 – 250Combined sources5
Beta strandi254 – 256Combined sources3
Beta strandi263 – 265Combined sources3
Beta strandi267 – 279Combined sources13
Beta strandi282 – 288Combined sources7
Beta strandi300 – 304Combined sources5
Helixi309 – 311Combined sources3
Turni312 – 314Combined sources3
Beta strandi317 – 320Combined sources4

3D structure databases

ProteinModelPortaliP16311
SMRiP16311
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP16311

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

InterProiView protein in InterPro
IPR038765 Papain_like_cys_pep_sf
IPR025661 Pept_asp_AS
IPR000169 Pept_cys_AS
IPR025660 Pept_his_AS
IPR013128 Peptidase_C1A
IPR000668 Peptidase_C1A_C
IPR013201 Prot_inhib_I29
PANTHERiPTHR12411 PTHR12411, 1 hit
PfamiView protein in Pfam
PF00112 Peptidase_C1, 1 hit
PRINTSiPR00705 PAPAIN
SMARTiView protein in SMART
SM00848 Inhibitor_I29, 1 hit
SM00645 Pept_C1, 1 hit
SUPFAMiSSF54001 SSF54001, 1 hit
PROSITEiView protein in PROSITE
PS00640 THIOL_PROTEASE_ASN, 1 hit
PS00139 THIOL_PROTEASE_CYS, 1 hit
PS00639 THIOL_PROTEASE_HIS, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P16311-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFVLAIASL LVLSTVYARP ASIKTFEEFK KAFNKNYATV EEEEVARKNF
60 70 80 90 100
LESLKYVEAN KGAINHLSDL SLDEFKNRYL MSAEAFEQLK TQFDLNAETS
110 120 130 140 150
ACRINSVNVP SELDLRSLRT VTPIRMQGGC GSCWAFSGVA ATESAYLAYR
160 170 180 190 200
NTSLDLSEQE LVDCASQHGC HGDTIPRGIE YIQQNGVVEE RSYPYVAREQ
210 220 230 240 250
RCRRPNSQHY GISNYCQIYP PDVKQIREAL TQTHTAIAVI IGIKDLRAFQ
260 270 280 290 300
HYDGRTIIQH DNGYQPNYHA VNIVGYGSTQ GDDYWIVRNS WDTTWGDSGY
310 320
GYFQAGNNLM MIEQYPYVVI M
Length:321
Mass (Da):36,435
Last modified:February 1, 1995 - v2
Checksum:i04523E54EEBB476E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti201R → Q in CAA46316 (Ref. 2) Curated1
Sequence conflicti282D → V in CAA46316 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65196 Genomic DNA Translation: CAA46316.1
PIRiA27634
A61500

Similar proteinsi

Entry informationi

Entry nameiPEPT1_DERFA
AccessioniPrimary (citable) accession number: P16311
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: February 1, 1995
Last modified: June 20, 2018
This is version 104 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

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