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UniProtKB - P16294 (FA9_MOUSE)
Protein
Coagulation factor IX
Gene
F9
Organism
Mus musculus (Mouse)
Status
Functioni
Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca2+ ions, phospholipids, and factor VIIIa.
By similarityCatalytic activityi
- Selective cleavage of Arg-|-Ile bond in factor X to form factor Xa.By similarity EC:3.4.21.22
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 47 | Calcium 1; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 48 | Calcium 2By similarity | 1 | |
Metal bindingi | 53 | Calcium 1; via 4-carboxyglutamateBy similarity | 1 | |
Metal bindingi | 53 | Calcium 2; via 4-carboxyglutamateBy similarity | 1 | |
Metal bindingi | 54 | Calcium 2; via 4-carboxyglutamateBy similarity | 1 | |
Metal bindingi | 54 | Calcium 3; via 4-carboxyglutamateBy similarity | 1 | |
Metal bindingi | 61 | Calcium 4; via 4-carboxyglutamateBy similarity | 1 | |
Metal bindingi | 61 | Magnesium 1; via 4-carboxyglutamateBy similarity | 1 | |
Metal bindingi | 63 | Calcium 1; via 4-carboxyglutamateBy similarity | 1 | |
Metal bindingi | 63 | Calcium 2; via 4-carboxyglutamateBy similarity | 1 | |
Metal bindingi | 63 | Calcium 3; via 4-carboxyglutamateBy similarity | 1 | |
Metal bindingi | 66 | Calcium 4; via 4-carboxyglutamateBy similarity | 1 | |
Metal bindingi | 66 | Magnesium 1; via 4-carboxyglutamateBy similarity | 1 | |
Metal bindingi | 67 | Calcium 1; via 4-carboxyglutamateBy similarity | 1 | |
Metal bindingi | 72 | Calcium 5; via 4-carboxyglutamateBy similarity | 1 | |
Metal bindingi | 72 | Magnesium 2; via 4-carboxyglutamateBy similarity | 1 | |
Metal bindingi | 73 | Calcium 2; via 4-carboxyglutamateBy similarity | 1 | |
Metal bindingi | 73 | Calcium 3; via 4-carboxyglutamateBy similarity | 1 | |
Metal bindingi | 76 | Calcium 3; via 4-carboxyglutamateBy similarity | 1 | |
Metal bindingi | 76 | Calcium 5; via 4-carboxyglutamateBy similarity | 1 | |
Metal bindingi | 76 | Magnesium 2; via 4-carboxyglutamateBy similarity | 1 | |
Metal bindingi | 82 | Calcium 6; via 4-carboxyglutamateBy similarity | 1 | |
Metal bindingi | 82 | Magnesium 3; via 4-carboxyglutamateBy similarity | 1 | |
Metal bindingi | 86 | Calcium 6; via 4-carboxyglutamateBy similarity | 1 | |
Metal bindingi | 86 | Magnesium 3; via 4-carboxyglutamateBy similarity | 1 | |
Metal bindingi | 93 | Calcium 7By similarity | 1 | |
Metal bindingi | 94 | Calcium 7; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 96 | Calcium 7By similarity | 1 | |
Metal bindingi | 110 | Calcium 7By similarity | 1 | |
Metal bindingi | 111 | Calcium 7; via carbonyl oxygenBy similarity | 1 | |
Active sitei | 277 | Charge relay systemBy similarity | 1 | |
Metal bindingi | 291 | Calcium 8By similarity | 1 | |
Metal bindingi | 293 | Calcium 8; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 298 | Calcium 8By similarity | 1 | |
Metal bindingi | 301 | Calcium 8By similarity | 1 | |
Active sitei | 325 | Charge relay systemBy similarity | 1 | |
Active sitei | 421 | Charge relay systemBy similarity | 1 |
GO - Molecular functioni
- calcium ion binding Source: UniProtKB
- endopeptidase activity Source: UniProtKB
- peptidase activity Source: MGI
- serine-type endopeptidase activity Source: UniProtKB-EC
GO - Biological processi
- blood coagulation Source: MGI
- blood coagulation, intrinsic pathway Source: MGI
- proteolysis Source: MGI
- zymogen activation Source: UniProtKB
Keywordsi
Molecular function | Hydrolase, Protease, Serine protease |
Biological process | Blood coagulation, Hemostasis |
Ligand | Calcium, Magnesium, Metal-binding |
Enzyme and pathway databases
Reactomei | R-MMU-140834, Extrinsic Pathway of Fibrin Clot Formation R-MMU-140837, Intrinsic Pathway of Fibrin Clot Formation R-MMU-159740, Gamma-carboxylation of protein precursors R-MMU-159763, Transport of gamma-carboxylated protein precursors from the endoplasmic reticulum to the Golgi apparatus R-MMU-159782, Removal of aminoterminal propeptides from gamma-carboxylated proteins |
Protein family/group databases
MEROPSi | S01.214 |
Names & Taxonomyi
Protein namesi | Recommended name: Coagulation factor IX (EC:3.4.21.22By similarity)Alternative name(s): Christmas factor Cleaved into the following 2 chains: |
Gene namesi | Name:F9 Synonyms:Cf9 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:88384, F9 |
VEuPathDBi | HostDB:ENSMUSG00000031138 |
Subcellular locationi
Extracellular region or secreted
- Secreted By similarity
Extracellular region or secreted
- extracellular space Source: UniProtKB
Other locations
- membrane Source: MGI
- protein-containing complex Source: MGI
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 28 | Sequence analysisAdd BLAST | 28 | |
PropeptideiPRO_0000027760 | 29 – 46 | By similarityAdd BLAST | 18 | |
ChainiPRO_0000027761 | 47 – 471 | Coagulation factor IXAdd BLAST | 425 | |
ChainiPRO_0000027762 | 47 – 192 | Coagulation factor IXa light chainAdd BLAST | 146 | |
PropeptideiPRO_0000027763 | 193 – 236 | Activation peptideBy similarityAdd BLAST | 44 | |
ChainiPRO_0000027764 | 237 – 471 | Coagulation factor IXa heavy chainAdd BLAST | 235 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 53 | 4-carboxyglutamatePROSITE-ProRule annotationBy similarity | 1 | |
Modified residuei | 54 | 4-carboxyglutamatePROSITE-ProRule annotationBy similarity | 1 | |
Modified residuei | 61 | 4-carboxyglutamatePROSITE-ProRule annotationBy similarity | 1 | |
Modified residuei | 63 | 4-carboxyglutamatePROSITE-ProRule annotationBy similarity | 1 | |
Disulfide bondi | 64 ↔ 69 | By similarity | ||
Modified residuei | 66 | 4-carboxyglutamatePROSITE-ProRule annotationBy similarity | 1 | |
Modified residuei | 67 | 4-carboxyglutamatePROSITE-ProRule annotationBy similarity | 1 | |
Modified residuei | 72 | 4-carboxyglutamatePROSITE-ProRule annotationBy similarity | 1 | |
Modified residuei | 73 | 4-carboxyglutamatePROSITE-ProRule annotationBy similarity | 1 | |
Modified residuei | 76 | 4-carboxyglutamatePROSITE-ProRule annotationBy similarity | 1 | |
Modified residuei | 79 | 4-carboxyglutamatePROSITE-ProRule annotationBy similarity | 1 | |
Modified residuei | 82 | 4-carboxyglutamatePROSITE-ProRule annotationBy similarity | 1 | |
Glycosylationi | 85 | O-linked (GalNAc...) threonineBy similarity | 1 | |
Modified residuei | 86 | 4-carboxyglutamatePROSITE-ProRule annotationBy similarity | 1 | |
Disulfide bondi | 97 ↔ 108 | By similarity | ||
Glycosylationi | 99 | O-linked (Glc...) serineBy similarity | 1 | |
Disulfide bondi | 102 ↔ 117 | By similarity | ||
Modified residuei | 110 | (3R)-3-hydroxyaspartateBy similarity | 1 | |
Modified residuei | 114 | PhosphoserineBy similarity | 1 | |
Disulfide bondi | 119 ↔ 128 | By similarity | ||
Disulfide bondi | 134 ↔ 145 | By similarity | ||
Disulfide bondi | 141 ↔ 155 | By similarity | ||
Disulfide bondi | 157 ↔ 170 | By similarity | ||
Disulfide bondi | 178 ↔ 345 | Interchain (between light and heavy chains)By similarity | ||
Modified residuei | 202 | SulfotyrosineBy similarity | 1 | |
Glycosylationi | 204 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 205 | PhosphoserineBy similarity | 1 | |
Modified residuei | 206 | Phosphothreonine; alternateBy similarity | 1 | |
Glycosylationi | 206 | O-linked (GalNAc...) threonine; alternateBy similarity | 1 | |
Glycosylationi | 223 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 225 | O-linked (GalNAc...) threonineBy similarity | 1 | |
Glycosylationi | 235 | O-linked (GalNAc...) threonineBy similarity | 1 | |
Disulfide bondi | 262 ↔ 278 | By similarity | ||
Disulfide bondi | 392 ↔ 406 | By similarity | ||
Disulfide bondi | 417 ↔ 445 | By similarity |
Post-translational modificationi
Activated by factor XIa, which excises the activation peptide. The propeptide can also be removed by snake venom protease.By similarity
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.By similarity
Predominantly O-glucosylated at Ser-99 by POGLUT1 in vitro.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 192 – 193 | Cleavage; by factor XIaBy similarity | 2 | |
Sitei | 236 – 237 | Cleavage; by factor XIaBy similarity | 2 |
Keywords - PTMi
Cleavage on pair of basic residues, Disulfide bond, Gamma-carboxyglutamic acid, Glycoprotein, Hydroxylation, Phosphoprotein, Sulfation, ZymogenProteomic databases
CPTACi | non-CPTAC-3415 non-CPTAC-3576 non-CPTAC-5605 |
MaxQBi | P16294 |
PaxDbi | P16294 |
PRIDEi | P16294 |
ProteomicsDBi | 277033 |
TopDownProteomicsi | P16294 |
PTM databases
GlyGeni | P16294, 7 sites |
iPTMneti | P16294 |
PhosphoSitePlusi | P16294 |
Expressioni
Tissue specificityi
Detected in liver.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000031138, Expressed in liver and 43 other tissues |
Genevisiblei | P16294, MM |
Interactioni
Subunit structurei
Heterodimer of a light chain and a heavy chain; disulfide-linked.
Interacts with SERPINC1.
By similarityProtein-protein interaction databases
ComplexPortali | CPX-5101, Coagulation factor IXa complex |
STRINGi | 10090.ENSMUSP00000033477 |
Miscellaneous databases
RNActi | P16294, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 47 – 92 | GlaPROSITE-ProRule annotationAdd BLAST | 46 | |
Domaini | 93 – 129 | EGF-like 1; calcium-bindingPROSITE-ProRule annotationAdd BLAST | 37 | |
Domaini | 130 – 171 | EGF-like 2PROSITE-ProRule annotationAdd BLAST | 42 | |
Domaini | 237 – 469 | Peptidase S1PROSITE-ProRule annotationAdd BLAST | 233 |
Domaini
Calcium binds to the gamma-carboxyglutamic acid (Gla) residues in the Gla domain. Calcium can also bind, with stronger affinity, to another site beyond the Gla domain. Under physiological ion concentrations, Ca2+ is displaced by Mg2+ from some of the gammaglutamate residues in the N-terminal Gla domain. This leads to a subtle conformation change that may affect the interaction with its binding protein.By similarity
Sequence similaritiesi
Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Keywords - Domaini
EGF-like domain, Repeat, SignalPhylogenomic databases
eggNOGi | ENOG502QUEV, Eukaryota |
GeneTreei | ENSGT00940000159516 |
HOGENOMi | CLU_006842_19_5_1 |
InParanoidi | P16294 |
OMAi | SCTEGYQ |
OrthoDBi | 1314811at2759 |
PhylomeDBi | P16294 |
TreeFami | TF327329 |
Family and domain databases
CDDi | cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits 4.10.740.10, 1 hit |
InterProi | View protein in InterPro IPR017857, Coagulation_fac-like_Gla_dom IPR035694, Coagulation_factor_IX IPR001881, EGF-like_Ca-bd_dom IPR000742, EGF-like_dom IPR000152, EGF-type_Asp/Asn_hydroxyl_site IPR018097, EGF_Ca-bd_CS IPR035972, GLA-like_dom_SF IPR000294, GLA_domain IPR012224, Pept_S1A_FX IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
PANTHERi | PTHR24278:SF31, PTHR24278:SF31, 1 hit |
Pfami | View protein in Pfam PF00008, EGF, 1 hit PF00594, Gla, 1 hit PF00089, Trypsin, 1 hit |
PIRSFi | PIRSF001143, Factor_X, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN PR00001, GLABLOOD |
SMARTi | View protein in SMART SM00181, EGF, 2 hits SM00179, EGF_CA, 1 hit SM00069, GLA, 1 hit SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit SSF57630, SSF57630, 1 hit |
PROSITEi | View protein in PROSITE PS00010, ASX_HYDROXYL, 1 hit PS00022, EGF_1, 1 hit PS01186, EGF_2, 2 hits PS50026, EGF_3, 1 hit PS01187, EGF_CA, 1 hit PS00011, GLA_1, 1 hit PS50998, GLA_2, 1 hit PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P16294-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKHLNTVMAE SPALITIFLL GYLLSTECAV FLDRENATKI LTRPKRYNSG
60 70 80 90 100
KLEEFVRGNL ERECIEERCS FEEAREVFEN TEKTTEFWKQ YVDGDQCESN
110 120 130 140 150
PCLNGGICKD DISSYECWCQ VGFEGRNCEL DATCNIKNGR CKQFCKNSPD
160 170 180 190 200
NKVICSCTEG YQLAEDQKSC EPTVPFPCGR ASISYSSKKI TRAETVFSNM
210 220 230 240 250
DYENSTEAVF IQDDITDGAI LNNVTESSES LNDFTRVVGG ENAKPGQIPW
260 270 280 290 300
QVILNGEIEA FCGGAIINEK WIVTAAHCLK PGDKIEVVAG EYNIDKKEDT
310 320 330 340 350
EQRRNVIRTI PHHQYNATIN KYSHDIALLE LDKPLILNSY VTPICVANRE
360 370 380 390 400
YTNIFLKFGS GYVSGWGKVF NKGRQASILQ YLRVPLVDRA TCLRSTTFTI
410 420 430 440 450
YNNMFCAGYR EGGKDSCEGD SGGPHVTEVE GTSFLTGIIS WGEECAMKGK
460 470
YGIYTKVSRY VNWIKEKTKL T
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 375 | Q → H in AAA37629 (PubMed:2323576).Curated | 1 | |
Sequence conflicti | 400 | I → T in AAA37629 (PubMed:2323576).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK149372 mRNA Translation: BAE28840.1 M23109 mRNA Translation: AAA37629.1 M26236 mRNA Translation: AAA37630.1 |
CCDSi | CCDS30158.1 |
PIRi | JQ0419 |
RefSeqi | NP_001292726.1, NM_001305797.1 NP_032005.1, NM_007979.2 |
Genome annotation databases
Ensembli | ENSMUST00000033477; ENSMUSP00000033477; ENSMUSG00000031138 |
GeneIDi | 14071 |
KEGGi | mmu:14071 |
UCSCi | uc009thw.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK149372 mRNA Translation: BAE28840.1 M23109 mRNA Translation: AAA37629.1 M26236 mRNA Translation: AAA37630.1 |
CCDSi | CCDS30158.1 |
PIRi | JQ0419 |
RefSeqi | NP_001292726.1, NM_001305797.1 NP_032005.1, NM_007979.2 |
3D structure databases
AlphaFoldDBi | P16294 |
SMRi | P16294 |
ModBasei | Search... |
Protein-protein interaction databases
ComplexPortali | CPX-5101, Coagulation factor IXa complex |
STRINGi | 10090.ENSMUSP00000033477 |
Protein family/group databases
MEROPSi | S01.214 |
PTM databases
GlyGeni | P16294, 7 sites |
iPTMneti | P16294 |
PhosphoSitePlusi | P16294 |
Proteomic databases
CPTACi | non-CPTAC-3415 non-CPTAC-3576 non-CPTAC-5605 |
MaxQBi | P16294 |
PaxDbi | P16294 |
PRIDEi | P16294 |
ProteomicsDBi | 277033 |
TopDownProteomicsi | P16294 |
Protocols and materials databases
Antibodypediai | 367, 909 antibodies from 39 providers |
DNASUi | 14071 |
Genome annotation databases
Ensembli | ENSMUST00000033477; ENSMUSP00000033477; ENSMUSG00000031138 |
GeneIDi | 14071 |
KEGGi | mmu:14071 |
UCSCi | uc009thw.2, mouse |
Organism-specific databases
CTDi | 2158 |
MGIi | MGI:88384, F9 |
VEuPathDBi | HostDB:ENSMUSG00000031138 |
Phylogenomic databases
eggNOGi | ENOG502QUEV, Eukaryota |
GeneTreei | ENSGT00940000159516 |
HOGENOMi | CLU_006842_19_5_1 |
InParanoidi | P16294 |
OMAi | SCTEGYQ |
OrthoDBi | 1314811at2759 |
PhylomeDBi | P16294 |
TreeFami | TF327329 |
Enzyme and pathway databases
Reactomei | R-MMU-140834, Extrinsic Pathway of Fibrin Clot Formation R-MMU-140837, Intrinsic Pathway of Fibrin Clot Formation R-MMU-159740, Gamma-carboxylation of protein precursors R-MMU-159763, Transport of gamma-carboxylated protein precursors from the endoplasmic reticulum to the Golgi apparatus R-MMU-159782, Removal of aminoterminal propeptides from gamma-carboxylated proteins |
Miscellaneous databases
BioGRID-ORCSi | 14071, 1 hit in 59 CRISPR screens |
ChiTaRSi | F9, mouse |
PROi | PR:P16294 |
RNActi | P16294, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000031138, Expressed in liver and 43 other tissues |
Genevisiblei | P16294, MM |
Family and domain databases
CDDi | cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits 4.10.740.10, 1 hit |
InterProi | View protein in InterPro IPR017857, Coagulation_fac-like_Gla_dom IPR035694, Coagulation_factor_IX IPR001881, EGF-like_Ca-bd_dom IPR000742, EGF-like_dom IPR000152, EGF-type_Asp/Asn_hydroxyl_site IPR018097, EGF_Ca-bd_CS IPR035972, GLA-like_dom_SF IPR000294, GLA_domain IPR012224, Pept_S1A_FX IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
PANTHERi | PTHR24278:SF31, PTHR24278:SF31, 1 hit |
Pfami | View protein in Pfam PF00008, EGF, 1 hit PF00594, Gla, 1 hit PF00089, Trypsin, 1 hit |
PIRSFi | PIRSF001143, Factor_X, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN PR00001, GLABLOOD |
SMARTi | View protein in SMART SM00181, EGF, 2 hits SM00179, EGF_CA, 1 hit SM00069, GLA, 1 hit SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit SSF57630, SSF57630, 1 hit |
PROSITEi | View protein in PROSITE PS00010, ASX_HYDROXYL, 1 hit PS00022, EGF_1, 1 hit PS01186, EGF_2, 2 hits PS50026, EGF_3, 1 hit PS01187, EGF_CA, 1 hit PS00011, GLA_1, 1 hit PS50998, GLA_2, 1 hit PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | FA9_MOUSE | |
Accessioni | P16294Primary (citable) accession number: P16294 Secondary accession number(s): Q3UES1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1990 |
Last sequence update: | January 9, 2007 | |
Last modified: | May 25, 2022 | |
This is version 214 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families