UniProtKB - P16259 (CAN3_RAT)
Calpain-3
Capn3
Functioni
Calcium-regulated non-lysosomal thiol-protease. Proteolytically cleaves CTBP1 at 'His-399'. Mediates, with UTP25, the proteasome-independent degradation of p53/TP53.
By similarityCatalytic activityi
- Broad endopeptidase activity. EC:3.4.22.54
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 129 | PROSITE-ProRule annotation | 1 | |
Active sitei | 334 | PROSITE-ProRule annotation | 1 | |
Active sitei | 358 | PROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Calcium bindingi | 662 – 672 | 1By similarityAdd BLAST | 11 | |
Calcium bindingi | 705 – 716 | 2PROSITE-ProRule annotationBy similarityAdd BLAST | 12 | |
Calcium bindingi | 735 – 741 | 3PROSITE-ProRule annotationBy similarity | 7 | |
Calcium bindingi | 800 – 806 | 4By similarity | 7 |
GO - Molecular functioni
- calcium-dependent cysteine-type endopeptidase activity Source: RGD
- calcium ion binding Source: UniProtKB
- catalytic activity Source: UniProtKB
- enzyme binding Source: UniProtKB
- ligase regulator activity Source: UniProtKB
- molecular adaptor activity Source: UniProtKB
- peptidase activity Source: RGD
- signaling receptor binding Source: UniProtKB
- sodium ion binding Source: UniProtKB
- structural constituent of muscle Source: UniProtKB
- titin binding Source: UniProtKB
GO - Biological processi
- calcium-dependent self proteolysis Source: UniProtKB
- cellular response to calcium ion Source: UniProtKB
- cellular response to salt stress Source: UniProtKB
- G1 to G0 transition involved in cell differentiation Source: RGD
- muscle structure development Source: UniProtKB
- myofibril assembly Source: UniProtKB
- negative regulation of apoptotic process Source: UniProtKB
- negative regulation of protein sumoylation Source: UniProtKB
- negative regulation of transcription, DNA-templated Source: UniProtKB
- positive regulation of NF-kappaB transcription factor activity Source: UniProtKB
- positive regulation of proteolysis Source: UniProtKB
- positive regulation of release of sequestered calcium ion into cytosol Source: UniProtKB
- positive regulation of satellite cell activation involved in skeletal muscle regeneration Source: UniProtKB
- positive regulation of transcription, DNA-templated Source: UniProtKB
- programmed cell death Source: RGD
- protein catabolic process Source: UniProtKB
- protein-containing complex assembly Source: UniProtKB
- protein destabilization Source: RGD
- protein localization to membrane Source: UniProtKB
- proteolysis Source: UniProtKB
- regulation of catalytic activity Source: UniProtKB
- regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
- regulation of myoblast differentiation Source: RGD
- response to calcium ion Source: RGD
- response to muscle activity Source: UniProtKB
- response to xenobiotic stimulus Source: RGD
- sarcomere organization Source: UniProtKB
- self proteolysis Source: UniProtKB
- sodium-dependent self proteolysis Source: RGD
Keywordsi
Molecular function | Hydrolase, Protease, Thiol protease |
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
BRENDAi | 3.4.22.54, 5301 |
Reactomei | R-RNO-1474228, Degradation of the extracellular matrix |
Protein family/group databases
MEROPSi | C02.004 |
Names & Taxonomyi
Protein namesi | Recommended name: Calpain-3 (EC:3.4.22.54)Alternative name(s): Calcium-activated neutral proteinase 3 Short name: CANP 3 Calpain L3 Calpain p94 Muscle-specific calcium-activated neutral protease 3 |
Gene namesi | Name:Capn3 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2269, Capn3 |
Subcellular locationi
Cytosol
- cytosol Source: UniProtKB
Nucleus
Plasma Membrane
- plasma membrane Source: UniProtKB
- T-tubule Source: UniProtKB
Other locations
- cytoplasm Source: RGD
- myofibril Source: UniProtKB
- protein-containing complex Source: UniProtKB
- Z disc Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000207710 | 1 – 821 | Calpain-3Add BLAST | 821 |
Proteomic databases
PaxDbi | P16259 |
PTM databases
PhosphoSitePlusi | P16259 |
Expressioni
Tissue specificityi
Interactioni
Subunit structurei
Homodimer; via EF-hand domain 4.
Interacts with TTN/titin.
Interacts with CMYA5; this interaction, which results in CMYA5 proteolysis, may protect CAPN3 from autolysis.
Interacts with SIMC1.
Interacts with UTP25; the interaction is required for CAPN3 translocation to the nucleolus.
By similarityGO - Molecular functioni
- enzyme binding Source: UniProtKB
- signaling receptor binding Source: UniProtKB
- titin binding Source: UniProtKB
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000011761 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 74 – 417 | Calpain catalyticPROSITE-ProRule annotationAdd BLAST | 344 | |
Domaini | 649 – 683 | EF-hand 1PROSITE-ProRule annotationAdd BLAST | 35 | |
Domaini | 692 – 725 | EF-hand 2PROSITE-ProRule annotationAdd BLAST | 34 | |
Domaini | 722 – 757 | EF-hand 3PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 787 – 821 | EF-hand 4PROSITE-ProRule annotationAdd BLAST | 35 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 37 | DisorderedSequence analysisAdd BLAST | 37 | |
Regioni | 418 – 586 | Domain IIIAdd BLAST | 169 | |
Regioni | 587 – 649 | LinkerAdd BLAST | 63 | |
Regioni | 603 – 651 | DisorderedSequence analysisAdd BLAST | 49 | |
Regioni | 650 – 820 | Domain IVAdd BLAST | 171 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 613 – 633 | Basic and acidic residuesSequence analysisAdd BLAST | 21 |
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0045, Eukaryota |
InParanoidi | P16259 |
OrthoDBi | 704215at2759 |
PhylomeDBi | P16259 |
Family and domain databases
CDDi | cd00214, Calpain_III, 1 hit cd00044, CysPc, 1 hit |
InterProi | View protein in InterPro IPR033883, C2_III IPR022684, Calpain_cysteine_protease IPR022682, Calpain_domain_III IPR022683, Calpain_III IPR036213, Calpain_III_sf IPR029531, CAPN3 IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom IPR038765, Papain-like_cys_pep_sf IPR000169, Pept_cys_AS IPR001300, Peptidase_C2_calpain_cat |
PANTHERi | PTHR10183:SF329, PTHR10183:SF329, 1 hit |
Pfami | View protein in Pfam PF01067, Calpain_III, 1 hit PF13833, EF-hand_8, 1 hit PF00648, Peptidase_C2, 1 hit |
PRINTSi | PR00704, CALPAIN |
SMARTi | View protein in SMART SM00720, calpain_III, 1 hit SM00230, CysPc, 1 hit SM00054, EFh, 3 hits |
SUPFAMi | SSF47473, SSF47473, 1 hit SSF49758, SSF49758, 1 hit SSF54001, SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS50203, CALPAIN_CAT, 1 hit PS00018, EF_HAND_1, 2 hits PS50222, EF_HAND_2, 4 hits PS00139, THIOL_PROTEASE_CYS, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MPTVISPTVA PRTGAEPRSP GPVPHPAQGK TTEAGGGHPG GIYSAIISRN
60 70 80 90 100
FPIIGVKEKT FEQLHKKCLE KKVLYLDPEF PPDETSLFYS QKFPIQFVWK
110 120 130 140 150
RPPEICENPR FIIGGANRTD ICQGDLGDCW LLAAIACLTL NERLLFRVIP
160 170 180 190 200
HDQSFTENYA GIFHFQFWRY GDWVDVVIDD CLPTYNNQLV FTKSNHRNEF
210 220 230 240 250
WSALLEKAYA KLHGSYEALK GGNTTEAMED FTGGVTEFFE IKDAPSDMYK
260 270 280 290 300
IMRKAIERGS LMGCSIDDGT NMTYGTSPSG LNMGELIARM VRNMDNSLLR
310 320 330 340 350
DSDLDPRASD DRPSRTIVPV QYETRMACGL VKGHAYSVTG LEEALFKGEK
360 370 380 390 400
VKLVRLRNPW GQVEWNGSWS DGWKDWSFVD KDEKARLQHQ VTEDGEFWMS
410 420 430 440 450
YDDFVYHFTK LEICNLTADA LESDKLQTWT VSVNEGRWVR GCSAGGCRNF
460 470 480 490 500
PDTFWTNPQY RLKLLEEDDD PDDSEVICSF LVALMQKNRR KDRKLGANLF
510 520 530 540 550
TIGFAIYEVP KEMHGNKQHL QKDFFLYNAS KARSKTYINM REVSQRFRLP
560 570 580 590 600
PSEYVIVPST YEPHQEGEFI LRVFSEKRNL SEEAENTISV DRPVKKKKNK
610 620 630 640 650
PIIFVSDRAN SNKELGVDQE AEEGKDKTGP DKQGESPQPR PGHTDQESEE
660 670 680 690 700
QQQFRNIFRQ IAGDDMEICA DELKNVLNTV VNKHKDLKTQ GFTLESCRSM
710 720 730 740 750
IALMDTDGSG RLNLQEFHHL WKKIKAWQKI FKHYDTDHSG TINSYEMRNA
760 770 780 790 800
VNDAGFHLNS QLYDIITMRY ADKHMNIDFD SFICCFVRLE GMFRAFHAFD
810 820
KDGDGIIKLN VLEWLQLTMY A
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF1LSQ2 | F1LSQ2_RAT | Calpain-3 | Capn3 | 821 | Annotation score: | ||
A0A0G2K9P9 | A0A0G2K9P9_RAT | Calpain-3 | Capn3 | 785 | Annotation score: | ||
O08702 | O08702_RAT | Calpain-3 | Capn3 | 709 | Annotation score: | ||
A0A0G2JVX0 | A0A0G2JVX0_RAT | Calpain-3 | Capn3 | 811 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J05121 mRNA Translation: AAA41790.1 |
PIRi | B34488 |
RefSeqi | NP_058813.1, NM_017117.1 |
Genome annotation databases
GeneIDi | 29155 |
KEGGi | rno:29155 |
UCSCi | RGD:2269, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J05121 mRNA Translation: AAA41790.1 |
PIRi | B34488 |
RefSeqi | NP_058813.1, NM_017117.1 |
3D structure databases
SMRi | P16259 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000011761 |
Protein family/group databases
MEROPSi | C02.004 |
PTM databases
PhosphoSitePlusi | P16259 |
Proteomic databases
PaxDbi | P16259 |
Genome annotation databases
GeneIDi | 29155 |
KEGGi | rno:29155 |
UCSCi | RGD:2269, rat |
Organism-specific databases
CTDi | 825 |
RGDi | 2269, Capn3 |
Phylogenomic databases
eggNOGi | KOG0045, Eukaryota |
InParanoidi | P16259 |
OrthoDBi | 704215at2759 |
PhylomeDBi | P16259 |
Enzyme and pathway databases
BRENDAi | 3.4.22.54, 5301 |
Reactomei | R-RNO-1474228, Degradation of the extracellular matrix |
Miscellaneous databases
PROi | PR:P16259 |
Family and domain databases
CDDi | cd00214, Calpain_III, 1 hit cd00044, CysPc, 1 hit |
InterProi | View protein in InterPro IPR033883, C2_III IPR022684, Calpain_cysteine_protease IPR022682, Calpain_domain_III IPR022683, Calpain_III IPR036213, Calpain_III_sf IPR029531, CAPN3 IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom IPR038765, Papain-like_cys_pep_sf IPR000169, Pept_cys_AS IPR001300, Peptidase_C2_calpain_cat |
PANTHERi | PTHR10183:SF329, PTHR10183:SF329, 1 hit |
Pfami | View protein in Pfam PF01067, Calpain_III, 1 hit PF13833, EF-hand_8, 1 hit PF00648, Peptidase_C2, 1 hit |
PRINTSi | PR00704, CALPAIN |
SMARTi | View protein in SMART SM00720, calpain_III, 1 hit SM00230, CysPc, 1 hit SM00054, EFh, 3 hits |
SUPFAMi | SSF47473, SSF47473, 1 hit SSF49758, SSF49758, 1 hit SSF54001, SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS50203, CALPAIN_CAT, 1 hit PS00018, EF_HAND_1, 2 hits PS50222, EF_HAND_2, 4 hits PS00139, THIOL_PROTEASE_CYS, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CAN3_RAT | |
Accessioni | P16259Primary (citable) accession number: P16259 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1990 |
Last sequence update: | August 1, 1990 | |
Last modified: | February 23, 2022 | |
This is version 174 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - SIMILARITY comments
Index of protein domains and families