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Protein

Corticosteroid 11-beta-dehydrogenase isozyme 1

Gene

Hsd11b1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes reversibly the conversion of corticosterone to 11-dehydrocorticosterone.

Catalytic activityi

An 11-beta-hydroxysteroid + NADP+ = an 11-oxosteroid + NADPH.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei166SubstrateBy similarity1
Active sitei179Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi37 – 63NADPBy similarityAdd BLAST27
Nucleotide bindingi88 – 89NADPBy similarity2
Nucleotide bindingi115 – 117NADPBy similarity3
Nucleotide bindingi179 – 183NADPBy similarity5
Nucleotide bindingi212 – 218NADPBy similarity7

GO - Molecular functioni

  • 11-beta-hydroxysteroid dehydrogenase (NADP+) activity Source: UniProtKB-EC
  • 11-beta-hydroxysteroid dehydrogenase [NAD(P)] activity Source: RGD
  • NADP binding Source: RGD
  • steroid binding Source: RGD

GO - Biological processi

  • cellular response to estradiol stimulus Source: RGD
  • glucocorticoid biosynthetic process Source: RGD
  • glucocorticoid catabolic process Source: RGD
  • regulation of pentose-phosphate shunt Source: RGD
  • response to nutrient levels Source: RGD

Keywordsi

Molecular functionOxidoreductase
Biological processLipid metabolism, Steroid metabolism
LigandNADP

Enzyme and pathway databases

BRENDAi1.1.1.146 5301
SABIO-RKiP16232

Names & Taxonomyi

Protein namesi
Recommended name:
Corticosteroid 11-beta-dehydrogenase isozyme 1 (EC:1.1.1.146)
Alternative name(s):
11-beta-hydroxysteroid dehydrogenase 1
Short name:
11-DH
Short name:
11-beta-HSD1
Gene namesi
Name:Hsd11b1
Synonyms:Hsd11
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi2834 Hsd11b1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 4CytoplasmicSequence analysis4
Transmembranei5 – 20Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST16
Topological domaini21 – 288LumenalSequence analysisAdd BLAST268

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi110D → N: Slight loss of activity. 1
Mutagenesisi179Y → F or S: Complete loss of activity. 1 Publication1
Mutagenesisi183K → R: Complete loss of activity. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL2391

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000546231 – 288Corticosteroid 11-beta-dehydrogenase isozyme 1Add BLAST288

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi158N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi203N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Glycosylated.

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiP16232

PTM databases

iPTMnetiP16232
PhosphoSitePlusiP16232

Expressioni

Tissue specificityi

Liver, kidney, testis and placenta.

Developmental stagei

Expression of both isoforms is found in 1 week-old rats. Expression increases exponentially up to 4 weeks but after this time there is no further increase up to 16 weeks.

Gene expression databases

BgeeiENSRNOG00000005861
ExpressionAtlasiP16232 baseline and differential
GenevisibleiP16232 RN

Interactioni

Subunit structurei

Homodimer.By similarity

Chemistry databases

BindingDBiP16232

Structurei

3D structure databases

ProteinModelPortaliP16232
SMRiP16232
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00910000144007
HOGENOMiHOG000010276
HOVERGENiHBG005481
InParanoidiP16232
KOiK15680
OMAiIGREMAY
PhylomeDBiP16232

Family and domain databases

InterProiView protein in InterPro
IPR036291 NAD(P)-bd_dom_sf
IPR020904 Sc_DH/Rdtase_CS
IPR002347 SDR_fam
PfamiView protein in Pfam
PF00106 adh_short, 1 hit
PRINTSiPR00081 GDHRDH
SUPFAMiSSF51735 SSF51735, 1 hit
PROSITEiView protein in PROSITE
PS00061 ADH_SHORT, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative promoter usage. AlignAdd to basket

Isoform 11-HSD1A (identifier: P16232-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKKYLLPVLV LCLGYYYSTN EEFRPEMLQG KKVIVTGASK GIGREMAYHL
60 70 80 90 100
SKMGAHVVLT ARSEEGLQKV VSRCLELGAA SAHYIAGTME DMAFAERFVV
110 120 130 140 150
EAGKLLGGLD MLILNHITQT TMSLFHDDIH SVRRSMEVNF LSYVVLSTAA
160 170 180 190 200
LPMLKQSNGS IAIISSMAGK MTQPLIASYS ASKFALDGFF STIRKEHLMT
210 220 230 240 250
KVNVSITLCV LGFIDTETAL KETSGIILSQ AAPKEECALE IIKGTVLRKD
260 270 280
EVYYDKSSWT PLLLGNPGRR IMEFLSLRSY NRDLFVSN
Length:288
Mass (Da):31,883
Last modified:October 10, 2002 - v2
Checksum:iAC445C0D92004808
GO
Isoform 11-HSD1B (identifier: P16232-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-26: Missing.

Note: Kidney-specific.
Show »
Length:262
Mass (Da):28,732
Checksum:i22616268BA4DF8C9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti31K → N in CAA71447 (PubMed:10906322).Curated1
Sequence conflicti235E → Q in AAA40886 (PubMed:2808402).Curated1
Sequence conflicti241Missing in AAA40886 (PubMed:2808402).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0126161 – 26Missing in isoform 11-HSD1B. CuratedAdd BLAST26

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05107 mRNA Translation: AAA40886.1
BC078865 mRNA Translation: AAH78865.1
S43333 Genomic DNA Translation: AAB22993.1
Y10420 Genomic DNA Translation: CAA71447.1
M77835 mRNA Translation: AAA40600.1
PIRiA34430 DXRTBH
RefSeqiNP_058776.2, NM_017080.2 [P16232-1]
XP_006250535.1, XM_006250473.3 [P16232-1]
UniGeneiRn.231830
Rn.888

Genome annotation databases

EnsembliENSRNOT00000007870; ENSRNOP00000007870; ENSRNOG00000005861 [P16232-1]
GeneIDi25116
KEGGirno:25116

Keywords - Coding sequence diversityi

Alternative promoter usage

Similar proteinsi

Entry informationi

Entry nameiDHI1_RAT
AccessioniPrimary (citable) accession number: P16232
Secondary accession number(s): O09170, Q6LDH6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: October 10, 2002
Last modified: May 23, 2018
This is version 158 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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