UniProtKB - P16152 (CBR1_HUMAN)
Protein
Carbonyl reductase [NADPH] 1
Gene
CBR1
Organism
Homo sapiens (Human)
Status
Functioni
NADPH-dependent reductase with broad substrate specificity. Catalyzes the reduction of a wide variety of carbonyl compounds including quinones, prostaglandins, menadione, plus various xenobiotics. Catalyzes the reduction of the antitumor anthracyclines doxorubicin and daunorubicin to the cardiotoxic compounds doxorubicinol and daunorubicinol (PubMed:18449627, PubMed:15799708, PubMed:17912391, PubMed:7005231). Can convert prostaglandin E to prostaglandin F2-alpha (By similarity). Can bind glutathione, which explains its higher affinity for glutathione-conjugated substrates. Catalyzes the reduction of S-nitrosoglutathione (PubMed:18826943, PubMed:17344335).By similarity6 Publications
Catalytic activityi
- EC:1.1.1.184
- EC:1.1.1.189By similarityThis reaction proceeds in the backwardBy similarity direction.
- EC:1.1.1.1971 PublicationThis reaction proceeds in the forward1 Publication direction.
- EC:1.1.1.196By similarityThis reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
Activity regulationi
Inhibited by quercetin, rutenin and its derivatives.2 Publications
Kineticsi
- KM=30 µM for S-nitrosoglutathione2 Publications
- KM=22 µM for menadione2 Publications
- KM=309 µM for prostaglandin E22 Publications
- KM=450 µM for prostaglandin E11 Publication
- KM=4 mM for phenylglyoxal1 Publication
- KM=1.6 mM for 4-nitrobenzaldehyde1 Publication
- KM=17 µM for ubiquinone-11 Publication
- KM=173 µM for daunorubicin2 Publications
- KM=247 µM for NADPH2 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 90 | NADP; via carbonyl oxygen3 Publications | 1 | |
Binding sitei | 106 | Glutathione | 1 | |
Binding sitei | 140 | Substrate | 1 | |
Active sitei | 194 | Proton acceptor | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 10 – 34 | NADP3 PublicationsAdd BLAST | 25 | |
Nucleotide bindingi | 63 – 64 | NADP3 Publications | 2 | |
Nucleotide bindingi | 194 – 198 | NADP3 Publications | 5 | |
Nucleotide bindingi | 231 – 233 | NADP3 Publications | 3 |
GO - Molecular functioni
- 15-hydroxyprostaglandin-D dehydrogenase (NADP+) activity Source: UniProtKB-EC
- 15-hydroxyprostaglandin dehydrogenase (NADP+) activity Source: UniProtKB
- carbonyl reductase (NADPH) activity Source: UniProtKB
- oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor Source: UniProtKB
- prostaglandin-E2 9-reductase activity Source: UniProtKB
GO - Biological processi
- cyclooxygenase pathway Source: Reactome
- drug metabolic process Source: UniProtKB
- epithelial cell differentiation Source: UniProtKB
- oxidation-reduction process Source: UniProtKB
- vitamin K metabolic process Source: UniProtKB
Keywordsi
Molecular function | Oxidoreductase |
Ligand | NADP |
Enzyme and pathway databases
BRENDAi | 1.1.1.184, 2681 |
PathwayCommonsi | P16152 |
Reactomei | R-HSA-2162123, Synthesis of Prostaglandins (PG) and Thromboxanes (TX) |
SABIO-RKi | P16152 |
Names & Taxonomyi
Protein namesi | Recommended name: Carbonyl reductase [NADPH] 1Curated (EC:1.1.1.184)Alternative name(s): 15-hydroxyprostaglandin dehydrogenase [NADP(+)] (EC:1.1.1.196By similarity, EC:1.1.1.1971 Publication) 20-beta-hydroxysteroid dehydrogenase NADPH-dependent carbonyl reductase 1 Prostaglandin 9-ketoreductase1 Publication Short name: PG-9-KR1 Publication Prostaglandin-E(2) 9-reductaseBy similarity (EC:1.1.1.189By similarity) Short chain dehydrogenase/reductase family 21C member 1 |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000159228.12 |
HGNCi | HGNC:1548, CBR1 |
MIMi | 114830, gene |
neXtProti | NX_P16152 |
Subcellular locationi
Other locations
- Cytoplasm Curated
Cytosol
- cytosol Source: Reactome
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
- extracellular vesicle Source: UniProtKB
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Organism-specific databases
DisGeNETi | 873 |
OpenTargetsi | ENSG00000159228 |
PharmGKBi | PA26121 |
Miscellaneous databases
Pharosi | P16152, Tchem |
Chemistry databases
ChEMBLi | CHEMBL5586 |
DrugBanki | DB03556, 2-(2-{2-[2-(2-{2-[2-(2-Ethoxy-Ethoxy)-Ethoxy]-Ethoxy}-Ethoxy)-Ethoxy]-Ethoxy}-Ethoxy)-Ethanol, Polyethyleneglycol Peg400 DB04463, 3-(4-Amino-1-Tert-Butyl-1h-Pyrazolo[3,4-D]Pyrimidin-3-Yl)Phenol DB00414, Acetohexamide DB06263, Amrubicin DB11672, Curcumin DB14635, Curcumin sulfate DB12161, Deutetrabenazine DB00997, Doxorubicin DB01039, Fenofibrate DB00502, Haloperidol DB03394, Heptaethylene glycol DB09212, Loxoprofen DB01046, Lubiprostone DB00776, Oxcarbazepine DB04216, Quercetin DB02709, Resveratrol DB01698, Rutin DB05197, Sofalcone DB04844, Tetrabenazine |
DrugCentrali | P16152 |
GuidetoPHARMACOLOGYi | 1383 |
Polymorphism and mutation databases
BioMutai | CBR1 |
DMDMi | 118519 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000054602 | 2 – 277 | Carbonyl reductase [NADPH] 1Add BLAST | 276 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineCombined sources | 1 | |
Modified residuei | 2 | PhosphoserineBy similarity | 1 | |
Modified residuei | 30 | PhosphoserineBy similarity | 1 | |
Modified residuei | 239 | N6-1-carboxyethyl lysine1 Publication | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
CPTACi | CPTAC-329 CPTAC-330 |
EPDi | P16152 |
jPOSTi | P16152 |
MassIVEi | P16152 |
MaxQBi | P16152 |
PaxDbi | P16152 |
PeptideAtlasi | P16152 |
PRIDEi | P16152 |
ProteomicsDBi | 4051 53298 [P16152-1] |
TopDownProteomicsi | P16152-1 [P16152-1] |
2D gel databases
REPRODUCTION-2DPAGEi | IPI00295386 |
UCD-2DPAGEi | P16152 |
PTM databases
iPTMneti | P16152 |
MetOSitei | P16152 |
PhosphoSitePlusi | P16152 |
SwissPalmi | P16152 |
Expressioni
Tissue specificityi
Expressed in kidney (at protein level).1 Publication
Gene expression databases
Bgeei | ENSG00000159228, Expressed in duodenum and 237 other tissues |
ExpressionAtlasi | P16152, baseline and differential |
Genevisiblei | P16152, HS |
Organism-specific databases
HPAi | ENSG00000159228, Tissue enhanced (intestine) |
Interactioni
Subunit structurei
Monomer.
3 PublicationsProtein-protein interaction databases
BioGRIDi | 107319, 53 interactors |
DIPi | DIP-33136N |
IntActi | P16152, 38 interactors |
MINTi | P16152 |
STRINGi | 9606.ENSP00000290349 |
Chemistry databases
BindingDBi | P16152 |
Miscellaneous databases
RNActi | P16152, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P16152 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P16152 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 95 – 97 | Glutathione binding | 3 | |
Regioni | 193 – 194 | Glutathione binding | 2 |
Sequence similaritiesi
Belongs to the short-chain dehydrogenases/reductases (SDR) family.Curated
Phylogenomic databases
eggNOGi | KOG1208, Eukaryota |
GeneTreei | ENSGT00510000046499 |
HOGENOMi | CLU_010194_9_0_1 |
InParanoidi | P16152 |
OMAi | IRVTNAM |
OrthoDBi | 1259665at2759 |
PhylomeDBi | P16152 |
TreeFami | TF329359 |
Family and domain databases
InterProi | View protein in InterPro IPR036291, NAD(P)-bd_dom_sf IPR020904, Sc_DH/Rdtase_CS IPR002347, SDR_fam |
Pfami | View protein in Pfam PF00106, adh_short, 1 hit |
PRINTSi | PR00081, GDHRDH PR00080, SDRFAMILY |
SUPFAMi | SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00061, ADH_SHORT, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: P16152-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSSGIHVALV TGGNKGIGLA IVRDLCRLFS GDVVLTARDV TRGQAAVQQL
60 70 80 90 100
QAEGLSPRFH QLDIDDLQSI RALRDFLRKE YGGLDVLVNN AGIAFKVADP
110 120 130 140 150
TPFHIQAEVT MKTNFFGTRD VCTELLPLIK PQGRVVNVSS IMSVRALKSC
160 170 180 190 200
SPELQQKFRS ETITEEELVG LMNKFVEDTK KGVHQKEGWP SSAYGVTKIG
210 220 230 240 250
VTVLSRIHAR KLSEQRKGDK ILLNACCPGW VRTDMAGPKA TKSPEEGAET
260 270
PVYLALLPPD AEGPHGQFVS EKRVEQW
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA8MTM1 | A8MTM1_HUMAN | Carbonyl reductase [NADPH] 1 | CBR1 | 222 | Annotation score: | ||
E9PQ63 | E9PQ63_HUMAN | Carbonyl reductase [NADPH] 1 | CBR1 | 178 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_059053 | 88 | V → I Reduced affinity for NADPH and reduced activity towards daunorubicin and prostaglandin E2. 2 PublicationsCorresponds to variant dbSNP:rs1143663Ensembl. | 1 | |
Natural variantiVAR_031706 | 131 | P → S1 PublicationCorresponds to variant dbSNP:rs41557318Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_054796 | 133 – 173 | GRVVN…VGLMN → ASCVLSAWSCLSQNPSGGKS KPLAWFTEMSIICRCLTLGP F in isoform 2. 1 PublicationAdd BLAST | 41 | |
Alternative sequenceiVSP_054797 | 174 – 277 | Missing in isoform 2. 1 PublicationAdd BLAST | 104 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J04056 mRNA Translation: AAA52070.1 M62420 Genomic DNA Translation: AAA17881.1 AB003151 Genomic DNA Translation: BAA33498.1 AP000688 Genomic DNA Translation: BAA89424.1 AB124848 mRNA Translation: BAE45940.1 BT019843 mRNA Translation: AAV38646.1 CR541708 mRNA Translation: CAG46509.1 AK294142 mRNA Translation: BAG57468.1 AK314879 mRNA Translation: BAG37394.1 EF141836 Genomic DNA Translation: ABK97430.1 AP001724 Genomic DNA Translation: BAA95508.1 CH471079 Genomic DNA Translation: EAX09754.1 CH471079 Genomic DNA Translation: EAX09755.1 BC002511 mRNA Translation: AAH02511.1 BC015640 mRNA Translation: AAH15640.1 |
CCDSi | CCDS13641.1 [P16152-1] CCDS68202.1 [P16152-2] |
PIRi | A61271, RDHUCB |
RefSeqi | NP_001273718.1, NM_001286789.1 [P16152-2] NP_001748.1, NM_001757.3 [P16152-1] |
Genome annotation databases
Ensembli | ENST00000290349; ENSP00000290349; ENSG00000159228 [P16152-1] ENST00000530908; ENSP00000434613; ENSG00000159228 [P16152-2] |
GeneIDi | 873 |
KEGGi | hsa:873 |
UCSCi | uc002yvb.3, human [P16152-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Web resourcesi
SeattleSNPs |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J04056 mRNA Translation: AAA52070.1 M62420 Genomic DNA Translation: AAA17881.1 AB003151 Genomic DNA Translation: BAA33498.1 AP000688 Genomic DNA Translation: BAA89424.1 AB124848 mRNA Translation: BAE45940.1 BT019843 mRNA Translation: AAV38646.1 CR541708 mRNA Translation: CAG46509.1 AK294142 mRNA Translation: BAG57468.1 AK314879 mRNA Translation: BAG37394.1 EF141836 Genomic DNA Translation: ABK97430.1 AP001724 Genomic DNA Translation: BAA95508.1 CH471079 Genomic DNA Translation: EAX09754.1 CH471079 Genomic DNA Translation: EAX09755.1 BC002511 mRNA Translation: AAH02511.1 BC015640 mRNA Translation: AAH15640.1 |
CCDSi | CCDS13641.1 [P16152-1] CCDS68202.1 [P16152-2] |
PIRi | A61271, RDHUCB |
RefSeqi | NP_001273718.1, NM_001286789.1 [P16152-2] NP_001748.1, NM_001757.3 [P16152-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1WMA | X-ray | 1.24 | A | 2-277 | [»] | |
2PFG | X-ray | 1.54 | A | 2-277 | [»] | |
3BHI | X-ray | 2.27 | A | 2-277 | [»] | |
3BHJ | X-ray | 1.77 | A | 2-277 | [»] | |
3BHM | X-ray | 1.80 | A | 2-277 | [»] | |
4Z3D | X-ray | 1.80 | A/B/C/D | 2-277 | [»] | |
SMRi | P16152 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 107319, 53 interactors |
DIPi | DIP-33136N |
IntActi | P16152, 38 interactors |
MINTi | P16152 |
STRINGi | 9606.ENSP00000290349 |
Chemistry databases
BindingDBi | P16152 |
ChEMBLi | CHEMBL5586 |
DrugBanki | DB03556, 2-(2-{2-[2-(2-{2-[2-(2-Ethoxy-Ethoxy)-Ethoxy]-Ethoxy}-Ethoxy)-Ethoxy]-Ethoxy}-Ethoxy)-Ethanol, Polyethyleneglycol Peg400 DB04463, 3-(4-Amino-1-Tert-Butyl-1h-Pyrazolo[3,4-D]Pyrimidin-3-Yl)Phenol DB00414, Acetohexamide DB06263, Amrubicin DB11672, Curcumin DB14635, Curcumin sulfate DB12161, Deutetrabenazine DB00997, Doxorubicin DB01039, Fenofibrate DB00502, Haloperidol DB03394, Heptaethylene glycol DB09212, Loxoprofen DB01046, Lubiprostone DB00776, Oxcarbazepine DB04216, Quercetin DB02709, Resveratrol DB01698, Rutin DB05197, Sofalcone DB04844, Tetrabenazine |
DrugCentrali | P16152 |
GuidetoPHARMACOLOGYi | 1383 |
PTM databases
iPTMneti | P16152 |
MetOSitei | P16152 |
PhosphoSitePlusi | P16152 |
SwissPalmi | P16152 |
Polymorphism and mutation databases
BioMutai | CBR1 |
DMDMi | 118519 |
2D gel databases
REPRODUCTION-2DPAGEi | IPI00295386 |
UCD-2DPAGEi | P16152 |
Proteomic databases
CPTACi | CPTAC-329 CPTAC-330 |
EPDi | P16152 |
jPOSTi | P16152 |
MassIVEi | P16152 |
MaxQBi | P16152 |
PaxDbi | P16152 |
PeptideAtlasi | P16152 |
PRIDEi | P16152 |
ProteomicsDBi | 4051 53298 [P16152-1] |
TopDownProteomicsi | P16152-1 [P16152-1] |
Protocols and materials databases
Antibodypediai | 8245, 449 antibodies |
DNASUi | 873 |
Genome annotation databases
Ensembli | ENST00000290349; ENSP00000290349; ENSG00000159228 [P16152-1] ENST00000530908; ENSP00000434613; ENSG00000159228 [P16152-2] |
GeneIDi | 873 |
KEGGi | hsa:873 |
UCSCi | uc002yvb.3, human [P16152-1] |
Organism-specific databases
CTDi | 873 |
DisGeNETi | 873 |
EuPathDBi | HostDB:ENSG00000159228.12 |
GeneCardsi | CBR1 |
HGNCi | HGNC:1548, CBR1 |
HPAi | ENSG00000159228, Tissue enhanced (intestine) |
MIMi | 114830, gene |
neXtProti | NX_P16152 |
OpenTargetsi | ENSG00000159228 |
PharmGKBi | PA26121 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1208, Eukaryota |
GeneTreei | ENSGT00510000046499 |
HOGENOMi | CLU_010194_9_0_1 |
InParanoidi | P16152 |
OMAi | IRVTNAM |
OrthoDBi | 1259665at2759 |
PhylomeDBi | P16152 |
TreeFami | TF329359 |
Enzyme and pathway databases
BRENDAi | 1.1.1.184, 2681 |
PathwayCommonsi | P16152 |
Reactomei | R-HSA-2162123, Synthesis of Prostaglandins (PG) and Thromboxanes (TX) |
SABIO-RKi | P16152 |
Miscellaneous databases
BioGRID-ORCSi | 873, 2 hits in 843 CRISPR screens |
ChiTaRSi | CBR1, human |
EvolutionaryTracei | P16152 |
GeneWikii | CBR1 |
GenomeRNAii | 873 |
Pharosi | P16152, Tchem |
PROi | PR:P16152 |
RNActi | P16152, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000159228, Expressed in duodenum and 237 other tissues |
ExpressionAtlasi | P16152, baseline and differential |
Genevisiblei | P16152, HS |
Family and domain databases
InterProi | View protein in InterPro IPR036291, NAD(P)-bd_dom_sf IPR020904, Sc_DH/Rdtase_CS IPR002347, SDR_fam |
Pfami | View protein in Pfam PF00106, adh_short, 1 hit |
PRINTSi | PR00081, GDHRDH PR00080, SDRFAMILY |
SUPFAMi | SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00061, ADH_SHORT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CBR1_HUMAN | |
Accessioni | P16152Primary (citable) accession number: P16152 Secondary accession number(s): B2RBZ7, B4DFK7, Q3LHW8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1990 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 218 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 21
Human chromosome 21: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations