Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Leukosialin

Gene

SPN

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Predominant cell surface sialoprotein of leukocytes which regulates multiple T-cell functions, including T-cell activation, proliferation, differentiation, trafficking and migration. Positively regulates T-cell trafficking to lymph-nodes via its association with ERM proteins (EZR, RDX and MSN) (By similarity). Negatively regulates Th2 cell differentiation and predisposes the differentiation of T-cells towards a Th1 lineage commitment. Promotes the expression of IFN-gamma by T-cells during T-cell receptor (TCR) activation of naive cells and induces the expression of IFN-gamma by CD4+ T-cells and to a lesser extent by CD8+ T-cells (PubMed:18036228). Plays a role in preparing T-cells for cytokine sensing and differentiation into effector cells by inducing the expression of cytokine receptors IFNGR and IL4R, promoting IFNGR and IL4R signaling and by mediating the clustering of IFNGR with TCR (PubMed:24328034). Acts as a major E-selectin ligand responsible for Th17 cell rolling on activated vasculature and recruitment during inflammation. Mediates Th17 cells, but not Th1 cells, adhesion to E-selectin. Acts as a T-cell counter-receptor for SIGLEC1 (By similarity).By similarity2 Publications
CD43 cytoplasmic tail: Protects cells from apoptotic signals, promoting cell survival.By similarity

GO - Molecular functioni

  • heat shock protein binding Source: CAFA
  • Hsp70 protein binding Source: CAFA
  • transmembrane signaling receptor activity Source: ProtInc

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-HSA-202733 Cell surface interactions at the vascular wall
R-HSA-210991 Basigin interactions

Names & Taxonomyi

Protein namesi
Recommended name:
Leukosialin
Alternative name(s):
GPL1151 Publication
Galactoglycoprotein
Short name:
GALGP
Leukocyte sialoglycoprotein
Sialophorin
CD_antigen: CD43
Cleaved into the following chain:
CD43 cytoplasmic tail1 Publication
Short name:
CD43-ct1 Publication
Short name:
CD43ct1 Publication
Gene namesi
Name:SPN
Synonyms:CD43
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

EuPathDBiHostDB:ENSG00000197471.11
HGNCiHGNC:11249 SPN
MIMi182160 gene
neXtProtiNX_P16150

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini20 – 253ExtracellularSequence analysisAdd BLAST234
Transmembranei254 – 276HelicalSequence analysisAdd BLAST23
Topological domaini277 – 400CytoplasmicSequence analysisAdd BLAST124

Keywords - Cellular componenti

Cell projection, Membrane, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi6693
OpenTargetsiENSG00000197471
PharmGKBiPA36079

Polymorphism and mutation databases

BioMutaiSPN
DMDMi126213

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 191 PublicationAdd BLAST19
ChainiPRO_000002158820 – 400LeukosialinAdd BLAST381
ChainiPRO_0000443406277 – 400CD43 cytoplasmic tailBy similarityAdd BLAST124

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi21O-linked (GalNAc...) threonine1 Publication1
Glycosylationi22O-linked (GalNAc...) threonine1 Publication1
Glycosylationi26O-linked (GalNAc...) threonine1 Publication1
Glycosylationi28O-linked (GalNAc...) threonine1 Publication1
Glycosylationi29O-linked (GalNAc...) serine1 Publication1
Glycosylationi35O-linked (GalNAc...) serine1 Publication1
Glycosylationi36O-linked (GalNAc...) threonine1 Publication1
Glycosylationi37O-linked (GalNAc...) serine1 Publication1
Glycosylationi41O-linked (GalNAc...) serine1 Publication1
Glycosylationi42O-linked (GalNAc...) serine1 Publication1
Glycosylationi46O-linked (GalNAc...) threonine1 Publication1
Glycosylationi47O-linked (GalNAc...) threonine1 Publication1
Glycosylationi48O-linked (GalNAc...) serine1 Publication1
Glycosylationi50O-linked (GalNAc...) threonine1 Publication1
Glycosylationi58O-linked (GalNAc...) threonine1 Publication1
Glycosylationi69O-linked (GalNAc...) threonine1 Publication1
Glycosylationi99O-linked (GalNAc...) serine1 Publication1
Glycosylationi103O-linked (GalNAc...) serine1 Publication1
Glycosylationi109O-linked (GalNAc...) threonine1 Publication1
Glycosylationi113O-linked (GalNAc...) threonine1 Publication1
Glycosylationi114O-linked (GalNAc...) serine1 Publication1
Glycosylationi136O-linked (GalNAc...) threonine1 Publication1
Glycosylationi137O-linked (GalNAc...) threonine1 Publication1
Glycosylationi173O-linked (GalNAc...) threonine1 Publication1
Glycosylationi178O-linked (GalNAc...) threonine1 Publication1
Glycosylationi239N-linked (GlcNAc...) asparagine1 Publication1
Modified residuei291PhosphoserineCombined sources1 Publication1
Modified residuei336PhosphoserineCombined sources1
Modified residuei341PhosphothreonineCombined sources1
Modified residuei351PhosphoserineCombined sources1 Publication1
Modified residuei355Phosphoserine; by PKC/PRKCQCombined sources1
Modified residuei368PhosphoserineCombined sources1
Modified residuei379PhosphoserineBy similarity1

Post-translational modificationi

Glycosylated; has a high content of sialic acid and O-linked carbohydrate structures.1 Publication
Phosphorylation at Ser-355 is regulated by chemokines, requires its association with ERM proteins (EZR, RDX and MSN) and is essential for its function in the regulation of T-cell trafficking to lymph nodes.By similarity
Has a high content of sialic acid and O-linked carbohydrate structures.
Cleavage by CTSG releases its extracellular domain and triggers its intramembrane proteolysis by gamma-secretase releasing the CD43 cytoplasmic tail chain (CD43-ct) which translocates to the nucleus.2 Publications
CD43 cytoplasmic tail: Sumoylated.

Keywords - PTMi

Glycoprotein, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP16150
MaxQBiP16150
PaxDbiP16150
PeptideAtlasiP16150
PRIDEiP16150
ProteomicsDBi53297

PTM databases

GlyConnecti340
iPTMnetiP16150
PhosphoSitePlusiP16150
UniCarbKBiP16150

Miscellaneous databases

PMAP-CutDBiP16150

Expressioni

Tissue specificityi

Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas.

Gene expression databases

BgeeiENSG00000197471 Expressed in 208 organ(s), highest expression level in buccal mucosa cell
CleanExiHS_SPN
ExpressionAtlasiP16150 baseline and differential
GenevisibleiP16150 HS

Organism-specific databases

HPAiCAB002666
HPA055244

Interactioni

Subunit structurei

Interacts with HIPK2 via the cytoplasmic domain. Interacts with SIGLEC1, RDX, EZR and MSN.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi112571, 13 interactors
IntActiP16150, 9 interactors
STRINGi9606.ENSP00000353238

Structurei

3D structure databases

ProteinModelPortaliP16150
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni278 – 308Required for interaction with EZR, MSN and RDX and for co-localization to microvilliBy similarityAdd BLAST31

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410J161 Eukaryota
ENOG410YXQE LUCA
GeneTreeiENSGT00390000017626
HOGENOMiHOG000294199
HOVERGENiHBG006258
InParanoidiP16150
KOiK06477
OMAiTGPPVTM
OrthoDBiEOG091G0MEV
PhylomeDBiP16150
TreeFamiTF337688

Family and domain databases

InterProiView protein in InterPro
IPR038829 Leukosialin
PANTHERiPTHR35265 PTHR35265, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P16150-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MATLLLLLGV LVVSPDALGS TTAVQTPTSG EPLVSTSEPL SSKMYTTSIT
60 70 80 90 100
SDPKADSTGD QTSALPPSTS INEGSPLWTS IGASTGSPLP EPTTYQEVSI
110 120 130 140 150
KMSSVPQETP HATSHPAVPI TANSLGSHTV TGGTITTNSP ETSSRTSGAP
160 170 180 190 200
VTTAASSLET SRGTSGPPLT MATVSLETSK GTSGPPVTMA TDSLETSTGT
210 220 230 240 250
TGPPVTMTTG SLEPSSGASG PQVSSVKLST MMSPTTSTNA STVPFRNPDE
260 270 280 290 300
NSRGMLPVAV LVALLAVIVL VALLLLWRRR QKRRTGALVL SRGGKRNGVV
310 320 330 340 350
DAWAGPAQVP EEGAVTVTVG GSGGDKGSGF PDGEGSSRRP TLTTFFGRRK
360 370 380 390 400
SRQGSLAMEE LKSGSGPSLK GEEEPLVASE DGAVDAPAPD EPEGGDGAAP
Length:400
Mass (Da):40,322
Last modified:April 1, 1990 - v1
Checksum:iC9C9AB8435D5E1FE
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9JUK7C9JUK7_HUMAN
Leukosialin
SPN
355Annotation score:

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05109122T → I. Corresponds to variant dbSNP:rs2229653Ensembl.1
Natural variantiVAR_05109293T → A. Corresponds to variant dbSNP:rs2229654Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04168 mRNA Translation: AAA59510.1
J04536 mRNA Translation: AAB59540.1
X52075 Genomic DNA Translation: CAA36294.1
M61827 Genomic DNA Translation: AAA51949.1
AK292626 mRNA Translation: BAF85315.1
AK313750 mRNA Translation: BAG36490.1
CH471238 Genomic DNA Translation: EAW80015.1
BC012350 mRNA Translation: AAH12350.1
CCDSiCCDS10650.1
PIRiA39822
RefSeqiNP_001025459.1, NM_001030288.2
NP_003114.1, NM_003123.4
UniGeneiHs.632188

Genome annotation databases

EnsembliENST00000360121; ENSP00000353238; ENSG00000197471
ENST00000395389; ENSP00000378787; ENSG00000197471
ENST00000563039; ENSP00000455266; ENSG00000197471
GeneIDi6693
KEGGihsa:6693
UCSCiuc002dtm.5 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04168 mRNA Translation: AAA59510.1
J04536 mRNA Translation: AAB59540.1
X52075 Genomic DNA Translation: CAA36294.1
M61827 Genomic DNA Translation: AAA51949.1
AK292626 mRNA Translation: BAF85315.1
AK313750 mRNA Translation: BAG36490.1
CH471238 Genomic DNA Translation: EAW80015.1
BC012350 mRNA Translation: AAH12350.1
CCDSiCCDS10650.1
PIRiA39822
RefSeqiNP_001025459.1, NM_001030288.2
NP_003114.1, NM_003123.4
UniGeneiHs.632188

3D structure databases

ProteinModelPortaliP16150
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112571, 13 interactors
IntActiP16150, 9 interactors
STRINGi9606.ENSP00000353238

PTM databases

GlyConnecti340
iPTMnetiP16150
PhosphoSitePlusiP16150
UniCarbKBiP16150

Polymorphism and mutation databases

BioMutaiSPN
DMDMi126213

Proteomic databases

EPDiP16150
MaxQBiP16150
PaxDbiP16150
PeptideAtlasiP16150
PRIDEiP16150
ProteomicsDBi53297

Protocols and materials databases

DNASUi6693
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000360121; ENSP00000353238; ENSG00000197471
ENST00000395389; ENSP00000378787; ENSG00000197471
ENST00000563039; ENSP00000455266; ENSG00000197471
GeneIDi6693
KEGGihsa:6693
UCSCiuc002dtm.5 human

Organism-specific databases

CTDi6693
DisGeNETi6693
EuPathDBiHostDB:ENSG00000197471.11
GeneCardsiSPN
HGNCiHGNC:11249 SPN
HPAiCAB002666
HPA055244
MIMi182160 gene
neXtProtiNX_P16150
OpenTargetsiENSG00000197471
PharmGKBiPA36079
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410J161 Eukaryota
ENOG410YXQE LUCA
GeneTreeiENSGT00390000017626
HOGENOMiHOG000294199
HOVERGENiHBG006258
InParanoidiP16150
KOiK06477
OMAiTGPPVTM
OrthoDBiEOG091G0MEV
PhylomeDBiP16150
TreeFamiTF337688

Enzyme and pathway databases

ReactomeiR-HSA-202733 Cell surface interactions at the vascular wall
R-HSA-210991 Basigin interactions

Miscellaneous databases

GeneWikiiCD43
PMAP-CutDBiP16150
PROiPR:P16150
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000197471 Expressed in 208 organ(s), highest expression level in buccal mucosa cell
CleanExiHS_SPN
ExpressionAtlasiP16150 baseline and differential
GenevisibleiP16150 HS

Family and domain databases

InterProiView protein in InterPro
IPR038829 Leukosialin
PANTHERiPTHR35265 PTHR35265, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiLEUK_HUMAN
AccessioniPrimary (citable) accession number: P16150
Secondary accession number(s): A8K9B1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: September 12, 2018
This is version 170 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  2. Human cell differentiation molecules
    CD nomenclature of surface proteins of human leucocytes and list of entries
  3. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again