UniProtKB - P16150 (LEUK_HUMAN)
Protein
Leukosialin
Gene
SPN
Organism
Homo sapiens (Human)
Status
Functioni
Predominant cell surface sialoprotein of leukocytes which regulates multiple T-cell functions, including T-cell activation, proliferation, differentiation, trafficking and migration. Positively regulates T-cell trafficking to lymph-nodes via its association with ERM proteins (EZR, RDX and MSN) (By similarity). Negatively regulates Th2 cell differentiation and predisposes the differentiation of T-cells towards a Th1 lineage commitment. Promotes the expression of IFN-gamma by T-cells during T-cell receptor (TCR) activation of naive cells and induces the expression of IFN-gamma by CD4+ T-cells and to a lesser extent by CD8+ T-cells (PubMed:18036228). Plays a role in preparing T-cells for cytokine sensing and differentiation into effector cells by inducing the expression of cytokine receptors IFNGR and IL4R, promoting IFNGR and IL4R signaling and by mediating the clustering of IFNGR with TCR (PubMed:24328034). Acts as a major E-selectin ligand responsible for Th17 cell rolling on activated vasculature and recruitment during inflammation. Mediates Th17 cells, but not Th1 cells, adhesion to E-selectin. Acts as a T-cell counter-receptor for SIGLEC1 (By similarity).By similarity2 Publications
Protects cells from apoptotic signals, promoting cell survival.By similarity
GO - Molecular functioni
- heat shock protein binding Source: CAFA
- Hsp70 protein binding Source: CAFA
- transmembrane signaling receptor activity Source: ProtInc
GO - Biological processi
- cell surface receptor signaling pathway Source: GO_Central
- cellular defense response Source: ProtInc
- chemotaxis Source: ProtInc
- defense response to bacterium Source: MGI
- establishment or maintenance of cell polarity Source: ProtInc
- immune response Source: ProtInc
- leukocyte migration Source: Reactome
- leukocyte tethering or rolling Source: UniProtKB
- negative regulation of cell adhesion Source: ProtInc
- negative regulation of T cell proliferation Source: UniProtKB
- positive regulation of T cell migration Source: UniProtKB
- positive regulation of tumor necrosis factor production Source: MGI
- regulation of immune response Source: GO_Central
- regulation of T cell migration Source: UniProtKB
- signal transduction Source: ProtInc
- T-helper 1 cell lineage commitment Source: UniProtKB
Enzyme and pathway databases
PathwayCommonsi | P16150 |
Reactomei | R-HSA-202733, Cell surface interactions at the vascular wall R-HSA-210991, Basigin interactions |
Names & Taxonomyi
Protein namesi | Recommended name: LeukosialinAlternative name(s): GPL1151 Publication Galactoglycoprotein Short name: GALGP Leukocyte sialoglycoprotein Sialophorin CD_antigen: CD43 Cleaved into the following chain: |
Gene namesi | Name:SPN Synonyms:CD43 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:11249, SPN |
MIMi | 182160, gene |
neXtProti | NX_P16150 |
VEuPathDBi | HostDB:ENSG00000197471.11 |
Subcellular locationi
Other locations
- Membrane Sequence analysis; Single-pass type I membrane protein Sequence analysis
- microvillus By similarity
- uropodium By similarity
Note: Localizes to the uropodium and microvilli via its interaction with ERM proteins (EZR, RDX and MSN).By similarity
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
- extracellular space Source: MGI
Nucleus
- PML body Source: UniProtKB-SubCell
Plasma Membrane
- external side of plasma membrane Source: GO_Central
- integral component of plasma membrane Source: ProtInc
- plasma membrane Source: Reactome
Other locations
- cell surface Source: UniProtKB
- membrane Source: UniProtKB
- microvillus Source: UniProtKB
- uropod Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 20 – 253 | ExtracellularSequence analysisAdd BLAST | 234 | |
Transmembranei | 254 – 276 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 277 – 400 | CytoplasmicSequence analysisAdd BLAST | 124 |
Keywords - Cellular componenti
Cell projection, Membrane, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 6693 |
OpenTargetsi | ENSG00000197471 |
PharmGKBi | PA36079 |
Miscellaneous databases
Pharosi | P16150, Tbio |
Genetic variation databases
BioMutai | SPN |
DMDMi | 126213 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 19 | 1 PublicationAdd BLAST | 19 | |
ChainiPRO_0000021588 | 20 – 400 | LeukosialinAdd BLAST | 381 | |
ChainiPRO_0000443406 | 277 – 400 | CD43 cytoplasmic tailBy similarityAdd BLAST | 124 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 21 | O-linked (GalNAc...) threonine1 Publication | 1 | |
Glycosylationi | 22 | O-linked (GalNAc...) threonine1 Publication | 1 | |
Glycosylationi | 26 | O-linked (GalNAc...) threonine1 Publication | 1 | |
Glycosylationi | 28 | O-linked (GalNAc...) threonine1 Publication | 1 | |
Glycosylationi | 29 | O-linked (GalNAc...) serine1 Publication | 1 | |
Glycosylationi | 35 | O-linked (GalNAc...) serine1 Publication | 1 | |
Glycosylationi | 36 | O-linked (GalNAc...) threonine1 Publication | 1 | |
Glycosylationi | 37 | O-linked (GalNAc...) serine1 Publication | 1 | |
Glycosylationi | 41 | O-linked (GalNAc...) serine1 Publication | 1 | |
Glycosylationi | 42 | O-linked (GalNAc...) serine1 Publication | 1 | |
Glycosylationi | 46 | O-linked (GalNAc...) threonine1 Publication | 1 | |
Glycosylationi | 47 | O-linked (GalNAc...) threonine1 Publication | 1 | |
Glycosylationi | 48 | O-linked (GalNAc...) serine1 Publication | 1 | |
Glycosylationi | 50 | O-linked (GalNAc...) threonine1 Publication | 1 | |
Glycosylationi | 58 | O-linked (GalNAc...) threonine1 Publication | 1 | |
Glycosylationi | 69 | O-linked (GalNAc...) threonine1 Publication | 1 | |
Glycosylationi | 99 | O-linked (GalNAc...) serine1 Publication | 1 | |
Glycosylationi | 103 | O-linked (GalNAc...) serine1 Publication | 1 | |
Glycosylationi | 109 | O-linked (GalNAc...) threonine1 Publication | 1 | |
Glycosylationi | 113 | O-linked (GalNAc...) threonine1 Publication | 1 | |
Glycosylationi | 114 | O-linked (GalNAc...) serine1 Publication | 1 | |
Glycosylationi | 136 | O-linked (GalNAc...) threonine1 Publication | 1 | |
Glycosylationi | 137 | O-linked (GalNAc...) threonine1 Publication | 1 | |
Glycosylationi | 173 | O-linked (GalNAc...) threonine1 Publication | 1 | |
Glycosylationi | 178 | O-linked (GalNAc...) threonine1 Publication | 1 | |
Glycosylationi | 239 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Modified residuei | 291 | PhosphoserineCombined sources1 Publication | 1 | |
Modified residuei | 336 | PhosphoserineCombined sources | 1 | |
Modified residuei | 341 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 351 | PhosphoserineCombined sources1 Publication | 1 | |
Modified residuei | 355 | Phosphoserine; by PKC/PRKCQCombined sources | 1 | |
Modified residuei | 368 | PhosphoserineCombined sources | 1 | |
Modified residuei | 379 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Glycosylated; has a high content of sialic acid and O-linked carbohydrate structures.1 Publication
Phosphorylation at Ser-355 is regulated by chemokines, requires its association with ERM proteins (EZR, RDX and MSN) and is essential for its function in the regulation of T-cell trafficking to lymph nodes.By similarity
Has a high content of sialic acid and O-linked carbohydrate structures.
Cleavage by CTSG releases its extracellular domain and triggers its intramembrane proteolysis by gamma-secretase releasing the CD43 cytoplasmic tail chain (CD43-ct) which translocates to the nucleus.2 Publications
Sumoylated.
Keywords - PTMi
Glycoprotein, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | P16150 |
jPOSTi | P16150 |
MassIVEi | P16150 |
MaxQBi | P16150 |
PaxDbi | P16150 |
PeptideAtlasi | P16150 |
PRIDEi | P16150 |
ProteomicsDBi | 53297 |
PTM databases
GlyConnecti | 340, 11 O-Linked glycans |
GlyGeni | P16150, 28 sites, 8 O-linked glycans (27 sites) |
iPTMneti | P16150 |
PhosphoSitePlusi | P16150 |
Expressioni
Tissue specificityi
Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas.
Gene expression databases
Bgeei | ENSG00000197471, Expressed in buccal mucosa cell and 219 other tissues |
ExpressionAtlasi | P16150, baseline and differential |
Genevisiblei | P16150, HS |
Organism-specific databases
HPAi | ENSG00000197471, Group enriched (blood, bone marrow, lung, lymphoid tissue) |
Interactioni
Subunit structurei
Interacts with HIPK2 via the cytoplasmic domain.
Interacts with SIGLEC1, RDX, EZR and MSN.
By similarityBinary interactionsi
Hide detailsP16150
GO - Molecular functioni
- heat shock protein binding Source: CAFA
- Hsp70 protein binding Source: CAFA
Protein-protein interaction databases
BioGRIDi | 112571, 22 interactors |
CORUMi | P16150 |
IntActi | P16150, 12 interactors |
STRINGi | 9606.ENSP00000353238 |
Miscellaneous databases
RNActi | P16150, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 278 – 308 | Required for interaction with EZR, MSN and RDX and for co-localization to microvilliBy similarityAdd BLAST | 31 |
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502SBHY, Eukaryota |
GeneTreei | ENSGT00390000017626 |
HOGENOMi | CLU_038831_0_1_1 |
InParanoidi | P16150 |
OMAi | RRKSHQG |
OrthoDBi | 1178463at2759 |
PhylomeDBi | P16150 |
TreeFami | TF337688 |
Family and domain databases
InterProi | View protein in InterPro IPR038829, Leukosialin |
PANTHERi | PTHR35265, PTHR35265, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P16150-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MATLLLLLGV LVVSPDALGS TTAVQTPTSG EPLVSTSEPL SSKMYTTSIT
60 70 80 90 100
SDPKADSTGD QTSALPPSTS INEGSPLWTS IGASTGSPLP EPTTYQEVSI
110 120 130 140 150
KMSSVPQETP HATSHPAVPI TANSLGSHTV TGGTITTNSP ETSSRTSGAP
160 170 180 190 200
VTTAASSLET SRGTSGPPLT MATVSLETSK GTSGPPVTMA TDSLETSTGT
210 220 230 240 250
TGPPVTMTTG SLEPSSGASG PQVSSVKLST MMSPTTSTNA STVPFRNPDE
260 270 280 290 300
NSRGMLPVAV LVALLAVIVL VALLLLWRRR QKRRTGALVL SRGGKRNGVV
310 320 330 340 350
DAWAGPAQVP EEGAVTVTVG GSGGDKGSGF PDGEGSSRRP TLTTFFGRRK
360 370 380 390 400
SRQGSLAMEE LKSGSGPSLK GEEEPLVASE DGAVDAPAPD EPEGGDGAAP
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketC9JUK7 | C9JUK7_HUMAN | Leukosialin | SPN | 355 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_051091 | 22 | T → I. Corresponds to variant dbSNP:rs2229653EnsemblClinVar. | 1 | |
Natural variantiVAR_051092 | 93 | T → A. Corresponds to variant dbSNP:rs2229654EnsemblClinVar. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J04168 mRNA Translation: AAA59510.1 J04536 mRNA Translation: AAB59540.1 X52075 Genomic DNA Translation: CAA36294.1 M61827 Genomic DNA Translation: AAA51949.1 AK292626 mRNA Translation: BAF85315.1 AK313750 mRNA Translation: BAG36490.1 CH471238 Genomic DNA Translation: EAW80015.1 BC012350 mRNA Translation: AAH12350.1 |
CCDSi | CCDS10650.1 |
PIRi | A39822 |
RefSeqi | NP_001025459.1, NM_001030288.2 NP_003114.1, NM_003123.4 |
Genome annotation databases
Ensembli | ENST00000360121; ENSP00000353238; ENSG00000197471 ENST00000395389; ENSP00000378787; ENSG00000197471 ENST00000563039; ENSP00000455266; ENSG00000197471 ENST00000652691; ENSP00000498852; ENSG00000197471 |
GeneIDi | 6693 |
KEGGi | hsa:6693 |
UCSCi | uc002dtm.5, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J04168 mRNA Translation: AAA59510.1 J04536 mRNA Translation: AAB59540.1 X52075 Genomic DNA Translation: CAA36294.1 M61827 Genomic DNA Translation: AAA51949.1 AK292626 mRNA Translation: BAF85315.1 AK313750 mRNA Translation: BAG36490.1 CH471238 Genomic DNA Translation: EAW80015.1 BC012350 mRNA Translation: AAH12350.1 |
CCDSi | CCDS10650.1 |
PIRi | A39822 |
RefSeqi | NP_001025459.1, NM_001030288.2 NP_003114.1, NM_003123.4 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 112571, 22 interactors |
CORUMi | P16150 |
IntActi | P16150, 12 interactors |
STRINGi | 9606.ENSP00000353238 |
PTM databases
GlyConnecti | 340, 11 O-Linked glycans |
GlyGeni | P16150, 28 sites, 8 O-linked glycans (27 sites) |
iPTMneti | P16150 |
PhosphoSitePlusi | P16150 |
Genetic variation databases
BioMutai | SPN |
DMDMi | 126213 |
Proteomic databases
EPDi | P16150 |
jPOSTi | P16150 |
MassIVEi | P16150 |
MaxQBi | P16150 |
PaxDbi | P16150 |
PeptideAtlasi | P16150 |
PRIDEi | P16150 |
ProteomicsDBi | 53297 |
Protocols and materials databases
Antibodypediai | 3627, 2147 antibodies |
DNASUi | 6693 |
Genome annotation databases
Ensembli | ENST00000360121; ENSP00000353238; ENSG00000197471 ENST00000395389; ENSP00000378787; ENSG00000197471 ENST00000563039; ENSP00000455266; ENSG00000197471 ENST00000652691; ENSP00000498852; ENSG00000197471 |
GeneIDi | 6693 |
KEGGi | hsa:6693 |
UCSCi | uc002dtm.5, human |
Organism-specific databases
CTDi | 6693 |
DisGeNETi | 6693 |
GeneCardsi | SPN |
HGNCi | HGNC:11249, SPN |
HPAi | ENSG00000197471, Group enriched (blood, bone marrow, lung, lymphoid tissue) |
MIMi | 182160, gene |
neXtProti | NX_P16150 |
OpenTargetsi | ENSG00000197471 |
PharmGKBi | PA36079 |
VEuPathDBi | HostDB:ENSG00000197471.11 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502SBHY, Eukaryota |
GeneTreei | ENSGT00390000017626 |
HOGENOMi | CLU_038831_0_1_1 |
InParanoidi | P16150 |
OMAi | RRKSHQG |
OrthoDBi | 1178463at2759 |
PhylomeDBi | P16150 |
TreeFami | TF337688 |
Enzyme and pathway databases
PathwayCommonsi | P16150 |
Reactomei | R-HSA-202733, Cell surface interactions at the vascular wall R-HSA-210991, Basigin interactions |
Miscellaneous databases
BioGRID-ORCSi | 6693, 9 hits in 989 CRISPR screens |
ChiTaRSi | SPN, human |
GeneWikii | CD43 |
GenomeRNAii | 6693 |
Pharosi | P16150, Tbio |
PROi | PR:P16150 |
RNActi | P16150, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000197471, Expressed in buccal mucosa cell and 219 other tissues |
ExpressionAtlasi | P16150, baseline and differential |
Genevisiblei | P16150, HS |
Family and domain databases
InterProi | View protein in InterPro IPR038829, Leukosialin |
PANTHERi | PTHR35265, PTHR35265, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LEUK_HUMAN | |
Accessioni | P16150Primary (citable) accession number: P16150 Secondary accession number(s): A8K9B1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1990 |
Last sequence update: | April 1, 1990 | |
Last modified: | April 7, 2021 | |
This is version 187 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Human cell differentiation molecules
CD nomenclature of surface proteins of human leucocytes and list of entries - Human chromosome 16
Human chromosome 16: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot