UniProtKB - P16120 (THRC_YEAST)
Protein
Threonine synthase
Gene
THR4
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Catalyzes the gamma-elimination of phosphate from L-phosphohomoserine and the beta-addition of water to produce L-threonine.By similarity
Miscellaneous
Present with 26000 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- EC:4.2.3.1
Cofactori
pyridoxal 5'-phosphateBy similarity
: L-threonine biosynthesis Pathwayi
This protein is involved in step 5 of the subpathway that synthesizes L-threonine from L-aspartate.Proteins known to be involved in the 5 steps of the subpathway in this organism are:
- Aspartokinase (HOM3)
- Aspartate-semialdehyde dehydrogenase (HOM2)
- Homoserine dehydrogenase (HOM6)
- Homoserine kinase (THR1)
- Threonine synthase (THR4)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-threonine from L-aspartate, the pathway L-threonine biosynthesis and in Amino-acid biosynthesis.
GO - Molecular functioni
- pyridoxal phosphate binding Source: InterPro
- threonine synthase activity Source: SGD
GO - Biological processi
- threonine biosynthetic process Source: SGD
Keywordsi
Molecular function | Lyase |
Biological process | Amino-acid biosynthesis, Threonine biosynthesis |
Ligand | Pyridoxal phosphate |
Enzyme and pathway databases
BioCyci | YEAST:YCR053W-MONOMER |
UniPathwayi | UPA00050;UER00065 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:THR4 Ordered Locus Names:YCR053W ORF Names:YCR53W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000000649, THR4 |
VEuPathDBi | FungiDB:YCR053W |
Subcellular locationi
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000185645 | 1 – 514 | Threonine synthaseAdd BLAST | 514 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 124 | N6-(pyridoxal phosphate)lysineBy similarity | 1 | |
Modified residuei | 467 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P16120 |
PaxDbi | P16120 |
PRIDEi | P16120 |
TopDownProteomicsi | P16120 |
PTM databases
iPTMneti | P16120 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 31033, 772 interactors |
DIPi | DIP-6522N |
IntActi | P16120, 5 interactors |
MINTi | P16120 |
STRINGi | 4932.YCR053W |
Miscellaneous databases
RNActi | P16120, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P16120 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P16120 |
Family & Domainsi
Sequence similaritiesi
Belongs to the threonine synthase family.Curated
Phylogenomic databases
eggNOGi | KOG2616, Eukaryota |
GeneTreei | ENSGT00940000161144 |
HOGENOMi | CLU_015170_1_0_1 |
InParanoidi | P16120 |
OMAi | TYYFYSY |
Family and domain databases
Gene3Di | 3.40.50.1100, 2 hits 3.90.1380.10, 1 hit |
InterProi | View protein in InterPro IPR001926, PLP-dep IPR000634, Ser/Thr_deHydtase_PyrdxlP-BS IPR029144, Thr_synth_N IPR037158, Thr_synth_N_sf IPR004450, Thr_synthase-like IPR036052, Trypto_synt_PLP_dependent |
Pfami | View protein in Pfam PF00291, PALP, 1 hit PF14821, Thr_synth_N, 1 hit |
SUPFAMi | SSF53686, SSF53686, 1 hit |
TIGRFAMsi | TIGR00260, thrC, 1 hit |
PROSITEi | View protein in PROSITE PS00165, DEHYDRATASE_SER_THR, 1 hit |
i Sequence
Sequence statusi: Complete.
P16120-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPNASQVYRS TRSSSPKTIS FEEAIIQGLA TDGGLFIPPT IPQVDQATLF
60 70 80 90 100
NDWSKLSFQD LAFAIMRLYI AQEEIPDADL KDLIKRSYST FRSDEVTPLV
110 120 130 140 150
QNVTGDKENL HILELFHGPT YAFKDVALQF VGNLFEYFLQ RTNANLPEGE
160 170 180 190 200
KKQITVVGAT SGDTGSAAIY GLRGKKDVSV FILYPTGRIS PIQEEQMTTV
210 220 230 240 250
PDENVQTLSV TGTFDNCQDI VKAIFGDKEF NSKHNVGAVN SINWARILAQ
260 270 280 290 300
MTYYFYSFFQ ATNGKDSKKV KFVVPSGNFG DILAGYFAKK MGLPIEKLAI
310 320 330 340 350
ATNENDILDR FLKSGLYERS DKVAATLSPA MDILISSNFE RLLWYLAREY
360 370 380 390 400
LANGDDLKAG EIVNNWFQEL KTNGKFQVDK SIIEGASKDF TSERVSNEET
410 420 430 440 450
SETIKKIYES SVNPKHYILD PHTAVGVCAT ERLIAKDNDK SIQYISLSTA
460 470 480 490 500
HPAKFADAVN NALSGFSNYS FEKDVLPEEL KKLSTLKKKL KFIERADVEL
510
VKNAIEEELA KMKL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 176 | K → R in AAU09679 (PubMed:17322287).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X17256 Genomic DNA Translation: CAA35157.1 X59720 Genomic DNA Translation: CAA42284.1 AY723762 Genomic DNA Translation: AAU09679.1 BK006937 Genomic DNA Translation: DAA07530.1 |
PIRi | S22836, SYBSR |
RefSeqi | NP_009982.1, NM_001178767.1 |
Genome annotation databases
EnsemblFungii | YCR053W_mRNA; YCR053W; YCR053W |
GeneIDi | 850420 |
KEGGi | sce:YCR053W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X17256 Genomic DNA Translation: CAA35157.1 X59720 Genomic DNA Translation: CAA42284.1 AY723762 Genomic DNA Translation: AAU09679.1 BK006937 Genomic DNA Translation: DAA07530.1 |
PIRi | S22836, SYBSR |
RefSeqi | NP_009982.1, NM_001178767.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1KL7 | X-ray | 2.70 | A/B | 1-514 | [»] | |
SMRi | P16120 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 31033, 772 interactors |
DIPi | DIP-6522N |
IntActi | P16120, 5 interactors |
MINTi | P16120 |
STRINGi | 4932.YCR053W |
PTM databases
iPTMneti | P16120 |
Proteomic databases
MaxQBi | P16120 |
PaxDbi | P16120 |
PRIDEi | P16120 |
TopDownProteomicsi | P16120 |
Genome annotation databases
EnsemblFungii | YCR053W_mRNA; YCR053W; YCR053W |
GeneIDi | 850420 |
KEGGi | sce:YCR053W |
Organism-specific databases
SGDi | S000000649, THR4 |
VEuPathDBi | FungiDB:YCR053W |
Phylogenomic databases
eggNOGi | KOG2616, Eukaryota |
GeneTreei | ENSGT00940000161144 |
HOGENOMi | CLU_015170_1_0_1 |
InParanoidi | P16120 |
OMAi | TYYFYSY |
Enzyme and pathway databases
UniPathwayi | UPA00050;UER00065 |
BioCyci | YEAST:YCR053W-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P16120 |
PROi | PR:P16120 |
RNActi | P16120, protein |
Family and domain databases
Gene3Di | 3.40.50.1100, 2 hits 3.90.1380.10, 1 hit |
InterProi | View protein in InterPro IPR001926, PLP-dep IPR000634, Ser/Thr_deHydtase_PyrdxlP-BS IPR029144, Thr_synth_N IPR037158, Thr_synth_N_sf IPR004450, Thr_synthase-like IPR036052, Trypto_synt_PLP_dependent |
Pfami | View protein in Pfam PF00291, PALP, 1 hit PF14821, Thr_synth_N, 1 hit |
SUPFAMi | SSF53686, SSF53686, 1 hit |
TIGRFAMsi | TIGR00260, thrC, 1 hit |
PROSITEi | View protein in PROSITE PS00165, DEHYDRATASE_SER_THR, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | THRC_YEAST | |
Accessioni | P16120Primary (citable) accession number: P16120 Secondary accession number(s): D6VR61, E9P943 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1990 |
Last sequence update: | April 1, 1990 | |
Last modified: | April 7, 2021 | |
This is version 185 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome III
Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families