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Protein

L-lactate dehydrogenase

Gene

ldh

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of lactate to pyruvate. It is stereospecific for L+-lactate.UniRule annotation1 Publication

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.UniRule annotation1 Publication

Activity regulationi

Allosterically activated by fructose 1,6-bisphosphate (FBP). Inactivated by Mn2+, Co2+, Cd2+ and Zn2+.1 Publication

Kineticsi

  1. KM=30 µM for NADH (at 55 degrees Celsius and in the absence of fructose 1,6-bisphosphate (FBP))1 Publication
  2. KM=60 µM for pyruvate (at 55 degrees Celsius and in the presence of fructose 1,6-bisphosphate (FBP))1 Publication
  3. KM=90 µM for NAD+ (at 55 degrees Celsius and in the absence of fructose 1,6-bisphosphate (FBP))1 Publication
  4. KM=3.7 mM for pyruvate (at 55 degrees Celsius and in the absence of fructose 1,6-bisphosphate (FBP))1 Publication
  5. KM=25 mM for L+-lactate (at 55 degrees Celsius and in the presence of fructose 1,6-bisphosphate (FBP))1 Publication
  6. KM=410 mM for L+-lactate (at 55 degrees Celsius and in the absence of fructose 1,6-bisphosphate (FBP))1 Publication

    pH dependencei

    Optimum pH is 7.1 Publication

    Temperature dependencei

    Long-term stability up to 80 degrees Celsius. Fructose 1,6-bisphosphate (FBP) increases the thermal stability at 90 degrees Celsius (pH 6.0).1 Publication

    Pathwayi: pyruvate fermentation to lactate

    This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.UniRule annotation
    Proteins known to be involved in this subpathway in this organism are:
    1. L-lactate dehydrogenase (ldh), L-lactate dehydrogenase (ldh)
    This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei32NADUniRule annotationCombined sources1 Publication1
    Binding sitei37NADUniRule annotation1
    Binding sitei62NADUniRule annotation1
    Binding sitei79SubstrateUniRule annotation1
    Binding sitei85SubstrateUniRule annotation1
    Binding sitei140NADUniRule annotation1
    Binding sitei150Allosteric activatorUniRule annotation1
    Binding sitei165Allosteric activatorUniRule annotationCombined sources1 Publication1
    Active sitei172Proton acceptorUniRule annotation1 Publication1
    Binding sitei226SubstrateUniRule annotation1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi10 – 11NADCombined sources1 Publication2
    Nucleotide bindingi76 – 77NADUniRule annotation2
    Nucleotide bindingi115 – 117NADUniRule annotation3

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionAllosteric enzyme, Oxidoreductase
    LigandNAD

    Enzyme and pathway databases

    UniPathwayi
    UPA00554;UER00611

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    L-lactate dehydrogenase1 PublicationUniRule annotation (EC:1.1.1.27UniRule annotation1 Publication)
    Short name:
    L-LDH1 PublicationUniRule annotation
    Gene namesi
    Name:ldh1 PublicationUniRule annotation
    Ordered Locus Names:TM_1867
    OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
    Taxonomic identifieri243274 [NCBI]
    Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
    Proteomesi
    • UP000008183 Componenti: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Chemistry databases

    DrugBankiDB03940 Oxamic Acid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001684061 – 319L-lactate dehydrogenaseAdd BLAST319

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei217PhosphotyrosineUniRule annotation1

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiP16115

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation2 Publications

    Protein-protein interaction databases

    STRINGi243274.TM1867

    Structurei

    Secondary structure

    1319
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details

    3D structure databases

    ProteinModelPortaliP16115
    SMRiP16115
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP16115

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni117 – 120Substrate bindingUniRule annotation4
    Regioni145 – 148Substrate bindingUniRule annotation4

    Sequence similaritiesi

    Belongs to the LDH/MDH superfamily. LDH family.UniRule annotationCurated

    Phylogenomic databases

    eggNOGiENOG4105C80 Bacteria
    COG0039 LUCA
    InParanoidiP16115
    KOiK00016
    OMAiRQPGQTR

    Family and domain databases

    Gene3Di3.90.110.10, 1 hit
    HAMAPiMF_00488 Lactate_dehydrog, 1 hit
    InterProiView protein in InterPro
    IPR001557 L-lactate/malate_DH
    IPR011304 L-lactate_DH
    IPR018177 L-lactate_DH_AS
    IPR022383 Lactate/malate_DH_C
    IPR001236 Lactate/malate_DH_N
    IPR015955 Lactate_DH/Glyco_Ohase_4_C
    IPR036291 NAD(P)-bd_dom_sf
    PfamiView protein in Pfam
    PF02866 Ldh_1_C, 1 hit
    PF00056 Ldh_1_N, 1 hit
    PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
    PRINTSiPR00086 LLDHDRGNASE
    SUPFAMiSSF51735 SSF51735, 1 hit
    SSF56327 SSF56327, 1 hit
    TIGRFAMsiTIGR01771 L-LDH-NAD, 1 hit
    PROSITEiView protein in PROSITE
    PS00064 L_LDH, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P16115-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MKIGIVGLGR VGSSTAFALL MKGFAREMVL IDVDKKRAEG DALDLIHGTP
    60 70 80 90 100
    FTRRANIYAG DYADLKGSDV VIVAAGVPQK PGETRLQLLG RNARVMKEIA
    110 120 130 140 150
    RNVSKYAPDS IVIVVTNPVD VLTYFFLKES GMDPRKVFGS GTVLDTARLR
    160 170 180 190 200
    TLIAQHCGFS PRSVHVYVIG EHGDSEVPVW SGAMIGGIPL QNMCQICQKC
    210 220 230 240 250
    DSKILENFAE KTKRAAYEII ERKGATHYAI ALAVADIVES IFFDEKRVLT
    260 270 280 290 300
    LSVYLEDYLG VKDLCISVPV TLGKHGVERI LELNLNEEEL EAFRKSASIL
    310
    KNAINEITAE ENKHQNTSG
    Length:319
    Mass (Da):34,994
    Last modified:June 1, 1994 - v2
    Checksum:iA1FB9B97CDCF290B
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti14Missing AA sequence (PubMed:2318202).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X74302 Genomic DNA Translation: CAA52355.1
    AE000512 Genomic DNA Translation: AAD36929.1
    PIRiS36863
    RefSeqiNP_229663.1, NC_000853.1
    WP_004082418.1, NZ_CP011107.1

    Genome annotation databases

    EnsemblBacteriaiAAD36929; AAD36929; TM_1867
    GeneIDi29653701
    897800
    KEGGitma:TM1867

    Similar proteinsi

    Entry informationi

    Entry nameiLDH_THEMA
    AccessioniPrimary (citable) accession number: P16115
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: June 1, 1994
    Last modified: September 12, 2018
    This is version 152 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health

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