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Protein

Fructose-bisphosphate aldolase, chloroplastic

Gene
N/A
Organism
Spinacia oleracea (Spinach)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (SOVF_097210), Glucose-6-phosphate isomerase, cytosolic (PGIC), Glucose-6-phosphate isomerase (GPIP)
  3. ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (PFP-ALPHA), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta (PFP-BETA)
  4. Fructose-bisphosphate aldolase (SOVF_190580), Fructose-bisphosphate aldolase, cytoplasmic isozyme, Fructose-bisphosphate aldolase (SOVF_128030), Fructose-bisphosphate aldolase (SOVF_094960), Fructose-bisphosphate aldolase, chloroplastic, Fructose-bisphosphate aldolase (SOVF_205230), Fructose-bisphosphate aldolase (SOVF_146670), Fructose-bisphosphate aldolase (SOVF_158110)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei93SubstrateBy similarity1
Binding sitei183SubstrateBy similarity1
Active sitei223Proton acceptorBy similarity1
Active sitei265Schiff-base intermediate with dihydroxyacetone-PBy similarity1
Sitei394Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphateBy similarity1

GO - Molecular functioni

  • fructose-bisphosphate aldolase activity Source: AgBase
  • protein self-association Source: AgBase

GO - Biological processi

  • fructose 1,6-bisphosphate metabolic process Source: AgBase
  • glycolytic process Source: UniProtKB-UniPathway

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandSchiff base

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-12899
BRENDAi4.1.2.13 5812
SABIO-RKiP16096
UniPathwayiUPA00109; UER00183

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-bisphosphate aldolase, chloroplastic (EC:4.1.2.13)
OrganismiSpinacia oleracea (Spinach)
Taxonomic identifieri3562 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesChenopodiaceaeChenopodioideaeAnserineaeSpinacia

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 46Chloroplast1 PublicationAdd BLAST46
ChainiPRO_000000111347 – 394Fructose-bisphosphate aldolase, chloroplasticAdd BLAST348

Proteomic databases

PRIDEiP16096

Interactioni

GO - Molecular functioni

  • protein self-association Source: AgBase

Structurei

3D structure databases

ProteinModelPortaliP16096
SMRiP16096
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR029768 Aldolase_I_AS
IPR013785 Aldolase_TIM
IPR000741 FBA_I
PfamiView protein in Pfam
PF00274 Glycolytic, 1 hit
PROSITEiView protein in PROSITE
PS00158 ALDOLASE_CLASS_I, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P16096-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASASLLKTS PVLDNPEFLK GQTLRIPSVA GVRFTPSGSS SLTVRASSYA
60 70 80 90 100
DELVKTAKTV ASPGRGILAM DESNATCGKR LASIGLENTE ANRQAYRTLL
110 120 130 140 150
ISAPGLGQYV SGAILFEETL YQSTTDGKKM VDVLIEQGIV PGIKVDKGWL
160 170 180 190 200
PLPGSNDESW CQGLDGLACR SAAYYQQGAR FAKWRTVVSI PNGPSALAVK
210 220 230 240 250
EAAWGLARYA AITQDNGLDP ILEPEIMLDG EHGIDRTFRV AQQVWAEVFF
260 270 280 290 300
NLAENNVLLE GSSLKPSMVG PGALSARKGP PEQVADYPLK LLHRRRGPVV
310 320 330 340 350
PGIMVLSGGQ SEVEATLNLN AMNQSPNPWH VSFSYARALQ NTCLKTWVEG
360 370 380 390
QENVKAQDFA CAKSNSLAQL GKYTGEGESE ERKKDMFVKA TLTY
Length:394
Mass (Da):42,468
Last modified:November 1, 1995 - v3
Checksum:i9D8A813E1636B274
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti260 – 263EGSS → RDP in CAA47293 (PubMed:8425060).Curated4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66814 mRNA Translation: CAA47293.1
PIRiS31090 ADSPAP

Similar proteinsi

Entry informationi

Entry nameiALFC_SPIOL
AccessioniPrimary (citable) accession number: P16096
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: November 1, 1995
Last modified: April 25, 2018
This is version 94 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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