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UniProtKB - P16083 (NQO2_HUMAN)
Protein
Ribosyldihydronicotinamide dehydrogenase [quinone]
Gene
NQO2
Organism
Homo sapiens (Human)
Status
Functioni
The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.
1 PublicationMiscellaneous
Uses dihydronicotinamide riboside (NRH) rather than NAD(P)H as an electron donor.
Catalytic activityi
- 1-(β-D-ribofuranosyl)-1,4-dihydronicotinamide + a quinone + H+ = a quinol + β-nicotinamide D-riboside1 PublicationEC:1.10.5.11 Publication
Cofactori
Protein has several cofactor binding sites:Activity regulationi
Inhibited by melatonin, resveratrol and 5-hydroxytryptamine.1 Publication
Kineticsi
- KM=28 µM for NRH2 Publications
- KM=11.6 µM for menadione2 Publications
- KM=252 µM for NADH2 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 12 | FAD2 Publications | 1 | |
Binding sitei | 156 | FAD2 Publications | 1 | |
Metal bindingi | 174 | Zinc | 1 | |
Metal bindingi | 178 | Zinc | 1 | |
Binding sitei | 194 | FAD2 Publications | 1 | |
Binding sitei | 201 | FAD2 Publications | 1 | |
Metal bindingi | 223 | Zinc | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 18 – 21 | FAD2 Publications | 4 | |
Nucleotide bindingi | 104 – 107 | FAD2 Publications | 4 | |
Nucleotide bindingi | 148 – 151 | FAD2 Publications | 4 |
GO - Molecular functioni
- chloride ion binding Source: CAFA
- dihydronicotinamide riboside quinone reductase activity Source: CAFA
- electron transfer activity Source: CAFA
- FAD binding Source: CAFA
- melatonin binding Source: CAFA
- NAD(P)H dehydrogenase (quinone) activity Source: GO_Central
- oxidoreductase activity Source: CAFA
- oxidoreductase activity, acting on other nitrogenous compounds as donors Source: CAFA
- protein homodimerization activity Source: CAFA
- resveratrol binding Source: CAFA
- zinc ion binding Source: CAFA
GO - Biological processi
- quinone catabolic process Source: CAFA
Keywordsi
Molecular function | Oxidoreductase |
Ligand | FAD, Flavoprotein, Metal-binding, Zinc |
Enzyme and pathway databases
BRENDAi | 1.10.5.1, 2681 |
PathwayCommonsi | P16083 |
Reactomei | R-HSA-211945, Phase I - Functionalization of compounds |
SABIO-RKi | P16083 |
SignaLinki | P16083 |
Names & Taxonomyi
Protein namesi | Recommended name: Ribosyldihydronicotinamide dehydrogenase [quinone] (EC:1.10.5.11 Publication)Alternative name(s): NRH dehydrogenase [quinone] 2 NRH:quinone oxidoreductase 2 Quinone reductase 2 Short name: QR2 |
Gene namesi | Name:NQO2 Synonyms:NMOR2 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:7856, NQO2 |
MIMi | 160998, gene |
neXtProti | NX_P16083 |
VEuPathDBi | HostDB:ENSG00000124588 |
Subcellular locationi
Cytoplasm and Cytosol
Cytosol
- cytosol Source: CAFA
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Nucleus
- nucleoplasm Source: HPA
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 162 | N → H: Loss of activity toward CB1954, no effect toward menadione. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 4835 |
MalaCardsi | NQO2 |
OpenTargetsi | ENSG00000124588 |
PharmGKBi | PA31745 |
Miscellaneous databases
Pharosi | P16083, Tchem |
Chemistry databases
ChEMBLi | CHEMBL3959 |
DrugBanki | DB07339, (3S)-3-hydroxy-1-methyl-2,3-dihydro-1H-indole-5,6-dione DB03541, 10-Propargyl-5,8-Dideazafolic Acid DB08228, 5,8-dimethoxy-1,4-dimethylquinolin-2(1H)-one DB08744, Casimiroin DB06695, Dabigatran etexilate DB03147, Flavin adenine dinucleotide DB01065, Melatonin DB00170, Menadione DB08190, N-[2-(2-iodo-5-methoxy-1H-indol-3-yl)ethyl]acetamide DB00157, NADH DB01087, Primaquine DB04216, Quercetin DB02709, Resveratrol DB04253, Tretazicar |
DrugCentrali | P16083 |
Genetic variation databases
BioMutai | NQO2 |
DMDMi | 317373581 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000071626 | 1 – 231 | Ribosyldihydronicotinamide dehydrogenase [quinone]Add BLAST | 231 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 80 | PhosphoserineCombined sources | 1 | |
Modified residuei | 197 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | P16083 |
jPOSTi | P16083 |
MassIVEi | P16083 |
MaxQBi | P16083 |
PaxDbi | P16083 |
PeptideAtlasi | P16083 |
PRIDEi | P16083 |
ProteomicsDBi | 53285 |
PTM databases
iPTMneti | P16083 |
PhosphoSitePlusi | P16083 |
Expressioni
Gene expression databases
Bgeei | ENSG00000124588, Expressed in nephron tubule and 235 other tissues |
ExpressionAtlasi | P16083, baseline and differential |
Genevisiblei | P16083, HS |
Organism-specific databases
HPAi | ENSG00000124588, Low tissue specificity |
Interactioni
Subunit structurei
Homodimer.
4 PublicationsBinary interactionsi
P16083
GO - Molecular functioni
- protein homodimerization activity Source: CAFA
Protein-protein interaction databases
BioGRIDi | 110898, 20 interactors |
CORUMi | P16083 |
DIPi | DIP-39704N |
IntActi | P16083, 16 interactors |
MINTi | P16083 |
STRINGi | 9606.ENSP00000369822 |
Chemistry databases
BindingDBi | P16083 |
Miscellaneous databases
RNActi | P16083, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P16083 |
SMRi | P16083 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P16083 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 127 – 129 | Substrate binding | 3 |
Sequence similaritiesi
Belongs to the NAD(P)H dehydrogenase (quinone) family.Curated
Phylogenomic databases
eggNOGi | ENOG502QWY5, Eukaryota |
GeneTreei | ENSGT00940000156563 |
HOGENOMi | CLU_058643_2_0_1 |
InParanoidi | P16083 |
OMAi | LFPIHHG |
OrthoDBi | 1394543at2759 |
PhylomeDBi | P16083 |
TreeFami | TF300296 |
Family and domain databases
Gene3Di | 3.40.50.360, 1 hit |
InterProi | View protein in InterPro IPR003680, Flavodoxin_fold IPR029039, Flavoprotein-like_sf |
Pfami | View protein in Pfam PF02525, Flavodoxin_2, 1 hit |
SUPFAMi | SSF52218, SSF52218, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
P16083-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAGKKVLIVY AHQEPKSFNG SLKNVAVDEL SRQGCTVTVS DLYAMNLEPR
60 70 80 90 100
ATDKDITGTL SNPEVFNYGV ETHEAYKQRS LASDITDEQK KVREADLVIF
110 120 130 140 150
QFPLYWFSVP AILKGWMDRV LCQGFAFDIP GFYDSGLLQG KLALLSVTTG
160 170 180 190 200
GTAEMYTKTG VNGDSRYFLW PLQHGTLHFC GFKVLAPQIS FAPEIASEEE
210 220 230
RKGMVAAWSQ RLQTIWKEEP IPCTAHWHFG Q
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ5TD07 | Q5TD07_HUMAN | Ribosyldihydronicotinamide dehydrog... | NQO2 | 193 | Annotation score: | ||
Q5TD05 | Q5TD05_HUMAN | Ribosyldihydronicotinamide dehydrog... | NQO2 | 139 | Annotation score: | ||
A2A2U4 | A2A2U4_HUMAN | Ribosyldihydronicotinamide dehydrog... | NQO2 | 63 | Annotation score: | ||
U3KQG7 | U3KQG7_HUMAN | Ribosyldihydronicotinamide dehydrog... | NQO2 | 35 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 140 | G → C in AAB60642 (PubMed:8182056).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_021399 | 16 | K → R1 PublicationCorresponds to variant dbSNP:rs28383623Ensembl. | 1 | |
Natural variantiVAR_021400 | 29 | E → G1 PublicationCorresponds to variant dbSNP:rs17136117Ensembl. | 1 | |
Natural variantiVAR_021401 | 47 | L → F7 PublicationsCorresponds to variant dbSNP:rs1143684Ensembl. | 1 | |
Natural variantiVAR_021402 | 58 | G → D1 PublicationCorresponds to variant dbSNP:rs17300141Ensembl. | 1 | |
Natural variantiVAR_021403 | 184 | V → A1 PublicationCorresponds to variant dbSNP:rs28383651Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J02888 mRNA Translation: AAA60239.1 AY299456 Genomic DNA Translation: AAB60642.3 AB050248 Genomic DNA Translation: BAB16974.1 AY855291 Genomic DNA Translation: AAW29945.1 AK311746 mRNA Translation: BAG34689.1 AL133351 Genomic DNA No translation available. CH471087 Genomic DNA Translation: EAW55107.1 BC006096 mRNA Translation: AAH06096.1 |
CCDSi | CCDS4481.1 |
PIRi | A32667 |
RefSeqi | NP_000895.2, NM_000904.4 NP_001277150.1, NM_001290221.1 NP_001305869.1, NM_001318940.1 |
Genome annotation databases
Ensembli | ENST00000338130.7; ENSP00000337773.2; ENSG00000124588.22 ENST00000380430.6; ENSP00000369795.1; ENSG00000124588.22 ENST00000380455.11; ENSP00000369822.4; ENSG00000124588.22 |
GeneIDi | 4835 |
KEGGi | hsa:4835 |
MANE-Selecti | ENST00000380455.11; ENSP00000369822.4; NM_000904.6; NP_000895.2 |
UCSCi | uc003mus.3, human |
Similar proteinsi
Cross-referencesi
Web resourcesi
NIEHS-SNPs |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J02888 mRNA Translation: AAA60239.1 AY299456 Genomic DNA Translation: AAB60642.3 AB050248 Genomic DNA Translation: BAB16974.1 AY855291 Genomic DNA Translation: AAW29945.1 AK311746 mRNA Translation: BAG34689.1 AL133351 Genomic DNA No translation available. CH471087 Genomic DNA Translation: EAW55107.1 BC006096 mRNA Translation: AAH06096.1 |
CCDSi | CCDS4481.1 |
PIRi | A32667 |
RefSeqi | NP_000895.2, NM_000904.4 NP_001277150.1, NM_001290221.1 NP_001305869.1, NM_001318940.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1QR2 | X-ray | 2.10 | A/B | 2-231 | [»] | |
1SG0 | X-ray | 1.50 | A/B | 2-231 | [»] | |
1XI2 | X-ray | 1.50 | A/B | 2-231 | [»] | |
1ZX1 | X-ray | 2.16 | A/B | 1-231 | [»] | |
2BZS | X-ray | 2.00 | A/B | 2-231 | [»] | |
2QMY | X-ray | 2.50 | A/B | 2-231 | [»] | |
2QMZ | X-ray | 2.10 | A/B | 2-231 | [»] | |
2QR2 | X-ray | 2.45 | A/B | 2-231 | [»] | |
2QWX | X-ray | 1.50 | A/B | 1-231 | [»] | |
2QX4 | X-ray | 1.65 | A/B | 2-231 | [»] | |
2QX6 | X-ray | 1.75 | A/B | 2-231 | [»] | |
2QX8 | X-ray | 1.60 | A/B | 2-231 | [»] | |
2QX9 | X-ray | 2.31 | A/B | 2-231 | [»] | |
3FW1 | X-ray | 1.75 | A | 1-231 | [»] | |
3G5M | X-ray | 1.84 | A/B | 1-231 | [»] | |
3GAM | X-ray | 1.98 | A/B | 1-231 | [»] | |
3NFR | X-ray | 1.57 | A/B | 2-231 | [»] | |
3NHF | X-ray | 2.00 | A/B | 2-231 | [»] | |
3NHJ | X-ray | 2.33 | A/B | 2-231 | [»] | |
3NHK | X-ray | 1.96 | A/B | 2-231 | [»] | |
3NHL | X-ray | 1.57 | A/B | 2-231 | [»] | |
3NHP | X-ray | 1.70 | A/B | 2-231 | [»] | |
3NHR | X-ray | 1.80 | A/B | 2-231 | [»] | |
3NHS | X-ray | 1.78 | A/B | 2-231 | [»] | |
3NHU | X-ray | 1.90 | A/B | 2-231 | [»] | |
3NHW | X-ray | 1.65 | A/B | 2-231 | [»] | |
3NHY | X-ray | 1.90 | A/B | 2-231 | [»] | |
3O2N | X-ray | 1.60 | A/B | 2-231 | [»] | |
3O73 | X-ray | 2.00 | A/B | 1-231 | [»] | |
3OVM | X-ray | 2.09 | A/B | 1-231 | [»] | |
3OWH | X-ray | 2.28 | A/B | 1-231 | [»] | |
3OWX | X-ray | 1.85 | A/B | 1-231 | [»] | |
3OX1 | X-ray | 2.00 | A/B | 1-231 | [»] | |
3OX2 | X-ray | 2.41 | A/B | 1-231 | [»] | |
3OX3 | X-ray | 1.80 | A/B | 1-231 | [»] | |
3TE7 | X-ray | 1.70 | A/B | 3-230 | [»] | |
3TEM | X-ray | 1.45 | A/B | 3-230 | [»] | |
3TZB | X-ray | 2.19 | A/B/C/D | 3-230 | [»] | |
3UXE | X-ray | 1.50 | A/B | 2-231 | [»] | |
3UXH | X-ray | 1.53 | A/B | 2-231 | [»] | |
4FGJ | X-ray | 1.35 | A/B | 1-231 | [»] | |
4FGK | X-ray | 1.40 | A/B | 1-231 | [»] | |
4FGL | X-ray | 1.20 | A/B/C/D | 1-231 | [»] | |
4GQI | X-ray | 1.95 | A/B | 2-231 | [»] | |
4GR9 | X-ray | 2.29 | A/B | 2-231 | [»] | |
4QOD | X-ray | 1.35 | A/B | 1-231 | [»] | |
4QOE | X-ray | 1.45 | A/B | 1-231 | [»] | |
4QOF | X-ray | 1.55 | A/B | 1-231 | [»] | |
4QOG | X-ray | 1.40 | A/B | 1-231 | [»] | |
4QOH | X-ray | 1.60 | A/B | 1-231 | [»] | |
4QOI | X-ray | 1.55 | A/B | 1-231 | [»] | |
4QOJ | X-ray | 1.85 | A/B | 1-231 | [»] | |
4U7F | X-ray | 1.80 | A/B | 2-231 | [»] | |
4U7G | X-ray | 1.96 | A/B | 2-231 | [»] | |
4U7H | X-ray | 1.48 | A/B | 2-231 | [»] | |
4XDG | X-ray | 1.50 | A/B | 1-231 | [»] | |
4XDH | X-ray | 1.90 | A/B | 1-231 | [»] | |
4ZVK | X-ray | 1.87 | A/B | 2-231 | [»] | |
4ZVL | X-ray | 1.90 | A/B | 2-231 | [»] | |
4ZVM | X-ray | 1.97 | A/B | 2-231 | [»] | |
4ZVN | X-ray | 1.87 | A/B | 2-231 | [»] | |
5BUC | X-ray | 1.87 | A/B | 2-231 | [»] | |
5LBT | X-ray | 1.75 | A/B | 1-231 | [»] | |
5LBU | X-ray | 1.65 | A/B | 1-231 | [»] | |
5LBW | X-ray | 1.90 | A/B | 1-231 | [»] | |
5LBY | X-ray | 1.40 | A/B | 1-231 | [»] | |
5LBZ | X-ray | 1.40 | A/B | 1-231 | [»] | |
AlphaFoldDBi | P16083 | |||||
SMRi | P16083 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 110898, 20 interactors |
CORUMi | P16083 |
DIPi | DIP-39704N |
IntActi | P16083, 16 interactors |
MINTi | P16083 |
STRINGi | 9606.ENSP00000369822 |
Chemistry databases
BindingDBi | P16083 |
ChEMBLi | CHEMBL3959 |
DrugBanki | DB07339, (3S)-3-hydroxy-1-methyl-2,3-dihydro-1H-indole-5,6-dione DB03541, 10-Propargyl-5,8-Dideazafolic Acid DB08228, 5,8-dimethoxy-1,4-dimethylquinolin-2(1H)-one DB08744, Casimiroin DB06695, Dabigatran etexilate DB03147, Flavin adenine dinucleotide DB01065, Melatonin DB00170, Menadione DB08190, N-[2-(2-iodo-5-methoxy-1H-indol-3-yl)ethyl]acetamide DB00157, NADH DB01087, Primaquine DB04216, Quercetin DB02709, Resveratrol DB04253, Tretazicar |
DrugCentrali | P16083 |
PTM databases
iPTMneti | P16083 |
PhosphoSitePlusi | P16083 |
Genetic variation databases
BioMutai | NQO2 |
DMDMi | 317373581 |
Proteomic databases
EPDi | P16083 |
jPOSTi | P16083 |
MassIVEi | P16083 |
MaxQBi | P16083 |
PaxDbi | P16083 |
PeptideAtlasi | P16083 |
PRIDEi | P16083 |
ProteomicsDBi | 53285 |
Protocols and materials databases
Antibodypediai | 9328, 535 antibodies from 34 providers |
DNASUi | 4835 |
Genome annotation databases
Ensembli | ENST00000338130.7; ENSP00000337773.2; ENSG00000124588.22 ENST00000380430.6; ENSP00000369795.1; ENSG00000124588.22 ENST00000380455.11; ENSP00000369822.4; ENSG00000124588.22 |
GeneIDi | 4835 |
KEGGi | hsa:4835 |
MANE-Selecti | ENST00000380455.11; ENSP00000369822.4; NM_000904.6; NP_000895.2 |
UCSCi | uc003mus.3, human |
Organism-specific databases
CTDi | 4835 |
DisGeNETi | 4835 |
GeneCardsi | NQO2 |
HGNCi | HGNC:7856, NQO2 |
HPAi | ENSG00000124588, Low tissue specificity |
MalaCardsi | NQO2 |
MIMi | 160998, gene |
neXtProti | NX_P16083 |
OpenTargetsi | ENSG00000124588 |
PharmGKBi | PA31745 |
VEuPathDBi | HostDB:ENSG00000124588 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QWY5, Eukaryota |
GeneTreei | ENSGT00940000156563 |
HOGENOMi | CLU_058643_2_0_1 |
InParanoidi | P16083 |
OMAi | LFPIHHG |
OrthoDBi | 1394543at2759 |
PhylomeDBi | P16083 |
TreeFami | TF300296 |
Enzyme and pathway databases
BRENDAi | 1.10.5.1, 2681 |
PathwayCommonsi | P16083 |
Reactomei | R-HSA-211945, Phase I - Functionalization of compounds |
SABIO-RKi | P16083 |
SignaLinki | P16083 |
Miscellaneous databases
BioGRID-ORCSi | 4835, 8 hits in 1081 CRISPR screens |
ChiTaRSi | NQO2, human |
EvolutionaryTracei | P16083 |
GeneWikii | NAD(P)H_dehydrogenase,_quinone_2 |
GenomeRNAii | 4835 |
Pharosi | P16083, Tchem |
PROi | PR:P16083 |
RNActi | P16083, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000124588, Expressed in nephron tubule and 235 other tissues |
ExpressionAtlasi | P16083, baseline and differential |
Genevisiblei | P16083, HS |
Family and domain databases
Gene3Di | 3.40.50.360, 1 hit |
InterProi | View protein in InterPro IPR003680, Flavodoxin_fold IPR029039, Flavoprotein-like_sf |
Pfami | View protein in Pfam PF02525, Flavodoxin_2, 1 hit |
SUPFAMi | SSF52218, SSF52218, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NQO2_HUMAN | |
Accessioni | P16083Primary (citable) accession number: P16083 Secondary accession number(s): B2R492, Q5TD04 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1990 |
Last sequence update: | January 11, 2011 | |
Last modified: | May 25, 2022 | |
This is version 221 of the entry and version 5 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 6
Human chromosome 6: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families