UniProtKB - P16047 (TGFB3_CHICK)
Transforming growth factor beta-3 proprotein
TGFB3
Functioni
Transforming growth factor beta-3 proprotein: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-3 (TGF-beta-3) chains, which constitute the regulatory and active subunit of TGF-beta-3, respectively.
By similarityRequired to maintain the Transforming growth factor beta-3 (TGF-beta-3) chain in a latent state during storage in extracellular matrix (By similarity).
Associates non-covalently with TGF-beta-3 and regulates its activation via interaction with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-3 (By similarity).
Interaction with integrins results in distortion of the Latency-associated peptide chain and subsequent release of the active TGF-beta-3 (By similarity).
By similarityTransforming growth factor beta-3: Multifunctional protein that regulates embryogenesis and cell differentiation and is required in various processes such as secondary palate development (By similarity).
Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-3 (TGF-beta-3) chains remain non-covalently linked rendering TGF-beta-3 inactive during storage in extracellular matrix (By similarity).
At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1 and LRRC32/GARP that control activation of TGF-beta-3 and maintain it in a latent state during storage in extracellular milieus (By similarity).
TGF-beta-3 is released from LAP by integrins: integrin-binding results in distortion of the LAP chain and subsequent release of the active TGF-beta-3 (By similarity).
Once activated following release of LAP, TGF-beta-3 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal (By similarity).
By similarityGO - Molecular functioni
- cytokine activity Source: GO_Central
- growth factor activity Source: UniProtKB-KW
- identical protein binding Source: AgBase
- transforming growth factor beta binding Source: AgBase
- type III transforming growth factor beta receptor binding Source: AgBase
- type II transforming growth factor beta receptor binding Source: AgBase
- type I transforming growth factor beta receptor binding Source: AgBase
GO - Biological processi
- BMP signaling pathway Source: GO_Central
- cardiac epithelial to mesenchymal transition Source: DFLAT
- cell activation Source: AgBase
- cell-cell junction organization Source: AgBase
- detection of hypoxia Source: AgBase
- embryonic digestive tract development Source: AgBase
- epithelial to mesenchymal transition involved in endocardial cushion formation Source: DFLAT
- face morphogenesis Source: AgBase
- mesenchyme migration Source: DFLAT
- negative regulation of cell population proliferation Source: AgBase
- negative regulation of macrophage cytokine production Source: AgBase
- nervous system development Source: AgBase
- ossification involved in bone remodeling Source: AgBase
- positive regulation of bone mineralization Source: AgBase
- positive regulation of cell division Source: UniProtKB-KW
- positive regulation of cell migration Source: AgBase
- positive regulation of cell population proliferation Source: AgBase
- positive regulation of collagen biosynthetic process Source: AgBase
- positive regulation of epithelial to mesenchymal transition Source: AgBase
- positive regulation of pathway-restricted SMAD protein phosphorylation Source: GO_Central
- positive regulation of transcription, DNA-templated Source: DFLAT
- regulation of cell population proliferation Source: GO_Central
- response to hypoxia Source: AgBase
- response to progesterone Source: AgBase
- salivary gland morphogenesis Source: AgBase
- SMAD protein signal transduction Source: GO_Central
- spleen development Source: AgBase
- transforming growth factor beta receptor signaling pathway Source: AgBase
Keywordsi
Molecular function | Growth factor, Mitogen |
Names & Taxonomyi
Protein namesi | Recommended name: Transforming growth factor beta-3 proproteinCleaved into the following 2 chains: |
Gene namesi | Name:TGFB3 |
Organismi | Gallus gallus (Chicken) |
Taxonomic identifieri | 9031 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archelosauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galloanserae › Galliformes › Phasianidae › Phasianinae › Gallus |
Proteomesi |
|
Subcellular locationi
Extracellular region or secreted
- extracellular matrix By similarity
Extracellular region or secreted
- Secreted By similarity
Extracellular region or secreted
- extracellular space Source: GO_Central
Other locations
- extracellular matrix Source: AgBase
Keywords - Cellular componenti
Extracellular matrix, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 23 | Sequence analysisAdd BLAST | 23 | |
ChainiPRO_0000033804 | 24 – 300 | Latency-associated peptideBy similarityAdd BLAST | 277 | |
ChainiPRO_0000033805 | 301 – 412 | Transforming growth factor beta-3By similarityAdd BLAST | 112 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 74 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 135 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 142 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 307 ↔ 316 | By similarity | ||
Disulfide bondi | 315 ↔ 378 | By similarity | ||
Disulfide bondi | 344 ↔ 409 | By similarity | ||
Disulfide bondi | 348 ↔ 411 | By similarity | ||
Disulfide bondi | 377 | InterchainBy similarity |
Post-translational modificationi
Keywords - PTMi
Cleavage on pair of basic residues, Disulfide bond, GlycoproteinProteomic databases
PaxDbi | P16047 |
Interactioni
Subunit structurei
Latency-associated peptide: Homodimer; disulfide-linked. Latency-associated peptide:
Interacts with Transforming growth factor beta-3 (TGF-beta-3) chain; interaction is non-covalent and maintains (TGF-beta-3) in a latent state (By similarity). Latency-associated peptide:
Interacts (via cell attachment site) with integrins, leading to release of the active TGF-beta-3 (By similarity). Transforming growth factor beta-3: Homodimer; disulfide-linked (By similarity). Transforming growth factor beta-3:
Interacts with TGF-beta receptors (TGFBR1 and TGFBR2), leading to signal transduction (By similarity).
By similarityGO - Molecular functioni
- cytokine activity Source: GO_Central
- growth factor activity Source: UniProtKB-KW
- identical protein binding Source: AgBase
- transforming growth factor beta binding Source: AgBase
- type III transforming growth factor beta receptor binding Source: AgBase
- type II transforming growth factor beta receptor binding Source: AgBase
- type I transforming growth factor beta receptor binding Source: AgBase
Protein-protein interaction databases
STRINGi | 9031.ENSGALP00000036912 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 261 – 263 | Cell attachment siteBy similarity | 3 |
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG3900, Eukaryota |
InParanoidi | P16047 |
OrthoDBi | 643840at2759 |
PhylomeDBi | P16047 |
Family and domain databases
Gene3Di | 2.10.90.10, 1 hit |
InterProi | View protein in InterPro IPR029034, Cystine-knot_cytokine IPR001839, TGF-b_C IPR001111, TGF-b_propeptide IPR016319, TGF-beta IPR015615, TGF-beta-rel IPR015618, TGFB3 IPR017948, TGFb_CS |
PANTHERi | PTHR11848, PTHR11848, 1 hit |
Pfami | View protein in Pfam PF00019, TGF_beta, 1 hit PF00688, TGFb_propeptide, 1 hit |
PIRSFi | PIRSF001787, TGF-beta, 1 hit |
PRINTSi | PR01423, TGFBETA PR01426, TGFBETA3 |
SMARTi | View protein in SMART SM00204, TGFB, 1 hit |
SUPFAMi | SSF57501, SSF57501, 1 hit |
PROSITEi | View protein in PROSITE PS00250, TGF_BETA_1, 1 hit PS51362, TGF_BETA_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MKMYAQRALV LLSLLSFATV SLALSSCTTL DLEHIKKKRV EAIRGQILSK
60 70 80 90 100
LRLTSPPESV GPAHVPYQIL ALYNSTRELL EEMEEEKEES CSQENTESEY
110 120 130 140 150
YAKEIHKFDM IQGLPEHNEL GICPKGVTSN VFRFNVSSAE KNSTNLFRAE
160 170 180 190 200
FRVLRVPNPS SKRSEQRIEL FQILRPDEHI AKQRYLSGRN VQTRGSPEWL
210 220 230 240 250
SFDVTDTVRE WLLHRESNLG LEISIHCPCH TFQPNGDILE NLHEVLEIKF
260 270 280 290 300
KGIDSEDDYG RGDLGRLKKQ KDLHNPHLIL MMLPPHRLES PTLGGQRKKR
310 320 330 340 350
ALDTNYCFRN LEENCCVRPL YIELPTDLGW KWVHEPKGYF ANFCSGPCPY
360 370 380 390 400
LRSADTTHST VLGLYNTLNP EASASPCCVP QDLEPLTILY YVGRTPKVEQ
410
LSNMVVKSCK CS
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 323 – 326 | ELPT → DFRQ in AAA49089 (PubMed:3211158).Curated | 4 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M31154 mRNA Translation: AAA49089.1 X58127 , X60055, X60091, X60090 Genomic DNA Translation: CAA41128.2 S46000 Genomic DNA Translation: AAB23575.1 |
PIRi | A34939 |
RefSeqi | NP_990785.1, NM_205454.1 |
Genome annotation databases
GeneIDi | 396438 |
KEGGi | gga:396438 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M31154 mRNA Translation: AAA49089.1 X58127 , X60055, X60091, X60090 Genomic DNA Translation: CAA41128.2 S46000 Genomic DNA Translation: AAB23575.1 |
PIRi | A34939 |
RefSeqi | NP_990785.1, NM_205454.1 |
3D structure databases
AlphaFoldDBi | P16047 |
SMRi | P16047 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9031.ENSGALP00000036912 |
Proteomic databases
PaxDbi | P16047 |
Genome annotation databases
GeneIDi | 396438 |
KEGGi | gga:396438 |
Organism-specific databases
CTDi | 7043 |
Phylogenomic databases
eggNOGi | KOG3900, Eukaryota |
InParanoidi | P16047 |
OrthoDBi | 643840at2759 |
PhylomeDBi | P16047 |
Miscellaneous databases
PROi | PR:P16047 |
Family and domain databases
Gene3Di | 2.10.90.10, 1 hit |
InterProi | View protein in InterPro IPR029034, Cystine-knot_cytokine IPR001839, TGF-b_C IPR001111, TGF-b_propeptide IPR016319, TGF-beta IPR015615, TGF-beta-rel IPR015618, TGFB3 IPR017948, TGFb_CS |
PANTHERi | PTHR11848, PTHR11848, 1 hit |
Pfami | View protein in Pfam PF00019, TGF_beta, 1 hit PF00688, TGFb_propeptide, 1 hit |
PIRSFi | PIRSF001787, TGF-beta, 1 hit |
PRINTSi | PR01423, TGFBETA PR01426, TGFBETA3 |
SMARTi | View protein in SMART SM00204, TGFB, 1 hit |
SUPFAMi | SSF57501, SSF57501, 1 hit |
PROSITEi | View protein in PROSITE PS00250, TGF_BETA_1, 1 hit PS51362, TGF_BETA_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TGFB3_CHICK | |
Accessioni | P16047Primary (citable) accession number: P16047 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1990 |
Last sequence update: | August 29, 2001 | |
Last modified: | May 25, 2022 | |
This is version 139 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families