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UniProtKB - P16027 (DHM1_METEA)
Protein
Methanol dehydrogenase [cytochrome c] subunit 1
Gene
moxF
Organism
Methylorubrum extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1) (Methylobacterium extorquens)
Status
Functioni
Catalyzes the oxidation of primary alcohols including methanol.
Catalytic activityi
- a primary alcohol + 2 Fe(III)-[cytochrome cL] = an aldehyde + 2 Fe(II)-[cytochrome cL] + 2 H+1 PublicationEC:1.1.2.71 Publication
Cofactori
Protein has several cofactor binding sites:- pyrroloquinoline quinoneNote: Binds 1 PQQ group per subunit. PQQ is inserted between disulfide Cys-130-Cys-131 and the indole ring of Trp-270.
- Ca2+Note: Binds 1 Ca2+ ion per subunit.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 204 | Calcium | 1 | |
Metal bindingi | 288 | Calcium | 1 | |
Active sitei | 330 | Proton acceptorBy similarity | 1 |
GO - Molecular functioni
- alcohol dehydrogenase (cytochrome c(L)) activity Source: UniProtKB-EC
- calcium ion binding Source: InterPro
GO - Biological processi
- methanol metabolic process Source: UniProtKB-KW
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Methanol utilization |
Ligand | Calcium, Metal-binding, PQQ |
Enzyme and pathway databases
BRENDAi | 1.1.2.7, 3296 |
Names & Taxonomyi
Protein namesi | Recommended name: Methanol dehydrogenase [cytochrome c] subunit 1 (EC:1.1.2.7)Alternative name(s): MDH large subunit alpha MEDH |
Gene namesi | Name:moxF Synonyms:mxaF Ordered Locus Names:MexAM1_META1p4538 |
Organismi | Methylorubrum extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1) (Methylobacterium extorquens) |
Taxonomic identifieri | 272630 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Alphaproteobacteria › Hyphomicrobiales › Methylobacteriaceae › Methylorubrum › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
Note: Periplasmic, but associated with inner membrane.
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- outer membrane-bounded periplasmic space Source: InterPro
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 130 | C → S: Inactive. 1 Publication | 1 | |
Mutagenesisi | 131 | C → S: Inactive. 1 Publication | 1 | |
Mutagenesisi | 330 | D → E: Lower affinity for methanol. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 27 | 1 PublicationAdd BLAST | 27 | |
ChainiPRO_0000025566 | 28 – 626 | Methanol dehydrogenase [cytochrome c] subunit 1Add BLAST | 599 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 130 ↔ 131 | 1 Publication | ||
Disulfide bondi | 413 ↔ 442 | 1 Publication |
Keywords - PTMi
Disulfide bondInteractioni
Subunit structurei
Heterotetramer composed of 2 alpha and 2 beta subunits.
1 PublicationProtein-protein interaction databases
STRINGi | 272630.MexAM1_META1p4538 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P16027 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P16027 |
Family & Domainsi
Sequence similaritiesi
Belongs to the bacterial PQQ dehydrogenase family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG4993, Bacteria |
HOGENOMi | CLU_018478_0_0_5 |
OrthoDBi | 1377603at2 |
Family and domain databases
InterProi | View protein in InterPro IPR018391, PQQ_beta_propeller_repeat IPR017512, PQQ_MeOH/EtOH_DH IPR002372, PQQ_repeat IPR011047, Quinoprotein_ADH-like_supfam IPR001479, Quinoprotein_DH_CS |
Pfami | View protein in Pfam PF01011, PQQ, 1 hit PF13360, PQQ_2, 1 hit |
SMARTi | View protein in SMART SM00564, PQQ, 3 hits |
SUPFAMi | SSF50998, SSF50998, 1 hit |
TIGRFAMsi | TIGR03075, PQQ_enz_alc_DH, 1 hit |
PROSITEi | View protein in PROSITE PS00363, BACTERIAL_PQQ_1, 1 hit PS00364, BACTERIAL_PQQ_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P16027-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSRFVTSVSA LAMLALAPAA LSSGAYANDK LVELSKSDDN WVMPGKNYDS
60 70 80 90 100
NNFSDLKQIN KGNVKQLRPA WTFSTGLLNG HEGAPLVVDG KMYIHTSFPN
110 120 130 140 150
NTFALGLDDP GTILWQDKPK QNPAARAVAC CDLVNRGLAY WPGDGKTPAL
160 170 180 190 200
ILKTQLDGNV AALNAETGET VWKVENSDIK VGSTLTIAPY VVKDKVIIGS
210 220 230 240 250
SGAELGVRGY LTAYDVKTGE QVWRAYATGP DKDLLLASDF NIKNPHYGQK
260 270 280 290 300
GLGTGTWEGD AWKIGGGTNW GWYAYDPGTN LIYFGTGNPA PWNETMRPGD
310 320 330 340 350
NKWTMTIFGR DADTGEAKFG YQKTPHDEWD YAGVNVMMLS EQKDKDGKAR
360 370 380 390 400
KLLTHPDRNG IVYTLDRTDG ALVSANKLDD TVNVFKSVDL KTGQPVRDPE
410 420 430 440 450
YGTRMDHLAK DICPSAMGYH NQGHDSYDPK RELFFMGINH ICMDWEPFML
460 470 480 490 500
PYRAGQFFVG ATLNMYPGPK GDRQNYEGLG QIKAYNAITG DYKWEKMERF
510 520 530 540 550
AVWGGTMATA GDLVFYGTLD GYLKARDSDT GDLLWKFKIP SGAIGYPMTY
560 570 580 590 600
THKGTQYVAI YYGVGGWPGV GLVFDLADPT AGLGAVGAFK KLANYTQMGG
610 620
GVVVFSLDGK GPYDDPNVGE WKSAAK
Sequence cautioni
The sequence ACS42169 differs from that shown. Reason: Erroneous initiation.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M31108 Genomic DNA Translation: AAA25380.1 CP001510 Genomic DNA Translation: ACS42169.1 Different initiation. |
PIRi | JQ0706 |
RefSeqi | WP_003599114.1, NC_012988.1 |
Genome annotation databases
EnsemblBacteriai | ACS42169; ACS42169; MexAM1_META1p4538 |
KEGGi | mea:Mex_1p4538 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M31108 Genomic DNA Translation: AAA25380.1 CP001510 Genomic DNA Translation: ACS42169.1 Different initiation. |
PIRi | JQ0706 |
RefSeqi | WP_003599114.1, NC_012988.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1H4I | X-ray | 1.94 | A/C | 28-626 | [»] | |
1H4J | X-ray | 3.00 | A/C/E/G | 28-626 | [»] | |
1W6S | X-ray | 1.20 | A/C | 28-626 | [»] | |
SMRi | P16027 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 272630.MexAM1_META1p4538 |
Genome annotation databases
EnsemblBacteriai | ACS42169; ACS42169; MexAM1_META1p4538 |
KEGGi | mea:Mex_1p4538 |
Phylogenomic databases
eggNOGi | COG4993, Bacteria |
HOGENOMi | CLU_018478_0_0_5 |
OrthoDBi | 1377603at2 |
Enzyme and pathway databases
BRENDAi | 1.1.2.7, 3296 |
Miscellaneous databases
EvolutionaryTracei | P16027 |
Family and domain databases
InterProi | View protein in InterPro IPR018391, PQQ_beta_propeller_repeat IPR017512, PQQ_MeOH/EtOH_DH IPR002372, PQQ_repeat IPR011047, Quinoprotein_ADH-like_supfam IPR001479, Quinoprotein_DH_CS |
Pfami | View protein in Pfam PF01011, PQQ, 1 hit PF13360, PQQ_2, 1 hit |
SMARTi | View protein in SMART SM00564, PQQ, 3 hits |
SUPFAMi | SSF50998, SSF50998, 1 hit |
TIGRFAMsi | TIGR03075, PQQ_enz_alc_DH, 1 hit |
PROSITEi | View protein in PROSITE PS00363, BACTERIAL_PQQ_1, 1 hit PS00364, BACTERIAL_PQQ_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DHM1_METEA | |
Accessioni | P16027Primary (citable) accession number: P16027 Secondary accession number(s): C5AQA9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1990 |
Last sequence update: | April 1, 1990 | |
Last modified: | February 23, 2022 | |
This is version 163 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencingDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families