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Protein

Beta-galactoside alpha-2,6-sialyltransferase 1

Gene

ST6GAL1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transfers sialic acid from CMP-sialic acid to galactose-containing acceptor substrates.2 Publications

Catalytic activityi

CMP-N-acetyl-beta-neuraminate + beta-D-galactosyl-R = CMP + N-acetyl-alpha-neuraminyl-(2->6)-beta-D-galactosyl-R.2 Publications

Activity regulationi

Inhibited by CTP.1 Publication

Kineticsi

  1. KM=530 µM for CMP-NeuAc1 Publication
  1. Vmax=1.074 pmol/min/µg enzyme1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei189Substrate; via amide nitrogenCombined sources1 Publication1
Binding sitei212Substrate1 Publication1
Binding sitei233Substrate1 Publication1
Binding sitei353Substrate; via carbonyl oxygenCombined sources1 Publication1
Binding sitei354SubstrateCombined sources1 Publication1
Binding sitei365SubstrateCombined sources1 Publication1
Binding sitei369SubstrateCurated1
Binding sitei370Substrate1 Publication1
Binding sitei376SubstrateCombined sources1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:HS01118-MONOMER
BRENDAi2.4.99.1 2681
ReactomeiR-HSA-4085001 Sialic acid metabolism
R-HSA-975577 N-Glycan antennae elongation
R-HSA-977068 Termination of O-glycan biosynthesis
UniPathwayi
UPA00378

Protein family/group databases

CAZyiGT29 Glycosyltransferase Family 29

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactoside alpha-2,6-sialyltransferase 1 (EC:2.4.99.12 Publications)
Short name:
Alpha 2,6-ST 1
Alternative name(s):
B-cell antigen CD75
CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1
ST6Gal I
Short name:
ST6GalI
Sialyltransferase 1
Gene namesi
Name:ST6GAL1
Synonyms:SIAT1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiHostDB:ENSG00000073849.14
HGNCiHGNC:10860 ST6GAL1
MIMi109675 gene
neXtProtiNX_P15907

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 9Cytoplasmic9
Transmembranei10 – 26Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST17
Topological domaini27 – 406LumenalSequence analysisAdd BLAST380

Keywords - Cellular componenti

Golgi apparatus, Membrane, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi24C → G: No effect on dimerization and on location at the Golgi stack. 1 Publication1

Organism-specific databases

DisGeNETi6480
OpenTargetsiENSG00000073849
PharmGKBiPA35762

Chemistry databases

ChEMBLiCHEMBL3596075

Polymorphism and mutation databases

BioMutaiST6GAL1
DMDMi115445

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001492491 – 406Beta-galactoside alpha-2,6-sialyltransferase 1Add BLAST406

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi142 ↔ 406Combined sources1 Publication
Glycosylationi149N-linked (GlcNAc...) asparagineCombined sources2 Publications1
Glycosylationi161N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi184 ↔ 335Combined sources1 Publication
Disulfide bondi353 ↔ 364Combined sources1 Publication
Modified residuei369PhosphotyrosineCombined sources1

Post-translational modificationi

The soluble form derives from the membrane form by proteolytic processing.By similarity
The HB-6, CDW75, and CD76 differentiation antigens are cell-surface carbohydrate determinants generated by this enzyme.
N-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiP15907
MaxQBiP15907
PaxDbiP15907
PeptideAtlasiP15907
PRIDEiP15907
ProteomicsDBi53240

PTM databases

iPTMnetiP15907
PhosphoSitePlusiP15907

Expressioni

Gene expression databases

BgeeiENSG00000073849 Expressed in 236 organ(s), highest expression level in liver
CleanExiHS_ST6GAL1
ExpressionAtlasiP15907 baseline and differential
GenevisibleiP15907 HS

Organism-specific databases

HPAiCAB015018
CAB016122

Interactioni

Subunit structurei

Monomer and homodimer.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi112374, 20 interactors
IntActiP15907, 2 interactors
STRINGi9606.ENSP00000169298

Chemistry databases

BindingDBiP15907

Structurei

Secondary structure

1406
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP15907
SMRiP15907
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni322 – 324Substrate bindingCombined sources1 Publication3

Sequence similaritiesi

Belongs to the glycosyltransferase 29 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2692 Eukaryota
ENOG410XT8P LUCA
GeneTreeiENSGT00550000074444
HOGENOMiHOG000013206
HOVERGENiHBG052853
InParanoidiP15907
KOiK00778
OMAiICVWKER
OrthoDBiEOG091G07C7
PhylomeDBiP15907
TreeFamiTF323961

Family and domain databases

Gene3Di3.90.1480.20, 1 hit
InterProiView protein in InterPro
IPR001675 Glyco_trans_29
IPR038578 GT29-like_sf
IPR012163 Sialyl_trans
PfamiView protein in Pfam
PF00777 Glyco_transf_29, 1 hit
PIRSFiPIRSF005557 Sialyl_trans, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 13 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P15907-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MIHTNLKKKF SCCVLVFLLF AVICVWKEKK KGSYYDSFKL QTKEFQVLKS
60 70 80 90 100
LGKLAMGSDS QSVSSSSTQD PHRGRQTLGS LRGLAKAKPE ASFQVWNKDS
110 120 130 140 150
SSKNLIPRLQ KIWKNYLSMN KYKVSYKGPG PGIKFSAEAL RCHLRDHVNV
160 170 180 190 200
SMVEVTDFPF NTSEWEGYLP KESIRTKAGP WGRCAVVSSA GSLKSSQLGR
210 220 230 240 250
EIDDHDAVLR FNGAPTANFQ QDVGTKTTIR LMNSQLVTTE KRFLKDSLYN
260 270 280 290 300
EGILIVWDPS VYHSDIPKWY QNPDYNFFNN YKTYRKLHPN QPFYILKPQM
310 320 330 340 350
PWELWDILQE ISPEEIQPNP PSSGMLGIII MMTLCDQVDI YEFLPSKRKT
360 370 380 390 400
DVCYYYQKFF DSACTMGAYH PLLYEKNLVK HLNQGTDEDI YLLGKATLPG

FRTIHC
Length:406
Mass (Da):46,605
Last modified:April 1, 1990 - v1
Checksum:iAC1E24A3875CF00F
GO
Isoform 2 (identifier: P15907-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-231: Missing.

Note: No experimental confirmation available.
Show »
Length:175
Mass (Da):20,764
Checksum:i2A31915E3D582F4B
GO

Computationally mapped potential isoform sequencesi

There are 13 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H7C472H7C472_HUMAN
Beta-galactoside alpha-2,6-sialyltr...
ST6GAL1
178Annotation score:
C9JH16C9JH16_HUMAN
Beta-galactoside alpha-2,6-sialyltr...
ST6GAL1
115Annotation score:
C9J6X5C9J6X5_HUMAN
Beta-galactoside alpha-2,6-sialyltr...
ST6GAL1
127Annotation score:
C9JVK7C9JVK7_HUMAN
Beta-galactoside alpha-2,6-sialyltr...
ST6GAL1
52Annotation score:
C9K0R8C9K0R8_HUMAN
Beta-galactoside alpha-2,6-sialyltr...
ST6GAL1
113Annotation score:
C9JI90C9JI90_HUMAN
Beta-galactoside alpha-2,6-sialyltr...
ST6GAL1
97Annotation score:
C9JFM4C9JFM4_HUMAN
Beta-galactoside alpha-2,6-sialyltr...
ST6GAL1
98Annotation score:
C9JR47C9JR47_HUMAN
Beta-galactoside alpha-2,6-sialyltr...
ST6GAL1
91Annotation score:
C9JPG6C9JPG6_HUMAN
Beta-galactoside alpha-2,6-sialyltr...
ST6GAL1
45Annotation score:
C9JTR0C9JTR0_HUMAN
Beta-galactoside alpha-2,6-sialyltr...
ST6GAL1
42Annotation score:
There are more potential isoformsShow all

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti27K → L in CAA38246 (PubMed:2373995).Curated1
Sequence conflicti72 – 73HR → T in CAA38246 (PubMed:2373995).Curated2
Sequence conflicti144L → P in CAA38246 (PubMed:2373995).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0560761 – 231Missing in isoform 2. 1 PublicationAdd BLAST231

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17247 mRNA Translation: CAA35111.1
X54363 mRNA Translation: CAA38246.1
X62822 mRNA Translation: CAA44634.1
AK292879 mRNA Translation: BAF85568.1
AK312023 mRNA Translation: BAG34960.1
AC007488 Genomic DNA No translation available.
AC007690 Genomic DNA No translation available.
CH471052 Genomic DNA Translation: EAW78160.1
CH471052 Genomic DNA Translation: EAW78161.1
CH471052 Genomic DNA Translation: EAW78162.1
CH471052 Genomic DNA Translation: EAW78164.1
BC031476 mRNA Translation: AAH31476.1
BC040009 mRNA Translation: AAH40009.1
CCDSiCCDS3285.1 [P15907-1]
CCDS46973.1 [P15907-2]
PIRiA41734
RefSeqiNP_003023.1, NM_003032.2 [P15907-1]
NP_775323.1, NM_173216.2 [P15907-1]
NP_775324.1, NM_173217.2 [P15907-2]
XP_005247776.1, XM_005247719.1
XP_005247777.1, XM_005247720.1
XP_006713797.1, XM_006713734.1
XP_011511387.1, XM_011513085.1
XP_011511388.1, XM_011513086.1
XP_016862554.1, XM_017007065.1
XP_016862555.1, XM_017007066.1
XP_016862556.1, XM_017007067.1
XP_016862557.1, XM_017007068.1
UniGeneiHs.207459

Genome annotation databases

EnsembliENST00000169298; ENSP00000169298; ENSG00000073849 [P15907-1]
ENST00000448044; ENSP00000389337; ENSG00000073849 [P15907-1]
ENST00000457772; ENSP00000412221; ENSG00000073849 [P15907-2]
GeneIDi6480
KEGGihsa:6480
UCSCiuc003frb.4 human [P15907-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

ST6Gal I

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17247 mRNA Translation: CAA35111.1
X54363 mRNA Translation: CAA38246.1
X62822 mRNA Translation: CAA44634.1
AK292879 mRNA Translation: BAF85568.1
AK312023 mRNA Translation: BAG34960.1
AC007488 Genomic DNA No translation available.
AC007690 Genomic DNA No translation available.
CH471052 Genomic DNA Translation: EAW78160.1
CH471052 Genomic DNA Translation: EAW78161.1
CH471052 Genomic DNA Translation: EAW78162.1
CH471052 Genomic DNA Translation: EAW78164.1
BC031476 mRNA Translation: AAH31476.1
BC040009 mRNA Translation: AAH40009.1
CCDSiCCDS3285.1 [P15907-1]
CCDS46973.1 [P15907-2]
PIRiA41734
RefSeqiNP_003023.1, NM_003032.2 [P15907-1]
NP_775323.1, NM_173216.2 [P15907-1]
NP_775324.1, NM_173217.2 [P15907-2]
XP_005247776.1, XM_005247719.1
XP_005247777.1, XM_005247720.1
XP_006713797.1, XM_006713734.1
XP_011511387.1, XM_011513085.1
XP_011511388.1, XM_011513086.1
XP_016862554.1, XM_017007065.1
XP_016862555.1, XM_017007066.1
XP_016862556.1, XM_017007067.1
XP_016862557.1, XM_017007068.1
UniGeneiHs.207459

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4JS1X-ray2.09A89-406[»]
4JS2X-ray2.30A89-406[»]
ProteinModelPortaliP15907
SMRiP15907
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112374, 20 interactors
IntActiP15907, 2 interactors
STRINGi9606.ENSP00000169298

Chemistry databases

BindingDBiP15907
ChEMBLiCHEMBL3596075

Protein family/group databases

CAZyiGT29 Glycosyltransferase Family 29

PTM databases

iPTMnetiP15907
PhosphoSitePlusiP15907

Polymorphism and mutation databases

BioMutaiST6GAL1
DMDMi115445

Proteomic databases

EPDiP15907
MaxQBiP15907
PaxDbiP15907
PeptideAtlasiP15907
PRIDEiP15907
ProteomicsDBi53240

Protocols and materials databases

DNASUi6480
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000169298; ENSP00000169298; ENSG00000073849 [P15907-1]
ENST00000448044; ENSP00000389337; ENSG00000073849 [P15907-1]
ENST00000457772; ENSP00000412221; ENSG00000073849 [P15907-2]
GeneIDi6480
KEGGihsa:6480
UCSCiuc003frb.4 human [P15907-1]

Organism-specific databases

CTDi6480
DisGeNETi6480
EuPathDBiHostDB:ENSG00000073849.14
GeneCardsiST6GAL1
HGNCiHGNC:10860 ST6GAL1
HPAiCAB015018
CAB016122
MIMi109675 gene
neXtProtiNX_P15907
OpenTargetsiENSG00000073849
PharmGKBiPA35762
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2692 Eukaryota
ENOG410XT8P LUCA
GeneTreeiENSGT00550000074444
HOGENOMiHOG000013206
HOVERGENiHBG052853
InParanoidiP15907
KOiK00778
OMAiICVWKER
OrthoDBiEOG091G07C7
PhylomeDBiP15907
TreeFamiTF323961

Enzyme and pathway databases

UniPathwayi
UPA00378

BioCyciMetaCyc:HS01118-MONOMER
BRENDAi2.4.99.1 2681
ReactomeiR-HSA-4085001 Sialic acid metabolism
R-HSA-975577 N-Glycan antennae elongation
R-HSA-977068 Termination of O-glycan biosynthesis

Miscellaneous databases

ChiTaRSiST6GAL1 human
GeneWikiiST6GAL1
GenomeRNAii6480
PROiPR:P15907
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000073849 Expressed in 236 organ(s), highest expression level in liver
CleanExiHS_ST6GAL1
ExpressionAtlasiP15907 baseline and differential
GenevisibleiP15907 HS

Family and domain databases

Gene3Di3.90.1480.20, 1 hit
InterProiView protein in InterPro
IPR001675 Glyco_trans_29
IPR038578 GT29-like_sf
IPR012163 Sialyl_trans
PfamiView protein in Pfam
PF00777 Glyco_transf_29, 1 hit
PIRSFiPIRSF005557 Sialyl_trans, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSIAT1_HUMAN
AccessioniPrimary (citable) accession number: P15907
Secondary accession number(s): A8KA14, B2R513, D3DNV3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: November 7, 2018
This is version 192 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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