UniProtKB - P15891 (ABP1_YEAST)
Protein
Actin-binding protein
Gene
ABP1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Regulates ARP2/3 complex-mediated actin assembly. Recruits ARP2/3 complex to sides of preexisting actin filaments, which may promote nucleation or stabilization of filament branches. Binds to actin filaments, but not actin monomers. Actin binding is required for ARP2/3 complex activation. May also have a role in linking the actin cytoskeleton to endocytosis. recruits components of the endocytotic machinery to cortical actin patches, known sites of endocytosis.1 Publication
Miscellaneous
Present with 606 molecules/cell in log phase SD medium.1 Publication
GO - Molecular functioni
- actin filament binding Source: SGD
GO - Biological processi
- actin cortical patch assembly Source: SGD
- barbed-end actin filament capping Source: SGD
- positive regulation of Arp2/3 complex-mediated actin nucleation Source: SGD
- protein localization to actin cortical patch Source: SGD
- regulation of actin filament polymerization Source: GO_Central
Keywordsi
Molecular function | Actin-binding |
Enzyme and pathway databases
BioCyci | YEAST:G3O-29382-MONOMER |
Reactomei | R-SCE-6798695 Neutrophil degranulation R-SCE-8856828 Clathrin-mediated endocytosis |
Names & Taxonomyi
Protein namesi | Recommended name: Actin-binding protein |
Gene namesi | Name:ABP1 Ordered Locus Names:YCR088W ORF Names:YCR88W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YCR088W |
SGDi | S000000684 ABP1 |
Subcellular locationi
Cytoskeleton
- actin patch 3 Publications
Note: Cortical actin patches.
Cytoskeleton
- actin cortical patch Source: SGD
- cortical actin cytoskeleton Source: GO_Central
Plasma Membrane
- plasma membrane Source: GO_Central
Other locations
- cell cortex Source: SGD
- cytoplasm Source: SGD
- mating projection tip Source: SGD
- site of polarized growth Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, CytoskeletonPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 21 | K → A in ABP1-1; moderately reduces actin binding and partially reduces ARP2/3 complex activation; when associated with A-24. 1 Publication | 1 | |
Mutagenesisi | 24 | R → A in ABP1-1; moderately reduces actin binding and partially reduces ARP2/3 complex activation; when associated with A-21. 1 Publication | 1 | |
Mutagenesisi | 80 | K → A in ABP1-2; strongly reduces actin binding and abolishes ARP2/3 complex activation. 1 Publication | 1 | |
Mutagenesisi | 94 | K → A in ABP1-3; reduces actin binding and abolishes ARP2/3 complex activation; when associated with A-96. 1 Publication | 1 | |
Mutagenesisi | 96 | R → A in ABP1-3; reduces actin binding and abolishes ARP2/3 complex activation; when associated with A-94. 1 Publication | 1 | |
Mutagenesisi | 122 | D → A in ABP1-4; no effect; when associated with A-125. 1 Publication | 1 | |
Mutagenesisi | 125 | D → A in ABP1-4; no effect; when associated with A-122. 1 Publication | 1 | |
Mutagenesisi | 134 | K → A in ABP1-5; moderately reduces actin binding and abolishes ARP2/3 complex activation. 1 Publication | 1 | |
Mutagenesisi | 201 – 203 | DDW → AAA: Abolishes ARP2/3 complex activation, but not actin binding. 1 Publication | 3 | |
Mutagenesisi | 437 – 439 | DDW → AAA: Abolishes ARP2/3 complex activation, but not actin binding. 1 Publication | 3 | |
Mutagenesisi | 569 | W → A: Abolishes protein binding. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources1 Publication | |||
ChainiPRO_0000064429 | 2 – 592 | Actin-binding proteinAdd BLAST | 591 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources1 Publication | 1 | |
Modified residuei | 165 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 167 | PhosphoserineCombined sources | 1 | |
Modified residuei | 169 | PhosphoserineCombined sources | 1 | |
Modified residuei | 181 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 183 | PhosphoserineCombined sources | 1 | |
Modified residuei | 313 | PhosphoserineCombined sources | 1 | |
Modified residuei | 365 | PhosphoserineCombined sources | 1 | |
Modified residuei | 389 | PhosphoserineCombined sources | 1 | |
Modified residuei | 458 | PhosphoserineCombined sources | 1 | |
Cross-linki | 464 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources | ||
Modified residuei | 481 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
The actin depolymerizing factor homology (ADF) domain mediates actin filament binding.
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
MaxQBi | P15891 |
PaxDbi | P15891 |
PRIDEi | P15891 |
PTM databases
iPTMneti | P15891 |
Interactioni
Subunit structurei
Binds F-actin, but not G-actin.
Interacts with the ARP2/3 complex.
Interacts with APP1, ARK1, PRK1, SCP1, SRV2 and YIR003W via its SH3 domain.
Interacts with the SH3 domain of RVS167 and with SLA1.
6 PublicationsBinary interactionsi
GO - Molecular functioni
- actin filament binding Source: SGD
Protein-protein interaction databases
BioGridi | 31060, 163 interactors |
DIPi | DIP-534N |
IntActi | P15891, 50 interactors |
MINTi | P15891 |
STRINGi | 4932.YCR088W |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P15891 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P15891 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 7 – 136 | ADF-HPROSITE-ProRule annotationAdd BLAST | 130 | |
Repeati | 200 – 209 | 1 | 10 | |
Repeati | 436 – 445 | 2 | 10 | |
Domaini | 532 – 592 | SH3PROSITE-ProRule annotationAdd BLAST | 61 | |
Repeati | 566 – 575 | 3 | 10 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 200 – 575 | 3 X 10 AA approximate repeats (acidic)Add BLAST | 376 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 518 – 522 | Poly-Pro | 5 |
Sequence similaritiesi
Belongs to the ABP1 family.Curated
Keywords - Domaini
Repeat, SH3 domainPhylogenomic databases
HOGENOMi | HOG000246671 |
InParanoidi | P15891 |
KOi | K20520 |
OMAi | YKPTKPH |
Family and domain databases
CDDi | cd11961 SH3_Abp1_fungi_C2, 1 hit |
Gene3Di | 3.40.20.10, 1 hit |
InterProi | View protein in InterPro IPR035718 Abp1_fungi_SH3_C2 IPR002108 ADF-H IPR029006 ADF-H/Gelsolin-like_dom_sf IPR036028 SH3-like_dom_sf IPR001452 SH3_domain |
Pfami | View protein in Pfam PF00241 Cofilin_ADF, 1 hit PF00018 SH3_1, 1 hit |
PRINTSi | PR00452 SH3DOMAIN |
SMARTi | View protein in SMART SM00102 ADF, 1 hit SM00326 SH3, 1 hit |
SUPFAMi | SSF50044 SSF50044, 1 hit |
PROSITEi | View protein in PROSITE PS51263 ADF_H, 1 hit PS50002 SH3, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P15891-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MALEPIDYTT HSREIDAEYL KIVRGSDPDT TWLIISPNAK KEYEPESTGS
60 70 80 90 100
SFHDFLQLFD ETKVQYGLAR VSPPGSDVEK IIIIGWCPDS APLKTRASFA
110 120 130 140 150
ANFAAVANNL FKGYHVQVTA RDEDDLDENE LLMKISNAAG ARYSIQTSSK
160 170 180 190 200
QQGKASTPPV KKSFTPSKSP APVSKKEPVK TPSPAPAAKI SSRVNDNNDD
210 220 230 240 250
DDWNEPELKE RDFDQAPLKP NQSSYKPIGK IDLQKVIAEE KAKEDPRLVQ
260 270 280 290 300
KPTAAGSKID PSSDIANLKN ESKLKRDSEF NSFLGTTKPP SMTESSLKND
310 320 330 340 350
DDKVIKGFRN EKSPAQLWAE RKAKQNSGNA ETKAEAPKPE VPEDEPEGEP
360 370 380 390 400
DVKDLKSKFE GLAASEKEEE EMENKFAPPP KKSEPTIISP KPFSKPQEPV
410 420 430 440 450
KAEEAEQPKT DYKKIGNPLP GMHIEADNEE EPEENDDDWD DDEDEAAQPP
460 470 480 490 500
LPSRNVASGA PVQKEEPEQE EIAPSLPSRN SIPAPKQEEA PEQAPEEEIE
510 520 530 540 550
EEAEEAAPQL PSRSSAAPPP PPRRATPEKK PKENPWATAE YDYDAAEDNE
560 570 580 590
LTFVENDKII NIEFVDDDWW LGELEKDGSK GLFPSNYVSL GN
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 58 | L → S in CAA36075 (PubMed:2405279).Curated | 1 | |
Sequence conflicti | 312 | K → I in CAA36075 (PubMed:2405279).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X51780 Genomic DNA Translation: CAA36075.1 X59720 Genomic DNA Translation: CAA42253.1 BK006937 Genomic DNA Translation: DAA07557.1 |
PIRi | S19503 LLBY |
RefSeqi | NP_010012.1, NM_001178794.1 |
Genome annotation databases
EnsemblFungii | YCR088W_mRNA; YCR088W; YCR088W |
GeneIDi | 850450 |
KEGGi | sce:YCR088W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X51780 Genomic DNA Translation: CAA36075.1 X59720 Genomic DNA Translation: CAA42253.1 BK006937 Genomic DNA Translation: DAA07557.1 |
PIRi | S19503 LLBY |
RefSeqi | NP_010012.1, NM_001178794.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1HQZ | X-ray | 2.10 | 1/2/3/4/5/6/7/8/9 | 1-141 | [»] | |
1JO8 | X-ray | 1.30 | A | 535-592 | [»] | |
2K3B | NMR | - | A | 535-592 | [»] | |
2RPN | NMR | - | A | 535-592 | [»] | |
SMRi | P15891 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGridi | 31060, 163 interactors |
DIPi | DIP-534N |
IntActi | P15891, 50 interactors |
MINTi | P15891 |
STRINGi | 4932.YCR088W |
PTM databases
iPTMneti | P15891 |
Proteomic databases
MaxQBi | P15891 |
PaxDbi | P15891 |
PRIDEi | P15891 |
Genome annotation databases
EnsemblFungii | YCR088W_mRNA; YCR088W; YCR088W |
GeneIDi | 850450 |
KEGGi | sce:YCR088W |
Organism-specific databases
EuPathDBi | FungiDB:YCR088W |
SGDi | S000000684 ABP1 |
Phylogenomic databases
HOGENOMi | HOG000246671 |
InParanoidi | P15891 |
KOi | K20520 |
OMAi | YKPTKPH |
Enzyme and pathway databases
BioCyci | YEAST:G3O-29382-MONOMER |
Reactomei | R-SCE-6798695 Neutrophil degranulation R-SCE-8856828 Clathrin-mediated endocytosis |
Miscellaneous databases
EvolutionaryTracei | P15891 |
PROi | PR:P15891 |
Family and domain databases
CDDi | cd11961 SH3_Abp1_fungi_C2, 1 hit |
Gene3Di | 3.40.20.10, 1 hit |
InterProi | View protein in InterPro IPR035718 Abp1_fungi_SH3_C2 IPR002108 ADF-H IPR029006 ADF-H/Gelsolin-like_dom_sf IPR036028 SH3-like_dom_sf IPR001452 SH3_domain |
Pfami | View protein in Pfam PF00241 Cofilin_ADF, 1 hit PF00018 SH3_1, 1 hit |
PRINTSi | PR00452 SH3DOMAIN |
SMARTi | View protein in SMART SM00102 ADF, 1 hit SM00326 SH3, 1 hit |
SUPFAMi | SSF50044 SSF50044, 1 hit |
PROSITEi | View protein in PROSITE PS51263 ADF_H, 1 hit PS50002 SH3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ABP1_YEAST | |
Accessioni | P15891Primary (citable) accession number: P15891 Secondary accession number(s): D6VR88 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1990 |
Last sequence update: | January 23, 2007 | |
Last modified: | November 13, 2019 | |
This is version 196 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Complete proteome, Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - SIMILARITY comments
Index of protein domains and families - Yeast chromosome III
Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references