UniProtKB - P15874 (GRPE_BACSU)
Protein GrpE
grpE
Functioni
Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding.
UniRule annotationGO - Molecular functioni
- adenyl-nucleotide exchange factor activity Source: GO_Central
- chaperone binding Source: InterPro
- protein homodimerization activity Source: InterPro
- unfolded protein binding Source: GO_Central
GO - Biological processi
- protein folding Source: InterPro
Keywordsi
Molecular function | Chaperone |
Biological process | Stress response |
Enzyme and pathway databases
BioCyci | BSUB:BSU25480-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Protein GrpEUniRule annotationAlternative name(s): HSP-70 cofactorUniRule annotation |
Gene namesi | Name:grpEUniRule annotation Ordered Locus Names:BSU25480 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm Curated
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000113742 | 2 – 187 | Protein GrpEAdd BLAST | 186 |
Proteomic databases
jPOSTi | P15874 |
PaxDbi | P15874 |
PRIDEi | P15874 |
Interactioni
Subunit structurei
Homodimer.
UniRule annotationGO - Molecular functioni
- chaperone binding Source: InterPro
- protein homodimerization activity Source: InterPro
- unfolded protein binding Source: GO_Central
Protein-protein interaction databases
STRINGi | 224308.BSU25480 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 38 | DisorderedSequence analysisAdd BLAST | 38 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 13 – 33 | Acidic residuesSequence analysisAdd BLAST | 21 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0576, Bacteria |
InParanoidi | P15874 |
OMAi | YAYEKIA |
PhylomeDBi | P15874 |
Family and domain databases
CDDi | cd00446, GrpE, 1 hit |
Gene3Di | 2.30.22.10, 1 hit 3.90.20.20, 1 hit |
HAMAPi | MF_01151, GrpE, 1 hit |
InterProi | View protein in InterPro IPR000740, GrpE IPR013805, GrpE_coiled_coil IPR009012, GrpE_head |
PANTHERi | PTHR21237, PTHR21237, 1 hit |
Pfami | View protein in Pfam PF01025, GrpE, 1 hit |
PRINTSi | PR00773, GRPEPROTEIN |
SUPFAMi | SSF51064, SSF51064, 1 hit SSF58014, SSF58014, 1 hit |
PROSITEi | View protein in PROSITE PS01071, GRPE, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MSEEKQTVEQ NETEEQEIIE EQAAADEQQE ETNESELLQN QINELQGLLE
60 70 80 90 100
EKENKLLRVQ ADFENYKRRS RLEMEASQKY RSQNIVTDLL PALDSFERAL
110 120 130 140 150
QVEADNEQTK SLLQGMEMVH RQLVEALKKE GVEAIEAVGQ EFDPNLHQAV
160 170 180
MQAEDENYGS NIVVEEMQKG YKLKDRVIRP SMVKVNQ
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 2 | S → H in strain: IS58. | 1 | |
Natural varianti | 5 | K → T in strain: IS58. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X51477 Genomic DNA Translation: CAA35841.1 M84964 Genomic DNA Translation: AAA22527.1 D84432 Genomic DNA Translation: BAA12463.1 AL009126 Genomic DNA Translation: CAB14490.1 |
PIRi | S08418 |
RefSeqi | NP_390426.1, NC_000964.3 WP_003230005.1, NZ_JNCM01000036.1 |
Genome annotation databases
EnsemblBacteriai | CAB14490; CAB14490; BSU_25480 |
GeneIDi | 937846 |
KEGGi | bsu:BSU25480 |
PATRICi | fig|224308.179.peg.2769 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X51477 Genomic DNA Translation: CAA35841.1 M84964 Genomic DNA Translation: AAA22527.1 D84432 Genomic DNA Translation: BAA12463.1 AL009126 Genomic DNA Translation: CAB14490.1 |
PIRi | S08418 |
RefSeqi | NP_390426.1, NC_000964.3 WP_003230005.1, NZ_JNCM01000036.1 |
3D structure databases
SMRi | P15874 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 224308.BSU25480 |
Proteomic databases
jPOSTi | P15874 |
PaxDbi | P15874 |
PRIDEi | P15874 |
Protocols and materials databases
DNASUi | 937846 |
Genome annotation databases
EnsemblBacteriai | CAB14490; CAB14490; BSU_25480 |
GeneIDi | 937846 |
KEGGi | bsu:BSU25480 |
PATRICi | fig|224308.179.peg.2769 |
Phylogenomic databases
eggNOGi | COG0576, Bacteria |
InParanoidi | P15874 |
OMAi | YAYEKIA |
PhylomeDBi | P15874 |
Enzyme and pathway databases
BioCyci | BSUB:BSU25480-MONOMER |
Family and domain databases
CDDi | cd00446, GrpE, 1 hit |
Gene3Di | 2.30.22.10, 1 hit 3.90.20.20, 1 hit |
HAMAPi | MF_01151, GrpE, 1 hit |
InterProi | View protein in InterPro IPR000740, GrpE IPR013805, GrpE_coiled_coil IPR009012, GrpE_head |
PANTHERi | PTHR21237, PTHR21237, 1 hit |
Pfami | View protein in Pfam PF01025, GrpE, 1 hit |
PRINTSi | PR00773, GRPEPROTEIN |
SUPFAMi | SSF51064, SSF51064, 1 hit SSF58014, SSF58014, 1 hit |
PROSITEi | View protein in PROSITE PS01071, GRPE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GRPE_BACSU | |
Accessioni | P15874Primary (citable) accession number: P15874 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1990 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 138 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families