UniProtKB - P15823 (ADA1B_RAT)
Alpha-1B adrenergic receptor
Adra1b
Functioni
This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine (PE)-stimulated ERK signaling in cardiac myocytes (By similarity).
By similarityGO - Molecular functioni
- alpha1-adrenergic receptor activity Source: RGD
- G protein-coupled receptor activity Source: GO_Central
- protein heterodimerization activity Source: UniProtKB
GO - Biological processi
- adenylate cyclase-activating adrenergic receptor signaling pathway Source: GO_Central
- adult heart development Source: RGD
- behavioral response to cocaine Source: RGD
- blood vessel remodeling Source: RGD
- cell-cell signaling Source: GO_Central
- cell growth involved in cardiac muscle cell development Source: RGD
- glucose homeostasis Source: RGD
- locomotory behavior Source: RGD
- multicellular organism aging Source: RGD
- negative regulation of glycogen catabolic process Source: RGD
- negative regulation of Rho protein signal transduction Source: MGI
- neuron-glial cell signaling Source: RGD
- organ growth Source: RGD
- phospholipase C-activating G protein-coupled receptor signaling pathway Source: GO_Central
- positive regulation of cytosolic calcium ion concentration Source: RGD
- positive regulation of glycogen catabolic process Source: RGD
- positive regulation of heart rate by epinephrine-norepinephrine Source: RGD
- positive regulation of MAPK cascade Source: UniProtKB
- positive regulation of the force of heart contraction by epinephrine-norepinephrine Source: RGD
- positive regulation of vasoconstriction Source: GO_Central
- regulation of blood pressure Source: RGD
- regulation of cardiac muscle contraction Source: InterPro
- response to amphetamine Source: RGD
- response to estrogen Source: RGD
- response to hormone Source: RGD
- response to morphine Source: RGD
- response to steroid hormone Source: RGD
- response to xenobiotic stimulus Source: RGD
- vasoconstriction of artery involved in baroreceptor response to lowering of systemic arterial blood pressure Source: RGD
- visual learning Source: RGD
Keywordsi
Molecular function | G-protein coupled receptor, Receptor, Transducer |
Enzyme and pathway databases
Reactomei | R-RNO-390696, Adrenoceptors R-RNO-416476, G alpha (q) signalling events R-RNO-416482, G alpha (12/13) signalling events |
Names & Taxonomyi
Protein namesi | Recommended name: Alpha-1B adrenergic receptorAlternative name(s): Alpha-1B adrenoreceptor Short name: Alpha-1B adrenoceptor |
Gene namesi | Name:Adra1b |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2054, Adra1b |
Subcellular locationi
Nucleus
- Nucleus membrane By similarity; Multi-pass membrane protein By similarity
Cytoplasm and Cytosol
- Cytoplasm By similarity
Plasma membrane
- Cell membrane By similarity; Multi-pass membrane protein Sequence analysis
Other locations
- caveola By similarity
Note: Location at the nuclear membrane facilitates heterooligomerization and regulates ERK-mediated signaling in cardiac myocytes. Colocalizes with GNAQ, PLCB1 as well as LAP2 at the nuclear membrane of cardiac myocytes (By similarity).By similarity
Nucleus
- nuclear membrane Source: UniProtKB
- nucleus Source: UniProtKB
Plasma Membrane
- caveola Source: UniProtKB-SubCell
- integral component of plasma membrane Source: GO_Central
- T-tubule Source: RGD
Other locations
- cytoplasm Source: UniProtKB
- integral component of membrane Source: RGD
- intercalated disc Source: RGD
- membrane Source: RGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 45 | ExtracellularBy similarityAdd BLAST | 45 | |
Transmembranei | 46 – 70 | Helical; Name=1By similarityAdd BLAST | 25 | |
Topological domaini | 71 – 83 | CytoplasmicBy similarityAdd BLAST | 13 | |
Transmembranei | 84 – 105 | Helical; Name=2By similarityAdd BLAST | 22 | |
Topological domaini | 106 – 115 | ExtracellularBy similarity | 10 | |
Transmembranei | 116 – 141 | Helical; Name=3By similarityAdd BLAST | 26 | |
Topological domaini | 142 – 161 | CytoplasmicBy similarityAdd BLAST | 20 | |
Transmembranei | 162 – 182 | Helical; Name=4By similarityAdd BLAST | 21 | |
Topological domaini | 183 – 201 | ExtracellularBy similarityAdd BLAST | 19 | |
Transmembranei | 202 – 224 | Helical; Name=5By similarityAdd BLAST | 23 | |
Topological domaini | 225 – 295 | CytoplasmicBy similarityAdd BLAST | 71 | |
Transmembranei | 296 – 319 | Helical; Name=6By similarityAdd BLAST | 24 | |
Topological domaini | 320 – 326 | ExtracellularBy similarity | 7 | |
Transmembranei | 327 – 351 | Helical; Name=7By similarityAdd BLAST | 25 | |
Topological domaini | 352 – 515 | CytoplasmicBy similarityAdd BLAST | 164 |
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Membrane, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000069072 | 1 – 515 | Alpha-1B adrenergic receptorAdd BLAST | 515 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 10 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 24 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 34 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 118 ↔ 195 | PROSITE-ProRule annotation | ||
Modified residuei | 264 | PhosphothreonineBy similarity | 1 | |
Lipidationi | 365 | S-palmitoyl cysteineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, PhosphoproteinPTM databases
GlyGeni | P15823, 3 sites |
iPTMneti | P15823 |
PhosphoSitePlusi | P15823 |
Interactioni
Subunit structurei
Homo- and heterooligomer. Heterooligomerizes with ADRA1B homooligomers in cardiac myocytes.
Interacts with CAVIN4.
By similarityGO - Molecular functioni
- protein heterodimerization activity Source: UniProtKB
Protein-protein interaction databases
DIPi | DIP-59867N |
IntActi | P15823, 1 interactor |
Chemistry databases
BindingDBi | P15823 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 392 – 430 | DisorderedSequence analysisAdd BLAST | 39 | |
Regioni | 474 – 515 | DisorderedSequence analysisAdd BLAST | 42 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 368 – 378 | Nuclear localization signalBy similarityAdd BLAST | 11 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 405 – 423 | Polar residuesSequence analysisAdd BLAST | 19 | |
Compositional biasi | 481 – 502 | Polar residuesSequence analysisAdd BLAST | 22 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
InParanoidi | P15823 |
PhylomeDBi | P15823 |
Family and domain databases
InterProi | View protein in InterPro IPR002233, ADR_fam IPR001115, ADRA1B_rcpt IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM |
PANTHERi | PTHR24248:SF17, PTHR24248:SF17, 1 hit |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR01103, ADRENERGICR PR00556, ADRENRGCA1BR PR00237, GPCRRHODOPSN |
SMARTi | View protein in SMART SM01381, 7TM_GPCR_Srsx, 1 hit |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MNPDLDTGHN TSAPAHWGEL KDDNFTGPNQ TSSNSTLPQL DVTRAISVGL
60 70 80 90 100
VLGAFILFAI VGNILVILSV ACNRHLRTPT NYFIVNLAIA DLLLSFTVLP
110 120 130 140 150
FSATLEVLGY WVLGRIFCDI WAAVDVLCCT ASILSLCAIS IDRYIGVRYS
160 170 180 190 200
LQYPTLVTRR KAILALLSVW VLSTVISIGP LLGWKEPAPN DDKECGVTEE
210 220 230 240 250
PFYALFSSLG SFYIPLAVIL VMYCRVYIVA KRTTKNLEAG VMKEMSNSKE
260 270 280 290 300
LTLRIHSKNF HEDTLSSTKA KGHNPRSSIA VKLFKFSREK KAAKTLGIVV
310 320 330 340 350
GMFILCWLPF FIALPLGSLF STLKPPDAVF KVVFWLGYFN SCLNPIIYPC
360 370 380 390 400
SSKEFKRAFM RILGCQCRGG RRRRRRRRLG ACAYTYRPWT RGGSLERSQS
410 420 430 440 450
RKDSLDDSGS CMSGTQRTLP SASPSPGYLG RGTQPPVELC AFPEWKPGAL
460 470 480 490 500
LSLPEPPGRR GRLDSGPLFT FKLLGDPESP GTEGDTSNGG CDTTTDLANG
510
QPGFKSNMPL APGHF
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketQ6IRH4 | Q6IRH4_RAT | Alpha-1B adrenergic receptor | Adra1b | 515 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 114 – 116 | GRI → LSF in AAA63478 (PubMed:1706716).Curated | 3 | |
Sequence conflicti | 203 | Y → C (PubMed:2156222).Curated | 1 | |
Sequence conflicti | 203 | Y → C (PubMed:7989580).Curated | 1 | |
Sequence conflicti | 207 | S → C (PubMed:2156222).Curated | 1 | |
Sequence conflicti | 207 | S → C (PubMed:7989580).Curated | 1 | |
Sequence conflicti | 306 | C → S in AAA40647 (PubMed:8406017).Curated | 1 | |
Sequence conflicti | 415 – 416 | TQ → QK in CAA35934 (PubMed:2156222).Curated | 2 | |
Sequence conflicti | 440 | C → Y in AAA40647 (PubMed:8406017).Curated | 1 | |
Sequence conflicti | 484 – 486 | GDT → ATA in CAA35934 (PubMed:2156222).Curated | 3 | |
Sequence conflicti | 493 | T → S in AAA40647 (PubMed:8406017).Curated | 1 | |
Sequence conflicti | 511 | A → G in CAA35934 (PubMed:2156222).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X51585 mRNA Translation: CAA35934.1 M60655 mRNA Translation: AAA63478.1 L08610, L08609 Genomic DNA Translation: AAA40647.1 D32045 Genomic DNA Translation: BAA06806.1 |
PIRi | JC1525 |
Genome annotation databases
UCSCi | RGD:2054, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X51585 mRNA Translation: CAA35934.1 M60655 mRNA Translation: AAA63478.1 L08610, L08609 Genomic DNA Translation: AAA40647.1 D32045 Genomic DNA Translation: BAA06806.1 |
PIRi | JC1525 |
3D structure databases
AlphaFoldDBi | P15823 |
SMRi | P15823 |
ModBasei | Search... |
Protein-protein interaction databases
DIPi | DIP-59867N |
IntActi | P15823, 1 interactor |
Chemistry databases
BindingDBi | P15823 |
ChEMBLi | CHEMBL315 |
DrugCentrali | P15823 |
GuidetoPHARMACOLOGYi | 23 |
Protein family/group databases
GPCRDBi | Search... |
PTM databases
GlyGeni | P15823, 3 sites |
iPTMneti | P15823 |
PhosphoSitePlusi | P15823 |
Genome annotation databases
UCSCi | RGD:2054, rat |
Organism-specific databases
RGDi | 2054, Adra1b |
Phylogenomic databases
InParanoidi | P15823 |
PhylomeDBi | P15823 |
Enzyme and pathway databases
Reactomei | R-RNO-390696, Adrenoceptors R-RNO-416476, G alpha (q) signalling events R-RNO-416482, G alpha (12/13) signalling events |
Miscellaneous databases
PROi | PR:P15823 |
Family and domain databases
InterProi | View protein in InterPro IPR002233, ADR_fam IPR001115, ADRA1B_rcpt IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM |
PANTHERi | PTHR24248:SF17, PTHR24248:SF17, 1 hit |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR01103, ADRENERGICR PR00556, ADRENRGCA1BR PR00237, GPCRRHODOPSN |
SMARTi | View protein in SMART SM01381, 7TM_GPCR_Srsx, 1 hit |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ADA1B_RAT | |
Accessioni | P15823Primary (citable) accession number: P15823 Secondary accession number(s): Q63215 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1990 |
Last sequence update: | June 1, 1994 | |
Last modified: | May 25, 2022 | |
This is version 153 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- 7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries - SIMILARITY comments
Index of protein domains and families