UniProtKB - P15806 (TFE2_MOUSE)
Transcription factor E2-alpha
Tcf3
Functioni
GO - Molecular functioni
- bHLH transcription factor binding Source: MGI
- chromatin binding Source: MGI
- cis-regulatory region sequence-specific DNA binding Source: MGI
- DNA binding Source: UniProtKB
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: NTNU_SB
- DNA-binding transcription factor activity Source: MGI
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: MGI
- E-box binding Source: UniProtKB
- mitogen-activated protein kinase kinase kinase binding Source: MGI
- PDZ domain binding Source: MGI
- protein heterodimerization activity Source: UniProtKB
- protein homodimerization activity Source: UniProtKB
- repressing transcription factor binding Source: MGI
- RNA polymerase II activating transcription factor binding Source: MGI
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: NTNU_SB
- sequence-specific DNA binding Source: MGI
- transcription factor binding Source: UniProtKB
- vitamin D response element binding Source: MGI
GO - Biological processi
- B cell lineage commitment Source: UniProtKB
- cell development Source: MGI
- erythrocyte differentiation Source: MGI
- gastrulation Source: BHF-UCL
- histone H3 acetylation Source: MGI
- histone H4 acetylation Source: MGI
- immunoglobulin V(D)J recombination Source: MGI
- lymphocyte differentiation Source: MGI
- natural killer cell differentiation Source: MGI
- negative regulation of erythrocyte differentiation Source: MGI
- negative regulation of transcription by RNA polymerase II Source: MGI
- nervous system development Source: UniProtKB-KW
- Peyer's patch development Source: MGI
- positive regulation of B cell proliferation Source: UniProtKB
- positive regulation of cell cycle Source: UniProtKB
- positive regulation of DNA-binding transcription factor activity Source: UniProtKB
- positive regulation of gene expression Source: MGI
- positive regulation of neuron differentiation Source: UniProtKB
- positive regulation of protein tyrosine kinase activity Source: MGI
- positive regulation of signaling receptor activity Source: MGI
- positive regulation of transcription, DNA-templated Source: MGI
- positive regulation of transcription by RNA polymerase II Source: BHF-UCL
- protein stabilization Source: MGI
- regulation of G1/S transition of mitotic cell cycle Source: UniProtKB
- regulation of transcription, DNA-templated Source: MGI
- regulation of transcription by RNA polymerase II Source: MGI
- response to drug Source: MGI
- response to lipopolysaccharide Source: MGI
- T cell differentiation in thymus Source: MGI
- transcription by RNA polymerase II Source: MGI
Keywordsi
Molecular function | DNA-binding |
Biological process | Differentiation, Neurogenesis, Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-MMU-525793, Myogenesis [P15806-1] R-MMU-8939236, RUNX1 regulates transcription of genes involved in differentiation of HSCs [P15806-1] |
Names & Taxonomyi
Protein namesi | Recommended name: Transcription factor E2-alphaAlternative name(s): Immunoglobulin enhancer-binding factor E12/E47 Transcription factor 3 Short name: TCF-3 Transcription factor A1 |
Gene namesi | Name:Tcf3 Synonyms:Alf2, Me2, Tcfe2a |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:98510, Tcf3 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Cytosol
- cytosol Source: MGI
Nucleus
- nuclear chromatin Source: MGI
- nuclear euchromatin Source: MGI
- nuclear nucleosome Source: MGI
- nucleoplasm Source: MGI
- nucleus Source: MGI
Other locations
- cytoplasm Source: UniProtKB
- protein-containing complex Source: UniProtKB
- RNA polymerase II transcription regulator complex Source: NTNU_SB
- transcription regulator complex Source: BHF-UCL
Keywords - Cellular componenti
NucleusPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000127468 | 1 – 651 | Transcription factor E2-alphaAdd BLAST | 651 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Modified residuei | 135 | PhosphoserineBy similarity | 1 | ||
Modified residuei | 140 | PhosphoserineBy similarity | 1 | ||
Modified residuei | 353 | PhosphothreonineCombined sources | 1 | ||
Modified residuei | 357 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 369 | Omega-N-methylarginineCombined sources | 1 | ||
Modified residuei | 377 | PhosphoserineBy similarity | 1 | ||
Cross-linki | 496 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | |||
Modified residuei | 526 | PhosphoserineBy similarity | 1 | ||
Cross-linki | 622 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | |||
Isoform E47 (identifier: P15806-2) | |||||
Modified residuei | 528 | PhosphothreonineCombined sources | 1 | ||
Modified residuei | 533 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugationProteomic databases
jPOSTi | P15806 |
MaxQBi | P15806 |
PaxDbi | P15806 |
PRIDEi | P15806 |
PTM databases
iPTMneti | P15806 |
PhosphoSitePlusi | P15806 |
Expressioni
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000020167, Expressed in bone marrow and 291 other tissues |
Genevisiblei | P15806, MM |
Interactioni
Subunit structurei
Homodimer (PubMed:12196028). Heterodimer; efficient DNA binding requires dimerization with another bHLH protein (PubMed:15226298).
Forms a heterodimer with TWIST1 and TWIST2 (PubMed:7589808, PubMed:23395635).
Forms a heterodimer with NEUROD1; the heterodimer is inhibited in presence of ID2, but not NR0B2, to E-box element (PubMed:23395635, PubMed:18069799).
Forms a heterodimer with TCF15; the heterodimer binds E-box element (PubMed:15226298, PubMed:23395635).
Forms a heterodimer with MYOG; heterodimerization enhances MYOG DNA-binding and transcriptional activities (PubMed:12196028).
Forms a heterodimer with ATOH8; repress transcription of TCF3 and TCF3-NEUROG3 dimer-induced transactivation of E box-dependent promoters (PubMed:23938248).
Component of a nuclear TAL-1 complex composed at least of CBFA2T3, LDB1, TAL1 and TCF3 (PubMed:16407974).
Interacts with NEUROD2 (PubMed:18214987).
Interacts with EP300 (By similarity).
Interacts with PTF1A, TGFB1I1 and UBE2I (PubMed:9409784, PubMed:11318877, PubMed:16291758).
Interacts with BHLHA9 (By similarity).
By similarity11 PublicationsInteracts with RALGAPA1 (PubMed:12200424).
Interacts with FIGLA (By similarity).
By similarity1 PublicationBinary interactionsi
Hide detailsP15806
With | #Exp. | IntAct |
---|---|---|
Hand1 [Q64279] | 2 | EBI-81370,EBI-81361 |
Hand2 [Q61039] | 2 | EBI-81370,EBI-81388 |
Id2 [P41136] | 2 | EBI-81370,EBI-309167 |
Tal1 [P22091] | 4 | EBI-81370,EBI-8006437 |
Isoform E47 [P15806-2]
With | #Exp. | IntAct |
---|---|---|
Id2 [P41136] | 6 | EBI-413585,EBI-309167 |
GO - Molecular functioni
- bHLH transcription factor binding Source: MGI
- mitogen-activated protein kinase kinase kinase binding Source: MGI
- PDZ domain binding Source: MGI
- protein heterodimerization activity Source: UniProtKB
- protein homodimerization activity Source: UniProtKB
- repressing transcription factor binding Source: MGI
- RNA polymerase II activating transcription factor binding Source: MGI
- transcription factor binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 204015, 103 interactors |
CORUMi | P15806 |
DIPi | DIP-30940N |
IntActi | P15806, 15 interactors |
MINTi | P15806 |
STRINGi | 10090.ENSMUSP00000100979 |
Miscellaneous databases
RNActi | P15806, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P15806 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P15806 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 546 – 599 | bHLHPROSITE-ProRule annotationAdd BLAST | 54 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 387 – 422 | Leucine-zipperAdd BLAST | 36 |
Phylogenomic databases
eggNOGi | KOG3910, Eukaryota |
GeneTreei | ENSGT00940000157036 |
InParanoidi | P15806 |
PhylomeDBi | P15806 |
Family and domain databases
CDDi | cd00083, HLH, 1 hit |
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 10 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MMNQSQRMAP VGSDKELSDL LDFSMMFPLP VANGKSRPAS LGGTQFAGSG
60 70 80 90 100
LEDRPSSGSW GSSDQNSSSF DPSRTYSEGA HFSDSHSSLP PSTFLGAGLG
110 120 130 140 150
GKGSERNAYA TFGRDTSVGT LSQAGFLPGE LSLSSPGPLS PSGIKSSSQY
160 170 180 190 200
YPSFPSNPRR RAADGGLDTQ PKKVRKVPPG LPSSVYPPSS GDSYSRDAAA
210 220 230 240 250
YPSAKTPSSA YPSPFYVADG SLHPSAELWS TPSQVGFGPM LGDGSSPLPL
260 270 280 290 300
APGSSSVGSG TFGGLQQQDR MGYQLHGSEV NGSLPAVSSF SAAPGTYSGT
310 320 330 340 350
SGHTPPVSGA AAESLLGTRG TTASSSGDAL GKALASIYSP DHSSNNFSPS
360 370 380 390 400
PSTPVGSPQG LPGTSQWPRA GAPSALSPNY DAGLHGLSKM EDRLDEAIHV
410 420 430 440 450
LRSHAVGTAS DLHGLLPGHG ALTTSFTGPM SLGGRHAGLV GGSHPEEGLT
460 470 480 490 500
SGASLLHNHA SLPSQPSSLP DLSQRPPDSY SGLGRAGTTA GASEIKREEK
510 520 530 540 550
EDEEIASVAD AEEDKKDLKV PRTRTSPDED EDDLLPPEQK AEREKERRVA
560 570 580 590 600
NNARERLRVR DINEAFKELG RMCQLHLSSE KPQTKLLILH QAVAVILSLE
610 620 630 640 650
QQVRERNLNP KAACLKRREE EKVSGVVGDP QLALSAAHPG LGEAHNPAGH
L
The sequence of this isoform differs from the canonical sequence as follows:
527-598: PDEDEDDLLP...LHQAVAVILS → STDEVLSLEE...LQQAVQVILG
Computationally mapped potential isoform sequencesi
There are 10 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9PWE2 | E9PWE2_MOUSE | Transcription factor E2-alpha | Tcf3 | 653 | Annotation score: | ||
E9PWE4 | E9PWE4_MOUSE | Transcription factor E2-alpha | Tcf3 | 648 | Annotation score: | ||
E9PVV1 | E9PVV1_MOUSE | Transcription factor E2-alpha | Tcf3 | 650 | Annotation score: | ||
E9PVV2 | E9PVV2_MOUSE | Transcription factor E2-alpha | Tcf3 | 649 | Annotation score: | ||
A0A0R4J011 | A0A0R4J011_MOUSE | Transcription factor E2-alpha | Tcf3 | 649 | Annotation score: | ||
A0A0R4J014 | A0A0R4J014_MOUSE | Transcription factor E2-alpha | Tcf3 | 652 | Annotation score: | ||
E9PWE5 | E9PWE5_MOUSE | Transcription factor E2-alpha | Tcf3 | 648 | Annotation score: | ||
E9PWE3 | E9PWE3_MOUSE | Transcription factor E2-alpha | Tcf3 | 645 | Annotation score: | ||
F6T941 | F6T941_MOUSE | Transcription factor E2-alpha | Tcf3 | 638 | Annotation score: | ||
D3Z037 | D3Z037_MOUSE | Transcription factor E2-alpha | Tcf3 | 66 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 156 | S → N in AAK18618 (PubMed:11309385).Curated | 1 | |
Sequence conflicti | 164 | D → N in AAK18618 (PubMed:11309385).Curated | 1 | |
Sequence conflicti | 167 | L → LA in AAH18260 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 294 | P → T in BAB30842 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 633 | A → P in AAH06860 (PubMed:15489334).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 387 | L → LQ1 Publication | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_011354 | 527 – 598 | PDEDE…AVILS → STDEVLSLEEKDLRDRERRM ANNARERVRVRDINEAFREL GRMCQLHLKSDKAQTKLLIL QQAVQVILG in isoform E47. 4 PublicationsAdd BLAST | 72 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF352579 mRNA Translation: AAK18618.1 AK017617 mRNA Translation: BAB30842.1 AK038738 mRNA Translation: BAC30118.1 AK156265 mRNA Translation: BAE33647.1 BC006860 mRNA Translation: AAH06860.1 BC018260 mRNA Translation: AAH18260.1 X17500 mRNA Translation: CAA35541.1 D16631 mRNA Translation: BAA04057.1 D16632 mRNA Translation: BAA04058.1 D16633 mRNA Translation: BAA04059.1 D16634 mRNA Translation: BAA04060.1 D16635 mRNA Translation: BAA04061.1 D16636 mRNA Translation: BAA04062.1 D16637 mRNA Translation: BAA04063.1 D16638 mRNA Translation: BAA04064.1 D29919 mRNA Translation: BAA06218.1 |
CCDSi | CCDS24022.1 [P15806-2] CCDS48630.1 [P15806-1] |
PIRi | S08410 |
RefSeqi | NP_001157620.1, NM_001164148.1 NP_001157621.1, NM_001164149.1 [P15806-1] NP_001157623.1, NM_001164151.1 NP_035678.3, NM_011548.4 [P15806-2] |
Genome annotation databases
Ensembli | ENSMUST00000105345; ENSMUSP00000100982; ENSMUSG00000020167 [P15806-2] ENSMUST00000105346; ENSMUSP00000100983; ENSMUSG00000020167 [P15806-1] |
GeneIDi | 21423 |
KEGGi | mmu:21423 |
UCSCi | uc007gdg.2, mouse [P15806-2] uc007gdi.2, mouse [P15806-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF352579 mRNA Translation: AAK18618.1 AK017617 mRNA Translation: BAB30842.1 AK038738 mRNA Translation: BAC30118.1 AK156265 mRNA Translation: BAE33647.1 BC006860 mRNA Translation: AAH06860.1 BC018260 mRNA Translation: AAH18260.1 X17500 mRNA Translation: CAA35541.1 D16631 mRNA Translation: BAA04057.1 D16632 mRNA Translation: BAA04058.1 D16633 mRNA Translation: BAA04059.1 D16634 mRNA Translation: BAA04060.1 D16635 mRNA Translation: BAA04061.1 D16636 mRNA Translation: BAA04062.1 D16637 mRNA Translation: BAA04063.1 D16638 mRNA Translation: BAA04064.1 D29919 mRNA Translation: BAA06218.1 |
CCDSi | CCDS24022.1 [P15806-2] CCDS48630.1 [P15806-1] |
PIRi | S08410 |
RefSeqi | NP_001157620.1, NM_001164148.1 NP_001157621.1, NM_001164149.1 [P15806-1] NP_001157623.1, NM_001164151.1 NP_035678.3, NM_011548.4 [P15806-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2QL2 | X-ray | 2.50 | A/C | 547-606 | [»] | |
SMRi | P15806 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 204015, 103 interactors |
CORUMi | P15806 |
DIPi | DIP-30940N |
IntActi | P15806, 15 interactors |
MINTi | P15806 |
STRINGi | 10090.ENSMUSP00000100979 |
PTM databases
iPTMneti | P15806 |
PhosphoSitePlusi | P15806 |
Proteomic databases
jPOSTi | P15806 |
MaxQBi | P15806 |
PaxDbi | P15806 |
PRIDEi | P15806 |
Protocols and materials databases
Antibodypediai | 4332, 563 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000105345; ENSMUSP00000100982; ENSMUSG00000020167 [P15806-2] ENSMUST00000105346; ENSMUSP00000100983; ENSMUSG00000020167 [P15806-1] |
GeneIDi | 21423 |
KEGGi | mmu:21423 |
UCSCi | uc007gdg.2, mouse [P15806-2] uc007gdi.2, mouse [P15806-1] |
Organism-specific databases
CTDi | 6929 |
MGIi | MGI:98510, Tcf3 |
Phylogenomic databases
eggNOGi | KOG3910, Eukaryota |
GeneTreei | ENSGT00940000157036 |
InParanoidi | P15806 |
PhylomeDBi | P15806 |
Enzyme and pathway databases
Reactomei | R-MMU-525793, Myogenesis [P15806-1] R-MMU-8939236, RUNX1 regulates transcription of genes involved in differentiation of HSCs [P15806-1] |
Miscellaneous databases
BioGRID-ORCSi | 21423, 4 hits in 19 CRISPR screens |
ChiTaRSi | Tcf3, mouse |
EvolutionaryTracei | P15806 |
PROi | PR:P15806 |
RNActi | P15806, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000020167, Expressed in bone marrow and 291 other tissues |
Genevisiblei | P15806, MM |
Family and domain databases
CDDi | cd00083, HLH, 1 hit |
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TFE2_MOUSE | |
Accessioni | P15806Primary (citable) accession number: P15806 Secondary accession number(s): Q3U153 Q9CYJ4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1990 |
Last sequence update: | August 16, 2004 | |
Last modified: | February 10, 2021 | |
This is version 201 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references