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Protein

Shikimate dehydrogenase (NADP(+))

Gene

aroE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA). It displays no activity in the presence of NAD.UniRule annotation2 Publications

Catalytic activityi

Shikimate + NADP+ = 3-dehydroshikimate + NADPH.UniRule annotation1 Publication

Kineticsi

Kcat is 14.2 min(-1) for dehydrogenase activity with NADP or shikimate.1 Publication
  1. KM=56 µM for NADP1 Publication
  2. KM=65 µM for shikimate1 Publication

    Pathwayi: chorismate biosynthesis

    This protein is involved in step 4 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
    Proteins known to be involved in the 7 steps of the subpathway in this organism are:
    1. Phospho-2-dehydro-3-deoxyheptonate aldolase, Tyr-sensitive (aroF), Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive (aroH), Phospho-2-dehydro-3-deoxyheptonate aldolase, Phe-sensitive (aroG)
    2. 3-dehydroquinate synthase (aroB)
    3. 3-dehydroquinate dehydratase (aroD)
    4. Quinate/shikimate dehydrogenase (ydiB), Shikimate dehydrogenase (NADP(+)) (aroE)
    5. Shikimate kinase 2 (aroL), Shikimate kinase 1 (aroK)
    6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
    7. Chorismate synthase (aroC)
    This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei61ShikimateUniRule annotation1
    Active sitei65Proton acceptorUniRule annotation1
    Binding sitei77NADPUniRule annotation1
    Binding sitei86ShikimateUniRule annotation1
    Binding sitei102ShikimateUniRule annotation1
    Binding sitei213NADP; via carbonyl oxygenUniRule annotation1 Publication1
    Binding sitei215ShikimateUniRule annotation1
    Binding sitei237NADP; via carbonyl oxygenUniRule annotation1 Publication1
    Binding sitei244ShikimateUniRule annotation1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi126 – 130NADPUniRule annotation1 Publication5
    Nucleotide bindingi149 – 154NADPUniRule annotation1 Publication6

    GO - Molecular functioni

    • NADP binding Source: EcoCyc
    • nucleotide binding Source: EcoCyc
    • shikimate 3-dehydrogenase (NADP+) activity Source: EcoCyc

    GO - Biological processi

    • aromatic amino acid family biosynthetic process Source: UniProtKB-KW
    • chorismate biosynthetic process Source: EcoCyc
    • shikimate metabolic process Source: UniProtKB

    Keywordsi

    Molecular functionOxidoreductase
    Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis
    LigandNADP

    Enzyme and pathway databases

    BioCyciEcoCyc:AROE-MONOMER
    MetaCyc:AROE-MONOMER
    SABIO-RKiP15770
    UniPathwayiUPA00053; UER00087

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Shikimate dehydrogenase (NADP(+))1 Publication (EC:1.1.1.251 Publication)
    Short name:
    SD1 Publication
    Short name:
    SDHUniRule annotation
    Gene namesi
    Name:aroE1 PublicationUniRule annotation
    Ordered Locus Names:b3281, JW3242
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG10077 aroE

    Subcellular locationi

    GO - Cellular componenti

    • cytosol Source: EcoCyc

    Pathology & Biotechi

    Chemistry databases

    DrugBankiDB03461 2'-Monophosphoadenosine 5'-Diphosphoribose

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001360021 – 272Shikimate dehydrogenase (NADP(+))Add BLAST272

    Proteomic databases

    PaxDbiP15770
    PRIDEiP15770

    Interactioni

    Subunit structurei

    Monomer or homodimer.UniRule annotation2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    yedEP310642EBI-544087,EBI-544094

    Protein-protein interaction databases

    BioGridi4261870, 147 interactors
    DIPiDIP-9153N
    IntActiP15770, 6 interactors
    STRINGi316385.ECDH10B_3456

    Structurei

    Secondary structure

    1272
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi3 – 10Combined sources8
    Helixi16 – 27Combined sources12
    Beta strandi33 – 37Combined sources5
    Helixi43 – 52Combined sources10
    Beta strandi57 – 60Combined sources4
    Helixi65 – 71Combined sources7
    Beta strandi73 – 75Combined sources3
    Helixi77 – 82Combined sources6
    Beta strandi86 – 90Combined sources5
    Beta strandi96 – 99Combined sources4
    Helixi102 – 112Combined sources11
    Beta strandi121 – 125Combined sources5
    Helixi129 – 140Combined sources12
    Beta strandi144 – 148Combined sources5
    Helixi152 – 161Combined sources10
    Helixi163 – 165Combined sources3
    Beta strandi166 – 170Combined sources5
    Helixi174 – 176Combined sources3
    Beta strandi182 – 186Combined sources5
    Helixi191 – 193Combined sources3
    Helixi201 – 203Combined sources3
    Beta strandi209 – 213Combined sources5
    Helixi221 – 228Combined sources8
    Beta strandi233 – 235Combined sources3
    Helixi238 – 253Combined sources16
    Helixi259 – 270Combined sources12

    3D structure databases

    ProteinModelPortaliP15770
    SMRiP15770
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP15770

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni14 – 16Shikimate bindingUniRule annotation3

    Sequence similaritiesi

    Belongs to the shikimate dehydrogenase family.UniRule annotation

    Phylogenomic databases

    eggNOGiENOG4105E2X Bacteria
    COG0169 LUCA
    HOGENOMiHOG000237876
    InParanoidiP15770
    KOiK00014
    OMAiFGNPIKH
    PhylomeDBiP15770

    Family and domain databases

    HAMAPiMF_00222 Shikimate_DH_AroE, 1 hit
    InterProiView protein in InterPro
    IPR036291 NAD(P)-bd_dom_sf
    IPR011342 Shikimate_DH
    IPR013708 Shikimate_DH-bd_N
    IPR022893 Shikimate_DH_fam
    IPR006151 Shikm_DH/Glu-tRNA_Rdtase
    PfamiView protein in Pfam
    PF01488 Shikimate_DH, 1 hit
    PF08501 Shikimate_dh_N, 1 hit
    SUPFAMiSSF51735 SSF51735, 1 hit
    TIGRFAMsiTIGR00507 aroE, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P15770-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    METYAVFGNP IAHSKSPFIH QQFAQQLNIE HPYGRVLAPI NDFINTLNAF
    60 70 80 90 100
    FSAGGKGANV TVPFKEEAFA RADELTERAA LAGAVNTLMR LEDGRLLGDN
    110 120 130 140 150
    TDGVGLLSDL ERLSFIRPGL RILLIGAGGA SRGVLLPLLS LDCAVTITNR
    160 170 180 190 200
    TVSRAEELAK LFAHTGSIQA LSMDELEGHE FDLIINATSS GISGDIPAIP
    210 220 230 240 250
    SSLIHPGIYC YDMFYQKGKT PFLAWCEQRG SKRNADGLGM LVAQAAHAFL
    260 270
    LWHGVLPDVE PVIKQLQEEL SA
    Length:272
    Mass (Da):29,414
    Last modified:April 1, 1990 - v1
    Checksum:i62D3797ECA1EC1E8
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Y00710 Genomic DNA Translation: CAA68700.1
    U18997 Genomic DNA Translation: AAA58078.1
    U00096 Genomic DNA Translation: AAC76306.1
    AP009048 Genomic DNA Translation: BAE78010.1
    PIRiS00252
    RefSeqiNP_417740.1, NC_000913.3
    WP_000451243.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAC76306; AAC76306; b3281
    BAE78010; BAE78010; BAE78010
    GeneIDi947776
    KEGGiecj:JW3242
    eco:b3281
    PATRICifig|1411691.4.peg.3450

    Similar proteinsi

    Entry informationi

    Entry nameiAROE_ECOLI
    AccessioniPrimary (citable) accession number: P15770
    Secondary accession number(s): Q2M6U6
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: April 1, 1990
    Last modified: March 28, 2018
    This is version 164 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

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