UniProtKB - P15735 (PHKG2_HUMAN)
Phosphorylase b kinase gamma catalytic chain, liver/testis isoform
PHKG2
Functioni
Catalytic subunit of the phosphorylase b kinase (PHK), which mediates the neural and hormonal regulation of glycogen breakdown (glycogenolysis) by phosphorylating and thereby activating glycogen phosphorylase. May regulate glycogeneolysis in the testis. In vitro, phosphorylates PYGM (By similarity).
By similarity1 PublicationCatalytic activityi
- 2 ATP + phosphorylase b = 2 ADP + phosphorylase a. EC:2.7.11.19
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 53 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 153 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 30 – 38 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- calmodulin binding Source: UniProtKB-KW
- enzyme binding Source: Ensembl
- phosphorylase kinase activity Source: UniProtKB
- protein serine/threonine kinase activity Source: UniProtKB
- tau-protein kinase activity Source: UniProtKB
GO - Biological processi
- generation of precursor metabolites and energy Source: ProtInc
- glycogen biosynthetic process Source: InterPro
- glycogen metabolic process Source: ProtInc
- positive regulation of glycogen catabolic process Source: UniProtKB
- protein phosphorylation Source: ProtInc
Keywordsi
Molecular function | Calmodulin-binding, Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Carbohydrate metabolism, Glycogen metabolism |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.11.19, 2681 |
PathwayCommonsi | P15735 |
Reactomei | R-HSA-70221, Glycogen breakdown (glycogenolysis) |
SignaLinki | P15735 |
SIGNORi | P15735 |
Names & Taxonomyi
Protein namesi | Recommended name: Phosphorylase b kinase gamma catalytic chain, liver/testis isoform (EC:2.7.11.19)Short name: PHK-gamma-LT Short name: PHK-gamma-T Alternative name(s): PSK-C3 Phosphorylase kinase subunit gamma-2 |
Gene namesi | Name:PHKG2 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:8931, PHKG2 |
MIMi | 172471, gene |
neXtProti | NX_P15735 |
VEuPathDBi | HostDB:ENSG00000156873 |
Subcellular locationi
Cytosol
- cytosol Source: HPA
Other locations
- phosphorylase kinase complex Source: UniProtKB
Pathology & Biotechi
Involvement in diseasei
Glycogen storage disease 9C (GSD9C)3 Publications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_009517 | 106 | V → E in GSD9C. 1 PublicationCorresponds to variant dbSNP:rs137853589EnsemblClinVar. | 1 | |
Natural variantiVAR_020854 | 157 | E → K in GSD9C. 1 PublicationCorresponds to variant dbSNP:rs752961445EnsemblClinVar. | 1 | |
Natural variantiVAR_009518 | 189 | G → E in GSD9C. 1 PublicationCorresponds to variant dbSNP:rs137853588EnsemblClinVar. | 1 | |
Natural variantiVAR_020855 | 215 | D → N in GSD9C. 1 PublicationCorresponds to variant dbSNP:rs767427889EnsemblClinVar. | 1 |
Keywords - Diseasei
Disease variant, Glycogen storage diseaseOrganism-specific databases
DisGeNETi | 5261 |
GeneReviewsi | PHKG2 |
MalaCardsi | PHKG2 |
MIMi | 613027, phenotype |
OpenTargetsi | ENSG00000156873 |
Orphaneti | 264580, Glycogen storage disease due to liver phosphorylase kinase deficiency |
PharmGKBi | PA33272 |
Miscellaneous databases
Pharosi | P15735, Tchem |
Chemistry databases
ChEMBLi | CHEMBL2349 |
DrugCentrali | P15735 |
GuidetoPHARMACOLOGYi | 2147 |
Genetic variation databases
BioMutai | PHKG2 |
DMDMi | 125536 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000086512 | 1 – 406 | Phosphorylase b kinase gamma catalytic chain, liver/testis isoformAdd BLAST | 406 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 345 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | P15735 |
jPOSTi | P15735 |
MassIVEi | P15735 |
MaxQBi | P15735 |
PaxDbi | P15735 |
PeptideAtlasi | P15735 |
PRIDEi | P15735 |
ProteomicsDBi | 53208 [P15735-1] 53209 [P15735-2] |
PTM databases
GlyGeni | P15735, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | P15735 |
PhosphoSitePlusi | P15735 |
Expressioni
Gene expression databases
Bgeei | ENSG00000156873, Expressed in right testis and 181 other tissues |
ExpressionAtlasi | P15735, baseline and differential |
Genevisiblei | P15735, HS |
Organism-specific databases
HPAi | ENSG00000156873, Tissue enriched (testis) |
Interactioni
Subunit structurei
Hexadecamer of 4 heterotetramers, each composed of alpha, beta, gamma, and delta subunits. Alpha (PHKA1 or PHKA2) and beta (PHKB) are regulatory subunits, gamma (PHKG1 or PHKG2) is the catalytic subunit, and delta is calmodulin.
1 PublicationBinary interactionsi
GO - Molecular functioni
- calmodulin binding Source: UniProtKB-KW
- enzyme binding Source: Ensembl
Protein-protein interaction databases
BioGRIDi | 111279, 71 interactors |
IntActi | P15735, 53 interactors |
MINTi | P15735 |
STRINGi | 9606.ENSP00000455607 |
Chemistry databases
BindingDBi | P15735 |
Miscellaneous databases
RNActi | P15735, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P15735 |
SMRi | P15735 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 24 – 291 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 268 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 306 – 330 | Calmodulin-binding (domain-N)By similarityAdd BLAST | 25 | |
Regioni | 346 – 370 | Calmodulin-binding (domain-C)By similarityAdd BLAST | 25 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0599, Eukaryota |
GeneTreei | ENSGT00940000160435 |
InParanoidi | P15735 |
OrthoDBi | 330091at2759 |
PhylomeDBi | P15735 |
TreeFami | TF320349 |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR002291, Phosph_kin_gamma IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
PRINTSi | PR01049, PHOSPHBKNASE |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MTLDVGPEDE LPDWAAAKEF YQKYDPKDVI GRGVSSVVRR CVHRATGHEF
60 70 80 90 100
AVKIMEVTAE RLSPEQLEEV REATRRETHI LRQVAGHPHI ITLIDSYESS
110 120 130 140 150
SFMFLVFDLM RKGELFDYLT EKVALSEKET RSIMRSLLEA VSFLHANNIV
160 170 180 190 200
HRDLKPENIL LDDNMQIRLS DFGFSCHLEP GEKLRELCGT PGYLAPEILK
210 220 230 240 250
CSMDETHPGY GKEVDLWACG VILFTLLAGS PPFWHRRQIL MLRMIMEGQY
260 270 280 290 300
QFSSPEWDDR SSTVKDLISR LLQVDPEARL TAEQALQHPF FERCEGSQPW
310 320 330 340 350
NLTPRQRFRV AVWTVLAAGR VALSTHRVRP LTKNALLRDP YALRSVRHLI
360 370 380 390 400
DNCAFRLYGH WVKKGEQQNR AALFQHRPPG PFPIMGPEEE GDSAAITEDE
AVLVLG
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketJ3KNN3 | J3KNN3_HUMAN | Phosphorylase kinase | PHKG2 | 410 | Annotation score: | ||
H3BP07 | H3BP07_HUMAN | Phosphorylase b kinase gamma cataly... | PHKG2 | 254 | Annotation score: | ||
H3BTW6 | H3BTW6_HUMAN | Phosphorylase b kinase gamma cataly... | PHKG2 | 266 | Annotation score: | ||
H3BN16 | H3BN16_HUMAN | Phosphorylase b kinase gamma cataly... | PHKG2 | 87 | Annotation score: | ||
H3BTI9 | H3BTI9_HUMAN | Phosphorylase b kinase gamma cataly... | PHKG2 | 80 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 146 | A → P in AAA36518 (PubMed:2948189).Curated | 1 | |
Sequence conflicti | 176 | C → S in AAA36518 (PubMed:2948189).Curated | 1 | |
Sequence conflicti | 179 | E → D in AAA36518 (PubMed:2948189).Curated | 1 | |
Sequence conflicti | 212 – 214 | KEV → LVD in AAA36518 (PubMed:2948189).Curated | 3 | |
Sequence conflicti | 221 | V → E in AAA36518 (PubMed:2948189).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_009517 | 106 | V → E in GSD9C. 1 PublicationCorresponds to variant dbSNP:rs137853589EnsemblClinVar. | 1 | |
Natural variantiVAR_020854 | 157 | E → K in GSD9C. 1 PublicationCorresponds to variant dbSNP:rs752961445EnsemblClinVar. | 1 | |
Natural variantiVAR_009518 | 189 | G → E in GSD9C. 1 PublicationCorresponds to variant dbSNP:rs137853588EnsemblClinVar. | 1 | |
Natural variantiVAR_020855 | 215 | D → N in GSD9C. 1 PublicationCorresponds to variant dbSNP:rs767427889EnsemblClinVar. | 1 | |
Natural variantiVAR_051658 | 247 | E → G. Corresponds to variant dbSNP:rs34006569Ensembl. | 1 | |
Natural variantiVAR_040996 | 317 | A → T1 PublicationCorresponds to variant dbSNP:rs759992249Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_041858 | 362 – 374 | VKKGE…RAALF → IRKQWIGKLMACV in isoform 2. 1 PublicationAdd BLAST | 13 | |
Alternative sequenceiVSP_041859 | 375 – 406 | Missing in isoform 2. 1 PublicationAdd BLAST | 32 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M31606 mRNA Translation: AAA36442.1 Y11950, Y11951 Genomic DNA Translation: CAA72694.1 AK289492 mRNA Translation: BAF82181.1 AK293551 mRNA Translation: BAG57028.1 AC106886 Genomic DNA No translation available. BC002541 mRNA Translation: AAH02541.1 M14503 mRNA Translation: AAA36518.1 |
CCDSi | CCDS10690.1 [P15735-1] CCDS54002.1 [P15735-2] |
PIRi | A40069, KIHUCT |
RefSeqi | NP_000285.1, NM_000294.2 [P15735-1] NP_001165903.1, NM_001172432.1 [P15735-2] |
Genome annotation databases
Ensembli | ENST00000424889.7; ENSP00000388571.3; ENSG00000156873.16 [P15735-2] ENST00000563588.6; ENSP00000455607.1; ENSG00000156873.16 |
GeneIDi | 5261 |
KEGGi | hsa:5261 |
MANE-Selecti | ENST00000563588.6; ENSP00000455607.1; NM_000294.3; NP_000285.1 |
UCSCi | uc021tgo.3, human [P15735-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M31606 mRNA Translation: AAA36442.1 Y11950, Y11951 Genomic DNA Translation: CAA72694.1 AK289492 mRNA Translation: BAF82181.1 AK293551 mRNA Translation: BAG57028.1 AC106886 Genomic DNA No translation available. BC002541 mRNA Translation: AAH02541.1 M14503 mRNA Translation: AAA36518.1 |
CCDSi | CCDS10690.1 [P15735-1] CCDS54002.1 [P15735-2] |
PIRi | A40069, KIHUCT |
RefSeqi | NP_000285.1, NM_000294.2 [P15735-1] NP_001165903.1, NM_001172432.1 [P15735-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2Y7J | X-ray | 2.50 | A/B/C/D | 6-293 | [»] | |
AlphaFoldDBi | P15735 | |||||
SMRi | P15735 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 111279, 71 interactors |
IntActi | P15735, 53 interactors |
MINTi | P15735 |
STRINGi | 9606.ENSP00000455607 |
Chemistry databases
BindingDBi | P15735 |
ChEMBLi | CHEMBL2349 |
DrugCentrali | P15735 |
GuidetoPHARMACOLOGYi | 2147 |
PTM databases
GlyGeni | P15735, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | P15735 |
PhosphoSitePlusi | P15735 |
Genetic variation databases
BioMutai | PHKG2 |
DMDMi | 125536 |
Proteomic databases
EPDi | P15735 |
jPOSTi | P15735 |
MassIVEi | P15735 |
MaxQBi | P15735 |
PaxDbi | P15735 |
PeptideAtlasi | P15735 |
PRIDEi | P15735 |
ProteomicsDBi | 53208 [P15735-1] 53209 [P15735-2] |
Protocols and materials databases
Antibodypediai | 13792, 377 antibodies from 30 providers |
DNASUi | 5261 |
Genome annotation databases
Ensembli | ENST00000424889.7; ENSP00000388571.3; ENSG00000156873.16 [P15735-2] ENST00000563588.6; ENSP00000455607.1; ENSG00000156873.16 |
GeneIDi | 5261 |
KEGGi | hsa:5261 |
MANE-Selecti | ENST00000563588.6; ENSP00000455607.1; NM_000294.3; NP_000285.1 |
UCSCi | uc021tgo.3, human [P15735-1] |
Organism-specific databases
CTDi | 5261 |
DisGeNETi | 5261 |
GeneCardsi | PHKG2 |
GeneReviewsi | PHKG2 |
HGNCi | HGNC:8931, PHKG2 |
HPAi | ENSG00000156873, Tissue enriched (testis) |
MalaCardsi | PHKG2 |
MIMi | 172471, gene 613027, phenotype |
neXtProti | NX_P15735 |
OpenTargetsi | ENSG00000156873 |
Orphaneti | 264580, Glycogen storage disease due to liver phosphorylase kinase deficiency |
PharmGKBi | PA33272 |
VEuPathDBi | HostDB:ENSG00000156873 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0599, Eukaryota |
GeneTreei | ENSGT00940000160435 |
InParanoidi | P15735 |
OrthoDBi | 330091at2759 |
PhylomeDBi | P15735 |
TreeFami | TF320349 |
Enzyme and pathway databases
BRENDAi | 2.7.11.19, 2681 |
PathwayCommonsi | P15735 |
Reactomei | R-HSA-70221, Glycogen breakdown (glycogenolysis) |
SignaLinki | P15735 |
SIGNORi | P15735 |
Miscellaneous databases
BioGRID-ORCSi | 5261, 9 hits in 1114 CRISPR screens |
ChiTaRSi | PHKG2, human |
GeneWikii | PHKG2 |
GenomeRNAii | 5261 |
Pharosi | P15735, Tchem |
PROi | PR:P15735 |
RNActi | P15735, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000156873, Expressed in right testis and 181 other tissues |
ExpressionAtlasi | P15735, baseline and differential |
Genevisiblei | P15735, HS |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR002291, Phosph_kin_gamma IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
PRINTSi | PR01049, PHOSPHBKNASE |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PHKG2_HUMAN | |
Accessioni | P15735Primary (citable) accession number: P15735 Secondary accession number(s): A8K0C7 P11800 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1990 |
Last sequence update: | April 1, 1990 | |
Last modified: | May 25, 2022 | |
This is version 224 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human and mouse protein kinases
Human and mouse protein kinases: classification and index - Human chromosome 16
Human chromosome 16: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families