UniProtKB - P15709 (ST2A1_RAT)
Protein
Sulfotransferase 2A1
Gene
Sult2a1
Organism
Rattus norvegicus (Rat)
Status
Functioni
Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfonation of steroids and bile acids in the liver and adrenal glands (By similarity). Mediates the sulfation of a wide range of steroids and sterols, including pregnenolone, androsterone, DHEA, bile acids, cholesterol and as well many xenobiotics that contain alcohol and phenol functional groups (PubMed:7854148). Sulfonation increases the water solubility of most compounds, and therefore their renal excretion, but it can also result in bioactivation to form active metabolites. Plays an important role in maintening steroid and lipid homeostasis. Plays a key role in bile acid metabolism (By similarity). In addition, catalyzes the metabolic activation of potent carcinogenic polycyclic arylmethanols (PubMed:7854148).By similarity1 Publication
Catalytic activityi
- 3'-phosphoadenylyl sulfate + an alcohol = adenosine 3',5'-bisphosphate + an alkyl sulfate + H+By similarityEC:2.8.2.2By similarityThis reaction proceeds in the forwardBy similarity direction.
- 3'-phosphoadenylyl sulfate + taurolithocholate = adenosine 3',5'-bisphosphate + H+ + taurolithocholate sulfateBy similarityEC:2.8.2.14By similarityThis reaction proceeds in the forwardBy similarity direction.
- 3'-phosphoadenylyl sulfate + lithocholate = adenosine 3',5'-bisphosphate + H+ + lithocholate sulfateBy similarityThis reaction proceeds in the forwardBy similarity direction.
- (24S)-hydroxycholesterol + 3'-phosphoadenylyl sulfate = (24S)-hydroxycholesterol 24-sulfate + adenosine 3',5'-bisphosphate + H+By similarityThis reaction proceeds in the forwardBy similarity direction.
- (24S)-hydroxycholesterol + 3'-phosphoadenylyl sulfate = (24S)-hydroxycholesterol 3-sulfate + adenosine 3',5'-bisphosphate + H+By similarityThis reaction proceeds in the forwardBy similarity direction.
- (24S)-hydroxycholesterol 24-sulfate + 3'-phosphoadenylyl sulfate = (24S)-hydroxycholesterol 3,24-disulfate + adenosine 3',5'-bisphosphate + H+By similarityThis reaction proceeds in the forwardBy similarity direction.
- 3'-phosphoadenylyl sulfate + 3β-hydroxyandrost-5-en-17-one = 3β-sulfooxy-androst-5-en-17-one + adenosine 3',5'-bisphosphate + H+By similarityThis reaction proceeds in the forwardBy similarity direction.
- 3'-phosphoadenylyl sulfate + pregnenolone = adenosine 3',5'-bisphosphate + H+ + pregnenolone sulfateBy similarityThis reaction proceeds in the forwardBy similarity direction.
- 3'-phosphoadenylyl sulfate + 3α-hydroxy-5α-androstan-17-one = 3α-sulfooxy-5α-androstan-17-one + adenosine 3',5'-bisphosphate + H+By similarityThis reaction proceeds in the forwardBy similarity direction.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 98 | Proton acceptorBy similarity | 1 | |
Binding sitei | 120 | PAPSBy similarity | 1 | |
Binding sitei | 128 | PAPSBy similarity | 1 | |
Binding sitei | 183 | PAPSBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 43 – 48 | PAPSBy similarity | 6 | |
Nucleotide bindingi | 217 – 222 | PAPSBy similarity | 6 | |
Nucleotide bindingi | 246 – 248 | PAPSBy similarity | 3 |
GO - Molecular functioni
- bile-salt sulfotransferase activity Source: UniProtKB-EC
GO - Biological processi
- steroid metabolic process Source: UniProtKB-KW
Keywordsi
Molecular function | Transferase |
Biological process | Lipid metabolism, Steroid metabolism |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Sult2a1 Synonyms:St2a1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 621337, Sult2a1 |
Subcellular locationi
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000085144 | 1 – 284 | Sulfotransferase 2A1Add BLAST | 284 |
Proteomic databases
PaxDbi | P15709 |
PRIDEi | P15709 |
PTM databases
iPTMneti | P15709 |
PhosphoSitePlusi | P15709 |
Expressioni
Developmental stagei
There is a marked sex difference (female >> male) in the expressed level of mRNA for this protein.1 Publication
Inductioni
Induced by estrogens and suppressed by androgens. Expression is under the influence of pituitary growth hormone and thyroid hormone.
Gene expression databases
Bgeei | ENSRNOG00000047986, Expressed in liver and 19 other tissues |
ExpressionAtlasi | A0A0G2JYC0, baseline and differential |
Interactioni
Subunit structurei
Homodimer.
By similarityProtein-protein interaction databases
STRINGi | 10116.ENSRNOP00000043445 |
Chemistry databases
BindingDBi | P15709 |
Family & Domainsi
Sequence similaritiesi
Belongs to the sulfotransferase 1 family.Curated
Phylogenomic databases
eggNOGi | KOG1584, Eukaryota |
GeneTreei | ENSGT00940000154432 |
InParanoidi | P15709 |
OrthoDBi | 780670at2759 |
PhylomeDBi | P15709 |
Family and domain databases
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR000863, Sulfotransferase_dom |
Pfami | View protein in Pfam PF00685, Sulfotransfer_1, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
P15709-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPDYTWFEGI PFHAFGISKE TLQNVCNKFV VKDEDLILLA YPKSGTNWLI
60 70 80 90 100
EIVCLIQTKG DPKWIQSVTI WDRSPWIETD LGYDMLIKKK GPRLITSHLP
110 120 130 140 150
MHLFSKSLFS SKAKVIYLVR NPRDVLVSGY YFWGNSTLAK KPDSLGTYVE
160 170 180 190 200
WFLKGNVLYG SWFEHIRAWL SMREWDNFLL LYYEDMKKDT MGTIKKICDF
210 220 230 240 250
LGKKLEPDEL DLVLKYSSFQ VMKENDMSNY SLLMKKSIFT GIGLMRKGTV
260 270 280
GDWKNHFTVS QAEAFDKVFQ EKMAGFPPGM FPWE
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF1M7G5 | F1M7G5_RAT | Sulfotransferase | Sult2a6 LOC100364224, Sult2a1, Sult2a2 | 284 | Annotation score: | ||
D4AE35 | D4AE35_RAT | Sulfotransferase | Sult2a1 Sult2a2, Sult2a6 | 285 | Annotation score: | ||
D3ZR55 | D3ZR55_RAT | Sulfotransferase | Sult2a2 Sult2a1, Sult2a6 | 284 | Annotation score: | ||
M3ZCQ0 | M3ZCQ0_RAT | Sulfotransferase | Sult2a1 Sult2a2 | 284 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 33 | D → E in BAA03633 (PubMed:8033273). | 1 | |
Sequence conflicti | 33 | D → E in BAA03632 (PubMed:8033273). | 1 | |
Sequence conflicti | 81 – 85 | LGYDM → VGYDI in AAA41356 (PubMed:2590219).Curated | 5 | |
Sequence conflicti | 81 – 85 | LGYDM → VGYDI in BAA03633 (PubMed:8033273).Curated | 5 | |
Sequence conflicti | 81 – 85 | LGYDM → VGYDI in BAA03632 (PubMed:8033273).Curated | 5 | |
Sequence conflicti | 95 | I → M in AAA41356 (PubMed:2590219).Curated | 1 | |
Sequence conflicti | 95 | I → M in BAA03633 (PubMed:8033273).Curated | 1 | |
Sequence conflicti | 95 | I → M in BAA03632 (PubMed:8033273).Curated | 1 | |
Sequence conflicti | 119 | V → I in BAA03633 (PubMed:8033273).Curated | 1 | |
Sequence conflicti | 119 | V → I in BAA03632 (PubMed:8033273).Curated | 1 | |
Sequence conflicti | 139 | A → V in BAA03633 (PubMed:8033273).Curated | 1 | |
Sequence conflicti | 139 | A → V in BAA03632 (PubMed:8033273).Curated | 1 | |
Sequence conflicti | 173 | R → Q in AAA41356 (PubMed:2590219).Curated | 1 | |
Sequence conflicti | 242 | I → T in AAA41356 (PubMed:2590219).Curated | 1 | |
Sequence conflicti | 250 | V → I in BAA03633 (PubMed:8033273).Curated | 1 | |
Sequence conflicti | 250 | V → I in BAA03632 (PubMed:8033273).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M31363 mRNA Translation: AAA41356.1 D14988 mRNA Translation: BAA03633.1 D14987 mRNA Translation: BAA03632.1 AABR07002517 Genomic DNA No translation available. AABR07002526 Genomic DNA No translation available. AABR07002525 Genomic DNA No translation available. AABR07002524 Genomic DNA No translation available. AABR07002523 Genomic DNA No translation available. AABR07002522 Genomic DNA No translation available. AABR07002521 Genomic DNA No translation available. AABR07002520 Genomic DNA No translation available. AABR07002519 Genomic DNA No translation available. AABR07002518 Genomic DNA No translation available. |
PIRi | A33569 I52849 |
RefSeqi | NP_571978.1, NM_131903.1 |
Genome annotation databases
Ensembli | ENSRNOT00000089371; ENSRNOP00000070596; ENSRNOG00000047986 |
GeneIDi | 24912 |
KEGGi | rno:24912 |
UCSCi | RGD:621337, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M31363 mRNA Translation: AAA41356.1 D14988 mRNA Translation: BAA03633.1 D14987 mRNA Translation: BAA03632.1 AABR07002517 Genomic DNA No translation available. AABR07002526 Genomic DNA No translation available. AABR07002525 Genomic DNA No translation available. AABR07002524 Genomic DNA No translation available. AABR07002523 Genomic DNA No translation available. AABR07002522 Genomic DNA No translation available. AABR07002521 Genomic DNA No translation available. AABR07002520 Genomic DNA No translation available. AABR07002519 Genomic DNA No translation available. AABR07002518 Genomic DNA No translation available. |
PIRi | A33569 I52849 |
RefSeqi | NP_571978.1, NM_131903.1 |
3D structure databases
SMRi | P15709 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000043445 |
Chemistry databases
BindingDBi | P15709 |
ChEMBLi | CHEMBL3007 |
PTM databases
iPTMneti | P15709 |
PhosphoSitePlusi | P15709 |
Proteomic databases
PaxDbi | P15709 |
PRIDEi | P15709 |
Genome annotation databases
Ensembli | ENSRNOT00000089371; ENSRNOP00000070596; ENSRNOG00000047986 |
GeneIDi | 24912 |
KEGGi | rno:24912 |
UCSCi | RGD:621337, rat |
Organism-specific databases
CTDi | 6822 |
RGDi | 621337, Sult2a1 |
Phylogenomic databases
eggNOGi | KOG1584, Eukaryota |
GeneTreei | ENSGT00940000154432 |
InParanoidi | P15709 |
OrthoDBi | 780670at2759 |
PhylomeDBi | P15709 |
Miscellaneous databases
PROi | PR:P15709 |
Gene expression databases
Bgeei | ENSRNOG00000047986, Expressed in liver and 19 other tissues |
ExpressionAtlasi | A0A0G2JYC0, baseline and differential |
Family and domain databases
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR000863, Sulfotransferase_dom |
Pfami | View protein in Pfam PF00685, Sulfotransfer_1, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ST2A1_RAT | |
Accessioni | P15709Primary (citable) accession number: P15709 Secondary accession number(s): A0A0G2JYC0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1990 |
Last sequence update: | December 2, 2020 | |
Last modified: | December 2, 2020 | |
This is version 142 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families