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Protein

Leukosialin

Gene

Spn

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Predominant cell surface sialoprotein of leukocytes which regulates multiple T-cell functions, including T-cell activation, proliferation, differentiation, trafficking and migration. Positively regulates T-cell trafficking to lymph-nodes via its association with ERM proteins (EZR, RDX and MSN) (PubMed:17638845, PubMed:21289089, PubMed:11728336). Negatively regulates Th2 cell differentiation and predisposes the differentiation of T-cells towards a Th1 lineage commitment (PubMed:18490738). Promotes the expression of IFN-gamma by T-cells during T-cell receptor (TCR) activation of naive cells and induces the expression of IFN-gamma by CD4+ T-cells and to a lesser extent by CD8+ T-cells. Plays a role in preparing T-cells for cytokine sensing and differentiation into effector cells by inducing the expression of cytokine receptors IFNGR and IL4R, promoting IFNGR and IL4R signaling and by mediating the clustering of IFNGR with TCR (By similarity). Acts as a major E-selectin ligand responsible for Th17 cell rolling on activated vasculature and recruitment during inflammation. Mediates Th17 cells, but not Th1 cells, adhesion to E-selectin (PubMed:26700769). Acts as a T-cell counter-receptor for SIGLEC1 (PubMed:11238599).By similarity6 Publications
CD43 cytoplasmic tail: Protects cells from apoptotic signals, promoting cell survival.1 Publication

GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-MMU-202733 Cell surface interactions at the vascular wall
R-MMU-210991 Basigin interactions

Names & Taxonomyi

Protein namesi
Recommended name:
Leukosialin
Alternative name(s):
B-cell differentiation antigen LP-3
Leukocyte sialoglycoprotein
Lymphocyte antigen 48
Short name:
Ly-48
Sialophorin
CD_antigen: CD43
Cleaved into the following chain:
CD43 cytoplasmic tail1 Publication
Short name:
CD43-ct1 Publication
Short name:
CD43ct1 Publication
Gene namesi
Name:Spn
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:98384 Spn

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini20 – 248ExtracellularSequence analysisAdd BLAST229
Transmembranei249 – 271HelicalSequence analysisAdd BLAST23
Topological domaini272 – 395CytoplasmicSequence analysisAdd BLAST124

Keywords - Cellular componenti

Cell projection, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi276 – 278KRR → NGG: Loss of phosphorylation, interaction with EZR and localization to the uropodium. 1 Publication3
Mutagenesisi343S → A: Reduced phosphorylation. Significant loss of phosphorylation; when associated with A-347. 1 Publication1
Mutagenesisi347S → A: Reduced phosphorylation. Significant loss of phosphorylation; when associated with A-343. 2 Publications1
Mutagenesisi347S → D: No loss of phosphorylation or localization to the uropodium. Loss of interaction with EZR. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Add BLAST19
ChainiPRO_000002158920 – 395LeukosialinAdd BLAST376
ChainiPRO_0000443407272 – 395CD43 cytoplasmic tail1 PublicationAdd BLAST124

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi167N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei285PhosphoserineBy similarity1
Modified residuei328PhosphoserineBy similarity1
Modified residuei333PhosphothreonineBy similarity1
Modified residuei339PhosphoserineCombined sources1
Modified residuei343PhosphoserineCombined sources1 Publication1
Modified residuei347Phosphoserine; by PKC/PRKCQCombined sources1 Publication1
Modified residuei371PhosphoserineCombined sources1
Modified residuei378PhosphothreonineCombined sources1

Post-translational modificationi

Phosphorylation at Ser-347 is regulated by chemokines, requires its association with ERM proteins (EZR, RDX and MSN) and is essential for its function in the regulation of T-cell trafficking to lymph nodes.2 Publications
Has a high content of sialic acid and O-linked carbohydrate structures.
Cleavage by CTSG releases its extracellular domain and triggers its intramembrane proteolysis by gamma-secretase releasing the CD43 cytoplasmic tail chain (CD43-ct) which translocates to the nucleus.1 Publication
CD43 cytoplasmic tail: Sumoylated.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP15702
PaxDbiP15702
PeptideAtlasiP15702
PRIDEiP15702

PTM databases

iPTMnetiP15702
PhosphoSitePlusiP15702

Expressioni

Tissue specificityi

Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas.

Gene expression databases

BgeeiENSMUSG00000051457 Expressed in 206 organ(s), highest expression level in mesenteric lymph node
CleanExiMM_SPN
ExpressionAtlasiP15702 baseline and differential
GenevisibleiP15702 MM

Interactioni

Subunit structurei

Interacts with HIPK2 via the cytoplasmic domain (PubMed:11078605). Interacts with SIGLEC1 (PubMed:11238599). Interacts with RDX (PubMed:18614175). Interacts with EZR (PubMed:21289089). Interacts with MSN (By similarity).By similarity4 Publications

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000049534

Structurei

Secondary structure

1395
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP15702
SMRiP15702
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP15702

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni272 – 302Required for interaction with EZR, MSN and RDX and for co-localization to microvilliBy similarityAdd BLAST31

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410J161 Eukaryota
ENOG410YXQE LUCA
GeneTreeiENSGT00390000017626
HOGENOMiHOG000294199
HOVERGENiHBG006258
InParanoidiP15702
KOiK06477
OMAiTGPPVTM
OrthoDBiEOG091G0MEV
PhylomeDBiP15702
TreeFamiTF337688

Family and domain databases

InterProiView protein in InterPro
IPR038829 Leukosialin
PANTHERiPTHR35265 PTHR35265, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P15702-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MALHLLLLFG ACWVQVASPD SLQRTTMLPS TPHITAPSTS EAQNASPSVS
60 70 80 90 100
VGSGTVDSKE TISPWGQTTI PVSLTPLETT ELSSLETSAG ASMSTPVPEP
110 120 130 140 150
TASQEVSSKT SALLPEPSNV ASDPPVTAAN PVTDGPAANP VTDGTAASTS
160 170 180 190 200
ISKGTSAPPT TVTTSSNETS GPSVATTVSS KTSGPPVTTA TGSLGPSSEM
210 220 230 240 250
HGLPATTATS SVESSSVARG TSVSSRKTST TSTQDPITTR SPSQESSGML
260 270 280 290 300
LVPMLIALVV VLALVALLLL WRQRQKRRTG ALTLSGGGKR NGVVDAWAGP
310 320 330 340 350
ARVPDEEATT TSGAGGNKGS EVLETEGSGQ RPTLTTFFSR RKSRQGSLVL
360 370 380 390
EELKPGSGPN LKGEEEPLVG SEDEAVETPT SDGPQAKDEA APQSL
Length:395
Mass (Da):40,038
Last modified:April 1, 1990 - v1
Checksum:i369F201B04DBC055
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0U1RNN5A0A0U1RNN5_MOUSE
Leukosialin
Spn
140Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17018 Genomic DNA Translation: CAA34884.1
X52609 Genomic DNA Translation: CAA36840.1
S70677 mRNA Translation: AAB30765.1
M30693 mRNA Translation: AAA39457.1
CCDSiCCDS21858.1
PIRiA43545
RefSeqiNP_001032899.1, NM_001037810.2
NP_033285.1, NM_009259.5
XP_006507585.1, XM_006507522.3
UniGeneiMm.283714

Genome annotation databases

EnsembliENSMUST00000049931; ENSMUSP00000049534; ENSMUSG00000051457
ENSMUST00000143713; ENSMUSP00000122787; ENSMUSG00000051457
GeneIDi20737
KEGGimmu:20737
UCSCiuc009juh.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17018 Genomic DNA Translation: CAA34884.1
X52609 Genomic DNA Translation: CAA36840.1
S70677 mRNA Translation: AAB30765.1
M30693 mRNA Translation: AAA39457.1
CCDSiCCDS21858.1
PIRiA43545
RefSeqiNP_001032899.1, NM_001037810.2
NP_033285.1, NM_009259.5
XP_006507585.1, XM_006507522.3
UniGeneiMm.283714

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2EMSX-ray2.90B272-291[»]
ProteinModelPortaliP15702
SMRiP15702
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000049534

PTM databases

iPTMnetiP15702
PhosphoSitePlusiP15702

Proteomic databases

EPDiP15702
PaxDbiP15702
PeptideAtlasiP15702
PRIDEiP15702

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000049931; ENSMUSP00000049534; ENSMUSG00000051457
ENSMUST00000143713; ENSMUSP00000122787; ENSMUSG00000051457
GeneIDi20737
KEGGimmu:20737
UCSCiuc009juh.2 mouse

Organism-specific databases

CTDi6693
MGIiMGI:98384 Spn

Phylogenomic databases

eggNOGiENOG410J161 Eukaryota
ENOG410YXQE LUCA
GeneTreeiENSGT00390000017626
HOGENOMiHOG000294199
HOVERGENiHBG006258
InParanoidiP15702
KOiK06477
OMAiTGPPVTM
OrthoDBiEOG091G0MEV
PhylomeDBiP15702
TreeFamiTF337688

Enzyme and pathway databases

ReactomeiR-MMU-202733 Cell surface interactions at the vascular wall
R-MMU-210991 Basigin interactions

Miscellaneous databases

EvolutionaryTraceiP15702
PROiPR:P15702
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000051457 Expressed in 206 organ(s), highest expression level in mesenteric lymph node
CleanExiMM_SPN
ExpressionAtlasiP15702 baseline and differential
GenevisibleiP15702 MM

Family and domain databases

InterProiView protein in InterPro
IPR038829 Leukosialin
PANTHERiPTHR35265 PTHR35265, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiLEUK_MOUSE
AccessioniPrimary (citable) accession number: P15702
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: September 12, 2018
This is version 155 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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