UniProtKB - P15659 (PA_I33A0)
Protein
Polymerase acidic protein
Gene
PA
Organism
Influenza A virus (strain A/Wilson-Smith/1933 H1N1) (Influenza A virus (strain A/WS/1933 H1N1))
Status
Functioni
Plays an essential role in viral RNA transcription and replication by forming the heterotrimeric polymerase complex together with PB1 and PB2 subunits. The complex transcribes viral mRNAs by using a unique mechanism called cap-snatching. It consists in the hijacking and cleavage of host capped pre-mRNAs. These short capped RNAs are then used as primers for viral mRNAs. The PB2 subunit is responsible for the binding of the 5' cap of cellular pre-mRNAs which are subsequently cleaved after 10-13 nucleotides by the PA subunit that carries the endonuclease activity.UniRule annotation
Cofactori
Mn2+UniRule annotationNote: Binds 2 manganese ions per subunit.UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 41 | Manganese 1; via tele nitrogenUniRule annotation | 1 | |
Metal bindingi | 80 | Manganese 2UniRule annotation | 1 | |
Metal bindingi | 108 | Manganese 1UniRule annotation | 1 | |
Metal bindingi | 108 | Manganese 2UniRule annotation | 1 | |
Metal bindingi | 119 | Manganese 1UniRule annotation | 1 | |
Metal bindingi | 120 | Manganese 1; via carbonyl oxygenUniRule annotation | 1 |
GO - Molecular functioni
- endonuclease activity Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- RNA binding Source: UniProtKB-UniRule
GO - Biological processi
- cap snatching Source: UniProtKB-UniRule
- negative stranded viral RNA replication Source: UniProtKB
- suppression by virus of host RNA polymerase II activity Source: UniProtKB-UniRule
- transcription, DNA-templated Source: UniProtKB-UniRule
Keywordsi
Protein family/group databases
MEROPSi | S62.001 |
Names & Taxonomyi
Protein namesi | Recommended name: Polymerase acidic proteinUniRule annotation (EC:3.1.-.-UniRule annotation)Alternative name(s): RNA-directed RNA polymerase subunit P2UniRule annotation |
Gene namesi | Name:PAUniRule annotation |
Organismi | Influenza A virus (strain A/Wilson-Smith/1933 H1N1) (Influenza A virus (strain A/WS/1933 H1N1)) |
Taxonomic identifieri | 381518 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Negarnaviricota › Polyploviricotina › Insthoviricetes › Articulavirales › Orthomyxoviridae › Alphainfluenzavirus › |
Virus hosti | Aves [TaxID: 8782] Homo sapiens (Human) [TaxID: 9606] Sus scrofa (Pig) [TaxID: 9823] |
Proteomesi |
|
Subcellular locationi
- Host cytoplasm UniRule annotation
- Host nucleus UniRule annotation Note: PB1 and PA are transported in the host nucleus as a complex.UniRule annotationBy similarity
Keywords - Cellular componenti
Host cytoplasm, Host nucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 226 | L → P: Confers temperature sensitivity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000078801 | 1 – 716 | Polymerase acidic proteinAdd BLAST | 716 |
Post-translational modificationi
Phosphorylated on serines and threonines by host kinases, including human casein kinase II.UniRule annotationBy similarity
Keywords - PTMi
PhosphoproteinProteomic databases
PRIDEi | P15659 |
Interactioni
Subunit structurei
Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA.
Interacts (via C-terminus) with PB1 (via N-terminus).
UniRule annotationBy similarityBinary interactionsi
Hide detailsP15659
With | #Exp. | IntAct |
---|---|---|
PB2 [P03427] | 3 | EBI-8431752,EBI-8430745 |
USP11 [P51784] from Homo sapiens. | 2 | EBI-8431752,EBI-306876 |
Protein-protein interaction databases
IntActi | P15659, 32 interactors |
MINTi | P15659 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P15659 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 124 – 139 | Nuclear localization signal 1 (NLS1)UniRule annotationBy similarityAdd BLAST | 16 | |
Motifi | 184 – 247 | Nuclear localization signal 2 (NLS2)UniRule annotationBy similarityAdd BLAST | 64 |
Sequence similaritiesi
Belongs to the influenza viruses PA family.UniRule annotation
Family and domain databases
Gene3Di | 3.40.91.90, 1 hit |
HAMAPi | MF_04063, INFV_PA, 1 hit |
InterProi | View protein in InterPro IPR037534, INFV_PA IPR001009, PA/PA-X IPR038372, PA/PA-X_sf |
Pfami | View protein in Pfam PF00603, Flu_PA, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsiribosomal frameshifting. AlignAdd to basketIsoform PA (identifier: P15659-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEDFVRQCFN PMIVELAEKA MKEYGEDLKI ETNKFAAICT HLEVCFMYSD
60 70 80 90 100
FHFIDEQGES IVVELGDPNA LLKHRFEIIE GRDRTIAWTV INSICNTTGA
110 120 130 140 150
EKPKFLPDLY DYKKNRFIEI GVTRREVHIY YLEKANKIKS EKTHIHIFSF
160 170 180 190 200
TGEEMATKAD YTLDEESRAR IKTRLFTIRQ EMASRGLWDS FRQSERGEET
210 220 230 240 250
IEERFEITGT MRKLADQSLP PNFSSLENFR AYVDGFEPNG YIEGKLSQMS
260 270 280 290 300
KEVNARIEPF LKSTPRPLRL PDGPPCSQRS KFLLMDALKL SIEDPSHEGE
310 320 330 340 350
GIPLYDAIKC MRTFFGWKEP NVVKPHEKGI NPNYLLSWKQ VLAELQDIEN
360 370 380 390 400
EEKIPRTKNM KKTSQLKWAL GENMAPEKVD FDDCKDVGDL KQYDSDEPEL
410 420 430 440 450
RSLASWIQNE FNKACELTDS SWIELDEIGE DAAPIEHIAS MRRNYFTAEV
460 470 480 490 500
SHCRATEYIM KGVYINTALL NASCAAMDDF QLIPMISKCR TKEGRRKTNL
510 520 530 540 550
YGFIIKGRSH LRNDTDVVNF VSMEFSLTDP RLEPHKWEKY CVLEVGDMLL
560 570 580 590 600
RSAIGHVSRP MFLYVRTNGT SKIKMKWGME MRRCLLQSLQ QIESMIEAES
610 620 630 640 650
SVKEKDMTKE FFENKSETWP VGESPKGVEE GSIGKVCRTL LAKSVFNSLY
660 670 680 690 700
ASPQLEGFSA ESRKLLLIVQ ALRDNLEPGT FDLGGLYEAI EECLINDPWV
710
LLNASWFNSF LTHALR
Isoform PA-X (identifier: P0DJW8-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry P0DJW8.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X17336 Genomic RNA Translation: CAA35212.1 |
Keywords - Coding sequence diversityi
Ribosomal frameshiftingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X17336 Genomic RNA Translation: CAA35212.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4IUJ | X-ray | 1.90 | A | 254-716 | [»] | |
5IEQ | X-ray | 2.20 | A | 254-716 | [»] | |
5IF2 | X-ray | 2.35 | A | 254-716 | [»] | |
5IF5 | X-ray | 2.25 | A | 254-716 | [»] | |
5IF7 | X-ray | 2.65 | A | 254-716 | [»] | |
5IF8 | X-ray | 2.30 | A | 254-716 | [»] | |
5IFB | X-ray | 2.45 | A | 254-716 | [»] | |
5IFC | X-ray | 2.65 | A | 254-716 | [»] | |
5IFD | X-ray | 2.65 | A | 254-716 | [»] | |
5V0U | X-ray | 2.45 | A | 254-716 | [»] | |
6CFP | X-ray | 2.45 | A | 254-716 | [»] | |
SMRi | P15659 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | P15659, 32 interactors |
MINTi | P15659 |
Protein family/group databases
MEROPSi | S62.001 |
Proteomic databases
PRIDEi | P15659 |
Family and domain databases
Gene3Di | 3.40.91.90, 1 hit |
HAMAPi | MF_04063, INFV_PA, 1 hit |
InterProi | View protein in InterPro IPR037534, INFV_PA IPR001009, PA/PA-X IPR038372, PA/PA-X_sf |
Pfami | View protein in Pfam PF00603, Flu_PA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PA_I33A0 | |
Accessioni | P15659Primary (citable) accession number: P15659 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1990 |
Last sequence update: | April 1, 1990 | |
Last modified: | April 7, 2021 | |
This is version 107 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families