UniProtKB - P15640 (PUR2_ECOLI)
Protein
Phosphoribosylamine--glycine ligase
Gene
purD
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalytic activityi
- 5-phospho-β-D-ribosylamine + ATP + glycine = ADP + H+ + N1-(5-phospho-β-D-ribosyl)glycinamide + phosphateEC:6.3.4.13
Cofactori
: IMP biosynthesis via de novo pathway Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.Proteins known to be involved in the 2 steps of the subpathway in this organism are:
- Amidophosphoribosyltransferase (purF), Amidophosphoribosyltransferase (purF)
- Phosphoribosylamine--glycine ligase (purD), Phosphoribosylamine--glycine ligase (purD)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 286 | Magnesium or manganeseBy similarity | 1 | |
Metal bindingi | 288 | Magnesium or manganeseBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 135 – 196 | ATPBy similarityAdd BLAST | 62 |
GO - Molecular functioni
- ATPase activity Source: EcoliWiki
- ATP binding Source: EcoliWiki
- metal ion binding Source: UniProtKB-KW
- phosphoribosylamine-glycine ligase activity Source: EcoCyc
GO - Biological processi
- 'de novo' IMP biosynthetic process Source: UniProtKB-UniRule
- cellular response to DNA damage stimulus Source: EcoliWiki
- purine nucleobase biosynthetic process Source: InterPro
- purine nucleotide biosynthetic process Source: EcoliWiki
Keywordsi
Molecular function | Ligase |
Biological process | Purine biosynthesis |
Ligand | ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:GLYCRIBONUCSYN-MONOMER MetaCyc:GLYCRIBONUCSYN-MONOMER |
BRENDAi | 6.3.4.13, 2026 |
UniPathwayi | UPA00074;UER00125 |
Names & Taxonomyi
Protein namesi | Recommended name: Phosphoribosylamine--glycine ligase (EC:6.3.4.13)Alternative name(s): GARS Glycinamide ribonucleotide synthetase Phosphoribosylglycinamide synthetase |
Gene namesi | Name:purD Ordered Locus Names:b4005, JW3969 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000151448 | 1 – 429 | Phosphoribosylamine--glycine ligaseAdd BLAST | 429 |
Proteomic databases
jPOSTi | P15640 |
PaxDbi | P15640 |
PRIDEi | P15640 |
2D gel databases
SWISS-2DPAGEi | P15640 |
Interactioni
Subunit structurei
Monomer.
Protein-protein interaction databases
BioGRIDi | 4262462, 50 interactors |
IntActi | P15640, 4 interactors |
STRINGi | 511145.b4005 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P15640 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P15640 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 109 – 316 | ATP-graspAdd BLAST | 208 |
Sequence similaritiesi
Belongs to the GARS family.Curated
Phylogenomic databases
eggNOGi | COG0151, Bacteria |
HOGENOMi | CLU_027420_3_1_6 |
InParanoidi | P15640 |
PhylomeDBi | P15640 |
Family and domain databases
Gene3Di | 3.30.1490.20, 1 hit 3.90.600.10, 1 hit |
HAMAPi | MF_00138, GARS, 1 hit |
InterProi | View protein in InterPro IPR011761, ATP-grasp IPR013815, ATP_grasp_subdomain_1 IPR016185, PreATP-grasp_dom_sf IPR020561, PRibGlycinamid_synth_ATP-grasp IPR000115, PRibGlycinamide_synth IPR020560, PRibGlycinamide_synth_C-dom IPR037123, PRibGlycinamide_synth_C_sf IPR020559, PRibGlycinamide_synth_CS IPR020562, PRibGlycinamide_synth_N IPR011054, Rudment_hybrid_motif |
PANTHERi | PTHR43472, PTHR43472, 1 hit |
Pfami | View protein in Pfam PF01071, GARS_A, 1 hit PF02843, GARS_C, 1 hit PF02844, GARS_N, 1 hit |
SMARTi | View protein in SMART SM01210, GARS_C, 1 hit |
SUPFAMi | SSF51246, SSF51246, 1 hit SSF52440, SSF52440, 1 hit |
TIGRFAMsi | TIGR00877, purD, 1 hit |
PROSITEi | View protein in PROSITE PS50975, ATP_GRASP, 1 hit PS00184, GARS, 1 hit |
i Sequence
Sequence statusi: Complete.
P15640-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKVLVIGNGG REHALAWKAA QSPLVETVFV APGNAGTALE PALQNVAIGV
60 70 80 90 100
TDIPALLDFA QNEKIDLTIV GPEAPLVKGV VDTFRAAGLK IFGPTAGAAQ
110 120 130 140 150
LEGSKAFTKD FLARHKIPTA EYQNFTEVEP ALAYLREKGA PIVIKADGLA
160 170 180 190 200
AGKGVIVAMT LEEAEAAVHD MLAGNAFGDA GHRIVIEEFL DGEEASFIVM
210 220 230 240 250
VDGEHVLPMA TSQDHKRVGD KDTGPNTGGM GAYSPAPVVT DDVHQRTMER
260 270 280 290 300
IIWPTVKGMA AEGNTYTGFL YAGLMIDKQG NPKVIEFNCR FGDPETQPIM
310 320 330 340 350
LRMKSDLVEL CLAACESKLD EKTSEWDERA SLGVVMAAGG YPGDYRTGDV
360 370 380 390 400
IHGLPLEEVA GGKVFHAGTK LADDEQVVTN GGRVLCVTAL GHTVAEAQKR
410 420
AYALMTDIHW DDCFCRKDIG WRAIEREQN
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 15 – 16 | LA → WR in AAA24455 (PubMed:2687276).Curated | 2 | |
Sequence conflicti | 97 | G → V in AAA24455 (PubMed:2687276).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J05126 Genomic DNA Translation: AAA24455.1 X51950 Genomic DNA Translation: CAA36213.1 U00006 Genomic DNA Translation: AAC43103.1 U00096 Genomic DNA Translation: AAC76979.1 AP009048 Genomic DNA Translation: BAE77314.1 |
PIRi | A33771, AJECQG |
RefSeqi | NP_418433.1, NC_000913.3 WP_000866800.1, NZ_SSZK01000047.1 |
Genome annotation databases
EnsemblBacteriai | AAC76979; AAC76979; b4005 BAE77314; BAE77314; BAE77314 |
GeneIDi | 948504 |
KEGGi | ecj:JW3969 eco:b4005 |
PATRICi | fig|1411691.4.peg.2705 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J05126 Genomic DNA Translation: AAA24455.1 X51950 Genomic DNA Translation: CAA36213.1 U00006 Genomic DNA Translation: AAC43103.1 U00096 Genomic DNA Translation: AAC76979.1 AP009048 Genomic DNA Translation: BAE77314.1 |
PIRi | A33771, AJECQG |
RefSeqi | NP_418433.1, NC_000913.3 WP_000866800.1, NZ_SSZK01000047.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1GSO | X-ray | 1.60 | A | 1-429 | [»] | |
SMRi | P15640 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262462, 50 interactors |
IntActi | P15640, 4 interactors |
STRINGi | 511145.b4005 |
2D gel databases
SWISS-2DPAGEi | P15640 |
Proteomic databases
jPOSTi | P15640 |
PaxDbi | P15640 |
PRIDEi | P15640 |
Genome annotation databases
EnsemblBacteriai | AAC76979; AAC76979; b4005 BAE77314; BAE77314; BAE77314 |
GeneIDi | 948504 |
KEGGi | ecj:JW3969 eco:b4005 |
PATRICi | fig|1411691.4.peg.2705 |
Organism-specific databases
EchoBASEi | EB0785 |
Phylogenomic databases
eggNOGi | COG0151, Bacteria |
HOGENOMi | CLU_027420_3_1_6 |
InParanoidi | P15640 |
PhylomeDBi | P15640 |
Enzyme and pathway databases
UniPathwayi | UPA00074;UER00125 |
BioCyci | EcoCyc:GLYCRIBONUCSYN-MONOMER MetaCyc:GLYCRIBONUCSYN-MONOMER |
BRENDAi | 6.3.4.13, 2026 |
Miscellaneous databases
EvolutionaryTracei | P15640 |
PROi | PR:P15640 |
Family and domain databases
Gene3Di | 3.30.1490.20, 1 hit 3.90.600.10, 1 hit |
HAMAPi | MF_00138, GARS, 1 hit |
InterProi | View protein in InterPro IPR011761, ATP-grasp IPR013815, ATP_grasp_subdomain_1 IPR016185, PreATP-grasp_dom_sf IPR020561, PRibGlycinamid_synth_ATP-grasp IPR000115, PRibGlycinamide_synth IPR020560, PRibGlycinamide_synth_C-dom IPR037123, PRibGlycinamide_synth_C_sf IPR020559, PRibGlycinamide_synth_CS IPR020562, PRibGlycinamide_synth_N IPR011054, Rudment_hybrid_motif |
PANTHERi | PTHR43472, PTHR43472, 1 hit |
Pfami | View protein in Pfam PF01071, GARS_A, 1 hit PF02843, GARS_C, 1 hit PF02844, GARS_N, 1 hit |
SMARTi | View protein in SMART SM01210, GARS_C, 1 hit |
SUPFAMi | SSF51246, SSF51246, 1 hit SSF52440, SSF52440, 1 hit |
TIGRFAMsi | TIGR00877, purD, 1 hit |
PROSITEi | View protein in PROSITE PS50975, ATP_GRASP, 1 hit PS00184, GARS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PUR2_ECOLI | |
Accessioni | P15640Primary (citable) accession number: P15640 Secondary accession number(s): Q2M8U2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1990 |
Last sequence update: | October 1, 1993 | |
Last modified: | April 7, 2021 | |
This is version 171 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families