UniProtKB - P15505 (GCSP_CHICK)
Protein
Glycine dehydrogenase (decarboxylating), mitochondrial
Gene
GLDC
Organism
Gallus gallus (Chicken)
Status
Functioni
The glycine cleavage system catalyzes the degradation of glycine. The P protein (GLDC) binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO2 is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein (GCSH).3 Publications
Catalytic activityi
- glycine + H+ + N6-lipoyl-L-lysyl-[glycine-cleavage complex H protein] = (R)-N6-(S8-aminomethyldihydrolipoyl)-L-lysyl-[glycine-cleavage complex H protein] + CO23 PublicationsEC:1.4.4.23 Publications
Cofactori
pyridoxal 5'-phosphate1 Publication
Activity regulationi
Stimulated by lipoic acid. Inhibited in presence of methylamine.2 Publications
Kineticsi
- KM=20 mM for glycine in presence of GCSH1 Publication
- KM=27 mM for methylamine in presence of GCSH1 Publication
- KM=40 mM for glycine1 Publication
- KM=3.5 mM for lipoic acid1 Publication
- KM=63 mM for methylamine1 Publication
GO - Molecular functioni
- glycine dehydrogenase (decarboxylating) activity Source: UniProtKB
- glycine dehydrogenase activity Source: UniProtKB
- lyase activity Source: InterPro
- protein homodimerization activity Source: UniProtKB
- pyridoxal binding Source: UniProtKB
GO - Biological processi
- glycine catabolic process Source: UniProtKB
- response to lipoic acid Source: UniProtKB
- response to methylamine Source: UniProtKB
Keywordsi
Molecular function | Oxidoreductase |
Ligand | Pyridoxal phosphate |
Enzyme and pathway databases
BioCyci | MetaCyc:MONOMER-12926 |
BRENDAi | 1.4.4.2, 1306 |
Names & Taxonomyi
Protein namesi | Recommended name: Glycine dehydrogenase (decarboxylating), mitochondrialCurated (EC:1.4.4.23 Publications)Alternative name(s): Glycine cleavage system P protein Glycine decarboxylase1 Publication Glycine dehydrogenase (aminomethyl-transferring) |
Gene namesi | Name:GLDCBy similarity |
Organismi | Gallus gallus (Chicken) |
Taxonomic identifieri | 9031 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archelosauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galloanserae › Galliformes › Phasianidae › Phasianinae › Gallus |
Proteomesi |
|
Subcellular locationi
Mitochondrion
- Mitochondrion 1 Publication
Mitochondrion
- mitochondrion Source: UniProtKB
Keywords - Cellular componenti
MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – ? | MitochondrionSequence analysis | ||
ChainiPRO_0000010742 | ? – 1004 | Glycine dehydrogenase (decarboxylating), mitochondrial |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 738 | N6-(pyridoxal phosphate)lysine1 Publication | 1 |
Proteomic databases
PaxDbi | P15505 |
PRIDEi | P15505 |
Expressioni
Tissue specificityi
Liver (at protein level).1 Publication
Interactioni
Subunit structurei
Homodimer.
Interacts with GCSH. The glycine cleavage system is composed of four proteins: P (GLDC), T (GCST), L (DLD) and H (GCSH).
2 PublicationsGO - Molecular functioni
- protein homodimerization activity Source: UniProtKB
Protein-protein interaction databases
STRINGi | 9031.ENSGALP00000037556 |
Family & Domainsi
Sequence similaritiesi
Belongs to the GcvP family.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG2040, Eukaryota |
InParanoidi | P15505 |
PhylomeDBi | P15505 |
Family and domain databases
CDDi | cd00613, GDC-P, 2 hits |
Gene3Di | 3.40.640.10, 2 hits 3.90.1150.10, 1 hit |
HAMAPi | MF_00711, GcvP, 1 hit |
InterProi | View protein in InterPro IPR001597, ArAA_b-elim_lyase/Thr_aldolase IPR003437, GcvP IPR020581, GDC_P IPR015424, PyrdxlP-dep_Trfase IPR015422, PyrdxlP-dep_Trfase_dom1 IPR015421, PyrdxlP-dep_Trfase_major |
PANTHERi | PTHR11773, PTHR11773, 1 hit |
Pfami | View protein in Pfam PF01212, Beta_elim_lyase, 1 hit PF02347, GDC-P, 1 hit |
SUPFAMi | SSF53383, SSF53383, 2 hits |
TIGRFAMsi | TIGR00461, gcvP, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P15505-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQSCGRWWGR LAARGAPRHL RPAAGGPRRQ QQRWGGGEAA RCIEQLLPRH
60 70 80 90 100
DDFCRRHIGP REREKREMLS AVGVQSVEEL MDKTIPASIR LRRPLRMDDH
110 120 130 140 150
VVENEILETL YNIASKNKIW RSYIGMGYYN CSVPQPIARN LLENAGWVTQ
160 170 180 190 200
YTPYQPEVSQ GRLESLLNYQ TMVCDITGMD VANASLLDEG TAAAEAMQLC
210 220 230 240 250
HRQNKRRKFY IDARCHPQTI ANYTGVITEL KLPHEMDFSG KDVSGVLFQY
260 270 280 290 300
PDTEGKVEDF SELIERAHQN GTLACCATDL LALCILKPPG EFGVDVVLGS
310 320 330 340 350
SQRFGVPLCY GGPHAAFFAV KENLVRMMPG RMVGVTRDAN GKEVYRLALQ
360 370 380 390 400
TREQHIRRDK ATSNICTAQA LLANMAAMYG VYHGSDGLKD IARRVHNATL
410 420 430 440 450
ILAEGLRRAG HKLHHDLFFD TLTVTCGCSV KEVLDRAALR KINVRIYSDG
460 470 480 490 500
RLGVSLDETV NEKDLDDILW IFGCESSAEL VAEGMGEETK GILSTPFKRT
510 520 530 540 550
SKFLTHQVFN SYHSETNIVR YMKRLENKDI SLVHSMIPLG SCTMKLNSSA
560 570 580 590 600
ELAPISWKEF ANIHPFVPLD QAQGYQQLFK DLEKDLCEIT GYDKISFQPN
610 620 630 640 650
SGAQGEYAGL AAIKAYLNAK GERHRSVCLI PRSAHGTNPA SAQMAGMKIQ
660 670 680 690 700
PIEVDKNGSI DISHLKAMVD KHKENLAAIM ITYPSTNGVF EEEIGDVCDL
710 720 730 740 750
IHKHGGQVYL DGANMNAQVG LCRPGDYGSD VSHLNLHKTF CIPHGGGGPG
760 770 780 790 800
MGPIGVKKHL APYLPTHPVI KIQTDKDACP LGTVSAAPWG SSAILPISWV
810 820 830 840 850
YIKTMGAKGL KHASEIAILN ANYMAKRLEK HYKILFRGVR GYVAHEFILD
860 870 880 890 900
TRPFKKTANI EAVDLAKRLQ DYGFHAPTMS WPVAGTLMIE PTESEDKGEL
910 920 930 940 950
DRFCDAMISI RQEIADIEEG RMDPQVNPLK MSPHTLNCVT SSKWDRPYSR
960 970 980 990 1000
EVAAFPLPFV KPESKFWPTI ARIDDIYGDQ HLVCTCPPME AYESPFSEQK
RASS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M64402 mRNA Translation: AAA49029.1 D90266 mRNA Translation: BAA14313.1 D90240 Genomic DNA Translation: BAA14287.1 |
PIRi | A39521 |
RefSeqi | NP_989653.1, NM_204322.1 |
Genome annotation databases
GeneIDi | 374222 |
KEGGi | gga:374222 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M64402 mRNA Translation: AAA49029.1 D90266 mRNA Translation: BAA14313.1 D90240 Genomic DNA Translation: BAA14287.1 |
PIRi | A39521 |
RefSeqi | NP_989653.1, NM_204322.1 |
3D structure databases
SMRi | P15505 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9031.ENSGALP00000037556 |
Proteomic databases
PaxDbi | P15505 |
PRIDEi | P15505 |
Genome annotation databases
GeneIDi | 374222 |
KEGGi | gga:374222 |
Organism-specific databases
CTDi | 2731 |
Phylogenomic databases
eggNOGi | KOG2040, Eukaryota |
InParanoidi | P15505 |
PhylomeDBi | P15505 |
Enzyme and pathway databases
BioCyci | MetaCyc:MONOMER-12926 |
BRENDAi | 1.4.4.2, 1306 |
Miscellaneous databases
PROi | PR:P15505 |
Family and domain databases
CDDi | cd00613, GDC-P, 2 hits |
Gene3Di | 3.40.640.10, 2 hits 3.90.1150.10, 1 hit |
HAMAPi | MF_00711, GcvP, 1 hit |
InterProi | View protein in InterPro IPR001597, ArAA_b-elim_lyase/Thr_aldolase IPR003437, GcvP IPR020581, GDC_P IPR015424, PyrdxlP-dep_Trfase IPR015422, PyrdxlP-dep_Trfase_dom1 IPR015421, PyrdxlP-dep_Trfase_major |
PANTHERi | PTHR11773, PTHR11773, 1 hit |
Pfami | View protein in Pfam PF01212, Beta_elim_lyase, 1 hit PF02347, GDC-P, 1 hit |
SUPFAMi | SSF53383, SSF53383, 2 hits |
TIGRFAMsi | TIGR00461, gcvP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GCSP_CHICK | |
Accessioni | P15505Primary (citable) accession number: P15505 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1990 |
Last sequence update: | May 1, 1992 | |
Last modified: | December 2, 2020 | |
This is version 126 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - UniProtKB entry view manual
User manual for the UniProtKB entry view