UniProtKB - P15429 (ENOB_RAT)
Protein
Beta-enolase
Gene
Eno3
Organism
Rattus norvegicus (Rat)
Status
Functioni
Appears to have a function in striated muscle development and regeneration.
Catalytic activityi
- EC:4.2.1.11
Cofactori
Mg2+Note: Mg2+ is required for catalysis and for stabilizing the dimer.
: glycolysis Pathwayi
This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.Proteins known to be involved in the 5 steps of the subpathway in this organism are:
- Glyceraldehyde-3-phosphate dehydrogenase (Gapdh), Glyceraldehyde-3-phosphate dehydrogenase (LOC108351137), Glyceraldehyde-3-phosphate dehydrogenase, Glyceraldehyde-3-phosphate dehydrogenase, Glyceraldehyde-3-phosphate dehydrogenase, Glyceraldehyde-3-phosphate dehydrogenase, testis-specific (Gapdhs), Glyceraldehyde-3-phosphate dehydrogenase, Glyceraldehyde-3-phosphate dehydrogenase (Gapdhs), Glyceraldehyde-3-phosphate dehydrogenase, Glyceraldehyde-3-phosphate dehydrogenase
- Phosphoglycerate kinase 1 (Pgk1), Phosphoglycerate kinase (RGD1560402), Phosphoglycerate kinase (Pgk1), Phosphoglycerate kinase (Pgk2)
- no protein annotated in this organism
- Alpha-enolase (Eno1), 2-phospho-D-glycerate hydro-lyase (Eno1), 2-phospho-D-glycerate hydro-lyase (Eno1), 2-phospho-D-glycerate hydro-lyase, 2-phospho-D-glycerate hydro-lyase (Eno4), Beta-enolase (Eno3), Gamma-enolase (Eno2), 2-phospho-D-glycerate hydro-lyase, 2-phospho-D-glycerate hydro-lyase
- Pyruvate kinase PKLR (Pklr), Pyruvate kinase (Pklr), Pyruvate kinase (LOC100362738), Pyruvate kinase PKM (Pkm), Pyruvate kinase (Pkm), Pyruvate kinase (Pklr), Pyruvate kinase
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 158 | SubstrateBy similarity | 1 | |
Binding sitei | 167 | SubstrateBy similarity | 1 | |
Active sitei | 210 | Proton donorBy similarity | 1 | |
Metal bindingi | 245 | MagnesiumBy similarity | 1 | |
Metal bindingi | 293 | MagnesiumBy similarity | 1 | |
Binding sitei | 293 | SubstrateBy similarity | 1 | |
Metal bindingi | 318 | MagnesiumBy similarity | 1 | |
Binding sitei | 318 | SubstrateBy similarity | 1 | |
Active sitei | 343 | Proton acceptorBy similarity | 1 | |
Binding sitei | 394 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- identical protein binding Source: RGD
- magnesium ion binding Source: InterPro
- phosphopyruvate hydratase activity Source: RGD
- protein-containing complex binding Source: RGD
GO - Biological processi
- aging Source: RGD
- glycolytic process Source: GO_Central
- regulation of vacuole fusion, non-autophagic Source: GO_Central
- response to drug Source: RGD
- skeletal muscle tissue regeneration Source: RGD
Keywordsi
Molecular function | Lyase |
Biological process | Glycolysis |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
Reactomei | R-RNO-70171, Glycolysis R-RNO-70263, Gluconeogenesis |
UniPathwayi | UPA00109;UER00187 |
Names & Taxonomyi
Protein namesi | Recommended name: Beta-enolase (EC:4.2.1.11)Alternative name(s): 2-phospho-D-glycerate hydro-lyase Enolase 3 Muscle-specific enolase Short name: MSE Skeletal muscle enolase |
Gene namesi | Name:Eno3 Synonyms:Eno-3 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2555, Eno3 |
Subcellular locationi
Other locations
Note: Localized to the Z line. Some colocalization with CKM at M-band (By similarity).By similarity
Cytosol
- phosphopyruvate hydratase complex Source: GO_Central
Plasma Membrane
- plasma membrane Source: Ensembl
Other locations
- membrane Source: RGD
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000134110 | 2 – 434 | Beta-enolaseAdd BLAST | 433 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 | |
Modified residuei | 72 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 83 | PhosphoserineCombined sources | 1 | |
Modified residuei | 157 | PhosphoserineCombined sources | 1 | |
Modified residuei | 176 | PhosphoserineBy similarity | 1 | |
Modified residuei | 205 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 229 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 236 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 263 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
jPOSTi | P15429 |
PaxDbi | P15429 |
PRIDEi | P15429 |
PTM databases
iPTMneti | P15429 |
PhosphoSitePlusi | P15429 |
SwissPalmi | P15429 |
Expressioni
Tissue specificityi
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
Developmental stagei
During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells.1 Publication
Inductioni
Thyroid hormones up-regulate expression during hindleg muscle development and down-regulate during cardiac muscle development. Decrease in ENO3 levels with aortic stenosis.
Gene expression databases
Bgeei | ENSRNOG00000004078, Expressed in skeletal muscle tissue and 21 other tissues |
Genevisiblei | P15429, RN |
Interactioni
Subunit structurei
Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers which are cell-type and development-specific.
Interacts with PNKD (By similarity).
By similarityBinary interactionsi
P15429
With | #Exp. | IntAct |
---|---|---|
PDE4DIP - isoform 11 [Q5VU43-11] from Homo sapiens. | 2 | EBI-10817548,EBI-10769071 |
GO - Molecular functioni
- identical protein binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 247473, 1 interactor |
IntActi | P15429, 1 interactor |
STRINGi | 10116.ENSRNOP00000005612 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 370 – 373 | Substrate bindingBy similarity | 4 |
Sequence similaritiesi
Belongs to the enolase family.Curated
Phylogenomic databases
eggNOGi | KOG2670, Eukaryota |
GeneTreei | ENSGT00950000182805 |
HOGENOMi | CLU_031223_0_0_1 |
InParanoidi | P15429 |
OMAi | QEFLVVP |
OrthoDBi | 773373at2759 |
PhylomeDBi | P15429 |
TreeFami | TF300391 |
Family and domain databases
CDDi | cd03313, enolase, 1 hit |
Gene3Di | 3.20.20.120, 1 hit 3.30.390.10, 1 hit |
HAMAPi | MF_00318, Enolase, 1 hit |
InterProi | View protein in InterPro IPR000941, Enolase IPR036849, Enolase-like_C_sf IPR029017, Enolase-like_N IPR020810, Enolase_C IPR020809, Enolase_CS IPR020811, Enolase_N |
PANTHERi | PTHR11902, PTHR11902, 1 hit |
Pfami | View protein in Pfam PF00113, Enolase_C, 1 hit PF03952, Enolase_N, 1 hit |
PIRSFi | PIRSF001400, Enolase, 1 hit |
PRINTSi | PR00148, ENOLASE |
SMARTi | View protein in SMART SM01192, Enolase_C, 1 hit SM01193, Enolase_N, 1 hit |
SUPFAMi | SSF51604, SSF51604, 1 hit SSF54826, SSF54826, 1 hit |
TIGRFAMsi | TIGR01060, eno, 1 hit |
PROSITEi | View protein in PROSITE PS00164, ENOLASE, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P15429-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAMQKIFARE ILDSRGNPTV EVDLHTAKGR FRAAVPSGAS TGIYEALELR
60 70 80 90 100
DGDKSRYLGK GVLKAVEHIN KTLGPALLEK KLSVVDQEKV DKFMIELDGT
110 120 130 140 150
ENKSKFGANA ILGVSLAVCK AGAAEKGVPL YRHIADLAGN PDLVLPVPAF
160 170 180 190 200
NVINGGSHAG NKLAMQEFMI LPVGASSFKE AMRIGAEVYH HLKGVIKAKY
210 220 230 240 250
GKDATNVGDE GGFAPNILEN NEALELLKTA IQAAGYPDKV VIGMDVAASE
260 270 280 290 300
FYRNGKYDLD FKSPDDPARH ISGEKLGELY KSFIKNYPVV SIEDPFDQDD
310 320 330 340 350
WATWTSFLSG VDIQIVGDDL TVTNPKRIAQ AVEKKACNCL LLKVNQIGSV
360 370 380 390 400
TESIQACKLA QSNGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR
410 420 430
SERLAKYNQL MRIEEALGDK AVFAGRKFRN PKAK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 63 | L → P in CAA68788 (PubMed:2914621).Curated | 1 | |
Sequence conflicti | 176 – 182 | SSFKEAM → KLFQGSQ in CAA68788 (PubMed:2914621).Curated | 7 | |
Sequence conflicti | 279 | L → P in CAA68788 (PubMed:2914621).Curated | 1 | |
Sequence conflicti | 355 | Q → L in CAA68788 (PubMed:2914621).Curated | 1 | |
Sequence conflicti | 381 | I → V in CAA68788 (PubMed:2914621).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y00979 mRNA Translation: CAA68788.1 BC083566 mRNA Translation: AAH83566.1 X57774 Genomic DNA Translation: CAA40920.1 |
PIRi | S02072 |
RefSeqi | NP_037081.2, NM_012949.2 XP_006246661.1, XM_006246599.3 XP_006246662.1, XM_006246600.3 |
Genome annotation databases
Ensembli | ENSRNOT00000005612; ENSRNOP00000005612; ENSRNOG00000004078 |
GeneIDi | 25438 |
KEGGi | rno:25438 |
UCSCi | RGD:2555, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y00979 mRNA Translation: CAA68788.1 BC083566 mRNA Translation: AAH83566.1 X57774 Genomic DNA Translation: CAA40920.1 |
PIRi | S02072 |
RefSeqi | NP_037081.2, NM_012949.2 XP_006246661.1, XM_006246599.3 XP_006246662.1, XM_006246600.3 |
3D structure databases
SMRi | P15429 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 247473, 1 interactor |
IntActi | P15429, 1 interactor |
STRINGi | 10116.ENSRNOP00000005612 |
PTM databases
iPTMneti | P15429 |
PhosphoSitePlusi | P15429 |
SwissPalmi | P15429 |
Proteomic databases
jPOSTi | P15429 |
PaxDbi | P15429 |
PRIDEi | P15429 |
Genome annotation databases
Ensembli | ENSRNOT00000005612; ENSRNOP00000005612; ENSRNOG00000004078 |
GeneIDi | 25438 |
KEGGi | rno:25438 |
UCSCi | RGD:2555, rat |
Organism-specific databases
CTDi | 2027 |
RGDi | 2555, Eno3 |
Phylogenomic databases
eggNOGi | KOG2670, Eukaryota |
GeneTreei | ENSGT00950000182805 |
HOGENOMi | CLU_031223_0_0_1 |
InParanoidi | P15429 |
OMAi | QEFLVVP |
OrthoDBi | 773373at2759 |
PhylomeDBi | P15429 |
TreeFami | TF300391 |
Enzyme and pathway databases
UniPathwayi | UPA00109;UER00187 |
Reactomei | R-RNO-70171, Glycolysis R-RNO-70263, Gluconeogenesis |
Miscellaneous databases
PROi | PR:P15429 |
Gene expression databases
Bgeei | ENSRNOG00000004078, Expressed in skeletal muscle tissue and 21 other tissues |
Genevisiblei | P15429, RN |
Family and domain databases
CDDi | cd03313, enolase, 1 hit |
Gene3Di | 3.20.20.120, 1 hit 3.30.390.10, 1 hit |
HAMAPi | MF_00318, Enolase, 1 hit |
InterProi | View protein in InterPro IPR000941, Enolase IPR036849, Enolase-like_C_sf IPR029017, Enolase-like_N IPR020810, Enolase_C IPR020809, Enolase_CS IPR020811, Enolase_N |
PANTHERi | PTHR11902, PTHR11902, 1 hit |
Pfami | View protein in Pfam PF00113, Enolase_C, 1 hit PF03952, Enolase_N, 1 hit |
PIRSFi | PIRSF001400, Enolase, 1 hit |
PRINTSi | PR00148, ENOLASE |
SMARTi | View protein in SMART SM01192, Enolase_C, 1 hit SM01193, Enolase_N, 1 hit |
SUPFAMi | SSF51604, SSF51604, 1 hit SSF54826, SSF54826, 1 hit |
TIGRFAMsi | TIGR01060, eno, 1 hit |
PROSITEi | View protein in PROSITE PS00164, ENOLASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ENOB_RAT | |
Accessioni | P15429Primary (citable) accession number: P15429 Secondary accession number(s): Q5XIV3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1990 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 173 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families