UniProtKB - P15423 (SPIKE_CVH22)
Spike glycoprotein
S
Functioni
S1 region attaches the virion to the cell membrane by interacting with host ANPEP/aminopeptidase N, initiating the infection. Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide of S2 region and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.
UniRule annotation4 PublicationsCaution
GO - Biological processi
- endocytosis involved in viral entry into host cell Source: UniProtKB-UniRule
- fusion of virus membrane with host endosome membrane Source: UniProtKB-UniRule
- fusion of virus membrane with host plasma membrane Source: UniProtKB-UniRule
- receptor-mediated virion attachment to host cell Source: UniProtKB-UniRule
Keywordsi
Biological process | Host-virus interaction, Viral attachment to host cell, Virulence, Virus entry into host cell |
Enzyme and pathway databases
SABIO-RKi | P15423 |
Names & Taxonomyi
Protein namesi | Recommended name: Spike glycoproteinUniRule annotationShort name: S glycoproteinUniRule annotation Alternative name(s): E2UniRule annotation Peplomer proteinUniRule annotation |
Gene namesi | Name:SUniRule annotation ORF Names:2 |
Organismi | Human coronavirus 229E (HCoV-229E) |
Taxonomic identifieri | 11137 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Pisuviricota › Pisoniviricetes › Nidovirales › Cornidovirineae › Coronaviridae › Orthocoronavirinae › Alphacoronavirus › Duvinacovirus |
Virus hosti | Homo sapiens (Human) [TaxID: 9606] |
Proteomesi |
|
Subcellular locationi
- Virion membrane UniRule annotation; Single-pass type I membrane protein UniRule annotation
- Host endoplasmic reticulum-Golgi intermediate compartment membrane UniRule annotation; Single-pass type I membrane protein UniRule annotation Note: Accumulates in the endoplasmic reticulum-Golgi intermediate compartment, where it participates in virus particle assembly.UniRule annotation
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 16 – 1115 | Virion surfaceUniRule annotationAdd BLAST | 1100 | |
Transmembranei | 1116 – 1135 | HelicalUniRule annotationAdd BLAST | 20 | |
Topological domaini | 1136 – 1173 | IntravirionUniRule annotationAdd BLAST | 38 |
Keywords - Cellular componenti
Host membrane, Membrane, Viral envelope protein, VirionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 15 | UniRule annotationAdd BLAST | 15 | |
ChainiPRO_0000037203 | 16 – 1173 | Spike glycoproteinUniRule annotationAdd BLAST | 1158 |
Keywords - PTMi
GlycoproteinProteomic databases
PRIDEi | P15423 |
Interactioni
Subunit structurei
Homotrimer. During virus morphogenesis, found in a complex with M and HE proteins.
Interacts with host ANPEP.
UniRule annotation4 PublicationsStructurei
Secondary structure
3D structure databases
SMRi | P15423 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 16 – 536 | S1UniRule annotationAdd BLAST | 521 | |
Regioni | 417 – 547 | Interaction with ANPEPAdd BLAST | 131 | |
Regioni | 417 – 547 | Interaction with host ANPEPUniRule annotationAdd BLAST | 131 | |
Regioni | 537 – 1173 | S2UniRule annotationAdd BLAST | 637 | |
Regioni | 753 – 773 | Fusion peptideUniRule annotationAdd BLAST | 21 | |
Regioni | 767 – 886 | Heptad repeat 1 (HR1)PROSITE-ProRule annotationAdd BLAST | 120 | |
Regioni | 1031 – 1127 | Heptad repeat 2 (HR2)PROSITE-ProRule annotationAdd BLAST | 97 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 834 – 878 | UniRule annotationAdd BLAST | 45 | |
Coiled coili | 1063 – 1105 | UniRule annotationAdd BLAST | 43 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 1169 – 1173 | KxHxxUniRule annotation | 5 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Coiled coil, Signal, Transmembrane, Transmembrane helixFamily and domain databases
HAMAPi | MF_04200, ALPHA_CORONA_SPIKE, 1 hit |
InterProi | View protein in InterPro IPR042552, ALPHA_CORONA_SPIKE IPR043607, CoV_S1_C IPR043473, S2_sf_CoV IPR002551, Spike_S1_CoV IPR002552, Spike_S2_CoV IPR043614, Spike_S2_CoV_C IPR044873, Spike_S2_CoV_HR1 IPR044874, Spike_S2_CoV_HR2 |
Pfami | View protein in Pfam PF01600, CoV_S1, 1 hit PF19209, CoV_S1_C, 1 hit PF01601, CoV_S2, 1 hit PF19214, CoV_S2_C, 1 hit |
SUPFAMi | SSF111474, SSF111474, 2 hits |
PROSITEi | View protein in PROSITE PS51923, COV_S2_HR1, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MFVLLVAYAL LHIAGCQTTN GLNTSYSVCN GCVGYSENVF AVESGGYIPS
60 70 80 90 100
DFAFNNWFLL TNTSSVVDGV VRSFQPLLLN CLWSVSGLRF TTGFVYFNGT
110 120 130 140 150
GRGDCKGFSS DVLSDVIRYN LNFEENLRRG TILFKTSYGV VVFYCTNNTL
160 170 180 190 200
VSGDAHIPFG TVLGNFYCFV NTTIGNETTS AFVGALPKTV REFVISRTGH
210 220 230 240 250
FYINGYRYFT LGNVEAVNFN VTTAETTDFC TVALASYADV LVNVSQTSIA
260 270 280 290 300
NIIYCNSVIN RLRCDQLSFD VPDGFYSTSP IQSVELPVSI VSLPVYHKHT
310 320 330 340 350
FIVLYVDFKP QSGGGKCFNC YPAGVNITLA NFNETKGPLC VDTSHFTTKY
360 370 380 390 400
VAVYANVGRW SASINTGNCP FSFGKVNNFV KFGSVCFSLK DIPGGCAMPI
410 420 430 440 450
VANWAYSKYY TIGSLYVSWS DGDGITGVPQ PVEGVSSFMN VTLDKCTKYN
460 470 480 490 500
IYDVSGVGVI RVSNDTFLNG ITYTSTSGNL LGFKDVTKGT IYSITPCNPP
510 520 530 540 550
DQLVVYQQAV VGAMLSENFT SYGFSNVVEL PKFFYASNGT YNCTDAVLTY
560 570 580 590 600
SSFGVCADGS IIAVQPRNVS YDSVSAIVTA NLSIPSNWTT SVQVEYLQIT
610 620 630 640 650
STPIVVDCST YVCNGNVRCV ELLKQYTSAC KTIEDALRNS ARLESADVSE
660 670 680 690 700
MLTFDKKAFT LANVSSFGDY NLSSVIPSLP TSGSRVAGRS AIEDILFSKL
710 720 730 740 750
VTSGLGTVDA DYKKCTKGLS IADLACAQYY NGIMVLPGVA DAERMAMYTG
760 770 780 790 800
SLIGGIALGG LTSAVSIPFS LAIQARLNYV ALQTDVLQEN QKILAASFNK
810 820 830 840 850
AMTNIVDAFT GVNDAITQTS QALQTVATAL NKIQDVVNQQ GNSLNHLTSQ
860 870 880 890 900
LRQNFQAISS SIQAIYDRLD TIQADQQVDR LITGRLAALN VFVSHTLTKY
910 920 930 940 950
TEVRASRQLA QQKVNECVKS QSKRYGFCGN GTHIFSIVNA APEGLVFLHT
960 970 980 990 1000
VLLPTQYKDV EAWSGLCVDG TNGYVLRQPN LALYKEGNYY RITSRIMFEP
1010 1020 1030 1040 1050
RIPTMADFVQ IENCNVTFVN ISRSELQTIV PEYIDVNKTL QELSYKLPNY
1060 1070 1080 1090 1100
TVPDLVVEQY NQTILNLTSE ISTLENKSAE LNYTVQKLQT LIDNINSTLV
1110 1120 1130 1140 1150
DLKWLNRVET YIKWPWWVWL CISVVLIFVV SMLLLCCCST GCCGFFSCFA
1160 1170
SSIRGCCEST KLPYYDVEKI HIQ
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 98 | N → S in strain: Isolate LRI 281. | 1 | |
Natural varianti | 120 | N → I in strain: Isolate LRI 281. | 1 | |
Natural varianti | 127 – 128 | LR → IS in strain: Isolate A162. | 2 | |
Natural varianti | 176 | N → T in strain: Isolate P100E. | 1 | |
Natural varianti | 210 | T → S in strain: Isolate A162. | 1 | |
Natural varianti | 223 | T → N in strain: Isolate A162. | 1 | |
Natural varianti | 228 – 229 | DF → V in strain: Isolate A162. | 2 | |
Natural varianti | 230 | C → F in strain: Isolate RW Stock, Isolate P11A, Isolate P11B, Isolate P100E and Isolate ATCC VR-74. | 1 | |
Natural varianti | 230 | C → L in strain: Isolate LRI 281. | 1 | |
Natural varianti | 248 | S → A in strain: Isolate A162. | 1 | |
Natural varianti | 270 | D → Y in strain: Isolate P100E. | 1 | |
Natural varianti | 295 | V → A in strain: Isolate LRI 281. | 1 | |
Natural varianti | 300 | T → M in strain: Isolate P100E. | 1 | |
Natural varianti | 307 | D → N in strain: Isolate A162. | 1 | |
Natural varianti | 310 – 311 | PQ → LR in strain: Isolate A162. | 2 | |
Natural varianti | 314 – 324 | GGKCFNCYPAG → VGRCYNCRPAV in strain: Isolate A162. Add BLAST | 11 | |
Natural varianti | 336 | K → N in strain: Isolate LRI 281. | 1 | |
Natural varianti | 349 – 358 | KYVAVYANVG → QFVGAKFD in strain: Isolate A162. | 10 | |
Natural varianti | 401 | V → M in strain: Isolate A162. | 1 | |
Natural varianti | 404 – 411 | WAYSKYYT → LANLNSHN in strain: Isolate A162. | 8 | |
Natural varianti | 414 | S → T in strain: Isolate P100E. | 1 | |
Natural varianti | 424 | G → V in strain: Isolate A162. | 1 | |
Natural varianti | 430 | Q → K in strain: Isolate A162. | 1 | |
Natural varianti | 441 | V → A in strain: Isolate LRI 281. | 1 | |
Natural varianti | 444 | D → N in strain: Isolate A162. | 1 | |
Natural varianti | 462 | V → I in strain: Isolate A162. | 1 | |
Natural varianti | 481 | L → V in strain: Isolate A162. | 1 | |
Natural varianti | 488 | K → N in strain: Isolate A162. | 1 | |
Natural varianti | 530 | L → M in strain: Isolate A162. | 1 | |
Natural varianti | 577 | I → T in strain: Isolate P11A. | 1 | |
Natural varianti | 578 | V → G in strain: Isolate P11B. | 1 | |
Natural varianti | 590 | T → I in strain: Isolate P100E. | 1 | |
Natural varianti | 642 | R → M in strain: Isolate A162. | 1 | |
Natural varianti | 681 | T → R in strain: Isolate A162. | 1 | |
Natural varianti | 700 | L → I in strain: Isolate RW Stock, Isolate P11A, Isolate P11B and Isolate P100E. | 1 | |
Natural varianti | 711 | D → N in strain: Isolate LRI 281. | 1 | |
Natural varianti | 714 | K → N in strain: Isolate RW Stock, Isolate P11A, Isolate P11B and Isolate P100E. | 1 | |
Natural varianti | 765 | V → A in strain: Isolate A162. | 1 | |
Natural varianti | 775 | A → S in strain: Isolate A162. | 1 | |
Natural varianti | 846 | H → Y in strain: Isolate P11A and Isolate P11B. | 1 | |
Natural varianti | 871 | T → I in strain: Isolate A162. | 1 | |
Natural varianti | 937 | I → L in strain: Isolate A162. | 1 | |
Natural varianti | 971 | T → R in strain: Isolate A162. | 1 | |
Natural varianti | 1005 | M → I in strain: Isolate A162. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X16816 Genomic RNA Translation: CAA34723.1 AF304460 Genomic RNA Translation: AAG48592.1 AF344186 Genomic RNA Translation: AAK32188.1 AF344187 Genomic RNA Translation: AAK32189.1 AF344188 Genomic RNA Translation: AAK32190.1 AF344189 Genomic RNA Translation: AAK32191.1 Y09923 Genomic RNA Translation: CAA71056.1 Y10051 Genomic RNA Translation: CAA71146.1 Y10052 Genomic RNA Translation: CAA71147.1 X15654 Genomic RNA Translation: CAA33680.1 |
PIRi | A34766, VGIHHC |
RefSeqi | NP_073551.1, NC_002645.1 |
Genome annotation databases
GeneIDi | 918758 |
KEGGi | vg:918758 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X16816 Genomic RNA Translation: CAA34723.1 AF304460 Genomic RNA Translation: AAG48592.1 AF344186 Genomic RNA Translation: AAK32188.1 AF344187 Genomic RNA Translation: AAK32189.1 AF344188 Genomic RNA Translation: AAK32190.1 AF344189 Genomic RNA Translation: AAK32191.1 Y09923 Genomic RNA Translation: CAA71056.1 Y10051 Genomic RNA Translation: CAA71146.1 Y10052 Genomic RNA Translation: CAA71147.1 X15654 Genomic RNA Translation: CAA33680.1 |
PIRi | A34766, VGIHHC |
RefSeqi | NP_073551.1, NC_002645.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5YL9 | X-ray | 1.86 | A | 784-872 | [»] | |
B | 1052-1104 | [»] | ||||
5ZHY | X-ray | 2.44 | A/B/C/D/E/F | 789-856 | [»] | |
5ZUV | X-ray | 2.21 | A/B/C | 785-873 | [»] | |
6ATK | X-ray | 3.50 | D/E/F | 293-435 | [»] | |
6U7H | electron microscopy | 3.10 | A/B/C | 1-1113 | [»] | |
7CYC | electron microscopy | 3.21 | A/B/C | 1-1116 | [»] | |
7CYD | electron microscopy | 3.55 | A/B/C | 1-1116 | [»] | |
SMRi | P15423 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Proteomic databases
PRIDEi | P15423 |
Protocols and materials databases
DNASUi | 918758 |
Genome annotation databases
GeneIDi | 918758 |
KEGGi | vg:918758 |
Enzyme and pathway databases
SABIO-RKi | P15423 |
Family and domain databases
HAMAPi | MF_04200, ALPHA_CORONA_SPIKE, 1 hit |
InterProi | View protein in InterPro IPR042552, ALPHA_CORONA_SPIKE IPR043607, CoV_S1_C IPR043473, S2_sf_CoV IPR002551, Spike_S1_CoV IPR002552, Spike_S2_CoV IPR043614, Spike_S2_CoV_C IPR044873, Spike_S2_CoV_HR1 IPR044874, Spike_S2_CoV_HR2 |
Pfami | View protein in Pfam PF01600, CoV_S1, 1 hit PF19209, CoV_S1_C, 1 hit PF01601, CoV_S2, 1 hit PF19214, CoV_S2_C, 1 hit |
SUPFAMi | SSF111474, SSF111474, 2 hits |
PROSITEi | View protein in PROSITE PS51923, COV_S2_HR1, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SPIKE_CVH22 | |
Accessioni | P15423Primary (citable) accession number: P15423 Secondary accession number(s): P89342 Q990M4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1990 |
Last sequence update: | April 1, 1990 | |
Last modified: | May 25, 2022 | |
This is version 130 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families