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Protein

Rhodopsin

Gene

Rho

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth (PubMed:9020854). Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change that activates signaling via G-proteins. Subsequent receptor phosphorylation mediates displacement of the bound G-protein alpha subunit by the arrestin SAG and terminates signaling (PubMed:27353443).2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei113Plays an important role in the conformation switch to the active conformationBy similarity1
Metal bindingi201ZincBy similarity1
Metal bindingi279ZincBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionG-protein coupled receptor, Photoreceptor protein, Receptor, Retinal protein, Transducer
Biological processSensory transduction, Vision
LigandChromophore, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-2453902 The canonical retinoid cycle in rods (twilight vision)
R-MMU-2485179 Activation of the phototransduction cascade
R-MMU-2514859 Inactivation, recovery and regulation of the phototransduction cascade
R-MMU-418594 G alpha (i) signalling events
R-MMU-419771 Opsins
R-MMU-5620916 VxPx cargo-targeting to cilium

Names & Taxonomyi

Protein namesi
Recommended name:
Rhodopsin
Gene namesi
Name:Rho
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:97914 Rho

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 36ExtracellularCuratedAdd BLAST36
Transmembranei37 – 61Helical; Name=1By similarityAdd BLAST25
Topological domaini62 – 73CytoplasmicCuratedAdd BLAST12
Transmembranei74 – 96Helical; Name=2By similarityAdd BLAST23
Topological domaini97 – 110ExtracellularCuratedAdd BLAST14
Transmembranei111 – 133Helical; Name=3By similarityAdd BLAST23
Topological domaini134 – 152CytoplasmicCuratedAdd BLAST19
Transmembranei153 – 173Helical; Name=4By similarityAdd BLAST21
Topological domaini174 – 202ExtracellularCuratedAdd BLAST29
Transmembranei203 – 224Helical; Name=5By similarityAdd BLAST22
Topological domaini225 – 252CytoplasmicCuratedAdd BLAST28
Transmembranei253 – 274Helical; Name=6By similarityAdd BLAST22
Topological domaini275 – 286ExtracellularCuratedAdd BLAST12
Transmembranei287 – 308Helical; Name=7By similarityAdd BLAST22
Topological domaini309 – 348CytoplasmicCuratedAdd BLAST40

Keywords - Cellular componenti

Cell projection, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice show no response in electroretinograms at low light intensity. They fail to form rod outer segments leading to degeneration of photoreceptor cells within 3 months of birth.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001976871 – 348RhodopsinAdd BLAST348

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Glycosylationi2N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi15N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi110 ↔ 187PROSITE-ProRule annotation
Modified residuei296N6-(retinylidene)lysineBy similarity1
Lipidationi322S-palmitoyl cysteineBy similarity1
Lipidationi323S-palmitoyl cysteineBy similarity1
Modified residuei334Phosphoserine1 Publication1
Modified residuei336PhosphothreonineBy similarity1
Modified residuei338Phosphoserine1 Publication1
Modified residuei340PhosphothreonineBy similarity1
Modified residuei342PhosphothreonineBy similarity1
Modified residuei343Phosphoserine1 Publication1

Post-translational modificationi

Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.1 Publication
Contains one covalently linked retinal chromophore. Upon light absorption, the covalently bound 11-cis-retinal is converted to all-trans-retinal. After hydrolysis of the Schiff base and release of the covalently bound all-trans-retinal, active rhodopsin is regenerated by binding of a fresh molecule of 11-cis-retinal.By similarity

Keywords - PTMi

Acetylation, Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

MaxQBiP15409
PaxDbiP15409
PRIDEiP15409

PTM databases

iPTMnetiP15409
PhosphoSitePlusiP15409
SwissPalmiP15409

Expressioni

Tissue specificityi

Rod-shaped photoreceptor cells in the retina (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000030324 Expressed in 56 organ(s), highest expression level in retina
CleanExiMM_RHO
ExpressionAtlasiP15409 baseline and differential
GenevisibleiP15409 MM

Interactioni

Subunit structurei

Homodimer. Interacts (phosphorylated form) with SAG. Interacts with GNAT1, Interacts with GNAT3. SAG and G-proteins compete for a common binding site. Interacts with GRK1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiP15409, 2 interactors
MINTiP15409
STRINGi10090.ENSMUSP00000032471

Structurei

3D structure databases

ProteinModelPortaliP15409
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni330 – 348Interaction with SAGBy similarityAdd BLAST19

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi134 – 136'Ionic lock' involved in activated form stabilizationBy similarity3

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656 Eukaryota
ENOG410XRW9 LUCA
GeneTreeiENSGT00760000118977
HOGENOMiHOG000253932
HOVERGENiHBG107442
InParanoidiP15409
KOiK04250
OMAiLAAYMFM
OrthoDBiEOG091G0BDA
TreeFamiTF324998

Family and domain databases

InterProiView protein in InterPro
IPR000276 GPCR_Rhodpsn
IPR017452 GPCR_Rhodpsn_7TM
IPR001760 Opsin
IPR027430 Retinal_BS
IPR000732 Rhodopsin
IPR019477 Rhodopsin_N
PfamiView protein in Pfam
PF00001 7tm_1, 1 hit
PF10413 Rhodopsin_N, 1 hit
PRINTSiPR00237 GPCRRHODOPSN
PR00238 OPSIN
PR00579 RHODOPSIN
SMARTiView protein in SMART
SM01381 7TM_GPCR_Srsx, 1 hit
PROSITEiView protein in PROSITE
PS00237 G_PROTEIN_RECEP_F1_1, 1 hit
PS50262 G_PROTEIN_RECEP_F1_2, 1 hit
PS00238 OPSIN, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.iShow all

P15409-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNGTEGPNFY VPFSNVTGVV RSPFEQPQYY LAEPWQFSML AAYMFLLIVL
60 70 80 90 100
GFPINFLTLY VTVQHKKLRT PLNYILLNLA VADLFMVFGG FTTTLYTSLH
110 120 130 140 150
GYFVFGPTGC NLEGFFATLG GEIALWSLVV LAIERYVVVC KPMSNFRFGE
160 170 180 190 200
NHAIMGVVFT WIMALACAAP PLVGWSRYIP EGMQCSCGID YYTLKPEVNN
210 220 230 240 250
ESFVIYMFVV HFTIPMIVIF FCYGQLVFTV KEAAAQQQES ATTQKAEKEV
260 270 280 290 300
TRMVIIMVIF FLICWLPYAS VAFYIFTHQG SNFGPIFMTL PAFFAKSSSI
310 320 330 340
YNPVIYIMLN KQFRNCMLTT LCCGKNPLGD DDASATASKT ETSQVAPA
Length:348
Mass (Da):39,070
Last modified:July 27, 2011 - v2
Checksum:iB336C04B699AFF75
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0N4SUP8A0A0N4SUP8_MOUSE
Rhodopsin
Rho
203Annotation score:
A0A0N4SV48A0A0N4SV48_MOUSE
Rhodopsin
Rho
189Annotation score:
A0A0N4SWC6A0A0N4SWC6_MOUSE
Rhodopsin
Rho
54Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti20V → G in AAA39861 (PubMed:2844600).Curated1
Sequence conflicti20V → G in AAA63392 (PubMed:1978723).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36699
, M36695, M36696, M36697, M36698 Genomic DNA Translation: AAA39861.1
M55171 Genomic DNA Translation: AAA63392.1
AK044333 mRNA Translation: BAC31871.1
AK044412 mRNA Translation: BAC31908.1
CH466523 Genomic DNA Translation: EDK99545.1
BC013125 mRNA Translation: AAH13125.1
BC031766 mRNA Translation: AAH31766.1
CCDSiCCDS20446.1
PIRiA23665
RefSeqiNP_663358.1, NM_145383.1
UniGeneiMm.2965
Mm.406156

Genome annotation databases

EnsembliENSMUST00000032471; ENSMUSP00000032471; ENSMUSG00000030324
GeneIDi212541
KEGGimmu:212541
UCSCiuc009djk.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36699
, M36695, M36696, M36697, M36698 Genomic DNA Translation: AAA39861.1
M55171 Genomic DNA Translation: AAA63392.1
AK044333 mRNA Translation: BAC31871.1
AK044412 mRNA Translation: BAC31908.1
CH466523 Genomic DNA Translation: EDK99545.1
BC013125 mRNA Translation: AAH13125.1
BC031766 mRNA Translation: AAH31766.1
CCDSiCCDS20446.1
PIRiA23665
RefSeqiNP_663358.1, NM_145383.1
UniGeneiMm.2965
Mm.406156

3D structure databases

ProteinModelPortaliP15409
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP15409, 2 interactors
MINTiP15409
STRINGi10090.ENSMUSP00000032471

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiP15409
PhosphoSitePlusiP15409
SwissPalmiP15409

Proteomic databases

MaxQBiP15409
PaxDbiP15409
PRIDEiP15409

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000032471; ENSMUSP00000032471; ENSMUSG00000030324
GeneIDi212541
KEGGimmu:212541
UCSCiuc009djk.1 mouse

Organism-specific databases

CTDi6010
MGIiMGI:97914 Rho

Phylogenomic databases

eggNOGiKOG3656 Eukaryota
ENOG410XRW9 LUCA
GeneTreeiENSGT00760000118977
HOGENOMiHOG000253932
HOVERGENiHBG107442
InParanoidiP15409
KOiK04250
OMAiLAAYMFM
OrthoDBiEOG091G0BDA
TreeFamiTF324998

Enzyme and pathway databases

ReactomeiR-MMU-2453902 The canonical retinoid cycle in rods (twilight vision)
R-MMU-2485179 Activation of the phototransduction cascade
R-MMU-2514859 Inactivation, recovery and regulation of the phototransduction cascade
R-MMU-418594 G alpha (i) signalling events
R-MMU-419771 Opsins
R-MMU-5620916 VxPx cargo-targeting to cilium

Miscellaneous databases

ChiTaRSiRho mouse
PROiPR:P15409
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030324 Expressed in 56 organ(s), highest expression level in retina
CleanExiMM_RHO
ExpressionAtlasiP15409 baseline and differential
GenevisibleiP15409 MM

Family and domain databases

InterProiView protein in InterPro
IPR000276 GPCR_Rhodpsn
IPR017452 GPCR_Rhodpsn_7TM
IPR001760 Opsin
IPR027430 Retinal_BS
IPR000732 Rhodopsin
IPR019477 Rhodopsin_N
PfamiView protein in Pfam
PF00001 7tm_1, 1 hit
PF10413 Rhodopsin_N, 1 hit
PRINTSiPR00237 GPCRRHODOPSN
PR00238 OPSIN
PR00579 RHODOPSIN
SMARTiView protein in SMART
SM01381 7TM_GPCR_Srsx, 1 hit
PROSITEiView protein in PROSITE
PS00237 G_PROTEIN_RECEP_F1_1, 1 hit
PS50262 G_PROTEIN_RECEP_F1_2, 1 hit
PS00238 OPSIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiOPSD_MOUSE
AccessioniPrimary (citable) accession number: P15409
Secondary accession number(s): Q8K0D8, Q96DZ2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: July 27, 2011
Last modified: September 12, 2018
This is version 166 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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