UniProtKB - P15409 (OPSD_MOUSE)
Rhodopsin
Rho
Functioni
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 113 | Plays an important role in the conformation switch to the active conformationBy similarity | 1 | |
Metal bindingi | 201 | ZincBy similarity | 1 | |
Metal bindingi | 279 | ZincBy similarity | 1 |
GO - Molecular functioni
- 11-cis retinal binding Source: UniProtKB
- G protein-coupled photoreceptor activity Source: UniProtKB
- metal ion binding Source: UniProtKB-KW
- retinal binding Source: MGI
- spectrin binding Source: MGI
GO - Biological processi
- absorption of visible light Source: AgBase
- cellular response to light stimulus Source: MGI
- detection of light stimulus Source: MGI
- detection of temperature stimulus involved in thermoception Source: MGI
- G protein-coupled receptor signaling pathway Source: MGI
- photoreceptor cell maintenance Source: MGI
- phototransduction Source: MGI
- protein-chromophore linkage Source: UniProtKB-KW
- protein phosphorylation Source: MGI
- red, far-red light phototransduction Source: MGI
- response to light stimulus Source: MGI
- retina development in camera-type eye Source: MGI
- rhodopsin mediated signaling pathway Source: UniProtKB
- sensory perception of light stimulus Source: MGI
- thermotaxis Source: MGI
- visual perception Source: MGI
Keywordsi
Molecular function | G-protein coupled receptor, Photoreceptor protein, Receptor, Retinal protein, Transducer |
Biological process | Sensory transduction, Vision |
Ligand | Chromophore, Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-2453902, The canonical retinoid cycle in rods (twilight vision) R-MMU-2485179, Activation of the phototransduction cascade R-MMU-2514859, Inactivation, recovery and regulation of the phototransduction cascade R-MMU-418594, G alpha (i) signalling events R-MMU-419771, Opsins R-MMU-5620916, VxPx cargo-targeting to cilium |
Names & Taxonomyi
Protein namesi | Recommended name: Rhodopsin |
Gene namesi | Name:Rho |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:97914, Rho |
Subcellular locationi
Other locations
- Membrane By similarity; Multi-pass membrane protein By similarity
- photoreceptor outer segment 3 Publications
Note: Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to disk membranes in the rod outer segment (OS) photosensory cilia.By similarity
Endoplasmic reticulum
- rough endoplasmic reticulum membrane Source: MGI
Golgi apparatus
- Golgi apparatus Source: MGI
Plasma Membrane
- integral component of plasma membrane Source: GO_Central
- photoreceptor inner segment membrane Source: UniProtKB
- photoreceptor outer segment membrane Source: UniProtKB
- plasma membrane Source: UniProtKB
- sperm head plasma membrane Source: MGI
Other locations
- cell-cell junction Source: MGI
- integral component of membrane Source: UniProtKB
- photoreceptor disc membrane Source: UniProtKB
- photoreceptor inner segment Source: UniProtKB
- photoreceptor outer segment Source: MGI
- sperm midpiece Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 36 | ExtracellularCuratedAdd BLAST | 36 | |
Transmembranei | 37 – 61 | Helical; Name=1By similarityAdd BLAST | 25 | |
Topological domaini | 62 – 73 | CytoplasmicCuratedAdd BLAST | 12 | |
Transmembranei | 74 – 96 | Helical; Name=2By similarityAdd BLAST | 23 | |
Topological domaini | 97 – 110 | ExtracellularCuratedAdd BLAST | 14 | |
Transmembranei | 111 – 133 | Helical; Name=3By similarityAdd BLAST | 23 | |
Topological domaini | 134 – 152 | CytoplasmicCuratedAdd BLAST | 19 | |
Transmembranei | 153 – 173 | Helical; Name=4By similarityAdd BLAST | 21 | |
Topological domaini | 174 – 202 | ExtracellularCuratedAdd BLAST | 29 | |
Transmembranei | 203 – 224 | Helical; Name=5By similarityAdd BLAST | 22 | |
Topological domaini | 225 – 252 | CytoplasmicCuratedAdd BLAST | 28 | |
Transmembranei | 253 – 274 | Helical; Name=6By similarityAdd BLAST | 22 | |
Topological domaini | 275 – 286 | ExtracellularCuratedAdd BLAST | 12 | |
Transmembranei | 287 – 308 | Helical; Name=7By similarityAdd BLAST | 22 | |
Topological domaini | 309 – 348 | CytoplasmicCuratedAdd BLAST | 40 |
Keywords - Cellular componenti
Cell projection, MembranePathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000197687 | 1 – 348 | RhodopsinAdd BLAST | 348 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Glycosylationi | 2 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 15 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 110 ↔ 187 | PROSITE-ProRule annotation | ||
Modified residuei | 296 | N6-(retinylidene)lysineBy similarity | 1 | |
Lipidationi | 322 | S-palmitoyl cysteineBy similarity | 1 | |
Lipidationi | 323 | S-palmitoyl cysteineBy similarity | 1 | |
Modified residuei | 334 | Phosphoserine1 Publication | 1 | |
Modified residuei | 336 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 338 | Phosphoserine1 Publication | 1 | |
Modified residuei | 340 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 342 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 343 | Phosphoserine1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, PhosphoproteinProteomic databases
MaxQBi | P15409 |
PaxDbi | P15409 |
PRIDEi | P15409 |
PTM databases
GlyGeni | P15409, 2 sites |
iPTMneti | P15409 |
PhosphoSitePlusi | P15409 |
SwissPalmi | P15409 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000030324, Expressed in retinal neural layer and 89 other tissues |
ExpressionAtlasi | P15409, baseline and differential |
Genevisiblei | P15409, MM |
Interactioni
Subunit structurei
Homodimer (By similarity). May form a complex composed of RHO, GRK1 and RCVRN in a Ca2+-dependent manner; RCVRN prevents the interaction between GRK1 and RHO (By similarity).
Interacts with GRK1 (By similarity).
Interacts (phosphorylated form) with SAG (By similarity).
Interacts with GNAT1 (By similarity).
Interacts with GNAT3. SAG and G-proteins compete for a common binding site (By similarity).
Interacts with PRCD; the interaction promotes PRCD stability (PubMed:27509380).
By similarity1 PublicationGO - Molecular functioni
- spectrin binding Source: MGI
Protein-protein interaction databases
IntActi | P15409, 3 interactors |
MINTi | P15409 |
STRINGi | 10090.ENSMUSP00000032471 |
Miscellaneous databases
RNActi | P15409, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 330 – 348 | Interaction with SAGBy similarityAdd BLAST | 19 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 134 – 136 | 'Ionic lock' involved in activated form stabilizationBy similarity | 3 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3656, Eukaryota |
GeneTreei | ENSGT00960000186618 |
HOGENOMi | CLU_009579_3_0_1 |
InParanoidi | P15409 |
OMAi | EQPQYYL |
OrthoDBi | 940057at2759 |
TreeFami | TF324998 |
Family and domain databases
InterProi | View protein in InterPro IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM IPR001760, Opsin IPR027430, Retinal_BS IPR000732, Rhodopsin IPR019477, Rhodopsin_N |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit PF10413, Rhodopsin_N, 1 hit |
PRINTSi | PR00237, GPCRRHODOPSN PR00238, OPSIN PR00579, RHODOPSIN |
SMARTi | View protein in SMART SM01381, 7TM_GPCR_Srsx, 1 hit |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit PS00238, OPSIN, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MNGTEGPNFY VPFSNVTGVV RSPFEQPQYY LAEPWQFSML AAYMFLLIVL
60 70 80 90 100
GFPINFLTLY VTVQHKKLRT PLNYILLNLA VADLFMVFGG FTTTLYTSLH
110 120 130 140 150
GYFVFGPTGC NLEGFFATLG GEIALWSLVV LAIERYVVVC KPMSNFRFGE
160 170 180 190 200
NHAIMGVVFT WIMALACAAP PLVGWSRYIP EGMQCSCGID YYTLKPEVNN
210 220 230 240 250
ESFVIYMFVV HFTIPMIVIF FCYGQLVFTV KEAAAQQQES ATTQKAEKEV
260 270 280 290 300
TRMVIIMVIF FLICWLPYAS VAFYIFTHQG SNFGPIFMTL PAFFAKSSSI
310 320 330 340
YNPVIYIMLN KQFRNCMLTT LCCGKNPLGD DDASATASKT ETSQVAPA
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0N4SUP8 | A0A0N4SUP8_MOUSE | Rhodopsin | Rho | 203 | Annotation score: | ||
A0A0N4SV48 | A0A0N4SV48_MOUSE | Rhodopsin | Rho | 189 | Annotation score: | ||
A0A0N4SWC6 | A0A0N4SWC6_MOUSE | Rhodopsin | Rho | 54 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 20 | V → G in AAA39861 (PubMed:2844600).Curated | 1 | |
Sequence conflicti | 20 | V → G in AAA63392 (PubMed:1978723).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M36699 , M36695, M36696, M36697, M36698 Genomic DNA Translation: AAA39861.1 M55171 Genomic DNA Translation: AAA63392.1 AK044333 mRNA Translation: BAC31871.1 AK044412 mRNA Translation: BAC31908.1 CH466523 Genomic DNA Translation: EDK99545.1 BC013125 mRNA Translation: AAH13125.1 BC031766 mRNA Translation: AAH31766.1 |
CCDSi | CCDS20446.1 |
PIRi | A23665 |
RefSeqi | NP_663358.1, NM_145383.1 |
Genome annotation databases
Ensembli | ENSMUST00000032471; ENSMUSP00000032471; ENSMUSG00000030324 |
GeneIDi | 212541 |
KEGGi | mmu:212541 |
UCSCi | uc009djk.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M36699 , M36695, M36696, M36697, M36698 Genomic DNA Translation: AAA39861.1 M55171 Genomic DNA Translation: AAA63392.1 AK044333 mRNA Translation: BAC31871.1 AK044412 mRNA Translation: BAC31908.1 CH466523 Genomic DNA Translation: EDK99545.1 BC013125 mRNA Translation: AAH13125.1 BC031766 mRNA Translation: AAH31766.1 |
CCDSi | CCDS20446.1 |
PIRi | A23665 |
RefSeqi | NP_663358.1, NM_145383.1 |
3D structure databases
SMRi | P15409 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | P15409, 3 interactors |
MINTi | P15409 |
STRINGi | 10090.ENSMUSP00000032471 |
Protein family/group databases
GPCRDBi | Search... |
PTM databases
GlyGeni | P15409, 2 sites |
iPTMneti | P15409 |
PhosphoSitePlusi | P15409 |
SwissPalmi | P15409 |
Proteomic databases
MaxQBi | P15409 |
PaxDbi | P15409 |
PRIDEi | P15409 |
Protocols and materials databases
Antibodypediai | 17456, 522 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000032471; ENSMUSP00000032471; ENSMUSG00000030324 |
GeneIDi | 212541 |
KEGGi | mmu:212541 |
UCSCi | uc009djk.1, mouse |
Organism-specific databases
CTDi | 6010 |
MGIi | MGI:97914, Rho |
Phylogenomic databases
eggNOGi | KOG3656, Eukaryota |
GeneTreei | ENSGT00960000186618 |
HOGENOMi | CLU_009579_3_0_1 |
InParanoidi | P15409 |
OMAi | EQPQYYL |
OrthoDBi | 940057at2759 |
TreeFami | TF324998 |
Enzyme and pathway databases
Reactomei | R-MMU-2453902, The canonical retinoid cycle in rods (twilight vision) R-MMU-2485179, Activation of the phototransduction cascade R-MMU-2514859, Inactivation, recovery and regulation of the phototransduction cascade R-MMU-418594, G alpha (i) signalling events R-MMU-419771, Opsins R-MMU-5620916, VxPx cargo-targeting to cilium |
Miscellaneous databases
BioGRID-ORCSi | 212541, 1 hit in 17 CRISPR screens |
ChiTaRSi | Rho, mouse |
PROi | PR:P15409 |
RNActi | P15409, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000030324, Expressed in retinal neural layer and 89 other tissues |
ExpressionAtlasi | P15409, baseline and differential |
Genevisiblei | P15409, MM |
Family and domain databases
InterProi | View protein in InterPro IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM IPR001760, Opsin IPR027430, Retinal_BS IPR000732, Rhodopsin IPR019477, Rhodopsin_N |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit PF10413, Rhodopsin_N, 1 hit |
PRINTSi | PR00237, GPCRRHODOPSN PR00238, OPSIN PR00579, RHODOPSIN |
SMARTi | View protein in SMART SM01381, 7TM_GPCR_Srsx, 1 hit |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit PS00238, OPSIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | OPSD_MOUSE | |
Accessioni | P15409Primary (citable) accession number: P15409 Secondary accession number(s): Q8K0D8, Q96DZ2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1990 |
Last sequence update: | July 27, 2011 | |
Last modified: | February 10, 2021 | |
This is version 182 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - 7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries